Sexual
Gonadorelin
Hormonal
10
Amino acids
Molecular weight
Peptide
Type
Gonadorelin is a synthetic decapeptide identical to endogenous gonadotropin-releasing hormone (GnRH), which stimulates the anterior pituitary to secrete luteinizing hormone (LH) and follicle-stimulating hormone (FSH). It is used clinically in pulsatile pump delivery to treat hypogonadotropic hypogonadism, functional hypothalamic amenorrhea, and infertility, and is increasingly used subcutaneously as a TRT adjunct to preserve testicular function. Originally FDA-approved under the brand names Factrel and Lutrepulse, both US human products were withdrawn from the market; gonadorelin remains approved internationally and in veterinary medicine, and is widely compounded for human use.
Top researched benefits
Overview of Gonadorelin
Gonadorelin binds the GnRH type I receptor (GnRHR), a Gq/G11-coupled GPCR on pituitary gonadotroph cells, activating phospholipase C-beta and triggering IP3-mediated calcium release and PKC activation, driving pulsatile LH and FSH secretion. Pulsatile administration preserves receptor sensitivity and sustains gonadal steroidogenesis, while continuous exposure causes receptor downregulation and paradoxical hormonal suppression.
fertility and reproductive
- Treatment of conditions like Kallmann syndrome where GnRH deficiency causes low testosterone and infertility.
- Induces ovulation in women with hypothalamic amenorrhea who don't ovulate regularly.
- Stimulates testosterone and sperm production by restoring LH/FSH pulsatility.
- Treatment of delayed puberty due to hypothalamic-pituitary dysfunction.
diagnostic
- Used to assess hypothalamic-pituitary function and diagnose GnRH deficiency.
- Evaluates pituitary reserve and responsiveness to GnRH stimulation.
hormone optimization
- Maintains testicular function and fertility during testosterone replacement therapy.
- Helps restore natural testosterone production after anabolic steroid use.
- Treatment of undescended testes in pediatric patients.
Typical Dose
100mcg per subcutaneous injection (TRT adjunct protocol)
Frequency
twice daily (subcutaneous TRT adjunct); every 90–120 minutes via infusion pump (clinical pulsatile fertility protocol using 5–20mcg per pulse)
Cycle Duration
8 weeks with reassessment; ongoing alongside TRT use
Storage
Lyophilized: store at 2–8°C or -20°C long-term, sealed and dry. Reconstituted: refrigerate at 2–8°C and use within 28 days; do not freeze after reconstitution.
Chemical Makeup
Key benefits
Restores endogenous LH and FSH secretion in men with hypogonadotropic hypogonadism, stimulating spermatogenesis significantly faster than gonadotropin therapy (median 6 vs 14 months to sperm production in clinical studies)
Preserves testicular volume and sperm production in men on TRT by maintaining pituitary-gonadal axis signaling during exogenous testosterone suppression
Induces ovulation in women with functional hypothalamic amenorrhea when delivered via pulsatile pump, restoring menstrual cycles without the hyperstimulation risk associated with gonadotropin therapy
Functions as a diagnostic agent to assess hypothalamic-pituitary axis integrity — an absent or blunted LH/FSH response to a 100 mcg bolus confirms pituitary gonadotroph insufficiency
Community interest
This peptide is still gaining traction in the community.
GnRH Agonist | Fertility, Hypogonadism & TRT Adjunct
This overview is informational and based on aggregated descriptions from studies and user reports.
Was it helpful?YesNoGonadorelin Molecular Information
View the scientifc details of Gonadorelin.
10
Amino Acids
Gonadorelin
?
?
