Longevity
Pancragen
Metabolic
4
Amino acids
Molecular weight
Peptide
Type
Pancragen is a synthetic tetrapeptide bioregulator (Lys-Glu-Asp-Trp; KEDW) derived from bovine pancreatic tissue, developed by Professor Vladimir Khavinson at the St. Petersburg Institute of Bioregulation and Gerontology. It modulates pancreatic gene expression through epigenetic mechanisms, upregulating transcription factors critical for beta-cell differentiation and function. It is used in research contexts targeting pancreatic health, blood sugar regulation, and age-related metabolic decline.
Top researched benefits
Overview of Pancragen
Pancragen acts as an epigenetic regulator by interacting with chromatin complexes and DNA structures to activate transcription factors — including Pdx1, the master regulator of pancreatic development — thereby promoting beta-cell differentiation, renewal, and insulin-secretory capacity rather than acting directly on insulin receptors or sulfonylurea pathways.
metabolic support
- Corrects age-related glucose tolerance disturbances in primate studies.
- Reduces incidence and magnitude of metabolic syndrome.
- Helps control blood sugar through pancreatic function support.
pancreatic health
- Impacts differentiation of pancreatic cells during aging.
- Improves endocrine function of the pancreas.
- Addresses age-related imbalance of pancreatic function.
antiaging
- Directly interacts with DNA to regulate pancreatic gene expression.
Typical Dose
500mcg per dose
Frequency
twice daily
Cycle Duration
28-day cycle, repeated every 3–6 months
Storage
Lyophilized: 2–8°C. Reconstituted with bacteriostatic water: 2–8°C, use within 14–28 days. Discard if solution becomes cloudy or discolored.
Chemical Makeup
Key benefits
Upregulates Pdx1 and other pancreatic transcription factors to promote beta-cell differentiation and renewal in aging or damaged pancreatic tissue
Improves glucose clearance and normalizes insulin and C-peptide responses to glucose challenge, as demonstrated in rhesus monkey studies
Reduces fasting blood glucose and improves markers of insulin resistance in elderly type 2 diabetes patients in limited human study data
Protects mesenteric capillary endothelium in streptozotocin-induced diabetic rat models, suggesting endothelioprotective effects alongside glucose-lowering action
Community interest
This peptide is still gaining traction in the community.
Pancreatic Bioregulator | Metabolic & Blood Sugar Support
This overview is informational and based on aggregated descriptions from studies and user reports.
Was it helpful?YesNoPancragen Molecular Information
View the scientifc details of Pancragen.
4
Amino Acids
Pancragen
Lys
Lys
Position 1
Glu
Glu
Position 2
Asp
Asp
Position 3
Trp
Trp
Position 4
Molecular Weight
576.25DaChain Length
4Amino AcidsType
PeptidePancragen Protocols
Subcutaneous or intramuscular injection is the primary research delivery route for Pancragen, ensuring the KEDW tetrapeptide reaches systemic circulation intact to bind DNA promoter regions and activate pancreatic islet cell gene transcription. Cycles are short — 10 to 20 days — with 2–3 month rest periods between courses, following the Khavinson bioregulator protocol.
| Goal | Dosage | Frequency | Route |
|---|---|---|---|
| Restore pancreatic endocrine function and normalize glucose tolerance in early metabolic dysfunction | 500 | 1 day range | IM |
| Support pancreatic beta-cell function and improve insulin sensitivity in impaired glucose tolerance | 1000 | 1 day range | SubQ |
| Reduce insulin resistance and normalize glucose-stimulated insulin secretion in metabolic syndrome | 1000 | 2 day range | SubQ |
| Support pancreatic tissue regeneration and reduce inflammation in chronic pancreatitis | 1000 | 1 day range | SubQ |
| Slow age-related pancreatic endocrine decline and preserve insulin secretion capacity | 1000 | 1 day range | SubQ |
Reconstitution Instructions
Materials needed:
Steps to reconstitute
- Allow the Pancragen vial and bacteriostatic water to reach room temperature before reconstitution
- Gently tap the vial to settle the lyophilized powder at the bottom
- Wipe the rubber stopper of the vial with an alcohol swab and allow it to dry
- Draw up 2 mL of bacteriostatic water into a reconstitution syringe (for a 20 mg vial this yields a 10 mg/mL concentration)
- Insert the needle into the vial at an angle and inject the bacteriostatic water slowly down the inside wall — never directly onto the powder
- Gently swirl or roll the vial for 15–30 seconds until the powder is fully dissolved — do not shake
- Inspect the solution for complete dissolution; the reconstituted solution should be clear and colorless
- Draw the desired dose into an insulin syringe using sterile technique
- For SubQ injection, choose the abdomen (at least 2 inches from the navel) or outer thigh; insert needle at 45 degrees and inject slowly
- For IM injection, use the upper outer thigh or deltoid; insert needle at 90 degrees and inject slowly
- Clean the injection site with an alcohol swab before each injection and rotate sites to prevent tissue irritation
- Store reconstituted vial refrigerated at 36–46°F (2–8°C) and use within 14–28 days
Pancragen Cycle
The Pancragen Cycle section explains how long a typical cycle lasts and what to expect during each phase. Over time, your body can become less responsive with continuous use.
Taking breaks between cycles may help maintain effectiveness and support better overall results.
