Healing
BPC-157
Longevity
15
Amino acids
Molecular weight
Peptide
Type
BPC-157 (Body Protection Compound-157) is a synthetic 15-amino acid pentadecapeptide derived from a protective protein found in human gastric juice. It promotes tissue repair and regeneration by stimulating angiogenesis, upregulating growth factor signaling, and modulating nitric oxide production. It is widely used in preclinical research and by athletes and biohackers for accelerating healing of musculoskeletal injuries and gut conditions.
Top researched benefits
Overview of BPC-157
BPC-157 activates VEGFR2 and the Akt-eNOS signaling axis to stimulate nitric oxide synthesis and angiogenesis, while engaging ERK1/2 pathways to promote fibroblast proliferation and endothelial cell migration. It also upregulates growth hormone receptor expression in tendon fibroblasts, enhancing tissue responsiveness to circulating GH.
wound healing
- Accelerated tendon-to-bone healing with improved biomechanical properties; most effective with localized injection.
- Enhanced healing and reduced recovery following crush injuries and surgical procedures with direct tissue targeting.
- Promotes blood vessel formation and improves vascularization through localized delivery.
neurological
- Protective effects against neurotoxic agents and ischemic brain injury models.
- Improved functional recovery and reduced tissue damage in spinal cord injury studies.
- Enhanced peripheral nerve regeneration and functional recovery after injury.
gastrointestinal
- Protective effects against gastric and duodenal ulcers through cytoprotective mechanisms.
- Acceleration of intestinal healing with reduced inflammatory markers in IBD models.
- Enhanced mucosal barrier function and accelerated epithelial regeneration.
Typical Dose
250–500mcg per injection
Frequency
once or twice daily
Cycle Duration
4–8 weeks, followed by a break
Storage
Lyophilized: store at -20°C, stable up to 24–36 months. Reconstituted with bacteriostatic water: refrigerate at 2–8°C for up to 4 weeks. Protect from repeated freeze-thaw cycles.
Chemical Makeup
Key benefits
Accelerates tendon and ligament healing by upregulating VEGF and growth factor signaling at the injury site
Promotes gut mucosal repair and reduces intestinal inflammation, beneficial for conditions like ulcers and IBD
Stimulates angiogenesis to restore blood flow and accelerate tissue regeneration following muscle injuries
Enhances neuroprotection by reducing oxidative stress and edema in models of traumatic brain injury
Community interest
This peptide is still gaining traction in the community.
Gastric Pentadecapeptide | Tissue Repair & Recovery
This overview is informational and based on aggregated descriptions from studies and user reports.
Was it helpful?YesNoBPC-157 Molecular Information
View the scientifc details of BPC-157.
15
Amino Acids
BPC-157
Gly
Gly
Position 1
Glu
Glu
Position 2
Pro
Pro
Position 3
Pro
Pro
Position 4
Pro
Pro
Position 5
Gly
Gly
Position 6
Lys
Lys
Position 7
Pro
Pro
Position 8
Ala
Ala
Position 9
Asp
Asp
Position 10
Asp
Asp
Position 11
Ala
Ala
Position 12
Gly
Gly
Position 13
Leu
Leu
Position 14
Val
Val
Position 15
Molecular Weight
1419.54DaChain Length
15Amino AcidsType
PeptideBPC-157 Protocols
Subcutaneous injection near the injury site is the primary route for BPC-157, offering approximately 90% bioavailability and direct localized delivery for tendon, ligament, and muscle healing. Intramuscular injection is an alternative for deep muscle injuries with faster absorption (10–20 minutes vs. 30–60 minutes for SubQ).
