Weight
AOD-9604
Metabolic
16
Amino acids
Molecular weight
Peptide
Type
AOD-9604 is a modified fragment of human growth hormone (amino acids 176-191) that stimulates lipolysis and inhibits lipogenesis without the side effects of full growth hormone. Unlike full GH, it does not increase IGF-1, affect glucose metabolism, or cause insulin resistance.
Top researched benefits
Overview of AOD-9604
Stimulates lipolysis and inhibits lipogenesis through beta-3 adrenergic receptor upregulation, without binding to the GH receptor. Does not affect glucose metabolism or increase IGF-1 levels.
fat loss
- Increases fat oxidation, plasma glycerol levels, and reduces body weight in obese models without affecting glucose metabolism.
- Reduces fat synthesis and storage in adipose tissue.
- Does not cause hyperglycemia, reduce insulin secretion, or increase insulin resistance.
joint health
- Enhanced cartilage regeneration when combined with hyaluronic acid in osteoarthritis models.
- Synergistic effects with hyaluronic acid for joint health.
Typical Dose
250-500mcg
Frequency
Once daily
Cycle Duration
8-12 weeks
Storage
Lyophilized: room temp or freezer long-term. Reconstituted: 2-8°C for 28 days
Chemical Makeup
Key benefits
Targets fat loss through selective lipolysis
Avoids growth hormone side effects
No effect on blood glucose or insulin
Does not increase IGF-1 levels
Metabolically safer than full hGH
Emerging cartilage repair potential
Community interest
This peptide is still gaining traction in the community.
Modified hGH Fragment | Fat Loss Peptide
This overview is informational and based on aggregated descriptions from studies and user reports.
Was it helpful?YesNoAOD-9604 Molecular Information
View the scientifc details of AOD-9604.
16
Amino Acids
AOD-9604
Tyr
Tyr
Position 1
Leu
Leu
Position 2
Arg
Arg
Position 3
Ile
Ile
Position 4
Val
Val
Position 5
Gln
Gln
Position 6
Cys
Cys
Position 7
Arg
Arg
Position 8
Ser
Ser
Position 9
Val
Val
Position 10
Glu
Glu
Position 11
Gly
Gly
Position 12
Ser
Ser
Position 13
Cys
Cys
Position 14
Gly
Gly
Position 15
Phe
Phe
Position 16
Molecular Weight
1815.1DaChain Length
16Amino AcidsType
PeptideAOD-9604 Protocols
Subcutaneous injection is the standard route. Administer in the morning on an empty stomach, wait 30 minutes before eating for optimal results.
| Goal | Dosage | Frequency | Route |
|---|---|---|---|
| Fat loss - Standard | 250 | 1 week range | SubQ |
| Enhanced fat loss | 400 | 1 week range | SubQ |
| Joint support | 250 | 1 week range | SubQ |
| Conservative start | 200 | 1 week range | SubQ |
Reconstitution Instructions
Materials needed:
Steps to reconstitute
- Allow vial to reach room temperature (15-20 minutes)
- Calculate required bacteriostatic water volume
- Clean vial stopper with alcohol swab
- Draw BAC water into syringe
- Inject slowly down vial side (not directly onto powder)
- Gently swirl until dissolved (never shake vigorously)
- Store reconstituted solution at 2-8°C
- Use within 28 days
AOD-9604 Cycle
The AOD-9604 Cycle section explains how long a typical cycle lasts and what to expect during each phase. Over time, your body can become less responsive with continuous use.
Taking breaks between cycles may help maintain effectiveness and support better overall results.
- Week 1-2
- Minimal noticeable effects; compound accumulating
- Week 3-4
- Subtle body composition changes may begin
- Week 5-8
- More noticeable fat loss, especially with diet and exercise
- Week 8-12
- Continued fat reduction and improved body composition
Dosing tools
AOD-9604 Peptide Dosage Calculator
Calculate peptide doses with our visual syringe guide.
mg
Enter the total amount of peptide in the vial in milligrams (as stated on the label).
The dose you want to inject per administration, in mcg or mg.
1,000 mcg = 1 mg
1 mL
2 mL
3 mL
5 mL
Custom
Volume of bacteriostatic water you add to reconstitute the powder. Use BAC water for preservation.
