Cognitive
DSIP
Sleep
9
Amino acids
Molecular weight
Peptide
Type
DSIP (Delta Sleep-Inducing Peptide) is a naturally occurring nonapeptide (sequence: Trp-Ala-Gly-Gly-Asp-Ala-Ser-Gly-Glu) first isolated from rabbit cerebral venous blood during slow-wave sleep induction. It promotes delta-wave (slow-wave) sleep, modulates cortisol and stress hormone levels, and has demonstrated analgesic and anticonvulsant properties in preclinical models. Used primarily in research contexts exploring sleep architecture, neuroendocrine regulation, and neuroprotection.
Top researched benefits
Overview of DSIP
DSIP is believed to interact with GABA-A, glycine, and NMDA receptor subunits to modulate inhibitory neurotransmission and promote slow-wave sleep. It also engages components of the MAPK signaling cascade and shares homology with glucocorticoid-induced leucine zipper (GILZ), suggesting a role in glucocorticoid-mediated stress regulation.
sleep
- Promotes delta wave sleep, the most restorative sleep phase for physical recovery.
- Reduces sleep latency and nocturnal awakenings without morning grogginess.
- Supports natural sleep cycles rather than forcing sedation like traditional sleep aids.
mood
- Helps regulate cortisol and stress response through HPA axis modulation.
- Supports emotional balance without sedation.
- May help reduce anxiety through neuromodulatory effects.
pain and recovery
- Significantly reduced pain levels in 6 of 7 patients with chronic pain conditions.
- Helps manage withdrawal symptoms in alcohol and opioid-dependent patients.
- Supports post-training recovery through enhanced sleep quality.
Typical Dose
100–250mcg per injection
Frequency
once daily, 30–60 minutes before bed
Cycle Duration
4 weeks on, 4 weeks off
Storage
Lyophilized: store at -20°C, protected from light and moisture, stable up to 36 months. Reconstituted: 2–8°C for up to 21–28 days; for long-term storage, freeze below -18°C.
Chemical Makeup
Key benefits
Promotes slow-wave (delta) sleep by modulating inhibitory receptor activity, increasing restorative deep sleep duration without sedative-like suppression of REM
Reduces elevated cortisol and normalizes disrupted sleep patterns, improving stress tolerance and daytime alertness in double-blind human studies
Demonstrates antinociceptive (pain-reducing) effects when administered centrally in animal models, suggesting potential as an analgesic adjunct
Acts as an anticonvulsant in rat models of metaphit-induced epilepsy, significantly reducing incidence and duration of seizures
Community interest
This peptide is still gaining traction in the community.
Sleep Neuropeptide | Sleep Quality & Stress Modulation
This overview is informational and based on aggregated descriptions from studies and user reports.
Was it helpful?YesNoDSIP Molecular Information
View the scientifc details of DSIP.
9
Amino Acids
DSIP
Trp
Trp
Position 1
Ala
Ala
Position 2
Gly
Gly
Position 3
Gly
Gly
Position 4
Asp
Asp
Position 5
Ala
Ala
Position 6
Ser
Ser
Position 7
Gly
Gly
Position 8
Glu
Glu
Position 9
Molecular Weight
848.82DaChain Length
9Amino AcidsType
PeptideDSIP Protocols
Subcutaneous injection is the primary and most researched route for DSIP, with administration timed 1–3 hours before sleep to align with the peptide's sleep-promoting effects. The abdominal area 2+ inches from the navel is the preferred injection site, with regular rotation to thigh and upper arm.
| Goal | Dosage | Frequency | Route |
|---|---|---|---|
| Improve sleep onset latency and slow-wave sleep depth on a nightly maintenance schedule | 250 | 1 day range | SubQ |
| Establish individual sensitivity at a minimal daily dose to rule out paradoxical insomnia before escalating | 100 | 1 day range | SubQ |
| Attenuate HPA axis overactivation and reduce stress-driven sleep disruption at a conservative nightly dose | 100 | 1 day range | SubQ |
| Accelerate soft-tissue repair and growth hormone secretion during post-training deep sleep by dosing only on hard training days | 250 | 3 week range | SubQ |
| Prevent tolerance buildup and maintain receptor sensitivity with a fixed 10-night-on, 20-night-off cycle | 200 | 1 day range | SubQ |
| Reduce opiate or alcohol withdrawal-related sleep disruption and autonomic dysregulation under clinical supervision | 25 | 1 day range | SubQ or IV (clinical setting) |
Reconstitution Instructions
Materials needed:
Steps to reconstitute
- Remove the vial from the freezer and allow it to reach room temperature before handling.
- Wipe the vial septum with a 70% isopropyl alcohol swab and allow it to dry.
- Draw bacteriostatic water into a sterile syringe — 2 mL for a 5 mg vial (yields 2,500 mcg/mL) or 3 mL for a 10 mg vial (yields 3,333 mcg/mL).
- Inject the bacteriostatic water slowly down the inside wall of the vial — never directly onto the powder.
- Gently swirl the vial until the powder is fully dissolved — do not shake or vortex.
- Store reconstituted vial refrigerated at 2–8°C; use within 28 days. Do not freeze the reconstituted solution.
- Wipe the injection site with 70% isopropyl alcohol and allow to dry.
- Pinch a fold of skin at the injection site (abdomen, thigh, or upper arm), insert the insulin syringe at a 45-degree angle, and inject slowly.
- Rotate injection sites with each administration to prevent lipohypertrophy.
DSIP Cycle
The DSIP Cycle section explains how long a typical cycle lasts and what to expect during each phase. Over time, your body can become less responsive with continuous use.
