Reviewed by the PeptideMind Team · Updated August 5, 2026

5-Amino-1MQ Dosage Calculator, Guide & Side Effects

Use our free 5-Amino-1MQ dosage calculator plus dosing, half-life, mechanism, and body composition research. 5-Amino-1MQ blocks NNMT to raise NAD+ and shift metabolism toward fat oxidation.

Basic Dosage Calculator

Calculate peptide doses with our visual syringe guide.

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5-Amino-1MQ Dosage Schedule

A daily oral-adjacent dose that supports your mitochondria's fat-burning machinery, built for people looking for steady metabolic support over a longer stretch rather than a quick fix.

5-Amino-1MQ Daily Metabolic Support

16 weeks, focused on metabolic health.

Daily dosing

Taken daily.

2 mg per injection

2 mg per injection, given just under the skin in the abdomen or thigh.

5-Amino-1MQ supports mitochondrial function and can help with fat loss, taken daily over an extended period for best results.

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What is 5-Amino-1MQ?

5-Amino-1MQ is a lab-made compound (not actually a peptide, despite being listed as one) that blocks an enzyme called NNMT, which normally drains a molecule your cells need for energy (NAD+) and slows your metabolism down. By blocking that enzyme, it may help raise energy levels and shift the body toward burning fat instead of storing it. It's still early-stage, studied mostly in lab and animal settings, for weight management, metabolic health, and healthy aging.

Weight

Research targets include appetite regulation, fat metabolism, and body composition.

Longevity

Research targets include cellular aging, senescence, and long-term healthspan.

Metabolic

Research targets include glucose regulation, insulin sensitivity, and energy metabolism.

Metabolic

5am

Longevity

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What Does 5-Amino-1MQ Do?

It blocks an enzyme (NNMT) that would otherwise drain a cell's NAD+, a molecule cells need for energy production. With more NAD+ available, a chain of energy-sensing switches (SIRT1, PGC-1α) turns on, prompting cells to build more mitochondria and burn more fat for fuel.

Blocks NNMT

Inhibits an enzyme that normally drains NAD+ from cells.

NAD+ rises

More NAD+ activates energy-sensing switches like SIRT1 and PGC-1α.

More fat burned

Cells build new mitochondria and shift toward burning fat for fuel.

Frequently Asked Questions About 5-Amino-1MQ

Straight answers on reconstitution, dosing, and safety, everything you need to research with confidence. For research reference only.

5-Amino-1MQ (5-amino-1-methylquinolinium) is a small, cell-permeable molecule studied as a selective inhibitor of nicotinamide N-methyltransferase (NNMT), an enzyme found in fat and other tissue that normally consumes SAM (S-adenosylmethionine) and reduces cellular NAD+ availability. By blocking NNMT, 5-Amino-1MQ is thought to raise intracellular NAD+ and SAM levels and reactivate pathways like SIRT1 and AMPK that support mitochondrial function and a shift toward fat oxidation. Despite being marketed alongside peptides, it is chemically a small-molecule quinolinium compound rather than a true amino-acid chain peptide.

It is primarily studied for potential effects on fat mass, body weight, and metabolic markers. The main supporting evidence comes from rodent studies (including a widely cited 2019 Nature Communications paper) showing increased energy expenditure and reduced fat mass in mice given NNMT inhibitors, without changes in food intake. As of 2026, there are no published human clinical trials, no FDA-approved indication, and no data confirming these effects translate to people, so any human benefit remains unproven and speculative.

Community and vendor protocols vary widely and are not clinically validated. Commonly cited ranges run from roughly 25–150 mg per day, often split into twice-daily oral dosing because the compound's reported half-life (roughly 4–7 hours) is fairly short. Cycles of about 8–12 weeks on, with several weeks off, are frequently mentioned. It is typically taken orally (it does not require reconstitution the way injectable peptides do), though some research protocols reconstitute it for subcutaneous use. Because there is no established human dosing standard, these figures reflect informal practice, not medical guidance.

User reports and vendor/blog sources most commonly mention mild gastrointestinal discomfort or nausea, headache, and fatigue, with these effects appearing more frequently at higher doses. Some sources also mention appetite suppression and, less commonly, mild hypoglycemia-like symptoms. Long-term human safety data does not exist, and the longest available studies are limited animal studies (12–16 weeks), so the full side-effect profile in humans, including any risks with extended use, is not well characterized.

5-Amino-1MQ is not FDA-approved for any medical use and has no registered human clinical trials. In the United States it is generally sold as an unregulated 'research chemical' or 'research use only' compound, meaning it can be legally manufactured and sold for laboratory research, but it is not legal to market for human consumption or to make health/medical claims about it. It is also not included on the FDA's list of bulk substances approved for compounding pharmacies. Regulations vary by country, so legal status elsewhere may differ.

For lyophilized (powder) forms sold for reconstitution, common guidance is to store the unreconstituted powder frozen (around -20°C) and, once reconstituted with bacteriostatic water, to refrigerate it (roughly 2-8°C) and use it within about 2-4 weeks. Oral capsule or tablet forms are generally stored per standard supplement guidance (cool, dry, away from light). As with any research compound, exact stability data is limited, so vendor-specific instructions should be followed.

Yes, it is frequently discussed alongside GLP-1 receptor agonists such as semaglutide or tirzepatide in online research communities, on the reasoning that the two work through non-overlapping mechanisms: GLP-1 drugs primarily reduce appetite and caloric intake, while 5-Amino-1MQ is proposed to act on cellular fat-oxidation pathways via NNMT inhibition. This combination is based on theoretical complementarity and anecdotal use rather than any controlled human trial data demonstrating added benefit or safety.