Position 1
His
His
Position 2
Trp
Trp
Position 3
Ser
Ser
Position 4
Tyr
Tyr
Position 5
Gly
Gly
Position 6
Leu
Leu
Position 7
Arg
Arg
Position 8
Pro
Pro
Position 9
Gly
Gly
Position 10
Molecular Weight
1182.31DaChain Length
10Amino AcidsType
PeptideGonadorelin Protocols
Subcutaneous injection is the primary delivery route for Gonadorelin, mimicking the pulsatile GnRH signaling of the hypothalamus to stimulate LH and FSH release from the pituitary. Intravenous administration is used clinically for diagnostic pituitary function testing. SubQ injections should be administered on an empty stomach, rotating sites across the abdomen, thighs, and upper arms.
| Goal | Dosage | Frequency | Route |
|---|---|---|---|
| TRT Support / Testicular Maintenance | 100 | 3 week range | SubQ |
| Post-Cycle Therapy (PCT) — Loading Phase | 200 | 1 day range | SubQ |
| Post-Cycle Therapy (PCT) — Maintenance Phase | 100 | 3 week range | SubQ |
| Hypogonadism / Hypothalamic Dysfunction | 5 | 16 day range | SubQ via pulsatile pump every 90 minutes |
| Diagnostic Pituitary Function Testing | 100 | 1 period | SubQ or IV (single dose) |
| Fertility Support / Amenorrhea | 5 | 16 day range | SubQ or IV via pulsatile pump (21-day cycle) |
Reconstitution Instructions
Materials needed:
Steps to reconstitute
- Draw 2.0 mL of bacteriostatic water into a sterile syringe
- Insert the needle through the vial stopper at an angle and inject the water slowly down the inside wall of the vial — never directly onto the powder
- Gently swirl or roll the vial between your palms until the powder is fully dissolved — do not shake
- Label the vial with the date and concentration (1 mg/mL = 1000 mcg/mL for a 2 mg vial)
- On a U-100 insulin syringe, 0.01 mL = 10 mcg; draw the appropriate volume for your target dose
- Clean the injection site with an alcohol swab and allow it to dry before inserting the needle
- Insert the needle at a 45–90 degree angle into subcutaneous tissue; do not aspirate
- Inject slowly and steadily, then withdraw the needle and apply light pressure
- Rotate injection sites systematically across the abdomen, thighs, and upper arms to avoid lipohypertrophy
- Store reconstituted vial refrigerated at 2–8°C and use within 4 weeks; avoid freeze-thaw cycles
Gonadorelin Cycle
The Gonadorelin Cycle section explains how long a typical cycle lasts and what to expect during each phase. Over time, your body can become less responsive with continuous use.
Taking breaks between cycles may help maintain effectiveness and support better overall results.
- Week 0-0
- LH and FSH release begins
- Week 0-0
- Testosterone increase in response to LH
- Week 1-2
- Improved hormonal markers on blood tests
- Week 2-4
- Restoration of natural hormone pulsatility
- Week 0-0
- Maintained testicular function and fertility
Dosing tools
Gonadorelin Peptide Dosage Calculator
Calculate peptide doses with our visual syringe guide.
mg
Enter the total amount of peptide in the vial in milligrams (as stated on the label).
The dose you want to inject per administration, in mcg or mg.
1,000 mcg = 1 mg
1 mL
2 mL
3 mL
5 mL
Custom
Volume of bacteriostatic water you add to reconstitute the powder. Use BAC water for preservation.
Injection Results
Based on your vial and dilution inputs.
Gonadorelin
SINGLE COMPOUNDVolume per injection
0.05
mL
Concentration
10.00
mg/mL
Doses per vial
20
doses
Total injections per vial
20 injections
How it works
Based on a 10 mg Gonadorelin vial diluted with 1 mL of bacteriostatic water, each 500 mcg injection equals 0.05 mL.
1mL / 100 units
5 units
0.050 mL
Reference Guide
Dosing Cycle
- Peptide
- Gonadorelin
- Dosing
- 100mcg per subcutaneous injection (TRT adjunct protocol)
- Dosing Frequency
- twice daily (subcutaneous TRT adjunct); every 90–120 minutes via infusion pump (clinical pulsatile fertility protocol using 5–20mcg per pulse)
- Cycle Duration
- 8 weeks with reassessment; ongoing alongside TRT use
- Storage
- Lyophilized: store at 2–8°C or -20°C long-term, sealed and dry. Reconstituted: refrigerate at 2–8°C and use within 28 days; do not freeze after reconstitution.