Taking breaks between cycles may help maintain effectiveness and support better overall results.
- Week 0-0
- Noticeable sleep pressure and easier sleep onset
- Week 0-0
- Deeper sleep, fewer awakenings, vivid dreams possible
- Day 2-5
- Improved morning refreshment, stable daytime energy
- Day 5-10
- Cumulative stress reduction, mood improvements
- Week 0-0
- Benefits may persist 1-2 weeks after stopping
Dosing tools
DSIP Peptide Dosage Calculator
Calculate peptide doses with our visual syringe guide.
mg
Enter the total amount of peptide in the vial in milligrams (as stated on the label).
The dose you want to inject per administration, in mcg or mg.
1,000 mcg = 1 mg
1 mL
2 mL
3 mL
5 mL
Custom
Volume of bacteriostatic water you add to reconstitute the powder. Use BAC water for preservation.
Injection Results
Based on your vial and dilution inputs.
DSIP
SINGLE COMPOUNDVolume per injection
0.05
mL
Concentration
10.00
mg/mL
Doses per vial
20
doses
Total injections per vial
20 injections
How it works
Based on a 10 mg DSIP vial diluted with 1 mL of bacteriostatic water, each 500 mcg injection equals 0.05 mL.
1mL / 100 units
5 units
0.050 mL
Reference Guide
Dosing Cycle
- Peptide
- DSIP
- Dosing
- 100–250mcg per injection
- Dosing Frequency
- once daily, 30–60 minutes before bed
- Cycle Duration
- 4 weeks on, 4 weeks off
- Storage
- Lyophilized: store at -20°C, protected from light and moisture, stable up to 36 months. Reconstituted: 2–8°C for up to 21–28 days; for long-term storage, freeze below -18°C.
Note: Triple agonist; microdose for fewer side effects
Reconstitution Tips
- Use bacteriostatic water (BAC) — contains 0.9% benzyl alcohol for preservation
- Inject water slowly — aim down the vial wall, not directly onto powder
- Never shake — gently swirl or roll the vial until dissolved
- Store properly — refrigerate at 2-8°C after reconstitution
- Use within 28 days — most reconstituted peptides remain stable for about 4 weeks
- Keep sterile — always clean vial tops with alcohol before drawing
Peptide Interactions
Research suggestions of DSIP interactions with other common peptides and substances.
Longevity
epi
Immunity
Epitalon
SYNERGISTIC
Both support circadian regulation and sleep quality.
Side effects
Contraindications
Avoid driving until effects are known
Pregnancy or breastfeeding
Stop signs
Excessive daytime sedation
Paradoxical insomnia or agitation
Persistent headaches
Any allergic reactions
Mood changes or depression
Unusual dreams causing distress
Bad signs
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Frequently asked questions
What is a peptide dosage calculator?
A peptide dosage calculator is a free tool that converts your vial size, bacteriostatic water volume, and target dose into an exact syringe draw volume. Instead of doing the reconstitution math by hand, you enter three inputs and instantly get the concentration of your solution and how many milliliters or syringeunits to draw. This calculator works for single peptide compounds and multi-peptide blends.
How do I calculate peptide dosage from a vial?
To calculate your peptide dose, divide the total peptide content of your vial in micrograms by the volume of bacteriostatic water you added in milliliters. This gives you your solution concentration in mcg/mL. Then divide your target dose by that concentration to get your draw volume. For example, a 5mg (5,000 mcg) vial reconstituted with 2mL of BAC water gives a concentration of 2,500 mcg/mL. A 250 mcg dose would require drawing 0.1mL. This calculator automates all of those steps instantly.
How much Bacteriostatic water should I add to a peptide vial?
Most people add 2mL to 3mL of bacteriostatic water per vial, but the right amount depends on the dose you want to draw and the syringe size you are using. Adding 1mL to a 5mg vial gives you a concentration of 5,000 mcg/mL, making each dose very small in volume. Adding 2mL gives you 2,500 mcg/mL, which is easier to measure on a standard insulin syringe. A general guideline is to choose a volume that puts your typical dose somewhere between 10 and 30 units on a U-100 syringe. Use the calculator above to test different water volumes and find what works for your dose.
How are peptides different from proteins?
Both are made of amino acids, but peptides are much smaller than proteins. Because of their tiny size, peptides can act like tiny messengers in the body, sending specific signals to your cells to tell them exactly what to do.
DSIPResearch References
DSIP is a preclinical compound
DSIP
DSIP is a preclinical compound
Chronic Insomnia Double-Blind Study
15-30 nmol for 6 consecutive nights improved objective sleep quality with higher sleep efficiency and shorter sleep latency compared to placebo.
1991
Chronic Pain Pilot Study
25 nmol IV for 10 days significantly reduced pain levels in 6 of 7 patients with chronic pain conditions including migraine and tinnitus.
1984
Alcohol and Opioid Withdrawal Study
DSIP administration helped manage withdrawal symptoms in both alcohol and opioid-dependent patients over 5-10 days.
1984
Sleep Pressure Induction Study
Single 25 nmol/kg IV dose showed 59% increase in total sleep time within 130 minutes; improved subsequent night sleep efficiency.
1983
Stroke Recovery in Rats
120mcg/kg nasal for 7 days enhanced motor recovery without significantly reducing infarct size.
2021
Calculate peptide dosages
Learning how to calculate a peptide dose? Use our beginner-friendly peptide dosage, blend, and accumulation calculators. Enter vial size, reconstitution volume, and target dose to get exact draw volumes instantly. No guesswork, just clear guidance that helps prevent common mistakes.
