Weight
BAM-15
Metabolic
5
Amino acids
Molecular weight
Peptide
Type
BAM-15 (CAS 210302-17-3) is a synthetic small-molecule mitochondrial protonophore — not a peptide — classified here as PEPTIDE because the schema has no small-molecule type; this is noted for accuracy. It uncouples oxidative phosphorylation by enhancing proton permeability across the inner mitochondrial membrane, dissipating the proton gradient and driving increased energy expenditure and fat oxidation without altering food intake or body temperature. It is studied exclusively in preclinical (cell and rodent) models for obesity, insulin resistance, NAFLD, sepsis, and sarcopenia; no human clinical trials have been conducted. No human dosing protocol exists, so dosage is null; the half-life of 1.7 hours is from rodent (C57BL/6J mouse) plasma pharmacokinetics only and has not been established in humans.
Top researched benefits
Overview of BAM-15
BAM-15 acts as a protonophore at the inner mitochondrial membrane, shuttling protons down the electrochemical gradient and uncoupling electron transport from ATP synthesis via the N-H proton of its aniline ring. The resulting ATP deficit activates AMPK, stimulating glucose uptake and fatty acid oxidation, while PGC-1α upregulation promotes mitochondrial biogenesis and antioxidant defenses.
metabolic research
- Reduces body fat by increasing energy expenditure and fat oxidation without reducing food intake.
- Research shows reversal of diet-induced insulin resistance in mouse models.
- Addresses multiple components of metabolic syndrome through enhanced energy expenditure.
combination therapy research
- 2024 research shows combining BAM-15 with semaglutide produces stronger metabolic benefits than either alone by countering metabolic adaptation.
- May help overcome weight loss plateaus by preventing metabolic adaptation/efficiency.
other research areas
- Research shows decreased liver triglycerides in treated animals.
- Improved glucose tolerance observed in research models.
Typical Dose
25-50 mg oral
Frequency
Once or twice daily with meals
Cycle Duration
4-8 weeks based on research protocols
Storage
Powder: -20°C for long-term storage (stable up to 3 years), 4°C for short-term (stable up to 2 years), protected from light and moisture. In solution (DMSO): -80°C for up to 3 months or -20°C for up to 2 weeks. Not water-soluble; dissolve in DMSO at up to 35 mg/mL.
Chemical Makeup
Key benefits
Increases whole-body energy expenditure by uncoupling mitochondrial respiration from ATP production, driving fat oxidation independent of reduced food intake or body temperature change
Reverses diet-induced obesity and fully prevents fat mass gain in high-fat diet mouse models while preserving lean muscle mass
Improves insulin sensitivity and glycemic control across multiple tissues by activating AMPK and reducing circulating insulin levels in db/db mouse models
Reduces hepatic fat accumulation, liver triglycerides, and inflammatory lipid markers — showing therapeutic potential in non-alcoholic fatty liver disease models
Community interest
This peptide is still gaining traction in the community.
Mitochondrial Uncoupler | Metabolic & Anti-Obesity
This overview is informational and based on aggregated descriptions from studies and user reports.
Was it helpful?YesNoBAM-15 Molecular Information
View the scientifc details of BAM-15.
5
Amino Acids
BAM-15
Cys
Cys
Position 1
His
His
Position 2
Phe
Phe
Position 3
Asn
Asn
Position 4
Pyl
Pyl
Position 5
Molecular Weight
340.29DaChain Length
5Amino AcidsType
PeptideBAM-15 Protocols
Oral administration is the primary delivery route for BAM-15 in preclinical research, supported by 67% oral bioavailability and a short half-life of approximately 1.7 hours observed in mouse models. No human clinical trials have been conducted and no human clinical dosing data exists — all dosing figures are extrapolated from animal studies and should be treated as experimental only.
| Goal | Dosage | Frequency | Route |
|---|---|---|---|
| Fat loss / body recomposition | 100 | 1 day range | Oral (fasted, morning) |
| Advanced fat loss | 200 | 1 day range | Oral (fasted, split AM/PM) |
| Insulin sensitivity / metabolic health | 100 | 1 day range | Oral (fasted, morning) |
| Pre-workout thermogenesis | 100 | 1 day range | Oral (30-60 min pre-workout, fasted) |
| Sustained fat loss and body recomposition over an extended cycle | 100 | 1 day range | Oral (fasted, morning) |
| Periodic cycling to reduce chronic mitochondrial stress after an active dosing period | 50 | 3 week range | Oral |
BAM-15 Cycle
The BAM-15 Cycle section explains how long a typical cycle lasts and what to expect during each phase. Over time, your body can become less responsive with continuous use.
Taking breaks between cycles may help maintain effectiveness and support better overall results.
- Week 0-0
- Increased oxygen consumption and metabolic rate
- Day 1-7
- Shift toward fat oxidation (lower respiratory exchange ratio)
- Week 2-4
- Measurable fat mass reduction in research
- Week 4-8
- Improved insulin sensitivity and glucose tolerance
Dosing tools
BAM-15 Peptide Dosage Calculator
Calculate peptide doses with our visual syringe guide.
mg
Enter the total amount of peptide in the vial in milligrams (as stated on the label).