Note: Triple agonist; microdose for fewer side effects
Reconstitution Tips
- Use bacteriostatic water (BAC) — contains 0.9% benzyl alcohol for preservation
- Inject water slowly — aim down the vial wall, not directly onto powder
- Never shake — gently swirl or roll the vial until dissolved
- Store properly — refrigerate at 2-8°C after reconstitution
- Use within 28 days — most reconstituted peptides remain stable for about 4 weeks
- Keep sterile — always clean vial tops with alcohol before drawing
Peptide Interactions
Research suggestions of Gonadorelin interactions with other common peptides and substances.
Sexual
kis
Hormonal
Kisspeptin
SYNERGISTIC
Kisspeptin stimulates GnRH release; can work together for hormone optimization.
Side effects
Contraindications
Hormone-sensitive tumors
Pregnancy
Hypersensitivity to GnRH or analogs
Conditions worsened by sex hormone release
Stop signs
Signs of allergic reaction (rash, difficulty breathing)
Severe headaches
Unusual abdominal pain
Bad signs
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Frequently asked questions
What is a peptide dosage calculator?
A peptide dosage calculator is a free tool that converts your vial size, bacteriostatic water volume, and target dose into an exact syringe draw volume. Instead of doing the reconstitution math by hand, you enter three inputs and instantly get the concentration of your solution and how many milliliters or syringeunits to draw. This calculator works for single peptide compounds and multi-peptide blends.
How do I calculate peptide dosage from a vial?
To calculate your peptide dose, divide the total peptide content of your vial in micrograms by the volume of bacteriostatic water you added in milliliters. This gives you your solution concentration in mcg/mL. Then divide your target dose by that concentration to get your draw volume. For example, a 5mg (5,000 mcg) vial reconstituted with 2mL of BAC water gives a concentration of 2,500 mcg/mL. A 250 mcg dose would require drawing 0.1mL. This calculator automates all of those steps instantly.
How much Bacteriostatic water should I add to a peptide vial?
Most people add 2mL to 3mL of bacteriostatic water per vial, but the right amount depends on the dose you want to draw and the syringe size you are using. Adding 1mL to a 5mg vial gives you a concentration of 5,000 mcg/mL, making each dose very small in volume. Adding 2mL gives you 2,500 mcg/mL, which is easier to measure on a standard insulin syringe. A general guideline is to choose a volume that puts your typical dose somewhere between 10 and 30 units on a U-100 syringe. Use the calculator above to test different water volumes and find what works for your dose.
How are peptides different from proteins?
Both are made of amino acids, but peptides are much smaller than proteins. Because of their tiny size, peptides can act like tiny messengers in the body, sending specific signals to your cells to tell them exactly what to do.
GonadorelinResearch References
Gonadorelin is an approved compound
Gonadorelin
Gonadorelin is an approved compound
Gonadorelin in Hypogonadotropic Hypogonadism
Pulsatile GnRH administration effectively restores fertility in patients with hypothalamic hypogonadism.
n.d.
GnRH Pulsatility and Reproductive Function
Pulsatile GnRH secretion (every 60-120 min) is essential for proper FSH/LH release and reproductive function.
n.d.
Gonadorelin for Ovulation Induction
Effective treatment for ovulation induction in women with hypothalamic amenorrhea.
n.d.
Calculate peptide dosages
Learning how to calculate a peptide dose? Use our beginner-friendly peptide dosage, blend, and accumulation calculators. Enter vial size, reconstitution volume, and target dose to get exact draw volumes instantly. No guesswork, just clear guidance that helps prevent common mistakes.
