Reviewed by the PeptideMind Team · Updated August 5, 2026

Pinealon Peptide Dosage Guide, Benefits & Side Effects

Pinealon (EDR tripeptide) dosage guide: how much to take for sleep and memory, plus benefits, side effects, and protocols backed by research.

Basic Dosage Calculator

Calculate peptide doses with our visual syringe guide.

Peptide per vial

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Pinealon Dosage Schedule

A short cycle that supports your natural sleep wake rhythm while helping protect brain cells from stress related damage. A solid pick if better, more consistent sleep is the main goal.

Pinealon for Restful Sleep and Brain Protection

3 weeks split across multiple phases, focused on sleep quality.

Daily dosing

Taken daily.

1.5 mg per injection

1.5 mg per injection, given just under the skin in the abdomen or thigh.

Pinealon is a very short peptide studied for protecting brain cells and supporting memory. It may also help reduce stress related damage in the brain and support your body's natural sleep wake cycle, and it's usually taken in short cycles. Sources disagree on whether morning or evening dosing better supports the sleep wake rhythm, so pick a consistent time of day and stick with it for the cycle.

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What is Pinealon?

Pinealon is a short, lab-made peptide developed to support the pineal gland and brain function generally. It works by interacting directly with your cells' genetic material to support brain protection, antioxidant defenses, and healthy circadian (sleep-wake) rhythms. It's used in research for cognitive support, protecting the brain from age-related decline, and resetting disrupted sleep cycles.

Cognitive

Research targets include neural signaling, memory formation, and mental clarity pathways.

Longevity

Research targets include cellular aging, senescence, and long-term healthspan.

Sleep

Research targets include circadian rhythm regulation and sleep quality pathways.

Cognitive

pin

Longevity

Where to buy Pinealon

Compare real-time Pinealon prices from vendors with third-party lab testing (COA), purity, and legitimacy, so you know exactly who's trustworthy before you buy.

Dose size

20mgfrom $55 · 8 vendors10mgfrom $55 · 2 vendors

Peptide Mind's top pick

Chosen on COA purity, third-party testing, and service, no paid placements.

Protide Health

99.62% COA|5.0|In stock

Buy now at Protide Health Compared across 10 vendors

3 of 10 vendors third-party tested, COA purity 99.2–99.72% across published labs. Prices re-checked 17 days ago.

What Does Pinealon Do?

It travels into the nucleus of brain cells and interacts with DNA to help switch on genes involved in fighting off cell-damaging molecules and preventing unwanted cell death. This helps protect brain cells from the kind of stress and damage linked to aging and injury.

Enters brain cell nuclei

Interacts with DNA inside brain cells.

Fights cell-damaging molecules

Helps switch on genes that neutralize damaging molecules.

Prevents unwanted cell death

Protects brain cells from stress and damage linked to aging and injury.

Frequently Asked Questions About Pinealon

Straight answers on reconstitution, dosing, and safety, everything you need to research with confidence. For research reference only.

Pinealon is a synthetic tripeptide with the amino acid sequence Glutamate-Aspartate-Arginine (Glu-Asp-Arg, abbreviated EDR). It was developed by the Khavinson research group in St. Petersburg as a synthetic version of an active fragment found in pineal gland extracts (such as Epithalamin), part of a broader family of 'peptide bioregulators.' Rather than acting through a typical membrane receptor, it is proposed to cross the cell membrane and blood-brain barrier and interact directly with neuronal chromatin (DNA), potentially influencing gene expression related to neuroprotection, antioxidant defense, and circadian rhythm regulation. This mechanism is based mainly on the Khavinson group's own publications and preclinical models; independent replication in mainstream Western pharmacology literature is limited.

There is no FDA-approved or clinically established human dose. Reports vary widely across sources: some describe published research protocols using roughly 0.1-0.3 mg per administration, while online research-community discussions more often describe subcutaneous doses in the 1-10 mg per day range, typically run for short cycles of 5-20 consecutive days, followed by an off-cycle break of one to several months. Because dosing conventions are inconsistent and not derived from controlled human trials, any figures found online should be treated as anecdotal community practice rather than validated medical guidance.

