Weight
5-Amino-1MQ
Longevity
0
Amino acids
Molecular weight
Peptide
Type
5-Amino-1MQ (5-amino-1-methylquinolinium) is a selective small-molecule inhibitor of nicotinamide N-methyltransferase (NNMT), an enzyme that depletes intracellular NAD+ and suppresses metabolic rate. Note: although classified as 'PEPTIDE' here per schema constraints, 5-Amino-1MQ is a quinolinium salt small molecule, not a peptide. The authoritative free-cation mass (PubChem CID 950107, C10H11N2+) is 159.21 Da; the commonly supplied iodide salt form (C10H11N2·I) has a molecular weight of 286.11 Da. By blocking NNMT, it elevates NAD+ levels, activates sirtuin pathways, and shifts cellular energy balance toward fat oxidation, making it a focus of preclinical research on obesity, metabolic dysfunction, and longevity. No human pharmacokinetic data are established; half-life data derive from rat studies only (3.80h IV, 6.90h oral).
Top researched benefits
Overview of 5-Amino-1MQ
5-Amino-1MQ selectively inhibits NNMT, preventing the enzyme from consuming S-adenosyl-L-methionine (SAM) and depleting NAD+ stores. The resulting rise in intracellular NAD+ activates SIRT1 and PGC-1α, driving mitochondrial biogenesis, enhanced fatty acid oxidation, and increased basal metabolic rate.
longevity
- Significantly increases cellular NAD+ levels by inhibiting NNMT enzyme, supporting cellular energy production and DNA repair mechanisms essential for healthy aging.
- Improves mitochondrial biogenesis and efficiency, enhancing cellular energy production and reducing oxidative stress markers associated with aging.
- Enhanced NAD+ availability supports sirtuins and other longevity pathways involved in DNA repair, cellular maintenance, and stress resistance.
metabolism
- Improvement through enhanced mitochondrial efficiency.
- Enhancement via cellular energy optimization.
- Improved glucose/fat substrate switching.
weight loss
- Supports increased basal metabolic rate.
- Improvements in fat burning capacity.
Typical Dose
50–100mg per day (oral capsule); 75mg midpoint used as reference dose
Frequency
once daily
Cycle Duration
8 weeks on, 4 weeks off
Storage
Lyophilized powder: store at -20°C, stable up to 24 months. Reconstituted solution: refrigerate at 2–8°C, use within 2–4 weeks. Keep away from light; do not refreeze after reconstitution.
No visual available
Due to this peptide having no amino acids, there is no molecular chain to display.
Chemical Makeup
Key benefits
Reduces white adipose tissue mass by ~35% and adipocyte size by over 30% in diet-induced obese mice by shifting cellular energy metabolism toward fat oxidation over fat storage
Elevates intracellular NAD+ levels by 1.2–1.6-fold in adipose tissue, fueling mitochondrial ATP production and improving overall cellular energy availability
Activates SIRT1 and PGC-1α signaling, promoting mitochondrial biogenesis and improving metabolic flexibility without altering food intake
Increases grip strength by approximately 25% in preclinical models; combines additively with exercise for up to 60% improvement in muscle performance metrics
Community interest
This peptide is still gaining traction in the community.
NNMT Inhibitor | Longevity & Metabolic Enhancement
This overview is informational and based on aggregated descriptions from studies and user reports.
Was it helpful?YesNo5-Amino-1MQ Molecular Information
View the scientifc details of 5-Amino-1MQ.
Molecular Weight
159.21DaChain Length
0Amino AcidsType
Peptide5-Amino-1MQ Protocols
Oral capsule is the primary and most widely used delivery route for 5-Amino-1MQ, supported by an LC-MS/MS rat pharmacokinetic study (PubMed 34304009) confirming 38.4% oral bioavailability. Capsules are taken once daily in the morning, with or without food, at doses of 50–150 mg to compensate for first-pass hepatic metabolism.
| Goal | Dosage | Frequency | Route |
|---|---|---|---|
| Fat loss — beginner | 50 | 1 day range | Oral (morning, with or without food) |
| Fat loss — standard | 100 | 1 day range | Oral (morning, with or without food) |
| Fat loss — split dose (75 mg BID = 150 mg/day total) | 75 | 2 day range | Oral (morning and midday) |
| Metabolic enhancement — high dose | 150 | 1 day range | Oral (morning, with or without food) |
| NAD+ pathway / longevity support | 100 | 1 day range | Oral (morning, with or without food) |
| Obesity / insulin resistance | 50 | 2 day range | Oral (morning and midday) |
Reconstitution Instructions
Materials needed:
Steps to reconstitute
- Confirm your starting dose — 50 mg/day for the first 1–2 weeks as a tolerance assessment before advancing.
- Take the capsule in the morning, with or without food. Morning timing aligns NNMT inhibition with peak metabolic activity during the day.
- Swallow the capsule whole with a full glass of water. Do not open, crush, or dissolve the capsule.
- Take at the same time each day to maintain consistent plasma levels given the compound's half-life of approximately 6.9 hours after oral dosing.
- If splitting the daily dose, take the second dose no later than midday to sustain inhibition without disrupting evening metabolic cycling.
- Do not exceed 150 mg in a single daily total. A 75 mg BID protocol (morning and midday) totals 150 mg/day — equivalent to the labeled high-dose single daily protocol.
- Run 8–12 weeks on, then take 4–8 weeks off before resuming. Mark the start date to track cycle length.
5-Amino-1MQ Cycle
The 5-Amino-1MQ Cycle section explains how long a typical cycle lasts and what to expect during each phase. Over time, your body can become less responsive with continuous use.
