Longevity
NAD+
Metabolic
5
Amino acids
Molecular weight
Peptide
Type
NAD+ (Nicotinamide Adenine Dinucleotide) is a dinucleotide coenzyme — not a true peptide — found in every living cell and essential for mitochondrial energy production, DNA repair, and cellular signaling. Its levels decline significantly with age, and direct administration via IV infusion or subcutaneous injection is used to restore intracellular NAD+ pools. It is explored in longevity medicine, metabolic health, and neurodegenerative disease research, though clinical evidence for direct NAD+ administration remains early-stage.
Top researched benefits
Overview of NAD+
NAD+ serves as a critical co-substrate for sirtuins (SIRT1–7) and poly(ADP-ribose) polymerases (PARPs), enzymes that regulate DNA repair, gene expression, and metabolic adaptation. It also functions as an electron carrier in the mitochondrial electron transport chain, accepting hydride ions during glycolysis and the TCA cycle to drive ATP synthesis via oxidative phosphorylation.
energy and metabolism
- Restores NAD+ levels for optimal ATP synthesis
- Enhances glucose and fat metabolism
- Improves oxygen utilization and endurance
antiaging
- Activates PARP enzymes for genomic stability
- Supports longevity pathways
- May help clear senescent cells
neurological
- Improves mental clarity and focus
- Supports neuronal health and function
Typical Dose
50–200mg per subcutaneous injection (or 250–500mg per IV session)
Frequency
2–3 times per week (SubQ); weekly to monthly maintenance (IV)
Cycle Duration
6–8 week loading phase, then maintenance as needed
Storage
Lyophilized powder: room temperature (20–25°C), protected from light and moisture; long-term at -20°C. Reconstituted solution: 2–8°C for up to 28 days, protected from light.
Chemical Makeup
Key benefits
Restores declining intracellular NAD+ levels associated with aging, directly fueling sirtuin-mediated DNA repair and epigenetic regulation
Enhances mitochondrial function and ATP production, improving cellular energy output in muscle and neuronal tissue
Activates SIRT1 and SIRT3 to reduce oxidative stress and mitochondrial protein hyperacetylation in cardiac and skeletal muscle
Provides co-substrate for PARP1-mediated DNA damage response, enabling repair of strand breaks and reducing accumulation of genomic mutations linked to aging
Community interest
This peptide is still gaining traction in the community.
Coenzyme Replenishment | Longevity & Cellular Energy
This overview is informational and based on aggregated descriptions from studies and user reports.
Was it helpful?YesNoNAD+ Molecular Information
View the scientifc details of NAD+.
5
Amino Acids
NAD+
Cys
Cys
Position 1
His
His
Position 2
Asn
Asn
Position 3
Pyl
Pyl
Position 4
Pro
Pro
Position 5
Molecular Weight
663.43DaChain Length
5Amino AcidsType
PeptideNAD+ Protocols
NAD+ is most commonly administered via subcutaneous injection for at-home use or intravenous infusion in clinical settings. SubQ injections into the abdomen or upper arm are preferred for daily or multi-weekly maintenance protocols, while IV infusion (250–1000 mg over 2–4 hours) is used for loading phases, addiction recovery, and acute cognitive support.
| Goal | Dosage | Frequency | Route |
|---|---|---|---|
| Sustain baseline cellular NAD+ levels for ongoing mitochondrial function, immune support, and healthy aging | 100 | 3 week range | SubQ |
| Rapidly replete severely depleted cellular NAD+ stores over 7–10 consecutive days before transitioning to maintenance dosing | 200 | 1 day range | SubQ |
| Accelerate post-exercise muscle repair and reduce oxidative stress by supporting mitochondrial respiration and NAD+-dependent antioxidant pathways | 300 | 3 week range | SubQ |
| Support sirtuin-mediated neuroprotection and improve mental clarity and focus through sustained elevation of brain NAD+ levels | 150 | 2 week range | SubQ |
| Blunt withdrawal symptoms and support neurotransmitter homeostasis during medically supervised detoxification from alcohol or opioids | 750 | 1 day range | IV infusion (clinic only) |
| Activate PARP1-mediated DNA repair and sirtuin pathways to slow age-related NAD+ decline and support cellular resilience | 50 | 3 week range | SubQ |
Reconstitution Instructions
Materials needed:
Steps to reconstitute
- Obtain a 500 mg lyophilized NAD+ vial and a vial of bacteriostatic water (BAC water)
- Draw 3.0 mL of BAC water into a 3 mL syringe using an 18–21 gauge drawing needle — this yields a concentration of approximately 166.7 mg/mL
- Insert the drawing needle through the rubber stopper of the NAD+ vial at an angle
- Inject the BAC water slowly down the inner wall of the vial — never aim directly at the powder
- Remove the syringe and gently swirl the vial until the powder fully dissolves — do not shake
- Inspect the solution; it should be clear and colorless — discard if cloudy or particulate matter is visible
- Draw your prescribed dose into a fresh insulin syringe using the correct volume for your concentration
- Select an injection site (abdomen, thigh, or upper arm); rotate sites with each injection to prevent tissue irritation
- Clean the site with an alcohol swab and allow it to dry for 30 seconds
- Pinch the skin, insert the needle at a 45-degree angle, and depress the plunger slowly over 5–10 seconds
- Do not aspirate for subcutaneous injections; withdraw the needle smoothly and apply gentle pressure with a swab
- Label the reconstituted vial with the date opened, store refrigerated at 2–8°C, and use within 30 days
NAD+ Cycle
The NAD+ Cycle section explains how long a typical cycle lasts and what to expect during each phase. Over time, your body can become less responsive with continuous use.