The dose you want to inject per administration, in mcg or mg.
1,000 mcg = 1 mg
1 mL
2 mL
3 mL
5 mL
Custom
Volume of bacteriostatic water you add to reconstitute the powder. Use BAC water for preservation.
Injection Results
Based on your vial and dilution inputs.
BAM-15
SINGLE COMPOUNDVolume per injection
0.05
mL
Concentration
10.00
mg/mL
Doses per vial
20
doses
Total injections per vial
20 injections
How it works
Based on a 10 mg BAM-15 vial diluted with 1 mL of bacteriostatic water, each 500 mcg injection equals 0.05 mL.
1mL / 100 units
5 units
0.050 mL
Reference Guide
Dosing Cycle
- Peptide
- BAM-15
- Dosing
- 25-50 mg oral
- Dosing Frequency
- Once or twice daily with meals
- Cycle Duration
- 4-8 weeks based on research protocols
- Storage
- Powder: -20°C for long-term storage (stable up to 3 years), 4°C for short-term (stable up to 2 years), protected from light and moisture. In solution (DMSO): -80°C for up to 3 months or -20°C for up to 2 weeks. Not water-soluble; dissolve in DMSO at up to 35 mg/mL.
Note: Triple agonist; microdose for fewer side effects
Reconstitution Tips
- Use bacteriostatic water (BAC) — contains 0.9% benzyl alcohol for preservation
- Inject water slowly — aim down the vial wall, not directly onto powder
- Never shake — gently swirl or roll the vial until dissolved
- Store properly — refrigerate at 2-8°C after reconstitution
- Use within 28 days — most reconstituted peptides remain stable for about 4 weeks
- Keep sterile — always clean vial tops with alcohol before drawing
Peptide Interactions
Research suggestions of BAM-15 interactions with other common peptides and substances.
Longevity
mot
Metabolic
MOTS-c
COMPATIBLE
Both affect mitochondrial function but through different mechanisms.
Side effects
Contraindications
Hyperthyroidism or thyroid disorders
Heart conditions
Pregnancy or breastfeeding
Use of other mitochondrial uncouplers (DNP, FCCP)
Fever or active infection
Stop signs
Significant hyperthermia/overheating
Excessive sweating
Rapid heart rate
Difficulty breathing
Bad signs
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Frequently asked questions
What is a peptide dosage calculator?
A peptide dosage calculator is a free tool that converts your vial size, bacteriostatic water volume, and target dose into an exact syringe draw volume. Instead of doing the reconstitution math by hand, you enter three inputs and instantly get the concentration of your solution and how many milliliters or syringeunits to draw. This calculator works for single peptide compounds and multi-peptide blends.
How do I calculate peptide dosage from a vial?
To calculate your peptide dose, divide the total peptide content of your vial in micrograms by the volume of bacteriostatic water you added in milliliters. This gives you your solution concentration in mcg/mL. Then divide your target dose by that concentration to get your draw volume. For example, a 5mg (5,000 mcg) vial reconstituted with 2mL of BAC water gives a concentration of 2,500 mcg/mL. A 250 mcg dose would require drawing 0.1mL. This calculator automates all of those steps instantly.
How much Bacteriostatic water should I add to a peptide vial?
Most people add 2mL to 3mL of bacteriostatic water per vial, but the right amount depends on the dose you want to draw and the syringe size you are using. Adding 1mL to a 5mg vial gives you a concentration of 5,000 mcg/mL, making each dose very small in volume. Adding 2mL gives you 2,500 mcg/mL, which is easier to measure on a standard insulin syringe. A general guideline is to choose a volume that puts your typical dose somewhere between 10 and 30 units on a U-100 syringe. Use the calculator above to test different water volumes and find what works for your dose.
How are peptides different from proteins?
Both are made of amino acids, but peptides are much smaller than proteins. Because of their tiny size, peptides can act like tiny messengers in the body, sending specific signals to your cells to tell them exactly what to do.
BAM-15Research References
BAM-15 is a preclinical compound
BAM-15
BAM-15 is a preclinical compound
Mitochondrial uncoupler BAM15 reverses diet-induced obesity and insulin resistance in mice
BAM15 is orally bioavailable, increases nutrient oxidation, decreases body fat mass without altering food intake, lean mass, body temperature, or markers of toxicity.
n.d.
BAM15-mediated mitochondrial uncoupling protects against obesity and improves glycemic control
BAM15 is 7-fold more potent than DNP, does not depolarize plasma membranes, and does not induce apoptosis at effective doses.
n.d.
BAM15 as a mitochondrial uncoupler: a promising therapeutic agent for diverse diseases
Comprehensive review of BAM15 mechanisms including AMPK and PGC-1α activation pathways.
n.d.
Beneficial effects of simultaneously targeting calorie intake and calorie efficiency in diet-induced obese mice
Combining semaglutide and BAM15 produces stronger metabolic benefits than either alone; helps overcome metabolic adaptation.
n.d.
Calculate peptide dosages
Learning how to calculate a peptide dose? Use our beginner-friendly peptide dosage, blend, and accumulation calculators. Enter vial size, reconstitution volume, and target dose to get exact draw volumes instantly. No guesswork, just clear guidance that helps prevent common mistakes.
