Longevity
Livagen
Immunity
4
Amino acids
Molecular weight
Peptide
Type
Livagen is a synthetic tetrapeptide bioregulator (Lys-Glu-Asp-Ala) derived from liver tissue research that works by decondensing heterochromatin in aging cells, restoring transcription of previously silenced genes. It has been studied primarily in cell culture and animal models for its geroprotective effects on liver function, immune cells, and chromatin structure. Researchers and longevity-focused users explore it for hepatic regeneration and age-related gene expression restoration.
Top researched benefits
Overview of Livagen
Livagen promotes de-heterochromatinization — reversing age-related chromatin compaction in lymphocytes and hepatocytes — which reactivates ribosomal gene transcription suppressed by aging-induced condensation. It also inhibits enkephalin-degrading enzymes in human serum, preserving endogenous opioid peptides and modulating neuroendocrine signaling.
liver support
- Protects liver tissue through normalizing immune and antioxidant status.
- Restores liver functions in both acute and chronic hepatitis models.
- Shows protective properties in liver fibroid induration models.
antiaging
- Decondenses chromatin to restore youthful gene expression patterns.
- Maximum hepatoprotective effects occur during aging.
- Activates silent genes including ribosomal genes for improved protein synthesis.
immune support
- Normalizes immune function alongside liver protective effects.
Typical Dose
5–10mg per injection
Frequency
once daily
Cycle Duration
10-day course, repeated every 3–6 months
Storage
Lyophilized: -20°C, protected from light. Reconstituted in bacteriostatic water: 2–8°C for up to 28 days.
Chemical Makeup
Key benefits
Reverses age-related heterochromatin condensation in elderly lymphocytes, reactivating silenced ribosomal genes and restoring protein synthesis capacity
Increases hepatocyte protein synthesis in aged rat liver cell cultures, supporting liver metabolic enzyme production and detoxification gene expression
Inhibits enkephalin-degrading enzymes in human serum more potently than established inhibitors such as puromycin and leupeptin, preserving endogenous opioid activity
Decondenses chromatin in immune cells from elderly donors, potentially restoring immune gene expression that declines with age
Community interest
This peptide is still gaining traction in the community.
Chromatin Remodeler | Anti-Aging & Liver Support
This overview is informational and based on aggregated descriptions from studies and user reports.
Was it helpful?YesNoLivagen Molecular Information
View the scientifc details of Livagen.
4
Amino Acids
Livagen
Lys
Lys
Position 1
Glu
Glu
Position 2
Asp
Asp
Position 3
Ala
Ala
Position 4
Molecular Weight
461.46DaChain Length
4Amino AcidsType
PeptideLivagen Protocols
Subcutaneous or intramuscular injection is the primary delivery method for Livagen, a tetrapeptide bioregulator targeting liver function and epigenetic chromatin remodeling in aging cells. SubQ injection into abdominal tissue allows self-administration, while IM injection is used in Khavinson laboratory protocols for sustained systemic delivery.
| Goal | Dosage | Frequency | Route |
|---|---|---|---|
| Hepatoprotection and initial tolerance assessment over 5–7 days before advancing to standard dosing | 2 | 1 day range | SubQ |
| Restore hepatic chromatin activity and support liver cytoprotection across a 10-day Khavinson-style course | 5 | 1 day range | SubQ or IM |
| Induce chromatin decondensation in aged lymphocytes and restore ribosomal gene expression per Khavinson laboratory protocol | 10 | 1 day range | IM |
| Address significant age-related hepatic and immune function decline with an extended 10–14 day intensive course | 10 | 1 day range | IM |
| Support protein synthesis recovery and immune competence through pulsatile 20-day cycles repeated 2–3 times yearly | 5 | 1 day range | SubQ or IM |
Reconstitution Instructions
Materials needed:
Steps to reconstitute
- Allow the Livagen vial (lyophilized powder) to come to room temperature before reconstitution
- Draw 2–3 mL of bacteriostatic water into a syringe — this yields a concentration of approximately 6.7–10 mg/mL for a 20 mg vial
- Insert the needle into the vial at an angle and inject the bacteriostatic water slowly down the inside wall — do not aim the stream directly onto the powder
- Gently swirl the vial in a slow circular motion until the powder is fully dissolved — do not shake, as mechanical agitation degrades the peptide
- Inspect the solution — it should be clear and colorless; discard if cloudy or if particles are visible
- Label the vial with the reconstitution date and concentration, then store in the refrigerator at 2–8°C and use within 14–28 days
- Before each injection, wipe the vial septum with a fresh alcohol swab and draw the calculated dose into an insulin syringe
- For SubQ injection, pinch a fold of skin at the abdomen (2 inches from navel), outer thigh, or upper arm, insert the needle at 45–90°, inject slowly over 5–10 seconds, and withdraw
- For IM injection, use the vastus lateralis (outer thigh) or deltoid muscle; insert the needle perpendicular to the skin and inject slowly
- Rotate injection sites with each administration to prevent local tissue reactions and lipohypertrophy
Livagen Cycle
The Livagen Cycle section explains how long a typical cycle lasts and what to expect during each phase. Over time, your body can become less responsive with continuous use.
Taking breaks between cycles may help maintain effectiveness and support better overall results.
