Cognitive
PE-22-28
Longevity
7
Amino acids
Molecular weight
Peptide
Type
Synthetic heptapeptide derived from Spadin positions 22-28, functioning as potent TREK-1 antagonist with enhanced selectivity and duration versus parent compound. Primary research focus on rapid antidepressant effects.
Top researched benefits
Overview of PE-22-28
Selectively blocks TREK-1 potassium channels (IC50: 0.12 nM). Enhances serotonin neurotransmission in dorsal raphe nucleus, triggering CREB activation and hippocampal neurogenesis.
mental health
- Primary research focus with rapid effects in behavioral models within 4 days.
- Anxiolytic properties demonstrated in preclinical anxiety models.
neurogenesis
- Nearly doubles BrdU-positive cells after 4-day treatment.
- Promotes new synapse formation through CREB activation.
cognition
- Hippocampal and prefrontal cortex TREK-1 expression supports memory.
- Potential ischemic protection and neuronal survival support.
Typical Dose
50-200mcg daily
Frequency
Once daily
Cycle Duration
4-8 weeks
Storage
Refrigerate at 2-8°C, use within 4-6 weeks
Chemical Makeup
Key benefits
Rapid antidepressant effects within 4 days (preclinical)
Hippocampal neurogenesis and synaptogenesis
Enhanced serotonergic neurotransmission
Extended ~23 hour duration of action
300-500x greater potency than full-length Spadin
Community interest
This peptide is still gaining traction in the community.
TREK-1 Channel Blocker | Shortened Spadin Analog
This overview is informational and based on aggregated descriptions from studies and user reports.
Was it helpful?YesNoPE-22-28 Molecular Information
View the scientifc details of PE-22-28.
7
Amino Acids
PE-22-28
Gly
Gly
Position 1
Val
Val
Position 2
Ser
Ser
Position 3
Trp
Trp
Position 4
Gly
Gly
Position 5
Leu
Leu
Position 6
Arg
Arg
Position 7
Molecular Weight
773.9DaChain Length
7Amino AcidsType
PeptidePE-22-28 Protocols
Subcutaneous injection to abdominal fat or thigh with site rotation.
| Goal | Dosage | Frequency | Route |
|---|---|---|---|
| Antidepressant Effect | 50 | 1 week range | SubQ |
| Neurogenesis Support | 100 | 1 week range | SubQ |
Reconstitution Instructions
Materials needed:
Steps to reconstitute
- Inject BAC water slowly down vial wall
- Gently swirl (do not shake)
- Store refrigerated 2-8°C
- Use within 4-6 weeks
PE-22-28 Cycle
The PE-22-28 Cycle section explains how long a typical cycle lasts and what to expect during each phase. Over time, your body can become less responsive with continuous use.
Taking breaks between cycles may help maintain effectiveness and support better overall results.
- Day 1-4
- Measurable antidepressant effects in preclinical models
- Week 1-2
- Neurogenesis and synaptogenesis processes established
- Week 2-4
- Potential mood and cognitive improvements emerge
- Week 4-8
- Sustained treatment allows full neurogenic effects
Dosing tools
PE-22-28 Peptide Dosage Calculator
Calculate peptide doses with our visual syringe guide.
mg
Enter the total amount of peptide in the vial in milligrams (as stated on the label).
The dose you want to inject per administration, in mcg or mg.
1,000 mcg = 1 mg
1 mL
2 mL
3 mL
5 mL
Custom
Volume of bacteriostatic water you add to reconstitute the powder. Use BAC water for preservation.
Injection Results
Based on your vial and dilution inputs.
PE-22-28
SINGLE COMPOUNDVolume per injection
0.05
mL
Concentration
10.00
mg/mL
Doses per vial
20
doses
Total injections per vial
20 injections
How it works
Based on a 10 mg PE-22-28 vial diluted with 1 mL of bacteriostatic water, each 500 mcg injection equals 0.05 mL.
1mL / 100 units
5 units
0.050 mL
Reference Guide
Dosing Cycle
- Peptide
- PE-22-28
- Dosing
- 50-200mcg daily
- Dosing Frequency
- Once daily
- Cycle Duration
- 4-8 weeks
- Storage
- Refrigerate at 2-8°C, use within 4-6 weeks
Note: Triple agonist; microdose for fewer side effects
Reconstitution Tips
- Use bacteriostatic water (BAC) — contains 0.9% benzyl alcohol for preservation
- Inject water slowly — aim down the vial wall, not directly onto powder
- Never shake — gently swirl or roll the vial until dissolved
- Store properly — refrigerate at 2-8°C after reconstitution
- Use within 28 days — most reconstituted peptides remain stable for about 4 weeks
- Keep sterile — always clean vial tops with alcohol before drawing
Peptide Interactions
Research suggestions of PE-22-28 interactions with other common peptides and substances.
Side effects
Contraindications
Pregnancy and breastfeeding
Concurrent MAOI use
Stop signs
Serotonin syndrome signs
Severe persistent headaches
Cardiac symptoms
Seizure activity
Severe mood changes or suicidal ideation
Bad signs
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Frequently asked questions
What is a peptide dosage calculator?
A peptide dosage calculator is a free tool that converts your vial size, bacteriostatic water volume, and target dose into an exact syringe draw volume. Instead of doing the reconstitution math by hand, you enter three inputs and instantly get the concentration of your solution and how many milliliters or syringeunits to draw. This calculator works for single peptide compounds and multi-peptide blends.
How do I calculate peptide dosage from a vial?
To calculate your peptide dose, divide the total peptide content of your vial in micrograms by the volume of bacteriostatic water you added in milliliters. This gives you your solution concentration in mcg/mL. Then divide your target dose by that concentration to get your draw volume. For example, a 5mg (5,000 mcg) vial reconstituted with 2mL of BAC water gives a concentration of 2,500 mcg/mL. A 250 mcg dose would require drawing 0.1mL. This calculator automates all of those steps instantly.
How much Bacteriostatic water should I add to a peptide vial?
Most people add 2mL to 3mL of bacteriostatic water per vial, but the right amount depends on the dose you want to draw and the syringe size you are using. Adding 1mL to a 5mg vial gives you a concentration of 5,000 mcg/mL, making each dose very small in volume. Adding 2mL gives you 2,500 mcg/mL, which is easier to measure on a standard insulin syringe. A general guideline is to choose a volume that puts your typical dose somewhere between 10 and 30 units on a U-100 syringe. Use the calculator above to test different water volumes and find what works for your dose.
How are peptides different from proteins?
Both are made of amino acids, but peptides are much smaller than proteins. Because of their tiny size, peptides can act like tiny messengers in the body, sending specific signals to your cells to tell them exactly what to do.
PE-22-28Research References
PE-22-28 is an emerging compound
PE-22-28
PE-22-28 is an emerging compound
Shortened Spadin Analogs Display Better TREK-1 Inhibition
PE-22-28 IC50 0.12 nM vs 40-60 nM for Spadin; ~23 hour duration. Frontiers in Pharmacology.
2017
Spadin: A Sortilin-Derived Peptide Targeting TREK-1 Channels
Original discovery of TREK-1 blockade as antidepressant mechanism. PLOS Biology.
2010
TREK-1 Deletion Results in Depression-Resistant Phenotype
TREK-1 knockout mice show depression-resistant behavior in 5 tests. Nature Neuroscience.
2006