Longevity
Thymalin
Immunity
0
Amino acids
Molecular weight
Blend
Type
Thymalin is a heterogeneous polypeptide extract derived from the thymus glands of young calves, classified as a peptide bioregulator with no single defined molecular weight. Registered as a pharmaceutical drug in Russia and Eastern Europe since 1982, it has been used clinically across those regions for over four decades in immunodeficiency, infectious disease, oncology support, and age-related immune decline. It restores immune competence through T-lymphocyte differentiation, cytokine modulation, and gene expression regulation by its short constituent peptides.
Top researched benefits
Overview of Thymalin
Thymalin's short constituent peptides—primarily the dipeptides EW (thymogen) and KE (vilon) and the tripeptide EDP—bind to double-stranded DNA and histone proteins to regulate transcription of immune-related genes governing T-cell differentiation (CD4+/CD8+ ratio), cytokine synthesis (TNF-α, IL-6, IL-2), and hematopoietic stem cell proliferation, normalizing thymus-dependent immune responses.
immune support
- Stimulates T-lymphocyte differentiation and activity for comprehensive immune system restoration.
- Accelerates recovery of lymphocyte populations including CD4+, CD3+HLA-DR+, B-cells, and NK-cells.
- 2.0-2.4 fold reduction in acute respiratory disease incidence in elderly patients.
longevity
- Clinical studies showed 2.0-2.1 fold mortality reduction over 6-8 years in treated elderly.
- Normalizes basic body functions and improves cardiovascular, endocrine, immune, and nervous systems.
- When combined with Epithalamin for 6 years, mortality decreased 4.1 times vs control.
therapeutic applications
- Faster reversal of lymphopenia and immune marker recovery in severe COVID-19 patients.
- Tumor growth arrest and regression observed in over half of animals in sarcoma studies.
- Normalizes thymus function during aging process.
Typical Dose
10mg per injection
Frequency
once daily
Cycle Duration
5–10 consecutive days, repeated 1–4 times per year
Storage
Lyophilized: store desiccated at -18°C or below, protected from light; stable 12–24 months. Reconstituted: refrigerate at 2–8°C, use within 7–28 days; avoid freeze-thaw cycles.
No visual available
Due to this peptide having no amino acids, there is no molecular chain to display.
Chemical Makeup
Key benefits
Stimulates differentiation of naive T-lymphocytes into functional CD4+ helper and CD8+ cytotoxic cells, restoring cell-mediated immunity in immunocompromised individuals
Reduced mortality by approximately half in a randomized trial of severe COVID-19 older patients when added to standard therapy, alongside 2–2.4x recovery of T-, B-, and NK-cell counts
Downregulates pro-inflammatory cytokines TNF-α and IL-6 while preserving protective interferon-gamma and IL-2 levels, benefiting chronic inflammatory and autoimmune conditions
Reduces incidence of acute respiratory infections in elderly subjects by 2.0–2.4x in controlled Russian clinical studies conducted over multi-year follow-up periods
Community interest
This peptide is still gaining traction in the community.
Thymic Peptide Complex | Immune Regulation & Longevity
This overview is informational and based on aggregated descriptions from studies and user reports.
Was it helpful?YesNoThymalin Molecular Information
View the scientifc details of Thymalin.
Molecular Weight
333.34DaChain Length
0Amino AcidsType
BlendThymalin Protocols
Subcutaneous or intramuscular injection is the primary and only peer-reviewed-documented route for thymalin, delivering the thymic polypeptide complex directly into systemic circulation for T-cell restoration and immune normalization. Protocols follow a discrete course-based structure — typically 5 to 10 consecutive daily injections repeated every 3 to 6 months — rather than continuous daily use, reflecting thymalin's mechanism of triggering a durable immune cascade that persists weeks after the course ends.
| Goal | Dosage | Frequency | Route |
|---|---|---|---|
| Restore T-cell counts and normalize thymic output in age-related or acquired immunodeficiency | 20 | 1 day range | SubQ or IM |
| Assess individual tolerability and baseline immune response with a reduced dose over 10 days | 5 | 1 day range | SubQ |
| Accelerate immune reconstitution and T-cell restoration following acute viral or bacterial illness | 10 | 1 day range | SubQ or IM |
| Counteract post-operative immune suppression and reduce infection risk during surgical recovery | 10 | 1 day range | SubQ or IM |
| Maintain baseline thymic activity and prevent immunosenescence through low-dose periodic courses | 5 | 1 day range | SubQ |
| Restore hematopoiesis and T-cell activity suppressed by cytotoxic chemotherapy or radiation | 10 | 1 day range | SubQ or IM |
Reconstitution Instructions
Materials needed:
Steps to reconstitute
- Allow the lyophilized thymalin vial to reach room temperature before reconstitution
- Draw 1–2 mL of bacteriostatic water into an insulin syringe
- Insert the needle through the vial stopper and inject the water slowly down the inside vial wall — never directly onto the powder
- Do not shake; gently swirl the vial until the powder fully dissolves — vigorous agitation degrades thymalin's short peptide chains
- Label the vial with the reconstitution date and resulting concentration (e.g., 10 mg/mL if 1 mL added to a 10 mg vial)
- Store reconstituted vial refrigerated at 2–8°C; use within 7–28 days depending on preparation sterility
- Draw the target dose volume into a fresh insulin syringe, removing any air bubbles
- Clean the injection site (lower abdomen, anterior thigh, or posterior upper arm) with an alcohol swab and allow to dry
- Pinch the skin, insert the needle at 45–90 degrees, and inject slowly over 5–10 seconds
- Withdraw the needle, apply gentle pressure with a clean swab, and dispose of the syringe in a sharps container
- Rotate injection sites with at least 1 inch spacing; do not reuse a site within 7 days
Thymalin Cycle
The Thymalin Cycle section explains how long a typical cycle lasts and what to expect during each phase. Over time, your body can become less responsive with continuous use.