- Week 0-0
- Gene expression modulation begins
- Week 0-0
- Effects persist due to epigenetic changes
- Week 0-0
- Glucose tolerance and pancreatic function improvements
- Week 0-0
- Cumulative benefits with periodic cycles
Dosing tools
Pancragen Peptide Dosage Calculator
Calculate peptide doses with our visual syringe guide.
mg
Enter the total amount of peptide in the vial in milligrams (as stated on the label).
The dose you want to inject per administration, in mcg or mg.
1,000 mcg = 1 mg
1 mL
2 mL
3 mL
5 mL
Custom
Volume of bacteriostatic water you add to reconstitute the powder. Use BAC water for preservation.
Injection Results
Based on your vial and dilution inputs.
Pancragen
SINGLE COMPOUNDVolume per injection
0.05
mL
Concentration
10.00
mg/mL
Doses per vial
20
doses
Total injections per vial
20 injections
How it works
Based on a 10 mg Pancragen vial diluted with 1 mL of bacteriostatic water, each 500 mcg injection equals 0.05 mL.
1mL / 100 units
5 units
0.050 mL
Reference Guide
Dosing Cycle
- Peptide
- Pancragen
- Dosing
- 500mcg per dose
- Dosing Frequency
- twice daily
- Cycle Duration
- 28-day cycle, repeated every 3–6 months
- Storage
- Lyophilized: 2–8°C. Reconstituted with bacteriostatic water: 2–8°C, use within 14–28 days. Discard if solution becomes cloudy or discolored.
Note: Triple agonist; microdose for fewer side effects
Reconstitution Tips
- Use bacteriostatic water (BAC) — contains 0.9% benzyl alcohol for preservation
- Inject water slowly — aim down the vial wall, not directly onto powder
- Never shake — gently swirl or roll the vial until dissolved
- Store properly — refrigerate at 2-8°C after reconstitution
- Use within 28 days — most reconstituted peptides remain stable for about 4 weeks
- Keep sterile — always clean vial tops with alcohol before drawing
Peptide Interactions
Research suggestions of Pancragen interactions with other common peptides and substances.
Longevity
epi
Immunity
Epitalon
SYNERGISTIC
Often combined in comprehensive anti-aging Khavinson protocols.
Longevity
liv
Immunity
Livagen
COMPATIBLE
Different organ targets; can be used in comprehensive bioregulator protocols.
Longevity
vil
Immunity
Vilon
COMPATIBLE
Part of Khavinson bioregulator family; targets different tissue.
Side effects
Contraindications
Active pancreatic emergencies (seek medical care)
Known hypersensitivity
Pregnancy or breastfeeding
Type 1 diabetes (consult physician)
Stop signs
Allergic reactions
Unusual blood sugar changes
Signs of hypoglycemia
Bad signs
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Frequently asked questions
What is a peptide dosage calculator?
A peptide dosage calculator is a free tool that converts your vial size, bacteriostatic water volume, and target dose into an exact syringe draw volume. Instead of doing the reconstitution math by hand, you enter three inputs and instantly get the concentration of your solution and how many milliliters or syringeunits to draw. This calculator works for single peptide compounds and multi-peptide blends.
How do I calculate peptide dosage from a vial?
To calculate your peptide dose, divide the total peptide content of your vial in micrograms by the volume of bacteriostatic water you added in milliliters. This gives you your solution concentration in mcg/mL. Then divide your target dose by that concentration to get your draw volume. For example, a 5mg (5,000 mcg) vial reconstituted with 2mL of BAC water gives a concentration of 2,500 mcg/mL. A 250 mcg dose would require drawing 0.1mL. This calculator automates all of those steps instantly.
How much Bacteriostatic water should I add to a peptide vial?
Most people add 2mL to 3mL of bacteriostatic water per vial, but the right amount depends on the dose you want to draw and the syringe size you are using. Adding 1mL to a 5mg vial gives you a concentration of 5,000 mcg/mL, making each dose very small in volume. Adding 2mL gives you 2,500 mcg/mL, which is easier to measure on a standard insulin syringe. A general guideline is to choose a volume that puts your typical dose somewhere between 10 and 30 units on a U-100 syringe. Use the calculator above to test different water volumes and find what works for your dose.
How are peptides different from proteins?
Both are made of amino acids, but peptides are much smaller than proteins. Because of their tiny size, peptides can act like tiny messengers in the body, sending specific signals to your cells to tell them exactly what to do.
PancragenResearch References
Pancragen is a preclinical compound
Pancragen
Pancragen is a preclinical compound
Correction of Impaired Glucose Tolerance Using Tetrapeptide (Pancragen) in Old Female Rhesus Monkeys
Pancragen normalized insulin and C-peptide levels, suggesting recovery of disturbed glucose tolerance in old animals.
n.d.
Tetrapeptide KEDW Interacts with DNA and Regulates Gene Expression
KEDW directly interacts with DNA and regulates pancreatic gene expression.
n.d.
Impacts of Pancragen on Differentiation of Pancreatic Cells During Aging
Pancragen affects pancreatic cell differentiation during aging process.
n.d.
Prospects of Using Pancragen for Metabolic Disorders in Elderly
Pancragen shows promise for correction of metabolic disorders in elderly people.
n.d.
Calculate peptide dosages
Learning how to calculate a peptide dose? Use our beginner-friendly peptide dosage, blend, and accumulation calculators. Enter vial size, reconstitution volume, and target dose to get exact draw volumes instantly. No guesswork, just clear guidance that helps prevent common mistakes.
