Longevity
Testagen
Sexual
4
Amino acids
Molecular weight
Peptide
Type
Testagen (KEDG) is a synthetic tetrapeptide bioregulator composed of lysine, glutamic acid, aspartic acid, and glycine, originally isolated from testicular tissue extracts by the Khavinson research group. It works epigenetically by penetrating cell and nuclear membranes to interact directly with DNA, modulating transcription in the hypothalamic-pituitary-gonadal (HPG) axis and thyroid tissues. It is used in research and biohacking contexts primarily by men seeking to support endogenous testosterone and thyroid hormone regulation without exogenous hormone replacement.
Top researched benefits
Overview of Testagen
Testagen crosses cellular and nuclear membranes and binds directly to DNA regulatory regions — particularly CAAC-rich sequences — modulating transcription of genes governing pituitary-gonadal signaling, Leydig cell steroidogenesis, and TSH-mediated thyroid hormone synthesis, without acting through classical cell-surface receptor pathways.
male hormone support
- Promotes testosterone biosynthesis through Leydig cell stimulation.
- Restores normal communication within hypothalamic-pituitary-gonadal axis.
- Addresses declining testosterone through cellular metabolism improvement.
male reproductive health
- Supports spermatogenesis and reproductive balance.
- Normalizes testicular function by reducing peptide deficiency.
- May enhance male sexual function through testosterone support.
prostate support
- Research shows reduction in inflammation markers in prostate tissue.
Typical Dose
100–300mcg per injection
Frequency
once daily
Cycle Duration
8–12 weeks on, equivalent break between cycles
Storage
Lyophilized: −20°C in dry, dark conditions. Reconstituted: 2–8°C, avoid freeze-thaw cycles; use within 3–4 weeks.
Chemical Makeup
Key benefits
Stimulates endogenous testosterone production by modulating gene expression in Leydig cells of the testes without suppressing the HPG axis
Supports TSH release and increases T3/T4 thyroid hormone levels, demonstrated in hypophysectomized animal models with impaired pituitary signaling
In a preliminary clinical report (Rossikhin et al., 2011), improved uroflowmetry indicators and reduced prostatic inflammation in men with chronic abacterial prostatitis alongside elevation in serum testosterone
Observed to increase telomerase activity in reproductive and thyroid tissues in aged animal studies within the Khavinson bioregulator program; not yet confirmed for KEDG specifically in controlled human trials
Community interest
This peptide is still gaining traction in the community.
Epigenetic Bioregulator | HPG Axis & Thyroid Modulation
This overview is informational and based on aggregated descriptions from studies and user reports.
Was it helpful?YesNoTestagen Molecular Information
View the scientifc details of Testagen.
4
Amino Acids
Testagen
Lys
Lys
Position 1
Glu
Glu
Position 2
Asp
Asp
Position 3
Gly
Gly
Position 4
Molecular Weight
447.44DaChain Length
4Amino AcidsType
PeptideTestagen Protocols
Subcutaneous injection is the primary and most reliable delivery route for Testagen, a tetrapeptide (Lys-Glu-Asp-Gly) that works at the epigenetic level to stimulate Leydig cell steroidogenesis and restore HPG axis signaling. Morning administration into the abdomen, thigh, or upper arm is standard, with sites rotated to prevent tissue irritation over 10–20 day cycles.
| Goal | Dosage | Frequency | Route |
|---|---|---|---|
| Conservative dose to assess individual tolerance before escalating; establishes baseline HPG axis response | 100 | 1 day range | SubQ |
| Stimulate Leydig cell steroidogenesis and normalize testosterone levels in men with sub-optimal endocrine function | 200 | 1 day range | SubQ |
| Address significant testicular function decline with a higher daily dose for a full 10–20 day course | 300 | 1 day range | SubQ |
| Restore HPG axis signaling and endogenous testosterone production following suppressive anabolic cycles | 200 | 1 day range | SubQ |
| Improve spermatogenesis and reproductive hormone markers by upregulating steroidogenic enzyme expression in Sertoli and Leydig cells | 250 | 1 day range | SubQ |
| Sustain testosterone levels and HPG axis responsiveness between full courses using lower-dose repeat cycles every 3–6 months | 100 | 1 day range | SubQ |
Reconstitution Instructions
Materials needed:
Steps to reconstitute
- Allow the lyophilized Testagen vial to reach room temperature before opening to reduce condensation inside the vial
- Draw 3.0 mL of bacteriostatic water into a sterile 3–5 mL syringe fitted with a 23–25 gauge needle
- Insert the needle through the vial's rubber stopper and inject the bacteriostatic water slowly down the inside wall of the vial — never directly onto the powder
- Remove the syringe and gently swirl or roll the vial until the powder is fully dissolved — do not shake, as shaking degrades the peptide
- The resulting concentration is approximately 6.67 mg/mL (6670 mcg/mL); on a U-100 insulin syringe, 1 unit equals approximately 66.7 mcg
- Use a 30- or 50-unit insulin syringe for dosing to achieve accurate measurements at low mcg volumes (e.g., 100 mcg = 1.5 units)
- Store the reconstituted vial refrigerated at 2–8°C, protected from light, and use within 3–4 weeks; discard if cloudy or particulate matter is visible
- Clean the injection site with an alcohol swab and allow to dry fully before injecting
- Pinch a skinfold and insert the insulin syringe needle at a 45–90° angle into subcutaneous tissue
- Inject slowly and steadily, then withdraw the needle and apply light pressure with a swab — do not aspirate for subcutaneous injections
- Rotate injection sites systematically among the abdomen (avoiding 2 inches around the navel), thighs, and upper arms each day
Testagen Cycle
The Testagen Cycle section explains how long a typical cycle lasts and what to expect during each phase. Over time, your body can become less responsive with continuous use.