Pinealon has been used or sold in injectable (subcutaneous), oral (capsule/tablet), oral spray, and intranasal spray forms. The subcutaneous route has the strongest link to the original published research from the Khavinson group, though a notable published human report (a small study in patients with traumatic brain injury sequelae and cerebrasthenia) used an oral route. Comparative bioavailability data between routes has not been well established, so claims that one route is more effective than another are not strongly supported by evidence.

Available human data is limited, and there is no long-term safety database. Anecdotal reports from research/biohacking communities most commonly mention mild, transient effects such as vivid dreams, mild headache, temporary fatigue, and injection-site redness or itching. Published reports describe it as generally well tolerated with few adverse events, but the absence of frequently reported problems is not the same as proven long-term safety, especially since Pinealon is not approved or regulated for human use.

Pinealon is not FDA-approved for any human medical use and is not a controlled substance under the U.S. Controlled Substances Act. It is commonly sold labeled 'for research use only, not for human consumption,' typically to researchers or institutions rather than as a consumer health product. Regulatory status can vary by country and can change, so anyone considering it should check current local regulations rather than relying on vendor marketing claims.

As with most peptides sold in research contexts, the lyophilized (freeze-dried) powder is generally recommended to be stored frozen (around -20°C) for long-term stability before reconstitution. Once reconstituted with bacteriostatic or sterile water, it is commonly recommended to refrigerate (2-8°C) and use within about 2-4 weeks, since peptides degrade faster at room temperature and lose potency over time. Exact stability data specific to Pinealon has not been independently verified through published studies, so these are general peptide-handling conventions rather than manufacturer-verified shelf-life claims.

Within research and biohacking communities, Pinealon is frequently discussed alongside other Khavinson-group bioregulator peptides, most notably Epitalon (Epithalon), which is studied for effects related to the sleep-melatonin cycle and telomerase activity, and sometimes DSIP (delta sleep-inducing peptide). These are often grouped together informally as 'pineal peptides' for sleep- and aging-related research interest, but there is no clinical trial evidence establishing safety or effects of combining them, and any such stacking reflects community practice rather than validated protocols.

The evidence base for Pinealon comes almost entirely from a single research lineage (the Khavinson Institute of Bioregulation and Gerontology), including preclinical (animal/cell) studies and a small number of published human studies, such as a study in patients with traumatic brain injury consequences. This research reports neuroprotective and antioxidant effects and modulation of circadian/melatonin-related pathways, but independent replication by other research groups is essentially absent from peer-reviewed Western literature, and no large controlled human trials exist. As a result, claims about its effectiveness for memory, sleep, or brain health in humans should be treated as preliminary and unproven rather than established, and it is not intended or approved as a treatment for any condition.

Pinealon Reconstitution Guide

Pinealon is supplied as a freeze-dried powder that must be mixed into a injectable solution before use. Available in 10mg, 20mg sizes.

10 mg lyophilized vial

Pinealon is supplied as a freeze-dried powder that must be mixed into a injectable solution before use. Available in 10mg, 20mg sizes.

2 mL bacteriostatic water

Adding 2 mL of bac water to a 10 mg vial gives a workable concentration for syringe dosing. More water makes microdosing easier; less concentrates each draw.

Storage after mixing

Freeze the dry powder for up to two years. Once mixed with bacteriostatic water, refrigerate and use within about a month. After reconstitution, keep the vial refrigerated and use within 30 days.

Reconstitution steps

Remove caps & sanitize stoppers

Attach needle to syringe

Draw 2 mL bacteriostatic water

Insert needle into Pinealon vial

Release water gently down the side

Swirl to dissolve — don't shake

Check for full dissolution

Refrigerate Pinealon — good for 30 days

Chemical Makeup

Amino Acids

Pinealon

Glu

Position 1

Asp

Position 2

Arg

Position 3

Amino acid sequence

Glutamic acidPosition 1

Aspartic acidPosition 2

ArgininePosition 3

What Are the Benefits of Pinealon?