| Goal | Dosage | Frequency | Route |
|---|---|---|---|
| Reduce tendon inflammation and stimulate collagen remodeling via VEGFR2-driven angiogenesis in mildly damaged tendon tissue | 250 | 1 day range | SubQ near injury |
| Accelerate fibroblast proliferation and extracellular matrix repair in a partial ligament or tendon tear | 500 | 1 day range | SubQ near injury |
| Maximize tissue regeneration and reduce post-surgical inflammation across a full tear or surgical repair site | 500 | 2 day range | SubQ near injury |
| Reduce systemic inflammation and support whole-body tissue repair without a single localized injury target | 250 | 1 day range | SubQ abdomen |
| Deliver BPC-157 directly into a deep muscle belly to accelerate healing of a strain or tear; 250–500 mcg is the weight-based range reported in practice; 400 mcg falls within the 300–400 mcg range cited for 150–200 lb individuals but is not confirmed by a controlled human trial | 400 | 1 day range | IM into affected muscle |
| Support neuronal survival and reduce neuroinflammation following traumatic brain injury via FAK-paxillin and eNOS pathway activation | 300 | 1 day range | SubQ |
Reconstitution Instructions
Materials needed:
Steps to reconstitute
- Remove the plastic flip-cap from the BPC-157 vial and swab the rubber stopper with an alcohol pad; allow to dry for 10 seconds
- Draw 2 mL of bacteriostatic water (BAC water) into a 3 mL lab syringe (3 mL 3cc Disposable Plastic Syringe)
- Insert the needle into the vial at an angle, aiming the tip toward the inner glass wall — not directly at the powder
- Depress the plunger slowly so the BAC water runs down the vial wall; this prevents direct impact on the peptide powder
- Remove the syringe and gently swirl the vial in slow circular motions until the powder is fully dissolved (30–60 seconds) — never shake the vial
- For a 5 mg vial reconstituted with 2 mL of bacteriostatic water, the resulting solution is 2,500 mcg/mL; for a 250 mcg dose draw 0.1 mL (10 units on an insulin syringe), for 500 mcg draw 0.2 mL (20 units)
- Label the vial with the reconstitution date and concentration; refrigerate immediately at 2–8°C (36–46°F)
- For subcutaneous injection, clean the chosen site with an alcohol swab and allow to dry
- Each time you dose from the reconstituted vial, swab the rubber stopper with a fresh alcohol pad and allow to dry for 10 seconds
- Take a new insulin syringe (never reuse); insert the needle into the vial
- Draw your required dose into the insulin syringe — for a 5 mg vial reconstituted with 2 mL of bacteriostatic water, draw to 10 units for a 250 mcg dose
- Pinch a 1–2 inch skin fold at the injection site (abdomen 2 inches from navel, or close to the injury site) and insert the needle at a 45-degree angle
- Depress the plunger slowly over 3–5 seconds; withdraw the needle and apply gentle pressure with a clean pad — do not rub
- Rotate injection sites daily, moving at least 1–2 cm from the previous spot within the target area
- Discard any unused reconstituted solution after 30 days; do not freeze the reconstituted vial
BPC-157 Cycle
The BPC-157 Cycle section explains how long a typical cycle lasts and what to expect during each phase. Over time, your body can become less responsive with continuous use.
Taking breaks between cycles may help maintain effectiveness and support better overall results.
- Week 1-2
- Reduced inflammation and pain at injection site
- Week 2-4
- Improved healing rate and tissue repair
- Week 4-8
- Maximum localized healing benefits
Dosing tools
BPC-157 Peptide Dosage Calculator
Calculate peptide doses with our visual syringe guide.
mg
Enter the total amount of peptide in the vial in milligrams (as stated on the label).
The dose you want to inject per administration, in mcg or mg.
1,000 mcg = 1 mg
1 mL
2 mL
3 mL
5 mL
Custom
Volume of bacteriostatic water you add to reconstitute the powder. Use BAC water for preservation.
Injection Results
Based on your vial and dilution inputs.
BPC-157
SINGLE COMPOUNDVolume per injection
0.05
mL
Concentration
10.00
mg/mL
Doses per vial
20
doses
Total injections per vial
20 injections
How it works
Based on a 10 mg BPC-157 vial diluted with 1 mL of bacteriostatic water, each 500 mcg injection equals 0.05 mL.
1mL / 100 units
5 units
0.050 mL
Reference Guide
Dosing Cycle
- Peptide
- BPC-157
- Dosing
- 250–500mcg per injection
- Dosing Frequency
- once or twice daily
- Cycle Duration
- 4–8 weeks, followed by a break
- Storage
- Lyophilized: store at -20°C, stable up to 24–36 months. Reconstituted with bacteriostatic water: refrigerate at 2–8°C for up to 4 weeks. Protect from repeated freeze-thaw cycles.