Taking breaks between cycles may help maintain effectiveness and support better overall results.
- Week 0-0
- Gene expression modulation and cellular metabolism changes begin
- Week 0-0
- Effects persist due to epigenetic changes
- Week 0-0
- Testosterone and reproductive function improvements
- Week 0-0
- Cumulative benefits with periodic cycles
Dosing tools
Testagen Peptide Dosage Calculator
Calculate peptide doses with our visual syringe guide.
mg
Enter the total amount of peptide in the vial in milligrams (as stated on the label).
The dose you want to inject per administration, in mcg or mg.
1,000 mcg = 1 mg
1 mL
2 mL
3 mL
5 mL
Custom
Volume of bacteriostatic water you add to reconstitute the powder. Use BAC water for preservation.
Injection Results
Based on your vial and dilution inputs.
Testagen
SINGLE COMPOUNDVolume per injection
0.05
mL
Concentration
10.00
mg/mL
Doses per vial
20
doses
Total injections per vial
20 injections
How it works
Based on a 10 mg Testagen vial diluted with 1 mL of bacteriostatic water, each 500 mcg injection equals 0.05 mL.
1mL / 100 units
5 units
0.050 mL
Reference Guide
Dosing Cycle
- Peptide
- Testagen
- Dosing
- 100–300mcg per injection
- Dosing Frequency
- once daily
- Cycle Duration
- 8–12 weeks on, equivalent break between cycles
- Storage
- Lyophilized: −20°C in dry, dark conditions. Reconstituted: 2–8°C, avoid freeze-thaw cycles; use within 3–4 weeks.
Note: Triple agonist; microdose for fewer side effects
Reconstitution Tips
- Use bacteriostatic water (BAC) — contains 0.9% benzyl alcohol for preservation
- Inject water slowly — aim down the vial wall, not directly onto powder
- Never shake — gently swirl or roll the vial until dissolved
- Store properly — refrigerate at 2-8°C after reconstitution
- Use within 28 days — most reconstituted peptides remain stable for about 4 weeks
- Keep sterile — always clean vial tops with alcohol before drawing
Peptide Interactions
Research suggestions of Testagen interactions with other common peptides and substances.
Longevity
epi
Immunity
Epitalon
SYNERGISTIC
Often combined in comprehensive anti-aging Khavinson protocols.
Sexual
gon
Hormonal
Gonadorelin
SYNERGISTIC
Both support HPG axis function through different mechanisms.
Sexual
pro
Prostamax
SYNERGISTIC
Complementary male reproductive bioregulators; different tissue targets.
Side effects
Contraindications
Hormone-sensitive cancers (consult oncologist)
Known hypersensitivity
Female use not established
Stop signs
Allergic reactions
Signs of hormone imbalance
Bad signs
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Frequently asked questions
What is a peptide dosage calculator?
A peptide dosage calculator is a free tool that converts your vial size, bacteriostatic water volume, and target dose into an exact syringe draw volume. Instead of doing the reconstitution math by hand, you enter three inputs and instantly get the concentration of your solution and how many milliliters or syringeunits to draw. This calculator works for single peptide compounds and multi-peptide blends.
How do I calculate peptide dosage from a vial?
To calculate your peptide dose, divide the total peptide content of your vial in micrograms by the volume of bacteriostatic water you added in milliliters. This gives you your solution concentration in mcg/mL. Then divide your target dose by that concentration to get your draw volume. For example, a 5mg (5,000 mcg) vial reconstituted with 2mL of BAC water gives a concentration of 2,500 mcg/mL. A 250 mcg dose would require drawing 0.1mL. This calculator automates all of those steps instantly.
How much Bacteriostatic water should I add to a peptide vial?
Most people add 2mL to 3mL of bacteriostatic water per vial, but the right amount depends on the dose you want to draw and the syringe size you are using. Adding 1mL to a 5mg vial gives you a concentration of 5,000 mcg/mL, making each dose very small in volume. Adding 2mL gives you 2,500 mcg/mL, which is easier to measure on a standard insulin syringe. A general guideline is to choose a volume that puts your typical dose somewhere between 10 and 30 units on a U-100 syringe. Use the calculator above to test different water volumes and find what works for your dose.
How are peptides different from proteins?
Both are made of amino acids, but peptides are much smaller than proteins. Because of their tiny size, peptides can act like tiny messengers in the body, sending specific signals to your cells to tell them exactly what to do.
TestagenResearch References
Testagen is an experimental compound
Testagen
Testagen is an experimental compound
Testagen Bioregulator Research on Testosterone and Leydig Cells
Testagen promotes testosterone biosynthesis by stimulating Leydig cell activity and improving testicular metabolism.
n.d.
Short Peptides and HPG Axis Function
KEDG peptide helps restore communication within hypothalamic-pituitary-gonadal axis.
n.d.
Epigenetic Effects of Short Peptides
Testagen penetrates cell nuclei and interacts with DNA, recognizing methylation status.
n.d.
Khavinson Peptide Bioregulators
Comprehensive review of bioregulator peptides isolated from testicular and other tissue extracts.
n.d.
Calculate peptide dosages
Learning how to calculate a peptide dose? Use our beginner-friendly peptide dosage, blend, and accumulation calculators. Enter vial size, reconstitution volume, and target dose to get exact draw volumes instantly. No guesswork, just clear guidance that helps prevent common mistakes.
