Sexual
Kisspeptin
Hormonal
9
Amino acids
Molecular weight
Peptide
Type
Kisspeptin is a naturally occurring neuropeptide encoded by the KISS1 gene that acts as the master upstream regulator of the hypothalamic-pituitary-gonadal (HPG) axis. It binds to the KISS1 receptor (GPR54) on GnRH neurons to trigger pulsatile GnRH release, which drives LH and FSH secretion and downstream sex steroid production. It is studied in clinical research for treating reproductive disorders including hypothalamic amenorrhea, PCOS, male hypogonadism, and as a safer ovulation trigger in IVF protocols.
Top researched benefits
Overview of Kisspeptin
Kisspeptin binds selectively to the KISS1 receptor (GPR54), a Gq/11-coupled GPCR expressed on hypothalamic GnRH neurons, activating phospholipase C, intracellular calcium mobilization, and ERK1/2 signaling to stimulate pulsatile GnRH release. This upstream activation of the HPG axis drives pituitary secretion of LH and FSH, leading to gonadal sex steroid production in both males and females.
reproductive
- Restores reproductive hormone secretion in functional or congenital cases by stimulating dormant GnRH axis.
- Triggers oocyte maturation with 45% live birth rate and zero severe OHSS cases; safer than hCG.
- Restores reproductive hormone pulsatility in women with hypothalamic amenorrhea.
sexual function
- Modulates sexual brain processing; increases penile tumescence by 56% vs placebo.
- Modulates sexual and attraction brain processing; increases self-reported sexiness.
metabolic
- Preclinical evidence suggests influence on energy expenditure and activity levels.
Typical Dose
100–200mcg per injection (titrate up over first 2 weeks)
Frequency
once daily subcutaneous injection
Cycle Duration
8–12 weeks on, followed by a break
Storage
Lyophilized: -20°C in dark, dry conditions. Reconstituted with bacteriostatic water: 2–8°C for up to 4 weeks; avoid freeze-thaw cycles.
Chemical Makeup
Key benefits
Stimulates pulsatile GnRH release, restoring LH and FSH secretion in women with hypothalamic amenorrhea
Induces ovulation as a safer alternative to hCG triggers in IVF protocols, reducing risk of ovarian hyperstimulation syndrome (OHSS)
Rapidly elevates LH and testosterone levels in healthy men within hours of subcutaneous administration
Rescues ovulatory function in a subset of PCOS patients by reinstating hypothalamic GnRH pulsatility
Community interest
This peptide is still gaining traction in the community.
GnRH Upstream Activator | Reproductive & Hormonal Health
This overview is informational and based on aggregated descriptions from studies and user reports.
Was it helpful?YesNoKisspeptin Molecular Information
View the scientifc details of Kisspeptin.
9
Amino Acids
Kisspeptin
Asn
Asn
Position 1
Trp
Trp
Position 2
Asn
Asn
Position 3
Ser
Ser
Position 4
Phe
Phe
Position 5
Gly
Gly
Position 6
Leu
Leu
Position 7
Arg
Arg
Position 8
Phe
Phe
Position 9
Molecular Weight
1302.45DaChain Length
9Amino AcidsType
PeptideKisspeptin Protocols
Subcutaneous injection is the primary research route for Kisspeptin-10, delivering the peptide into fatty tissue to stimulate hypothalamic GnRH neurons and downstream LH and testosterone release. Morning administration between 7–9 AM is preferred to align with the natural cortisol rhythm and allow hormonal effects during waking hours.
| Goal | Dosage | Frequency | Route |
|---|---|---|---|
| Testosterone/LH stimulation (low dose) | 50 | 1 day range | SubQ |
| Testosterone/LH stimulation (standard) | 100 | 1 day range | SubQ |
| Testosterone/LH stimulation (advanced) | 200 | 1 day range | SubQ |
| PCT / Natural testosterone restart | 100 | 1 day range | SubQ |
| Testicular atrophy prevention (adjunct to TRT) | 150 | 2 week range | SubQ |
| Hypogonadotropic hypogonadism / fertility | 100 | 1 day range | SubQ |
Reconstitution Instructions
Materials needed:
Steps to reconstitute
- Draw 3.0 mL of bacteriostatic water into a sterile syringe
- Inject the bacteriostatic water slowly down the inside wall of the Kisspeptin-10 vial — never directly onto the powder
- Gently roll the vial between your palms until the powder is fully dissolved — do not shake, as shaking can denature the peptide
- Allow the reconstituted solution to sit for 1–2 minutes before drawing your dose; this creates a concentration of approximately 3.33 mg/mL (33.3 mcg per unit on a U-100 syringe)
- Store the reconstituted vial in the refrigerator at 2–8 °C; use within 4 weeks and avoid freeze-thaw cycles
- Clean the injection site with an alcohol swab and allow it to dry fully before injecting
- Pinch a skinfold at the chosen site and insert the insulin needle at a 45–90° angle into the subcutaneous tissue
- Inject slowly and steadily without aspirating; withdraw the needle and apply gentle pressure with a clean swab
- Rotate injection sites systematically across the abdomen (at least 2 inches from the navel), outer thighs, upper arms, and upper buttocks to prevent tissue irritation
- Dispose of the used syringe immediately in a proper sharps container
Kisspeptin Cycle
The Kisspeptin Cycle section explains how long a typical cycle lasts and what to expect during each phase. Over time, your body can become less responsive with continuous use.
