Longevity
Glutathione
Immunity
3
Amino acids
Molecular weight
Peptide
Type
Glutathione (GSH) is an endogenous tripeptide composed of glutamic acid, cysteine, and glycine, synthesized primarily in the liver and found in every cell of the body. It acts as the body's master antioxidant by directly scavenging reactive oxygen species, regenerating vitamins C and E, and conjugating toxins and heavy metals for hepatic excretion. Researchers, clinicians, and longevity-focused practitioners use exogenous glutathione via IV, IM, subcutaneous injection, or oral supplementation to combat oxidative stress, support liver detoxification, and slow cellular aging.
Top researched benefits
Overview of Glutathione
Glutathione exerts its effects through three primary pathways: direct ROS neutralization via the glutathione peroxidase-catalyzed reduction of hydrogen peroxide and lipid hydroperoxides to water; conjugation of electrophilic toxins and heavy metals via glutathione S-transferases to facilitate biliary and renal excretion; and maintenance of the intracellular redox state through the GSH/GSSG ratio, which regulates redox-sensitive transcription factors including Nrf2 and NF-κB.
antioxidant and detox
- Primary cellular antioxidant that neutralizes free radicals and protects against oxidative damage.
- Binds and facilitates elimination of heavy metals and environmental toxins.
- Essential for hepatic detoxification pathways and liver protection.
neurological
- IV glutathione showed improvements in mental function and speech quality in case studies.
- Protects neurons against oxidative damage and supports brain health.
immune and skin
- Improved Th1 cytokines and immune markers in HIV patients with supplementation.
- Supports skin health with potential brightening effects through melanin modulation.
- Combats age-related oxidative stress and supports cellular longevity.
Typical Dose
600mg per IV session (200–1400mg range)
Frequency
1–3 times per week
Cycle Duration
8 weeks on, reassess
Storage
Lyophilized powder: store at -20°C long-term or 2–8°C short-term (a few weeks). Reconstituted solution: 2–8°C, use within 1–2 weeks; do not refreeze.
Chemical Makeup
Key benefits
Neutralizes reactive oxygen species (superoxide, hydroxyl radicals, hydrogen peroxide) by donating electrons, protecting cellular macromolecules from oxidative damage
Facilitates hepatic Phase II detoxification by conjugating heavy metals, drugs, and environmental toxins via glutathione S-transferases, promoting biliary excretion
Regenerates oxidized vitamins C and E back to their active antioxidant forms, amplifying the overall antioxidant network
Supports mitochondrial function and ATP production by protecting mitochondrial membranes from lipid peroxidation and preserving electron transport chain integrity
Community interest
This peptide is still gaining traction in the community.
Master Antioxidant | Detoxification & Longevity
This overview is informational and based on aggregated descriptions from studies and user reports.
Was it helpful?YesNoGlutathione Molecular Information
View the scientifc details of Glutathione.
3
Amino Acids
Glutathione
?
?
Position 1
Cys
Cys
Position 2
Gly
Gly
Position 3
Molecular Weight
307.32DaChain Length
3Amino AcidsType
PeptideGlutathione Protocols
Subcutaneous injection is the standard self-administered route for Glutathione, delivering consistent antioxidant support at lower doses (100–200 mg/day) without the degradation seen with oral ingestion. Intravenous administration is reserved for higher-dose clinical protocols targeting skin brightening, chronic detoxification, or intensive antioxidant loading, where 100% bioavailability and rapid systemic distribution are required.
| Goal | Dosage | Frequency | Route |
|---|---|---|---|
| Establish a foundational antioxidant baseline and replenish intracellular glutathione stores depleted by daily oxidative stress | 100 | 1 day range | SubQ |
| Gradually increase systemic glutathione levels after a starter phase to improve redox balance and cellular protection without overwhelming clearance pathways | 200 | 1 day range | SubQ |
| Inhibit tyrosinase-driven melanin synthesis and shift melanogenesis from eumelanin to phaeomelanin for visible skin lightening; IV is the clinically studied route — the primary placebo-controlled trial used 1200 mg IV twice weekly for 6 weeks | 1200 | 2 week range | IV |
| Reduce exercise-induced oxidative stress, accelerate muscle recovery, and preserve mitochondrial function after high-intensity training | 800 | 2 week range | SubQ |
| Support Phase II hepatic glutathione conjugation of heavy metals and persistent organic toxins in chronically ill patients; IV route is preferred due to the higher tissue concentrations required for meaningful toxin binding and elimination | 1000 | 3 week range | IV |
| Achieve peak tissue saturation of glutathione for acute antioxidant support in high oxidative burden states; 1200–1500 mg IV is the commonly cited high-dose clinical range used by integrative clinics | 1400 | 2 week range | IV |
Reconstitution Instructions
Materials needed:
Steps to reconstitute
- Draw 2.0 mL of bacteriostatic water into an insulin syringe
- Insert the needle through the rubber stopper of the Glutathione vial at an angle
- Inject the bacteriostatic water slowly down the inner wall of the vial — do not aim directly at the powder
- Remove the syringe and gently swirl or roll the vial until the powder is fully dissolved — do not shake
- The resulting concentration is 300 mg/mL (for a 600 mg vial with 2.0 mL water)
- Label the vial with the date of reconstitution and store refrigerated at 2–8°C, protected from light
- Use the reconstituted solution within 2–3 weeks for optimal potency
- To inject, clean the injection site with an alcohol swab and allow it to dry
- Pinch the skin at the chosen site (abdomen, thigh, or upper arm), insert the needle at a 45-degree angle, and inject slowly
- Rotate injection sites with each dose to prevent tissue irritation
Glutathione Cycle
The Glutathione Cycle section explains how long a typical cycle lasts and what to expect during each phase. Over time, your body can become less responsive with continuous use.
