Longevity
Vilon
Immunity
2
Amino acids
Molecular weight
Peptide
Type
Vilon (Lys-Glu, KE) is a synthetic dipeptide bioregulator developed by Professor Vladimir Khavinson at the St. Petersburg Institute of Bioregulation and Gerontology. It acts as an epigenetic regulator targeting the neuroendocrine and immune systems by reactivating silenced genes in aging cells through chromatin decondensation. Used in longevity and immune-support research contexts, particularly for age-related thymic atrophy and immunosenescence.
Top researched benefits
Overview of Vilon
Vilon induces deheterochromatinization — the progressive loosening of condensed facultative heterochromatin in aging cells — restoring transcriptional access to silenced gene loci. It modulates NF-kB signaling via the CHUK gene and influences expression of longevity-associated genes including TERT, FOXO1, and IGF1, while also upregulating IL-2 production in lymphocytes to support T-cell maturation.
immune support
- Stimulates thymocyte proliferation and enlarges thymic lobules.
- Activates T-helper cells and improves immune response.
- Restores chromatin structure and gene expression in aged lymphocytes.
antiaging
- Long-term treatment increased mean lifespan 20-40% in rodent studies.
- Suppresses development of spontaneous and induced tumors.
- Promotes recovery in tissues exposed to radiation damage.
gi health
- Restores brush border membrane enzyme levels in aged animals.
- Enhances intestinal barrier function in aged rats.
Typical Dose
100–200 mcg per injection
Frequency
once daily
Cycle Duration
10-day course every 3–6 months
Storage
Lyophilized: store at -20°C long-term or 2–8°C short-term, protected from light and moisture. Reconstituted: refrigerate at 2–8°C for up to 28–30 days; do not freeze.
Chemical Makeup
Key benefits
Stimulates thymocyte proliferation and T-cell maturation, counteracting age-related thymic atrophy
Upregulates IL-2 expression in lymphocytes, enhancing cellular immune responsiveness in aged models
Reduces inflammatory cytokine levels by up to 6-fold via NF-kB pathway modulation, targeting inflammaging
Reactivates silenced chromatin in aging cells through deheterochromatinization, restoring gene expression programs
Community interest
This peptide is still gaining traction in the community.
Khavinson Dipeptide | Immune Regulation & Epigenetic Anti-Aging
This overview is informational and based on aggregated descriptions from studies and user reports.
Was it helpful?YesNoVilon Molecular Information
View the scientifc details of Vilon.
2
Amino Acids
Vilon
Lys
Lys
Position 1
Glu
Glu
Position 2
Molecular Weight
275.3DaChain Length
2Amino AcidsType
PeptideVilon Protocols
Subcutaneous injection is the primary and most bioavailable route for Vilon, administered once daily for 10 consecutive days per course. Morning administration on an empty stomach is preferred to align with peak immune and thymic bioregulatory activity; courses are repeated every 3–6 months for maintenance.
| Goal | Dosage | Frequency | Route |
|---|---|---|---|
| Immune Support / Thymic Recovery | 5 | 1 day range | SubQ |
| Anti-Aging / Longevity Maintenance | 5 | 1 day range | SubQ |
| Intensive Immune Compromise or Advanced Age | 10 | 1 day range | SubQ |
| Gene Expression / Chromatin Modulation | 5 | 2 week range | SubQ |
| Autoimmune / Type 1 Diabetes Research | 10 | 1 day range | SubQ |
| Conservative Introductory Protocol | 1 | 1 day range | SubQ |
Reconstitution Instructions
Materials needed:
Steps to reconstitute
- Wipe the rubber stopper of the Vilon vial with an alcohol swab and allow it to air dry
- Draw 2 mL of bacteriostatic water into a syringe — this produces a 10 mg/mL concentration for a 20 mg vial
- Insert the needle into the vial and inject bacteriostatic water slowly down the inside glass wall — never directly onto the powder
- Gently swirl the vial until the powder is fully dissolved — do not shake
- The reconstituted solution should be clear and colorless; discard if cloudy or particulate
- Label the vial with the reconstitution date and store in the refrigerator at 2–8°C; use within 14 days
- To draw a dose, wipe the stopper with an alcohol swab, insert the syringe, and withdraw the calculated volume (e.g., 0.5 mL = 5 mg at 10 mg/mL)
- For low introductory doses of 1 mg or less, prepare a secondary dilution: draw 0.1 mL (1 mg) from the 10 mg/mL vial and add 0.9 mL bacteriostatic water to a separate sterile vial, yielding a 1 mg/mL working solution from which doses as small as 0.1 mL (100 mcg) are easily drawable
- Clean the injection site — abdomen 2 inches from navel, front thigh, or upper arm — with an alcohol swab and allow to air dry
- Pinch a fold of skin, insert the needle at a 45-degree angle into subcutaneous tissue, and inject slowly over 5–10 seconds
- Withdraw the needle, apply gentle pressure with a clean swab, and rotate injection sites across the 10-day course
- Dispose of used needles and syringes in a sharps container
Vilon Cycle
The Vilon Cycle section explains how long a typical cycle lasts and what to expect during each phase. Over time, your body can become less responsive with continuous use.
Taking breaks between cycles may help maintain effectiveness and support better overall results.