Taking breaks between cycles may help maintain effectiveness and support better overall results.
- Week 0-0
- LH surge peaks at 3-5x baseline
- Week 0-0
- LH returns to baseline
- Day 1-2
- Testosterone/estradiol increase in response to LH
- Week 2-4
- Potential improvements in sexual function and libido
Dosing tools
Kisspeptin Peptide Dosage Calculator
Calculate peptide doses with our visual syringe guide.
mg
Enter the total amount of peptide in the vial in milligrams (as stated on the label).
The dose you want to inject per administration, in mcg or mg.
1,000 mcg = 1 mg
1 mL
2 mL
3 mL
5 mL
Custom
Volume of bacteriostatic water you add to reconstitute the powder. Use BAC water for preservation.
Injection Results
Based on your vial and dilution inputs.
Kisspeptin
SINGLE COMPOUNDVolume per injection
0.05
mL
Concentration
10.00
mg/mL
Doses per vial
20
doses
Total injections per vial
20 injections
How it works
Based on a 10 mg Kisspeptin vial diluted with 1 mL of bacteriostatic water, each 500 mcg injection equals 0.05 mL.
1mL / 100 units
5 units
0.050 mL
Reference Guide
Dosing Cycle
- Peptide
- Kisspeptin
- Dosing
- 100–200mcg per injection (titrate up over first 2 weeks)
- Dosing Frequency
- once daily subcutaneous injection
- Cycle Duration
- 8–12 weeks on, followed by a break
- Storage
- Lyophilized: -20°C in dark, dry conditions. Reconstituted with bacteriostatic water: 2–8°C for up to 4 weeks; avoid freeze-thaw cycles.
Note: Triple agonist; microdose for fewer side effects
Reconstitution Tips
- Use bacteriostatic water (BAC) — contains 0.9% benzyl alcohol for preservation
- Inject water slowly — aim down the vial wall, not directly onto powder
- Never shake — gently swirl or roll the vial until dissolved
- Store properly — refrigerate at 2-8°C after reconstitution
- Use within 28 days — most reconstituted peptides remain stable for about 4 weeks
- Keep sterile — always clean vial tops with alcohol before drawing
Peptide Interactions
Research suggestions of Kisspeptin interactions with other common peptides and substances.
Sexual
hcg
Hormonal
HCG
COMPATIBLE
Serves as potentially safer alternative for IVF ovulation triggering.
Side effects
Contraindications
Not recommended during pregnancy or breastfeeding
Caution with cardiovascular disease history
Daily dosing causes receptor desensitization
Stop signs
Ovarian hyperstimulation signs: severe pelvic pain, bloating, nausea
Chest pain or cardiovascular symptoms
Severe headaches or visual disturbances
Persistent injection site reactions
Bad signs
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Frequently asked questions
What is a peptide dosage calculator?
A peptide dosage calculator is a free tool that converts your vial size, bacteriostatic water volume, and target dose into an exact syringe draw volume. Instead of doing the reconstitution math by hand, you enter three inputs and instantly get the concentration of your solution and how many milliliters or syringeunits to draw. This calculator works for single peptide compounds and multi-peptide blends.
How do I calculate peptide dosage from a vial?
To calculate your peptide dose, divide the total peptide content of your vial in micrograms by the volume of bacteriostatic water you added in milliliters. This gives you your solution concentration in mcg/mL. Then divide your target dose by that concentration to get your draw volume. For example, a 5mg (5,000 mcg) vial reconstituted with 2mL of BAC water gives a concentration of 2,500 mcg/mL. A 250 mcg dose would require drawing 0.1mL. This calculator automates all of those steps instantly.
How much Bacteriostatic water should I add to a peptide vial?
Most people add 2mL to 3mL of bacteriostatic water per vial, but the right amount depends on the dose you want to draw and the syringe size you are using. Adding 1mL to a 5mg vial gives you a concentration of 5,000 mcg/mL, making each dose very small in volume. Adding 2mL gives you 2,500 mcg/mL, which is easier to measure on a standard insulin syringe. A general guideline is to choose a volume that puts your typical dose somewhere between 10 and 30 units on a U-100 syringe. Use the calculator above to test different water volumes and find what works for your dose.
How are peptides different from proteins?
Both are made of amino acids, but peptides are much smaller than proteins. Because of their tiny size, peptides can act like tiny messengers in the body, sending specific signals to your cells to tell them exactly what to do.
KisspeptinResearch References
Kisspeptin is a phase 2 compound
Kisspeptin
Kisspeptin is a phase 2 compound
Kisspeptin for Hypoactive Sexual Desire Disorder in Men
Modulated sexual brain activity; increased penile tumescence by 56% vs placebo. JAMA Network Open.
2023
Kisspeptin for HSDD in Women
Modulated sexual and attraction processing; increased self-reported sexiness. JAMA Network Open.
2022
Kisspeptin as IVF Ovulation Trigger
45% live birth rate with zero severe OHSS; safer than hCG trigger. Journal of Clinical Investigation.
2017
Intranasal Kisspeptin Administration
Non-invasive delivery with rapid gonadotropin stimulation; stable 60-day formulation. eBioMedicine.
2025
Calculate peptide dosages
Learning how to calculate a peptide dose? Use our beginner-friendly peptide dosage, blend, and accumulation calculators. Enter vial size, reconstitution volume, and target dose to get exact draw volumes instantly. No guesswork, just clear guidance that helps prevent common mistakes.