Note: Triple agonist; microdose for fewer side effects
Reconstitution Tips
- Use bacteriostatic water (BAC) — contains 0.9% benzyl alcohol for preservation
- Inject water slowly — aim down the vial wall, not directly onto powder
- Never shake — gently swirl or roll the vial until dissolved
- Store properly — refrigerate at 2-8°C after reconstitution
- Use within 28 days — most reconstituted peptides remain stable for about 4 weeks
- Keep sterile — always clean vial tops with alcohol before drawing
Peptide Interactions
Research suggestions of BPC-157 interactions with other common peptides and substances.
Weight
aod
Metabolic
AOD-9604
COMPATIBLE
Safe combination; different pathways. Often combined in regenerative protocols.
Weight
ipa
Growth
Ipamorelin
SYNERGISTIC
BPC-157 increases GH receptor expression, amplifying growth hormone benefits.
Skin
mela
Sexual
Melanotan II
COMPATIBLE
No known interactions; different mechanisms and receptor targets.
Healing
tb5
Longevity
TB-500
SYNERGISTIC
Complementary healing mechanisms; often used together in the Wolverine Stack.
Side effects
Contraindications
Active cancer (due to angiogenic effects)
Pregnancy or breastfeeding
Blood thinners (consult doctor due to angiogenesis)
WADA prohibited for competitive athletes
Stop signs
Persistent injection site reactions or infection signs
Unusual swelling or rash around injection area
Severe headaches or dizziness
Allergic reactions
Persistent or worsening digestive issues
Bad signs
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Frequently asked questions
What is a peptide dosage calculator?
A peptide dosage calculator is a free tool that converts your vial size, bacteriostatic water volume, and target dose into an exact syringe draw volume. Instead of doing the reconstitution math by hand, you enter three inputs and instantly get the concentration of your solution and how many milliliters or syringeunits to draw. This calculator works for single peptide compounds and multi-peptide blends.
How do I calculate peptide dosage from a vial?
To calculate your peptide dose, divide the total peptide content of your vial in micrograms by the volume of bacteriostatic water you added in milliliters. This gives you your solution concentration in mcg/mL. Then divide your target dose by that concentration to get your draw volume. For example, a 5mg (5,000 mcg) vial reconstituted with 2mL of BAC water gives a concentration of 2,500 mcg/mL. A 250 mcg dose would require drawing 0.1mL. This calculator automates all of those steps instantly.
How much Bacteriostatic water should I add to a peptide vial?
Most people add 2mL to 3mL of bacteriostatic water per vial, but the right amount depends on the dose you want to draw and the syringe size you are using. Adding 1mL to a 5mg vial gives you a concentration of 5,000 mcg/mL, making each dose very small in volume. Adding 2mL gives you 2,500 mcg/mL, which is easier to measure on a standard insulin syringe. A general guideline is to choose a volume that puts your typical dose somewhere between 10 and 30 units on a U-100 syringe. Use the calculator above to test different water volumes and find what works for your dose.
How are peptides different from proteins?
Both are made of amino acids, but peptides are much smaller than proteins. Because of their tiny size, peptides can act like tiny messengers in the body, sending specific signals to your cells to tell them exactly what to do.
BPC-157Research References
BPC-157 is a preclinical compound
BPC-157
BPC-157 is a preclinical compound
Muscle Healing Research
Accelerated quadriceps healing with improved histology, reduced inflammation, and restored function in rat models.
2006
Spinal Cord Injury Recovery
Significant functional recovery, reduced tissue damage, and improved neurological outcomes in rat models.
2019
Gastric Ulcer Protection
Cytoprotective effects against gastric ulcers through multiple protective mechanisms.
2020
VEGF and Angiogenesis Study
Pro-angiogenic effects via VEGFR2 activation, explaining wound healing mechanisms.
2017
Safety Evaluation Study
No serious adverse effects at therapeutic doses up to 1000 μg/kg for 90 days; established safety profile.
2020
Achilles Tendon Healing Study
Superior tendon healing with improved biomechanical properties and enhanced tendon-to-bone integration in rat models.
2003
Calculate peptide dosages
Learning how to calculate a peptide dose? Use our beginner-friendly peptide dosage, blend, and accumulation calculators. Enter vial size, reconstitution volume, and target dose to get exact draw volumes instantly. No guesswork, just clear guidance that helps prevent common mistakes.