Taking breaks between cycles may help maintain effectiveness and support better overall results.
- Week 1-2
- Gradual energy increase and mental clarity
- Week 2-4
- Enhanced exercise performance and recovery
- Week 4-8
- Improved metabolic markers and body composition
- Week 8-12
- Sustained longevity benefits and cellular health improvements
Dosing tools
5-Amino-1MQ Peptide Dosage Calculator
Calculate peptide doses with our visual syringe guide.
mg
Enter the total amount of peptide in the vial in milligrams (as stated on the label).
The dose you want to inject per administration, in mcg or mg.
1,000 mcg = 1 mg
1 mL
2 mL
3 mL
5 mL
Custom
Volume of bacteriostatic water you add to reconstitute the powder. Use BAC water for preservation.
Injection Results
Based on your vial and dilution inputs.
5-Amino-1MQ
SINGLE COMPOUNDVolume per injection
0.05
mL
Concentration
10.00
mg/mL
Doses per vial
20
doses
Total injections per vial
20 injections
How it works
Based on a 10 mg 5-Amino-1MQ vial diluted with 1 mL of bacteriostatic water, each 500 mcg injection equals 0.05 mL.
1mL / 100 units
5 units
0.050 mL
Reference Guide
Dosing Cycle
- Peptide
- 5-Amino-1MQ
- Dosing
- 50–100mg per day (oral capsule); 75mg midpoint used as reference dose
- Dosing Frequency
- once daily
- Cycle Duration
- 8 weeks on, 4 weeks off
- Storage
- Lyophilized powder: store at -20°C, stable up to 24 months. Reconstituted solution: refrigerate at 2–8°C, use within 2–4 weeks. Keep away from light; do not refreeze after reconstitution.
Note: Triple agonist; microdose for fewer side effects
Reconstitution Tips
- Use bacteriostatic water (BAC) — contains 0.9% benzyl alcohol for preservation
- Inject water slowly — aim down the vial wall, not directly onto powder
- Never shake — gently swirl or roll the vial until dissolved
- Store properly — refrigerate at 2-8°C after reconstitution
- Use within 28 days — most reconstituted peptides remain stable for about 4 weeks
- Keep sterile — always clean vial tops with alcohol before drawing
Peptide Interactions
Research suggestions of 5-Amino-1MQ interactions with other common peptides and substances.
Healing
bpc
Longevity
BPC-157
COMPATIBLE
No known interactions; different mechanisms with potential complementary benefits.
Side effects
Contraindications
Pregnancy or breastfeeding
Pre-existing metabolic conditions (consult healthcare provider)
Stop signs
Severe gastrointestinal upset or persistent nausea
Unusual fatigue or weakness (opposite of expected effect)
Persistent headaches or dizziness
Sleep disturbances or insomnia
Allergic reactions (rash, swelling, difficulty breathing)
Significant mood changes or anxiety
Bad signs
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Frequently asked questions
What is a peptide dosage calculator?
A peptide dosage calculator is a free tool that converts your vial size, bacteriostatic water volume, and target dose into an exact syringe draw volume. Instead of doing the reconstitution math by hand, you enter three inputs and instantly get the concentration of your solution and how many milliliters or syringeunits to draw. This calculator works for single peptide compounds and multi-peptide blends.
How do I calculate peptide dosage from a vial?
To calculate your peptide dose, divide the total peptide content of your vial in micrograms by the volume of bacteriostatic water you added in milliliters. This gives you your solution concentration in mcg/mL. Then divide your target dose by that concentration to get your draw volume. For example, a 5mg (5,000 mcg) vial reconstituted with 2mL of BAC water gives a concentration of 2,500 mcg/mL. A 250 mcg dose would require drawing 0.1mL. This calculator automates all of those steps instantly.
How much Bacteriostatic water should I add to a peptide vial?
Most people add 2mL to 3mL of bacteriostatic water per vial, but the right amount depends on the dose you want to draw and the syringe size you are using. Adding 1mL to a 5mg vial gives you a concentration of 5,000 mcg/mL, making each dose very small in volume. Adding 2mL gives you 2,500 mcg/mL, which is easier to measure on a standard insulin syringe. A general guideline is to choose a volume that puts your typical dose somewhere between 10 and 30 units on a U-100 syringe. Use the calculator above to test different water volumes and find what works for your dose.
How are peptides different from proteins?
Both are made of amino acids, but peptides are much smaller than proteins. Because of their tiny size, peptides can act like tiny messengers in the body, sending specific signals to your cells to tell them exactly what to do.
5-Amino-1MQResearch References
5-Amino-1MQ is a preclinical compound
5-Amino-1MQ
5-Amino-1MQ is a preclinical compound
Mouse Obesity Study - Neelakantan et al.
Diet-induced obese mice showed 5.1% weight loss, 30% adipocyte size reduction at 60mg/kg/day. Human equivalent ~5mg/kg/day using FDA allometric scaling.
2018
Rat Pharmacokinetics Study
Demonstrated 38.4% oral bioavailability, half-life 6.9hr (oral) and 3.8hr (IV). Supports oral administration viability.
2021
Muscle Function in Aged Mice
40% grip strength improvement in sedentary mice, 60% with exercise at 10mg/kg/day SubQ for 8 weeks. No adverse effects observed.
2024
Calculate peptide dosages
Learning how to calculate a peptide dose? Use our beginner-friendly peptide dosage, blend, and accumulation calculators. Enter vial size, reconstitution volume, and target dose to get exact draw volumes instantly. No guesswork, just clear guidance that helps prevent common mistakes.