Taking breaks between cycles may help maintain effectiveness and support better overall results.
- Week 1-2
- Initial energy improvements and mental clarity
- Week 3-4
- Enhanced physical performance and recovery
- Week 5-8
- Sustained energy levels and improved sleep quality
- Week 9-12
- Optimized metabolic function and cognitive benefits
Dosing tools
NAD+ Peptide Dosage Calculator
Calculate peptide doses with our visual syringe guide.
mg
Enter the total amount of peptide in the vial in milligrams (as stated on the label).
The dose you want to inject per administration, in mcg or mg.
1,000 mcg = 1 mg
1 mL
2 mL
3 mL
5 mL
Custom
Volume of bacteriostatic water you add to reconstitute the powder. Use BAC water for preservation.
Injection Results
Based on your vial and dilution inputs.
NAD+
SINGLE COMPOUNDVolume per injection
0.05
mL
Concentration
10.00
mg/mL
Doses per vial
20
doses
Total injections per vial
20 injections
How it works
Based on a 10 mg NAD+ vial diluted with 1 mL of bacteriostatic water, each 500 mcg injection equals 0.05 mL.
1mL / 100 units
5 units
0.050 mL
Reference Guide
Dosing Cycle
- Peptide
- NAD+
- Dosing
- 50–200mg per subcutaneous injection (or 250–500mg per IV session)
- Dosing Frequency
- 2–3 times per week (SubQ); weekly to monthly maintenance (IV)
- Cycle Duration
- 6–8 week loading phase, then maintenance as needed
- Storage
- Lyophilized powder: room temperature (20–25°C), protected from light and moisture; long-term at -20°C. Reconstituted solution: 2–8°C for up to 28 days, protected from light.
Note: Triple agonist; microdose for fewer side effects
Reconstitution Tips
- Use bacteriostatic water (BAC) — contains 0.9% benzyl alcohol for preservation
- Inject water slowly — aim down the vial wall, not directly onto powder
- Never shake — gently swirl or roll the vial until dissolved
- Store properly — refrigerate at 2-8°C after reconstitution
- Use within 28 days — most reconstituted peptides remain stable for about 4 weeks
- Keep sterile — always clean vial tops with alcohol before drawing
Peptide Interactions
Research suggestions of NAD+ interactions with other common peptides and substances.
Weight
ipa
Growth
Ipamorelin
SYNERGISTIC
Growth hormone pathways benefit from improved energy metabolism
Side effects
Contraindications
IV administration requires medical supervision
Avoid alcohol during treatment
Maintain consistent dosing schedule
Stop signs
Severe injection site reactions or infection signs
Persistent nausea or gastrointestinal distress
Unusual fatigue or weakness
Allergic reactions (rash, swelling, breathing difficulty)
Bad signs
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Frequently asked questions
What is a peptide dosage calculator?
A peptide dosage calculator is a free tool that converts your vial size, bacteriostatic water volume, and target dose into an exact syringe draw volume. Instead of doing the reconstitution math by hand, you enter three inputs and instantly get the concentration of your solution and how many milliliters or syringeunits to draw. This calculator works for single peptide compounds and multi-peptide blends.
How do I calculate peptide dosage from a vial?
To calculate your peptide dose, divide the total peptide content of your vial in micrograms by the volume of bacteriostatic water you added in milliliters. This gives you your solution concentration in mcg/mL. Then divide your target dose by that concentration to get your draw volume. For example, a 5mg (5,000 mcg) vial reconstituted with 2mL of BAC water gives a concentration of 2,500 mcg/mL. A 250 mcg dose would require drawing 0.1mL. This calculator automates all of those steps instantly.
How much Bacteriostatic water should I add to a peptide vial?
Most people add 2mL to 3mL of bacteriostatic water per vial, but the right amount depends on the dose you want to draw and the syringe size you are using. Adding 1mL to a 5mg vial gives you a concentration of 5,000 mcg/mL, making each dose very small in volume. Adding 2mL gives you 2,500 mcg/mL, which is easier to measure on a standard insulin syringe. A general guideline is to choose a volume that puts your typical dose somewhere between 10 and 30 units on a U-100 syringe. Use the calculator above to test different water volumes and find what works for your dose.
How are peptides different from proteins?
Both are made of amino acids, but peptides are much smaller than proteins. Because of their tiny size, peptides can act like tiny messengers in the body, sending specific signals to your cells to tell them exactly what to do.
NAD+Research References
NAD+ is an experimental compound
NAD+
NAD+ is an experimental compound
NAD+ Restoration Strategies in Clinical Practice
Review of clinical applications and restoration strategies
n.d.
Clinical Evidence for NAD+ Therapeutic Applications
Evidence for anti-aging and therapeutic applications
n.d.
NAD+ Precursor Safety and Efficacy Meta-Analysis
Meta-analysis of safety and efficacy data
n.d.
Intranasal NAD+ Delivery for Neurological Applications
Demonstrated efficacy of intranasal delivery for brain access
n.d.
Calculate peptide dosages
Learning how to calculate a peptide dose? Use our beginner-friendly peptide dosage, blend, and accumulation calculators. Enter vial size, reconstitution volume, and target dose to get exact draw volumes instantly. No guesswork, just clear guidance that helps prevent common mistakes.