Taking breaks between cycles may help maintain effectiveness and support better overall results.
- Day 1-5
- Initial immune modulation begins
- Day 5-10
- T-cell activation and differentiation enhanced
- Week 2-4
- Improved immune markers on blood tests
- Month 1-3
- Reduced illness frequency
- Week 0-0
- Geroprotective benefits with annual cycles
Dosing tools
ThymalinBlend Dosage Calculator
Calculate blended peptide doses with our visual syringe guide.
Volume of bacteriostatic water you add to reconstitute the powder. Use BAC water for preservation.
1 mL
2 mL
3 mL
5 mL
Custom
Blend Composition
Total: 0.0 mg
The compound you want to dose by. Results will be calculated based on this compound.
The dose of the anchor compound you want per administration, in mcg or mg.
Conversion: 1,000 mcg = 1 mg
Results
Concentration
—
Doses per vial
—
Total blend to inject
—
You'll get:
1mL / 100 units
< 1 units
0.000 mL
Dosing Guidance
Recommended Schedule
once daily
Supply Duration
This vial provides 0 doses (0-day supply at 250 mcg daily)
Peptide Interactions
Research suggestions of Thymalin interactions with other common peptides and substances.
Longevity
epi
Immunity
Epitalon
SYNERGISTIC
Combined use showed 4.1-fold mortality reduction vs 2.0-fold with Thymalin alone.
Longevity
vil
Immunity
Vilon
SYNERGISTIC
Often used together for enhanced systemic recovery and immune support.
Side effects
Contraindications
Autoimmune diseases (use with caution)
Organ transplant recipients (immunosuppression needed)
Pregnancy or breastfeeding
Known hypersensitivity
Stop signs
Signs of allergic reaction
Unusual immune responses
Bad signs
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Frequently asked questions
What is a peptide dosage calculator?
A peptide dosage calculator is a free tool that converts your vial size, bacteriostatic water volume, and target dose into an exact syringe draw volume. Instead of doing the reconstitution math by hand, you enter three inputs and instantly get the concentration of your solution and how many milliliters or syringeunits to draw. This calculator works for single peptide compounds and multi-peptide blends.
How do I calculate peptide dosage from a vial?
To calculate your peptide dose, divide the total peptide content of your vial in micrograms by the volume of bacteriostatic water you added in milliliters. This gives you your solution concentration in mcg/mL. Then divide your target dose by that concentration to get your draw volume. For example, a 5mg (5,000 mcg) vial reconstituted with 2mL of BAC water gives a concentration of 2,500 mcg/mL. A 250 mcg dose would require drawing 0.1mL. This calculator automates all of those steps instantly.
How much Bacteriostatic water should I add to a peptide vial?
Most people add 2mL to 3mL of bacteriostatic water per vial, but the right amount depends on the dose you want to draw and the syringe size you are using. Adding 1mL to a 5mg vial gives you a concentration of 5,000 mcg/mL, making each dose very small in volume. Adding 2mL gives you 2,500 mcg/mL, which is easier to measure on a standard insulin syringe. A general guideline is to choose a volume that puts your typical dose somewhere between 10 and 30 units on a U-100 syringe. Use the calculator above to test different water volumes and find what works for your dose.
How are peptides different from proteins?
Both are made of amino acids, but peptides are much smaller than proteins. Because of their tiny size, peptides can act like tiny messengers in the body, sending specific signals to your cells to tell them exactly what to do.
ThymalinResearch References
Thymalin is an approved compound
Thymalin
Thymalin is an approved compound
The Use of Thymalin for Immunocorrection and Molecular Aspects of Biological Activity
Detailed molecular mechanisms of Thymalin's immunoprotective action through peptides KE, EW, EDP binding to DNA and histones.
n.d.
Peptides of Pineal Gland and Thymus Prolong Human Life
6-8 year study showed 2.0-2.1 fold mortality reduction with Thymalin; 4.1-fold when combined with Epithalamin.
n.d.
Peptide Drug Thymalin Regulates Immune Status in Severe COVID-19 Older Patients
Faster lymphopenia reversal and improved recovery of CD4+, CD3+HLA-DR+, B-cells, and NK-cells in COVID-19.
n.d.
Effect of Thymalin on Tumor and Thymus in Activation Therapy
Antitumor effects with tumor growth arrest in >50% of animals; growth suppressed by 78% in others.
n.d.
Calculate peptide dosages
Learning how to calculate a peptide dose? Use our beginner-friendly peptide dosage, blend, and accumulation calculators. Enter vial size, reconstitution volume, and target dose to get exact draw volumes instantly. No guesswork, just clear guidance that helps prevent common mistakes.