What research says it may help with, and how it works in the body.

neuroprotection

: When injected under the skin, it travels through the bloodstream and reaches brain tissue, where it can help protect nerve cells.
: In a study of 72 patients, taking 5mg daily led to significant improvements in thinking and mental performance.
: Blocks a protein involved in cell death (caspase-3), reduces harmful reactive molecules (ROS, or free radicals) that damage cells, and protects nerve cells from being overstimulated to the point of injury.

cognitive

: Improves the function of brain chemical messengers, nearly doubling serotonin levels in lab cell studies.
: A dose of 100 nanograms per kilogram of body weight was found to be the most effective for improving spatial learning (like navigating and remembering locations), a function tied to a brain region called the hippocampus.

antiaging

: In a 32-patient study, significant improvements in markers of aging were seen over a 20 to 30 day period.
: Increases a hormone called irisin, which is linked to protecting the caps on the ends of chromosomes (telomeres) and improving the energy-producing parts of cells (mitochondria).

What Are the Side Effects of Pinealon?

Who should avoid it, warning signs to watch for, and what to know before combining it with other compounds.

Who Should Avoid It

: Pregnancy
: Lactation

Stop Right Away If You Notice

: Severe headaches or mood changes
: Persistent injection site reactions
: Signs of infection at injection site
: Unusual neurological symptoms
: Allergic reactions

Milder Signs to Watch For

Discolored powder (yellow or brown) indicates degradation from heat or moistureCloudy solution indicates contamination

Was it helpful?Yes

Is Pinealon FDA approved?

Pinealon is not an FDA-approved drug. It is intended for research purposes only and is not approved for human consumption, diagnostic, or therapeutic use.

Research Use Only

Pinealon has not been evaluated by the FDA for safety or efficacy in humans.

No Clinical Oversight

Not manufactured under FDA-regulated quality or clinical standards.

Unregulated Sourcing

Purity, dosing, and sourcing are not verified through FDA testing or oversight.

A smartphone screen displaying FDA status for a drug, indicating "FDA approved drug: No" and "Classification: Research use only." A banner notes "Research Only" with the disclaimer "not for human use," emphasizing its intended use for laboratory and analytical purposes.

Pinealon Cycle Length: How Long Should It Last?

This breaks down how long a typical Pinealon cycle runs and what research suggests happens at each stage. Research shows that staying on a peptide continuously, without a break, may make it less effective over time.

That's why most research protocols build in a break between cycles, often called a washout period, to let the body reset before starting again.

Week 1-2: Improved mental clarity and focus
Week 2-3: Enhanced cognitive processing and memory
Week 3-4: Peak neuroprotective and anti-aging benefits

Pinealon Research References

It is a preclinical compound

4Research references

Pinealon

Pinealon is a preclinical compound

Aging Study - Polymorbidity Patients

32 patients aged 41-83; variable dosing; 30 days. Significant improvements in biological aging biomarkers with anabolic effects.

2015

Diabetic Rat Model - Spatial Learning

100 ng/kg optimal dose; 14 days. Enhanced NMDA receptor expression in hippocampus with neuroplasticity improvements.

2020

5xFAD Alzheimer's Mouse Model

400 μg/kg IP daily; 28 days. Prevention of dendritic spine loss through MAPK/ERK pathway modulation.

2021

Human Clinical Trial - Post-Traumatic Cerebrasthenia

72 patients aged 30-74; 0.2mg twice daily; 20-30 days. Improved memory, reduced headache severity, and enhanced emotional stability compared to controls.

2015

Pinealon Peptide Dosage Guide, Benefits & Side Effects

Pinealon Dosage Schedule

What is Pinealon?