- Week 0-0
- Chromatin remodeling and gene expression changes begin
- Week 0-0
- Effects persist due to epigenetic changes
- Week 0-0
- Liver function improvements
- Week 0-0
- Cumulative benefits with periodic cycles
Dosing tools
Livagen Peptide Dosage Calculator
Calculate peptide doses with our visual syringe guide.
mg
Enter the total amount of peptide in the vial in milligrams (as stated on the label).
The dose you want to inject per administration, in mcg or mg.
1,000 mcg = 1 mg
1 mL
2 mL
3 mL
5 mL
Custom
Volume of bacteriostatic water you add to reconstitute the powder. Use BAC water for preservation.
Injection Results
Based on your vial and dilution inputs.
Livagen
SINGLE COMPOUNDVolume per injection
0.05
mL
Concentration
10.00
mg/mL
Doses per vial
20
doses
Total injections per vial
20 injections
How it works
Based on a 10 mg Livagen vial diluted with 1 mL of bacteriostatic water, each 500 mcg injection equals 0.05 mL.
1mL / 100 units
5 units
0.050 mL
Reference Guide
Dosing Cycle
- Peptide
- Livagen
- Dosing
- 5–10mg per injection
- Dosing Frequency
- once daily
- Cycle Duration
- 10-day course, repeated every 3–6 months
- Storage
- Lyophilized: -20°C, protected from light. Reconstituted in bacteriostatic water: 2–8°C for up to 28 days.
Note: Triple agonist; microdose for fewer side effects
Reconstitution Tips
- Use bacteriostatic water (BAC) — contains 0.9% benzyl alcohol for preservation
- Inject water slowly — aim down the vial wall, not directly onto powder
- Never shake — gently swirl or roll the vial until dissolved
- Store properly — refrigerate at 2-8°C after reconstitution
- Use within 28 days — most reconstituted peptides remain stable for about 4 weeks
- Keep sterile — always clean vial tops with alcohol before drawing
Peptide Interactions
Research suggestions of Livagen interactions with other common peptides and substances.
Longevity
epi
Immunity
Epitalon
SYNERGISTIC
Structurally similar; often combined in comprehensive anti-aging protocols.
Longevity
glu
Immunity
Glutathione
SYNERGISTIC
Both support liver function and antioxidant status.
Longevity
vil
Immunity
Vilon
COMPATIBLE
Different organ targets; can be combined in bioregulator protocols.
Side effects
Contraindications
Active liver emergencies (seek medical care)
Known hypersensitivity
Pregnancy or breastfeeding
Stop signs
Allergic reactions
Unusual liver symptoms
Bad signs
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Frequently asked questions
What is a peptide dosage calculator?
A peptide dosage calculator is a free tool that converts your vial size, bacteriostatic water volume, and target dose into an exact syringe draw volume. Instead of doing the reconstitution math by hand, you enter three inputs and instantly get the concentration of your solution and how many milliliters or syringeunits to draw. This calculator works for single peptide compounds and multi-peptide blends.
How do I calculate peptide dosage from a vial?
To calculate your peptide dose, divide the total peptide content of your vial in micrograms by the volume of bacteriostatic water you added in milliliters. This gives you your solution concentration in mcg/mL. Then divide your target dose by that concentration to get your draw volume. For example, a 5mg (5,000 mcg) vial reconstituted with 2mL of BAC water gives a concentration of 2,500 mcg/mL. A 250 mcg dose would require drawing 0.1mL. This calculator automates all of those steps instantly.
How much Bacteriostatic water should I add to a peptide vial?
Most people add 2mL to 3mL of bacteriostatic water per vial, but the right amount depends on the dose you want to draw and the syringe size you are using. Adding 1mL to a 5mg vial gives you a concentration of 5,000 mcg/mL, making each dose very small in volume. Adding 2mL gives you 2,500 mcg/mL, which is easier to measure on a standard insulin syringe. A general guideline is to choose a volume that puts your typical dose somewhere between 10 and 30 units on a U-100 syringe. Use the calculator above to test different water volumes and find what works for your dose.
How are peptides different from proteins?
Both are made of amino acids, but peptides are much smaller than proteins. Because of their tiny size, peptides can act like tiny messengers in the body, sending specific signals to your cells to tell them exactly what to do.
LivagenResearch References
Livagen is a preclinical compound
Livagen
Livagen is a preclinical compound
Influence of KEDA Tetrapeptide on Organism Physiological Function
KEDA exhibits hepatoprotective, immunomodulatory, and geroprotective properties with maximum effects during aging.
n.d.
Effects of Livagen Peptide on Chromatin Activation in Lymphocytes from Old People
Livagen activates chromatin decondensation and multiple genes in aged lymphocytes.
n.d.
Hepatoprotective Properties of KEDA in Hepatitis Models
KEDA normalizes immune and antioxidant status and restores liver functions in hepatitis.
n.d.
Khavinson Peptide Bioregulators
Comprehensive review of bioregulator peptides and their epigenetic mechanisms.
n.d.
Calculate peptide dosages
Learning how to calculate a peptide dose? Use our beginner-friendly peptide dosage, blend, and accumulation calculators. Enter vial size, reconstitution volume, and target dose to get exact draw volumes instantly. No guesswork, just clear guidance that helps prevent common mistakes.
