Longevity
Epitalon
Immunity
4
Amino acids
Molecular weight
Peptide
Type
Epitalon is a synthetic tetrapeptide (Ala-Glu-Asp-Gly) derived from the bovine pineal gland extract epithalamin, studied primarily for its geroprotective and anti-aging properties. It activates telomerase enzyme activity, promoting telomere elongation in human somatic cells and extending replicative lifespan beyond the Hayflick limit. It is used in longevity research and by biohackers seeking cellular rejuvenation, circadian rhythm restoration, and lifespan extension.
Top researched benefits
Overview of Epitalon
Epitalon upregulates telomerase reverse transcriptase (TERT) activity, elongating telomeres in aging somatic cells to delay replicative senescence. It also directly influences melatonin synthesis in pineal cells via AANAT and pCREB modulation, reduces oxidative stress by enhancing superoxide dismutase and glutathione peroxidase activity, and binds specific DNA sequences (ATTTC and CAG regions) to exert epigenetic regulatory effects.
antiaging
- Activates telomerase at microgram doses to extend telomeres and cellular replicative lifespan.
- Reverses aging markers and improves mitochondrial function at the cellular level.
- Animal studies show 25-33% lifespan increases using 30-40mcg/kg doses.
neuroprotection
- Restores pineal gland function and circadian melatonin rhythm in elderly.
- Supports new neuron formation at nanogram concentrations.
- 90% response rate for retinal degeneration at 5mcg per eye dosing.
immunity
- Supports thymus gland regeneration for improved immune function.
- Enhanced immune surveillance and countering of immunosenescence.
Typical Dose
10mg per injection
Frequency
once daily for 10 consecutive days
Cycle Duration
10-day course repeated every 4-6 months
Storage
Lyophilized: store at -20°C long-term, or 2-8°C short-term, protected from moisture. Reconstituted: refrigerate at 2-8°C and use within 4-6 weeks.
Chemical Makeup
Key benefits
Activates telomerase and elongates telomeres in human somatic cells, extending replicative lifespan beyond the Hayflick limit
Restores melatonin secretion by the pineal gland in aged organisms, improving circadian rhythm and sleep quality
Reduces spontaneous tumor incidence and chromosomal aberrations in animal models, suggesting antimutagenic and anticancer properties
Enhances antioxidant enzyme activity (superoxide dismutase, glutathione peroxidase) to reduce oxidative stress and lipid peroxidation in aging tissue
Community interest
This peptide is still gaining traction in the community.
Telomerase Activator | Longevity & Anti-Aging
This overview is informational and based on aggregated descriptions from studies and user reports.
Was it helpful?YesNoEpitalon Molecular Information
View the scientifc details of Epitalon.
4
Amino Acids
Epitalon
Ala
Ala
Position 1
Glu
Glu
Position 2
Asp
Asp
Position 3
Gly
Gly
Position 4
Molecular Weight
390.349DaChain Length
4Amino AcidsType
PeptideEpitalon Protocols
Subcutaneous or intramuscular injection is the best-documented and most bioavailable delivery route for Epitalon, with the majority of human and animal research conducted using this method. Most protocols administer SubQ in the abdomen each evening during a defined 10–20 day cycle, repeated once or twice per year with a 4–6 month off-cycle interval.
| Goal | Dosage | Frequency | Route |
|---|---|---|---|
| Activate telomerase, extend telomere length, and slow cellular senescence over a 20-day course repeated 1–2 times per year | 5 | 1 day range | SubQ |
| Achieve equivalent total cycle dose (100 mg) in a compressed 10-day window for users who prefer shorter administration periods | 10 | 1 day range | SubQ |
| Induce telomerase enzyme expression in somatic cells to measurably elongate telomeres and reduce biological age markers | 10 | 1 day range | SubQ or IM |
| Restore age-related decline in pineal melatonin secretion and re-establish normal circadian rhythm function | 5 | 1 day range | SubQ (evening) |
| Recalibrate immune function and reduce systemic oxidative stress markers associated with biological aging | 10 | 1 day range | SubQ or IM |
Reconstitution Instructions
Materials needed:
Steps to reconstitute
- Draw 2 mL of bacteriostatic water into a sterile 3–5 mL reconstitution syringe
- Insert the needle into the Epitalon vial at an angle and inject the BAC water slowly down the inside wall of the vial — do not aim directly onto the powder
- Remove the syringe and gently swirl the vial until the powder is fully dissolved — do not shake
- Label the vial with the reconstitution date and store in the refrigerator at 2–8 °C (35.6–46.4 °F), protected from light
- Use the reconstituted solution within 28 days; discard if it appears cloudy or contains visible particles
- To draw a dose, insert a fresh insulin syringe into the vial and withdraw the calculated volume corresponding to your target dose
- Pinch a small fold of skin on the abdomen, insert the needle at a 45-degree angle, and inject slowly; rotate sites across the abdomen, thighs, and upper arms each day to avoid tissue buildup
Epitalon Cycle
The Epitalon Cycle section explains how long a typical cycle lasts and what to expect during each phase. Over time, your body can become less responsive with continuous use.
Taking breaks between cycles may help maintain effectiveness and support better overall results.