- Week 0-0
- Immune activation and gene expression modulation begins
- Week 0-0
- Effects persist due to epigenetic changes
- Week 0-0
- Immune function improvements
- Week 0-0
- Cumulative benefits; longevity effects in animal models
Dosing tools
Vilon Peptide Dosage Calculator
Calculate peptide doses with our visual syringe guide.
mg
Enter the total amount of peptide in the vial in milligrams (as stated on the label).
The dose you want to inject per administration, in mcg or mg.
1,000 mcg = 1 mg
1 mL
2 mL
3 mL
5 mL
Custom
Volume of bacteriostatic water you add to reconstitute the powder. Use BAC water for preservation.
Injection Results
Based on your vial and dilution inputs.
Vilon
SINGLE COMPOUNDVolume per injection
0.05
mL
Concentration
10.00
mg/mL
Doses per vial
20
doses
Total injections per vial
20 injections
How it works
Based on a 10 mg Vilon vial diluted with 1 mL of bacteriostatic water, each 500 mcg injection equals 0.05 mL.
1mL / 100 units
5 units
0.050 mL
Reference Guide
Dosing Cycle
- Peptide
- Vilon
- Dosing
- 100–200 mcg per injection
- Dosing Frequency
- once daily
- Cycle Duration
- 10-day course every 3–6 months
- Storage
- Lyophilized: store at -20°C long-term or 2–8°C short-term, protected from light and moisture. Reconstituted: refrigerate at 2–8°C for up to 28–30 days; do not freeze.
Note: Triple agonist; microdose for fewer side effects
Reconstitution Tips
- Use bacteriostatic water (BAC) — contains 0.9% benzyl alcohol for preservation
- Inject water slowly — aim down the vial wall, not directly onto powder
- Never shake — gently swirl or roll the vial until dissolved
- Store properly — refrigerate at 2-8°C after reconstitution
- Use within 28 days — most reconstituted peptides remain stable for about 4 weeks
- Keep sterile — always clean vial tops with alcohol before drawing
Peptide Interactions
Research suggestions of Vilon interactions with other common peptides and substances.
Longevity
cry
Immunity
Crystagen
SYNERGISTIC
Both have immunoprotecting properties; different mechanisms.
Longevity
epi
Immunity
Epitalon
SYNERGISTIC
Often combined in comprehensive anti-aging Khavinson protocols.
Longevity
thy
Immunity
Thymalin
SYNERGISTIC
Both target thymus/immune system; complementary mechanisms.
Longevity
thym
Immunity
Thymogen
COMPATIBLE
Related immune peptides; can be used in comprehensive protocols.
Side effects
Contraindications
Active autoimmune flares (consult physician)
Known hypersensitivity
Pregnancy or breastfeeding
Stop signs
Allergic reactions
Unusual immune symptoms
Bad signs
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Frequently asked questions
What is a peptide dosage calculator?
A peptide dosage calculator is a free tool that converts your vial size, bacteriostatic water volume, and target dose into an exact syringe draw volume. Instead of doing the reconstitution math by hand, you enter three inputs and instantly get the concentration of your solution and how many milliliters or syringeunits to draw. This calculator works for single peptide compounds and multi-peptide blends.
How do I calculate peptide dosage from a vial?
To calculate your peptide dose, divide the total peptide content of your vial in micrograms by the volume of bacteriostatic water you added in milliliters. This gives you your solution concentration in mcg/mL. Then divide your target dose by that concentration to get your draw volume. For example, a 5mg (5,000 mcg) vial reconstituted with 2mL of BAC water gives a concentration of 2,500 mcg/mL. A 250 mcg dose would require drawing 0.1mL. This calculator automates all of those steps instantly.
How much Bacteriostatic water should I add to a peptide vial?
Most people add 2mL to 3mL of bacteriostatic water per vial, but the right amount depends on the dose you want to draw and the syringe size you are using. Adding 1mL to a 5mg vial gives you a concentration of 5,000 mcg/mL, making each dose very small in volume. Adding 2mL gives you 2,500 mcg/mL, which is easier to measure on a standard insulin syringe. A general guideline is to choose a volume that puts your typical dose somewhere between 10 and 30 units on a U-100 syringe. Use the calculator above to test different water volumes and find what works for your dose.
How are peptides different from proteins?
Both are made of amino acids, but peptides are much smaller than proteins. Because of their tiny size, peptides can act like tiny messengers in the body, sending specific signals to your cells to tell them exactly what to do.
VilonResearch References
Vilon is a preclinical compound
Vilon
Vilon is a preclinical compound
Peptide Bioregulators Increase Lifespan
Long-term treatment with peptide preparations including Vilon increased mean lifespan 20-40% and suppressed tumorigenesis.
n.d.
Vilon Effects on Aged Lymphocytes
Vilon improved lymphocytes from old people by restoring chromatin structure and gene expression.
n.d.
Peptides Regulating Proliferative Activity in THP-1 Cells
Vilon and other Khavinson peptides modulate key proliferative patterns and tyrosine phosphorylation of kinases.
n.d.
Short Peptide Immunoprotecting Properties
Vilon and related peptides possess age-associated immunoprotecting properties in the spleen.
n.d.
Calculate peptide dosages
Learning how to calculate a peptide dose? Use our beginner-friendly peptide dosage, blend, and accumulation calculators. Enter vial size, reconstitution volume, and target dose to get exact draw volumes instantly. No guesswork, just clear guidance that helps prevent common mistakes.