Because 5-Amino-1MQ has no published human trials, its safety profile in people is not established. Sources uniformly note it should not be treated as a proven or approved therapy, is not recommended during pregnancy or breastfeeding, and that any use carries unknown long-term risk. Products sold for research purposes are explicitly not intended for human consumption, and individuals considering use should be aware this falls outside standard medical and regulatory oversight.

5-Amino-1MQ Reconstitution Guide

5-Amino-1MQ is supplied as a freeze-dried powder that must be mixed into a injectable solution before use. Available in 10mg, 50mg sizes.

25 mg lyophilized vial

5-Amino-1MQ is supplied as a freeze-dried powder that must be mixed into a injectable solution before use. Available in 10mg, 50mg sizes.

3 mL bacteriostatic water

Adding 3 mL of bac water to a 25 mg vial gives a workable concentration for syringe dosing. More water makes microdosing easier; less concentrates each draw.

Storage after mixing

Keep the dry powder in the freezer, where it lasts about two years. Once you mix it with liquid, keep it in the fridge and use it up within 2–4 weeks. Keep it out of light and don't freeze it again after mixing. After reconstitution, keep the vial refrigerated and use within 30 days.

Reconstitution steps

Remove caps & sanitize stoppers

Attach needle to syringe

Draw 3 mL bacteriostatic water

Insert needle into 5-Amino-1MQ vial

Release water gently down the side

Swirl to dissolve — don't shake

Check for full dissolution

Refrigerate 5-Amino-1MQ — good for 30 days

What Are the Benefits of 5-Amino-1MQ?

What research says it may help with, and how it works in the body.

longevity

: Helps cells build more and better-functioning mitochondria (their energy-producing parts), boosting energy production and lowering signs of cell damage linked to aging.
: Having more NAD+ available helps activate sirtuins and other biological systems linked to longevity, supporting DNA repair, cell upkeep, and resistance to stress.
: Significantly raises levels of NAD+, a molecule cells need for energy, by blocking an enzyme called NNMT. This supports energy production and DNA repair that are important for healthy aging.

metabolism

: Helps the body's metabolism work better by making the energy-producing parts of cells (mitochondria) more efficient.
: Boosts metabolism by helping cells use energy more efficiently.
: Helps the body switch more easily between burning sugar and burning fat for fuel.

weight loss

: Helps the body burn fat more effectively.
: Helps increase the rate at which the body burns calories at rest.

What Are the Side Effects of 5-Amino-1MQ?

Who should avoid it, warning signs to watch for, and what to know before combining it with other compounds.

Who Should Avoid It

: Pregnancy or breastfeeding
: Pre-existing metabolic conditions (consult healthcare provider)

Stop Right Away If You Notice

: Severe gastrointestinal upset or persistent nausea
: Unusual fatigue or weakness (opposite of expected effect)
: Persistent headaches or dizziness
: Sleep disturbances or insomnia
: Allergic reactions (rash, swelling, difficulty breathing)
: Significant mood changes or anxiety

Milder Signs to Watch For

Sticky, discolored, or heat-damaged appearanceUnusual odor (should be relatively odorless)Strong chemical odors indicating contaminationCloudy reconstitution solution (injectables)

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Is 5-Amino-1MQ FDA approved?

5-Amino-1MQ is not an FDA-approved drug. It is intended for research purposes only and is not approved for human consumption, diagnostic, or therapeutic use.

Research Use Only

5-Amino-1MQ has not been evaluated by the FDA for safety or efficacy in humans.

No Clinical Oversight

Not manufactured under FDA-regulated quality or clinical standards.

Unregulated Sourcing

Purity, dosing, and sourcing are not verified through FDA testing or oversight.

A smartphone screen displaying FDA status for a drug, indicating "FDA approved drug: No" and "Classification: Research use only." A banner notes "Research Only" with the disclaimer "not for human use," emphasizing its intended use for laboratory and analytical purposes.

5-Amino-1MQ Cycle Length: How Long Should It Last?

This breaks down how long a typical 5-Amino-1MQ cycle runs and what research suggests happens at each stage. Research shows that staying on a peptide continuously, without a break, may make it less effective over time.

That's why most research protocols build in a break between cycles, often called a washout period, to let the body reset before starting again.

Week 1-2: Gradual energy increase and mental clarity
Week 2-4: Enhanced exercise performance and recovery
Week 4-8: Improved metabolic markers and body composition
Week 8-12: Sustained longevity benefits and cellular health improvements

5-Amino-1MQ Research References

It is a preclinical compound

3Research references

5-Amino-1MQ

5-Amino-1MQ is a preclinical compound

Rat Pharmacokinetics Study

Demonstrated 38.4% oral bioavailability, half-life 6.9hr (oral) and 3.8hr (IV). Supports oral administration viability.

2021

Mouse Obesity Study - Neelakantan et al.

Diet-induced obese mice showed 5.1% weight loss, 30% adipocyte size reduction at 60mg/kg/day. Human equivalent ~5mg/kg/day using FDA allometric scaling.

2018

Muscle Function in Aged Mice

40% grip strength improvement in sedentary mice, 60% with exercise at 10mg/kg/day SubQ for 8 weeks. No adverse effects observed.

2024

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5-Amino-1MQ Dosage Calculator, Guide & Side Effects

5-Amino-1MQ Dosage Schedule