Ovagen
FDA APPROVAL PENDING
Experimental
Longevity
Ovagen
3
Amino acids
Molecular weight
Peptide
Type
Ovagen is a synthetic tripeptide bioregulator composed of glutamic acid, aspartic acid, and leucine (Glu-Asp-Leu), developed through Russian peptide research to target liver and gastrointestinal tissue. It works by penetrating cell nuclei and modulating DNA methylation, restoring gene expression patterns in hepatic and GI tissue to a more youthful state. It is used primarily in longevity, liver health, and recovery contexts, particularly among older populations and those with liver stress from toxins or chemotherapy.
Top researched benefits
Overview of Ovagen
Ovagen enters cells via PEPT1/PEPT2 peptide transporter proteins and penetrates the nucleus, where it modulates DNA methylation and chromatin condensation to normalize gene transcription in liver and gastrointestinal tissue. This epigenetic mechanism reverses age-related silencing of repair genes, enabling hepatic cells to produce enzymes and proteins needed for regeneration and fibrosis prevention.
liver support
- Reduces long-term fibrosis development in liver tissue.
- Protects liver through gene expression regulation.
gi protection
- Helps protect GI mucosal layer from various insults.
- May protect GI tract from antibiotic-induced damage.
- Protects against environmental toxin effects on GI tract.
antiaging
- Addresses age-related liver changes through bioregulation.
- Supports GI tract function during aging.
Typical Dose
10mg per day (up to 20mg in therapeutic protocols)
Frequency
once daily
Cycle Duration
10–20 day course (midpoint ~14 days), repeated 2–3 times per year
Storage
Lyophilized powder: store at -20°C, protected from light, stable for 24+ months. Reconstituted: refrigerate at 2-8°C and use within 2-4 weeks. Capsule form: room temperature.
Chemical Makeup
Key benefits
Reduces hepatic fibrosis by inhibiting fibrotic gene expression and restoring DNA methylation patterns in liver fibroblasts
Supports liver regeneration after hepatitis, chemotherapy, or antibiotic-induced stress by activating repair-associated gene transcription
Strengthens gastrointestinal mucosal barrier integrity, reducing gut permeability and susceptibility to toxin-induced enteropathy
Reverses age-related DNA condensation in hepatic cells, making repair genes more accessible and functionally active in older individuals
Community interest
This peptide is still gaining traction in the community.
Liver & GI Bioregulator | Epigenetic DNA Repair
This overview is informational and based on aggregated descriptions from studies and user reports.
Was it helpful?YesNoOvagen Molecular Information
View the scientifc details of Ovagen.
3
Amino Acids
Ovagen
Glu
Glu
Position 1
Asp
Asp
Position 2
Leu
Leu
Position 3
Molecular Weight
375.37DaChain Length
3Amino AcidsType
PeptideOvagen Protocols
Subcutaneous injection is the primary research-grade delivery route for Ovagen, a tripeptide (Glu-Asp-Leu, MW 375 Da) bioregulator targeting liver and gastrointestinal tissue. Injection into abdominal fat or thigh subcutaneous tissue is preferred, typically administered once daily before bed following a slow dose-escalation schedule.
| Goal | Dosage | Frequency | Route |
|---|---|---|---|
| Liver support — dose escalation (weeks 1–2) | 10 | 1 day range | SubQ |
| Liver support — dose escalation (weeks 3–4) | 20 | 1 day range | SubQ |
| Liver support — dose escalation (weeks 5–6) | 50 | 1 day range | SubQ |
| Liver support — dose escalation (weeks 7–8) | 100 | 1 day range | SubQ |
| Liver support — maintenance (weeks 9–16) | 150 | 1 day range | SubQ |
| Longevity / anti-aging stack | 20 | 1 day range | SubQ |
Reconstitution Instructions
Materials needed:
Steps to reconstitute
- Allow the lyophilized Ovagen vial to equilibrate to room temperature for 15–20 minutes before reconstitution
- Draw 2.0 mL of bacteriostatic water into an insulin syringe
- Inject the bacteriostatic water slowly down the inner glass wall of the vial — never directly onto the lyophilized powder
- Gently swirl the vial until the powder is fully dissolved — do not shake
- The reconstituted solution yields 10 mg/mL (100 mcg per 0.01 mL on a U-100 syringe); store at 2–8°C and use within 2–4 weeks
- Clean the injection site with an alcohol swab and allow to dry
- Pinch a skinfold and insert the insulin syringe needle at a 45–90° angle into subcutaneous tissue
- Inject slowly and steadily without aspirating; withdraw needle and apply light pressure
- Rotate injection sites (abdomen, thighs, upper arms) keeping at least 2 cm between sites to avoid lipohypertrophy
Ovagen Cycle
The Ovagen Cycle section explains how long a typical cycle lasts and what to expect during each phase. Over time, your body can become less responsive with continuous use.
Taking breaks between cycles may help maintain effectiveness and support better overall results.