Injection Results
Based on your vial and dilution inputs.
AOD-9604
SINGLE COMPOUNDVolume per injection
0.05
mL
Concentration
10.00
mg/mL
Doses per vial
20
doses
Total injections per vial
20 injections
How it works
Based on a 10 mg AOD-9604 vial diluted with 1 mL of bacteriostatic water, each 500 mcg injection equals 0.05 mL.
1mL / 100 units
5 units
0.050 mL
Reference Guide
Dosing Cycle
- Peptide
- AOD-9604
- Dosing
- 250-500mcg
- Dosing Frequency
- Once daily
- Cycle Duration
- 8-12 weeks
- Storage
- Lyophilized: room temp or freezer long-term. Reconstituted: 2-8°C for 28 days
Note: Triple agonist; microdose for fewer side effects
Reconstitution Tips
- Use bacteriostatic water (BAC) — contains 0.9% benzyl alcohol for preservation
- Inject water slowly — aim down the vial wall, not directly onto powder
- Never shake — gently swirl or roll the vial until dissolved
- Store properly — refrigerate at 2-8°C after reconstitution
- Use within 28 days — most reconstituted peptides remain stable for about 4 weeks
- Keep sterile — always clean vial tops with alcohol before drawing
Peptide Interactions
Research suggestions of AOD-9604 interactions with other common peptides and substances.
Healing
bpc
Longevity
BPC-157
COMPATIBLE
Different mechanisms; sometimes combined for comprehensive healing.
Weight
sem
Metabolic
Semaglutide
COMPATIBLE
Complementary weight loss mechanisms through different pathways.
Side effects
Contraindications
Pregnancy or breastfeeding
WADA prohibited - athletes subject to testing must avoid
Stop signs
Severe injection site reactions or infection signs
Unusual swelling or persistent redness
Allergic reactions (rare)
Any concerning or unexpected symptoms
Bad signs
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Frequently asked questions
What is a peptide dosage calculator?
A peptide dosage calculator is a free tool that converts your vial size, bacteriostatic water volume, and target dose into an exact syringe draw volume. Instead of doing the reconstitution math by hand, you enter three inputs and instantly get the concentration of your solution and how many milliliters or syringeunits to draw. This calculator works for single peptide compounds and multi-peptide blends.
How do I calculate peptide dosage from a vial?
To calculate your peptide dose, divide the total peptide content of your vial in micrograms by the volume of bacteriostatic water you added in milliliters. This gives you your solution concentration in mcg/mL. Then divide your target dose by that concentration to get your draw volume. For example, a 5mg (5,000 mcg) vial reconstituted with 2mL of BAC water gives a concentration of 2,500 mcg/mL. A 250 mcg dose would require drawing 0.1mL. This calculator automates all of those steps instantly.
How much Bacteriostatic water should I add to a peptide vial?
Most people add 2mL to 3mL of bacteriostatic water per vial, but the right amount depends on the dose you want to draw and the syringe size you are using. Adding 1mL to a 5mg vial gives you a concentration of 5,000 mcg/mL, making each dose very small in volume. Adding 2mL gives you 2,500 mcg/mL, which is easier to measure on a standard insulin syringe. A general guideline is to choose a volume that puts your typical dose somewhere between 10 and 30 units on a U-100 syringe. Use the calculator above to test different water volumes and find what works for your dose.
How are peptides different from proteins?
Both are made of amino acids, but peptides are much smaller than proteins. Because of their tiny size, peptides can act like tiny messengers in the body, sending specific signals to your cells to tell them exactly what to do.
AOD-9604Research References
AOD-9604 is a well studied compound
AOD-9604
AOD-9604 is a well studied compound
Fat Oxidation and Weight Loss in Obese Mice - Heffernan et al.
AOD-9604 reduced body weight without hyperglycemia through enhanced fat oxidation.
n.d.
Beta-3 Adrenergic Receptor Mechanism - Heffernan et al.
Increased beta-3 adrenergic receptor expression comparable to lean mice.
n.d.
Oral Lipid Metabolism Effects - Heffernan et al.
Significantly reduced lipogenic activity and increased lipolytic activity.
n.d.
Cartilage Regeneration with Hyaluronic Acid - Kwon & Park
Combination accelerated cartilage regeneration in osteoarthritis models.
n.d.