- Day 1-3
- Subtle energy improvement possible
- Day 3-7
- Better sleep quality, vivid dreams (melatonin effect)
- Week 0-0
- Enhanced morning energy, mood stability
- Week 3-4
- Skin quality improvements, enhanced wound healing
- Month 2-3
- Laboratory markers may show telomere changes
- Week 0-0
- Cumulative anti-aging benefits with repeated cycles
Dosing tools
Epitalon Peptide Dosage Calculator
Calculate peptide doses with our visual syringe guide.
mg
Enter the total amount of peptide in the vial in milligrams (as stated on the label).
The dose you want to inject per administration, in mcg or mg.
1,000 mcg = 1 mg
1 mL
2 mL
3 mL
5 mL
Custom
Volume of bacteriostatic water you add to reconstitute the powder. Use BAC water for preservation.
Injection Results
Based on your vial and dilution inputs.
Epitalon
SINGLE COMPOUNDVolume per injection
0.05
mL
Concentration
10.00
mg/mL
Doses per vial
20
doses
Total injections per vial
20 injections
How it works
Based on a 10 mg Epitalon vial diluted with 1 mL of bacteriostatic water, each 500 mcg injection equals 0.05 mL.
1mL / 100 units
5 units
0.050 mL
Reference Guide
Dosing Cycle
- Peptide
- Epitalon
- Dosing
- 10mg per injection
- Dosing Frequency
- once daily for 10 consecutive days
- Cycle Duration
- 10-day course repeated every 4-6 months
- Storage
- Lyophilized: store at -20°C long-term, or 2-8°C short-term, protected from moisture. Reconstituted: refrigerate at 2-8°C and use within 4-6 weeks.
Note: Triple agonist; microdose for fewer side effects
Reconstitution Tips
- Use bacteriostatic water (BAC) — contains 0.9% benzyl alcohol for preservation
- Inject water slowly — aim down the vial wall, not directly onto powder
- Never shake — gently swirl or roll the vial until dissolved
- Store properly — refrigerate at 2-8°C after reconstitution
- Use within 28 days — most reconstituted peptides remain stable for about 4 weeks
- Keep sterile — always clean vial tops with alcohol before drawing
Peptide Interactions
Research suggestions of Epitalon interactions with other common peptides and substances.
Longevity
thy
Immunity
Thymalin
SYNERGISTIC
Enhanced immunomodulation - commonly combined in Russian protocols.
Side effects
Contraindications
Active malignancy (telomerase activation concern)
Chronic sinusitis (nasal route)
Known peptide allergies
Pregnancy or breastfeeding
Stop signs
Signs of allergic reaction
Persistent injection site inflammation
New or changing moles (monitor skin)
Cancer diagnosis (discontinue immediately)
Unusual fatigue or weakness
Bad signs
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Frequently asked questions
What is a peptide dosage calculator?
A peptide dosage calculator is a free tool that converts your vial size, bacteriostatic water volume, and target dose into an exact syringe draw volume. Instead of doing the reconstitution math by hand, you enter three inputs and instantly get the concentration of your solution and how many milliliters or syringeunits to draw. This calculator works for single peptide compounds and multi-peptide blends.
How do I calculate peptide dosage from a vial?
To calculate your peptide dose, divide the total peptide content of your vial in micrograms by the volume of bacteriostatic water you added in milliliters. This gives you your solution concentration in mcg/mL. Then divide your target dose by that concentration to get your draw volume. For example, a 5mg (5,000 mcg) vial reconstituted with 2mL of BAC water gives a concentration of 2,500 mcg/mL. A 250 mcg dose would require drawing 0.1mL. This calculator automates all of those steps instantly.
How much Bacteriostatic water should I add to a peptide vial?
Most people add 2mL to 3mL of bacteriostatic water per vial, but the right amount depends on the dose you want to draw and the syringe size you are using. Adding 1mL to a 5mg vial gives you a concentration of 5,000 mcg/mL, making each dose very small in volume. Adding 2mL gives you 2,500 mcg/mL, which is easier to measure on a standard insulin syringe. A general guideline is to choose a volume that puts your typical dose somewhere between 10 and 30 units on a U-100 syringe. Use the calculator above to test different water volumes and find what works for your dose.
How are peptides different from proteins?
Both are made of amino acids, but peptides are much smaller than proteins. Because of their tiny size, peptides can act like tiny messengers in the body, sending specific signals to your cells to tell them exactly what to do.
EpitalonResearch References
Epitalon is a preclinical compound
Epitalon
Epitalon is a preclinical compound
Telomerase Activation Study
Human fibroblasts showed extended replicative lifespan (34→44+ passages) at 0.1μM concentration while maintaining telomere length comparable to young cells.
2003
Russian Clinical Trials
Long-term observations showed significantly lower all-cause mortality in elderly subjects receiving periodic epithalamin courses with improved cardiovascular and immune parameters.
1992
Pineal Function Study
Elderly patients showed restoration of circadian melatonin rhythm and improved sleep/emotional stability using low-dose synthetic treatment.
2001
Retinal Degeneration Study
AMD patients achieved 90% positive response with only 5mcg per eye daily dosing.
2019
Lifespan Extension Study
Aging mice using 30-40mcg/kg showed 25% lifespan increase and reduced chromosomal aberrations.
2003
Neurogenesis Study
Human stem cells showed 1.6-1.8 fold upregulation of neurogenic markers at extremely low concentrations (0.01mcg/mL).
2020
Calculate peptide dosages
Learning how to calculate a peptide dose? Use our beginner-friendly peptide dosage, blend, and accumulation calculators. Enter vial size, reconstitution volume, and target dose to get exact draw volumes instantly. No guesswork, just clear guidance that helps prevent common mistakes.