- Week 0-0
- Gene expression modulation begins
- Week 0-0
- Effects persist due to epigenetic changes
- Week 0-0
- Liver and GI function improvements
- Week 0-0
- Cumulative benefits with periodic cycles
Dosing tools
Ovagen Peptide Dosage Calculator
Calculate peptide doses with our visual syringe guide.
mg
Enter the total amount of peptide in the vial in milligrams (as stated on the label).
The dose you want to inject per administration, in mcg or mg.
1,000 mcg = 1 mg
1 mL
2 mL
3 mL
5 mL
Custom
Volume of bacteriostatic water you add to reconstitute the powder. Use BAC water for preservation.
Injection Results
Based on your vial and dilution inputs.
Ovagen
SINGLE COMPOUNDVolume per injection
0.05
mL
Concentration
10.00
mg/mL
Doses per vial
20
doses
Total injections per vial
20 injections
How it works
Based on a 10 mg Ovagen vial diluted with 1 mL of bacteriostatic water, each 500 mcg injection equals 0.05 mL.
1mL / 100 units
5 units
0.050 mL
Reference Guide
Dosing Cycle
- Peptide
- Ovagen
- Dosing
- 10mg per day (up to 20mg in therapeutic protocols)
- Dosing Frequency
- once daily
- Cycle Duration
- 10–20 day course (midpoint ~14 days), repeated 2–3 times per year
- Storage
- Lyophilized powder: store at -20°C, protected from light, stable for 24+ months. Reconstituted: refrigerate at 2-8°C and use within 2-4 weeks. Capsule form: room temperature.
Note: Triple agonist; microdose for fewer side effects
Reconstitution Tips
- Use bacteriostatic water (BAC) — contains 0.9% benzyl alcohol for preservation
- Inject water slowly — aim down the vial wall, not directly onto powder
- Never shake — gently swirl or roll the vial until dissolved
- Store properly — refrigerate at 2-8°C after reconstitution
- Use within 28 days — most reconstituted peptides remain stable for about 4 weeks
- Keep sterile — always clean vial tops with alcohol before drawing
Peptide Interactions
Research suggestions of Ovagen interactions with other common peptides and substances.
Longevity
epi
Immunity
Epitalon
SYNERGISTIC
Often combined in comprehensive anti-aging Khavinson protocols.
Longevity
glu
Immunity
Glutathione
SYNERGISTIC
Both support liver health through different pathways.
Longevity
liv
Immunity
Livagen
SYNERGISTIC
Complementary liver bioregulators with different sequences and mechanisms.
Side effects
Contraindications
Active liver emergencies (seek medical care)
Known hypersensitivity
Pregnancy or breastfeeding
Stop signs
Allergic reactions
Unusual GI or liver symptoms
Bad signs
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Frequently asked questions
What is a peptide dosage calculator?
A peptide dosage calculator is a free tool that converts your vial size, bacteriostatic water volume, and target dose into an exact syringe draw volume. Instead of doing the reconstitution math by hand, you enter three inputs and instantly get the concentration of your solution and how many milliliters or syringeunits to draw. This calculator works for single peptide compounds and multi-peptide blends.
How do I calculate peptide dosage from a vial?
To calculate your peptide dose, divide the total peptide content of your vial in micrograms by the volume of bacteriostatic water you added in milliliters. This gives you your solution concentration in mcg/mL. Then divide your target dose by that concentration to get your draw volume. For example, a 5mg (5,000 mcg) vial reconstituted with 2mL of BAC water gives a concentration of 2,500 mcg/mL. A 250 mcg dose would require drawing 0.1mL. This calculator automates all of those steps instantly.
How much Bacteriostatic water should I add to a peptide vial?
Most people add 2mL to 3mL of bacteriostatic water per vial, but the right amount depends on the dose you want to draw and the syringe size you are using. Adding 1mL to a 5mg vial gives you a concentration of 5,000 mcg/mL, making each dose very small in volume. Adding 2mL gives you 2,500 mcg/mL, which is easier to measure on a standard insulin syringe. A general guideline is to choose a volume that puts your typical dose somewhere between 10 and 30 units on a U-100 syringe. Use the calculator above to test different water volumes and find what works for your dose.
How are peptides different from proteins?
Both are made of amino acids, but peptides are much smaller than proteins. Because of their tiny size, peptides can act like tiny messengers in the body, sending specific signals to your cells to tell them exactly what to do.
OvagenResearch References
Ovagen is an experimental compound
Ovagen
Ovagen is an experimental compound
Khavinson Peptide Bioregulators Overview
Ovagen (EDL) is a tripeptide bioregulator with effects on liver and GI tract.
n.d.
POT Family Transporters and Peptide Bioregulators
Di- and tripeptides are transported via PEPT1/PEPT2, explaining tissue-specific targeting.
n.d.
Peptide Bioregulators and Liver Fibrosis
Short peptides reduce long-term fibrosis in liver tissue through gene expression regulation.
n.d.
Calculate peptide dosages
Learning how to calculate a peptide dose? Use our beginner-friendly peptide dosage, blend, and accumulation calculators. Enter vial size, reconstitution volume, and target dose to get exact draw volumes instantly. No guesswork, just clear guidance that helps prevent common mistakes.
