Cognitive
PE-22-28
Longevity
7
Amino acids
Molecular weight
Peptide
Type
Synthetic heptapeptide derived from Spadin positions 22-28, functioning as potent TREK-1 antagonist with enhanced selectivity and duration versus parent compound. Primary research focus on rapid antidepressant effects.
Top researched benefits
Overview of PE-22-28
Selectively blocks TREK-1 potassium channels (IC50: 0.12 nM). Enhances serotonin neurotransmission in dorsal raphe nucleus, triggering CREB activation and hippocampal neurogenesis.
Mental Health
- Primary research focus with rapid effects in behavioral models within 4 days.
- Anxiolytic properties demonstrated in preclinical anxiety models.
Neurogenesis
- Nearly doubles BrdU-positive cells after 4-day treatment.
- Promotes new synapse formation through CREB activation.
Cognition
- Hippocampal and prefrontal cortex TREK-1 expression supports memory.
- Potential ischemic protection and neuronal survival support.
Storage
Refrigerate at 2-8°C, use within 4-6 weeks
Frequency
Once daily
Typical Dose
50-200mcg daily
Cycle Duration
4-8 weeks
Chemical Makeup
Key benefits
Rapid antidepressant effects within 4 days (preclinical)
Hippocampal neurogenesis and synaptogenesis
Enhanced serotonergic neurotransmission
Extended ~23 hour duration of action
300-500x greater potency than full-length Spadin
Community interest
This peptide is still gaining traction in the community.
TREK-1 Channel Blocker | Shortened Spadin Analog
This overview is informational and based on aggregated descriptions from studies and user reports.
Was it helpful?YesPE-22-28 Molecular Information
View the scientifc details of PE-22-28.
7
Amino Acids
PE-22-28
Gly
Gly
Position 1
Val
Val
Position 2
Ser
Ser
Position 3
Trp
Trp
Position 4
Gly
Gly
Position 5
Leu
Leu
Position 6
Arg
Arg
Position 7
Molecular Weight
773.9DaChain Length
7Amino AcidsType
PeptidePE-22-28 Protocols
Subcutaneous injection to abdominal fat or thigh with site rotation.
| Goal | Dosage | Frequency | Route |
|---|---|---|---|
| Antidepressant Effect | 50 | 1 week range | SubQ |
| Neurogenesis Support | 100 | 1 week range | SubQ |
Reconstitution Instructions
Materials needed:
Steps to reconstitute
- Inject BAC water slowly down vial wall
- Gently swirl (do not shake)
- Store refrigerated 2-8°C
- Use within 4-6 weeks
PE-22-28 Cycle
The PE-22-28 Cycle section explains how long a typical cycle lasts and what to expect during each phase. Over time, your body can become less responsive with continuous use.
Taking breaks between cycles may help maintain effectiveness and support better overall results.
- Day 1-4
- Measurable antidepressant effects in preclinical models
- Week 1-2
- Neurogenesis and synaptogenesis processes established
- Week 2-4
- Potential mood and cognitive improvements emerge
- Week 4-8
- Sustained treatment allows full neurogenic effects
Dosing tools
PE-22-28 Peptide Dosage Calculator
Calculate peptide doses with our visual syringe guide.
0.3mL / 30 units
5 units
0.050 mL
1 mL
2 mL
3 mL
5 mL
Custom
Conversion: 1,000 mcg = 1 mg
Injection Results
Based on your vial and dilution inputs.
Safe concentration range
PE-22-28
SINGLE COMPOUNDVolume per injection
0.05
mL
Concentration
10.00
mg/mL
Doses per vial
20
doses
Total injections per vial
20 injections
How it works
Based on a 10 mg PE-22-28 vial diluted with 1 mL of bacteriostatic water, each 500 mcg injection equals 0.05 mL.
Research Purposes Only
These calculators are provided for educational and research purposes only. Always verify calculations and consult with qualified professionals. The information provided is not medical advice. Peptides should only be used in accordance with applicable laws and regulations.
Reference Guide
Dosing Cycle
- Peptide
- PE-22-28
- Dosing
- 50-200mcg daily
- Dosing Frequency
- Once daily
- Cycle Duration
- 4-8 weeks
- Storage
- Refrigerate at 2-8°C, use within 4-6 weeks
Note: Triple agonist; microdose for fewer side effects
Reconstitution Tips
- Use bacteriostatic water (BAC) — contains 0.9% benzyl alcohol for preservation
- Inject water slowly — aim down the vial wall, not directly onto powder
- Never shake — gently swirl or roll the vial until dissolved
- Store properly — refrigerate at 2-8°C after reconstitution
- Use within 28 days — most reconstituted peptides remain stable for about 4 weeks
- Keep sterile — always clean vial tops with alcohol before drawing
Peptide Interactions
Research suggestions of PE-22-28 interactions with other common peptides and substances.
Side effects
Contraindications
Pregnancy and breastfeeding
Concurrent MAOI use
Stop signs
Serotonin syndrome signs
Severe persistent headaches
Cardiac symptoms
Seizure activity
Severe mood changes or suicidal ideation
Bad signs
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Frequently asked questions
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Peptides can support cellular repair, immune function, metabolic health, and tissue regeneration. Research suggests they may help with recovery, sleep quality, skin health, and cognitive function, depending on the specific peptide and its mechanism of action.
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Current research explores peptides for longevity, muscle recovery, wound healing, metabolic disorders, and neuroprotection. Scientists are also investigating peptide-based drug delivery and targeted therapies that could offer more precise treatment options.
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Peptides work by binding to receptors on cells and triggering specific biological responses. Depending on the peptide, they may promote growth hormone release, support collagen production, modulate inflammation, or influence neurotransmitter activity—each with different implications for health and wellness.
Where can I find the latest studies on peptide applications?
Peer-reviewed journals such as Nature, Science, and specialized publications like Peptides and the Journal of Peptide Science publish ongoing research. PubMed and Google Scholar are useful for searching studies by peptide name or condition.
Are there any guidelines for using peptides in research?
Research use of peptides typically follows institutional review board (IRB) protocols and regulatory guidelines. Dosage, administration route, and safety monitoring should align with published literature and applicable regulations in your jurisdiction.
What is the difference between peptides and proteins?
Peptides are short chains of amino acids (typically under 50), while proteins are longer chains that fold into complex structures. Peptides are often more stable, easier to synthesize, and can cross cell membranes more readily, making them attractive for therapeutic applications.
How should peptides be stored?
Most peptides require refrigeration (2–8°C) and protection from light. Reconstituted peptides often have shorter stability and may need to be used within days or weeks. Always follow the manufacturer's or research protocol's storage instructions.
Can peptides be taken orally?
Some peptides are bioavailable orally, but many are broken down by digestive enzymes before reaching the bloodstream. Subcutaneous injection, nasal administration, or other routes are often used in research to improve bioavailability. The optimal route depends on the specific peptide.
PE-22-28Research References
PE-22-28 is an emerging compound
PE-22-28
PE-22-28 is an emerging compound
Shortened Spadin Analogs Display Better TREK-1 Inhibition
PE-22-28 IC50 0.12 nM vs 40-60 nM for Spadin; ~23 hour duration. Frontiers in Pharmacology.
2017
Spadin: A Sortilin-Derived Peptide Targeting TREK-1 Channels
Original discovery of TREK-1 blockade as antidepressant mechanism. PLOS Biology.
2010
TREK-1 Deletion Results in Depression-Resistant Phenotype
TREK-1 knockout mice show depression-resistant behavior in 5 tests. Nature Neuroscience.
2006