Taking breaks between cycles may help maintain effectiveness and support better overall results.
- Week 0-0
- Antioxidant activity begins
- Week 0-0
- Improved energy and well-being may be noticed
- Week 2-4
- Cumulative detoxification benefits
- Week 4-12
- Full benefits for skin, immunity, and cellular health
Dosing tools
Glutathione Peptide Dosage Calculator
Calculate peptide doses with our visual syringe guide.
mg
Enter the total amount of peptide in the vial in milligrams (as stated on the label).
The dose you want to inject per administration, in mcg or mg.
1,000 mcg = 1 mg
1 mL
2 mL
3 mL
5 mL
Custom
Volume of bacteriostatic water you add to reconstitute the powder. Use BAC water for preservation.
Injection Results
Based on your vial and dilution inputs.
Glutathione
SINGLE COMPOUNDVolume per injection
0.05
mL
Concentration
10.00
mg/mL
Doses per vial
20
doses
Total injections per vial
20 injections
How it works
Based on a 10 mg Glutathione vial diluted with 1 mL of bacteriostatic water, each 500 mcg injection equals 0.05 mL.
1mL / 100 units
5 units
0.050 mL
Reference Guide
Dosing Cycle
- Peptide
- Glutathione
- Dosing
- 600mg per IV session (200–1400mg range)
- Dosing Frequency
- 1–3 times per week
- Cycle Duration
- 8 weeks on, reassess
- Storage
- Lyophilized powder: store at -20°C long-term or 2–8°C short-term (a few weeks). Reconstituted solution: 2–8°C, use within 1–2 weeks; do not refreeze.
Note: Triple agonist; microdose for fewer side effects
Reconstitution Tips
- Use bacteriostatic water (BAC) — contains 0.9% benzyl alcohol for preservation
- Inject water slowly — aim down the vial wall, not directly onto powder
- Never shake — gently swirl or roll the vial until dissolved
- Store properly — refrigerate at 2-8°C after reconstitution
- Use within 28 days — most reconstituted peptides remain stable for about 4 weeks
- Keep sterile — always clean vial tops with alcohol before drawing
Peptide Interactions
Research suggestions of Glutathione interactions with other common peptides and substances.
Longevity
nad
Metabolic
NAD+
SYNERGISTIC
Both support cellular energy and antioxidant systems; commonly combined in longevity protocols.
Longevity
ss3
Metabolic
SS-31
SYNERGISTIC
Both protect mitochondria; different mechanisms complement each other.
Side effects
Contraindications
Known allergy to glutathione
Pregnancy or breastfeeding (insufficient safety data)
Asthma (may trigger bronchospasm in some)
Stop signs
Signs of allergic reaction (rash, difficulty breathing)
Severe abdominal pain
Signs of zinc deficiency
Bad signs
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Frequently asked questions
What is a peptide dosage calculator?
A peptide dosage calculator is a free tool that converts your vial size, bacteriostatic water volume, and target dose into an exact syringe draw volume. Instead of doing the reconstitution math by hand, you enter three inputs and instantly get the concentration of your solution and how many milliliters or syringeunits to draw. This calculator works for single peptide compounds and multi-peptide blends.
How do I calculate peptide dosage from a vial?
To calculate your peptide dose, divide the total peptide content of your vial in micrograms by the volume of bacteriostatic water you added in milliliters. This gives you your solution concentration in mcg/mL. Then divide your target dose by that concentration to get your draw volume. For example, a 5mg (5,000 mcg) vial reconstituted with 2mL of BAC water gives a concentration of 2,500 mcg/mL. A 250 mcg dose would require drawing 0.1mL. This calculator automates all of those steps instantly.
How much Bacteriostatic water should I add to a peptide vial?
Most people add 2mL to 3mL of bacteriostatic water per vial, but the right amount depends on the dose you want to draw and the syringe size you are using. Adding 1mL to a 5mg vial gives you a concentration of 5,000 mcg/mL, making each dose very small in volume. Adding 2mL gives you 2,500 mcg/mL, which is easier to measure on a standard insulin syringe. A general guideline is to choose a volume that puts your typical dose somewhere between 10 and 30 units on a U-100 syringe. Use the calculator above to test different water volumes and find what works for your dose.
How are peptides different from proteins?
Both are made of amino acids, but peptides are much smaller than proteins. Because of their tiny size, peptides can act like tiny messengers in the body, sending specific signals to your cells to tell them exactly what to do.
GlutathioneResearch References
Glutathione is a phase 2 compound
Glutathione
Glutathione is a phase 2 compound
IV Glutathione in Parkinson's Disease
IV glutathione 1,400 mg 3x weekly showed possible mild symptomatic effect in small trial.
n.d.
Liposomal Glutathione in HIV Patients
13-week supplementation improved Th1 cytokines including IL-12, IFN-γ, and TNF-α.
n.d.
Glutathione Supplementation for Skin Lightening
Narrative review of efficacy and safety for skin lightening applications.
n.d.
Glutathione Metabolism and Function
Comprehensive review of glutathione as master antioxidant and its roles in health.
n.d.
Calculate peptide dosages
Learning how to calculate a peptide dose? Use our beginner-friendly peptide dosage, blend, and accumulation calculators. Enter vial size, reconstitution volume, and target dose to get exact draw volumes instantly. No guesswork, just clear guidance that helps prevent common mistakes.
