Longevity
Testagen
Sexual
4
Amino acids
Molecular weight
Peptide
Type
Testagen is a Khavinson bioregulator tetrapeptide (KEDG) originally isolated from testicular tissue extracts. Developed by Professor Vladimir Khavinson at the St. Petersburg Institute of Bioregulation and Gerontology, it promotes testosterone biosynthesis by stimulating Leydig cell activity and improving cellular metabolism in testicular tissue. Research shows it helps restore normal communication within the hypothalamic-pituitary-gonadal (HPG) axis, governing testosterone production and spermatogenesis.
Top researched benefits
Overview of Testagen
Testagen works through epigenetic regulation, crossing both cell and nuclear membranes to interact directly with DNA of target tissues. It penetrates into cytoplasm, nucleus, and nucleolus of cells, interacting with various cellular components including DNA and RNA. The peptide can discriminate between different nucleotide sequences and recognize cytosine methylation status. It interacts with the anterior pituitary gland and endocrine pathways involved in testosterone and TSH synthesis, restoring HPG axis communication.
male hormone support
- Promotes testosterone biosynthesis through Leydig cell stimulation.
- Restores normal communication within hypothalamic-pituitary-gonadal axis.
- Addresses declining testosterone through cellular metabolism improvement.
male reproductive health
- Supports spermatogenesis and reproductive balance.
- Normalizes testicular function by reducing peptide deficiency.
- May enhance male sexual function through testosterone support.
prostate support
- Research shows reduction in inflammation markers in prostate tissue.
Typical Dose
10-20mg daily during cycle
Frequency
Daily for 10-20 days, then break
Cycle Duration
10-20 day cycles (typical bioregulator protocol)
Storage
Capsules: room temperature. Reconstituted: 2-8°C
Chemical Makeup
Key benefits
Promotes testosterone biosynthesis
Stimulates Leydig cell activity
Improves testicular cellular metabolism
Restores HPG axis communication
Supports spermatogenesis
Reduces prostatic inflammation
Enhances male reproductive balance
May normalize testosterone at any age
Community interest
This peptide is still gaining traction in the community.
KEDG Tetrapeptide | Testicular Bioregulator
This overview is informational and based on aggregated descriptions from studies and user reports.
Was it helpful?YesNoTestagen Molecular Information
View the scientifc details of Testagen.
4
Amino Acids
Testagen
Lys
Lys
Position 1
Glu
Glu
Position 2
Asp
Asp
Position 3
Gly
Gly
Position 4
Molecular Weight
419DaChain Length
4Amino AcidsType
PeptideTestagen Protocols
Available in capsule form for oral administration. Short peptides can be absorbed orally and reach target tissues. Typical protocol involves 10-20 day cycles.
| Goal | Dosage | Frequency | Route |
|---|---|---|---|
| Standard protocol | 10 | 10 week range | Oral capsules |
| Maintenance | 10 | 2 week range | Oral capsules |
Testagen Cycle
The Testagen Cycle section explains how long a typical cycle lasts and what to expect during each phase. Over time, your body can become less responsive with continuous use.
Taking breaks between cycles may help maintain effectiveness and support better overall results.
- Week 0-0
- Gene expression modulation and cellular metabolism changes begin
- Week 0-0
- Effects persist due to epigenetic changes
- Week 0-0
- Testosterone and reproductive function improvements
- Week 0-0
- Cumulative benefits with periodic cycles
Dosing tools
Testagen Peptide Dosage Calculator
Calculate peptide doses with our visual syringe guide.
mg
Enter the total amount of peptide in the vial in milligrams (as stated on the label).
The dose you want to inject per administration, in mcg or mg.
1,000 mcg = 1 mg
1 mL
2 mL
3 mL
5 mL
Custom
Volume of bacteriostatic water you add to reconstitute the powder. Use BAC water for preservation.
Injection Results
Based on your vial and dilution inputs.
Testagen
SINGLE COMPOUNDVolume per injection
0.05
mL
Concentration
10.00
mg/mL
Doses per vial
20
doses
Total injections per vial
20 injections
How it works
Based on a 10 mg Testagen vial diluted with 1 mL of bacteriostatic water, each 500 mcg injection equals 0.05 mL.
1mL / 100 units
5 units
0.050 mL
Reference Guide
Dosing Cycle
- Peptide
- Testagen
- Dosing
- 10-20mg daily during cycle
- Dosing Frequency
- Daily for 10-20 days, then break
- Cycle Duration
- 10-20 day cycles (typical bioregulator protocol)
- Storage
- Capsules: room temperature. Reconstituted: 2-8°C
Note: Triple agonist; microdose for fewer side effects
Reconstitution Tips
- Use bacteriostatic water (BAC) — contains 0.9% benzyl alcohol for preservation
- Inject water slowly — aim down the vial wall, not directly onto powder
- Never shake — gently swirl or roll the vial until dissolved
- Store properly — refrigerate at 2-8°C after reconstitution
- Use within 28 days — most reconstituted peptides remain stable for about 4 weeks
- Keep sterile — always clean vial tops with alcohol before drawing
Peptide Interactions
Research suggestions of Testagen interactions with other common peptides and substances.
Longevity
epi
Immunity
Epitalon
SYNERGISTIC
Often combined in comprehensive anti-aging Khavinson protocols.
Sexual
gon
Hormonal
Gonadorelin
SYNERGISTIC
Both support HPG axis function through different mechanisms.
Sexual
pro
Prostamax
SYNERGISTIC
Complementary male reproductive bioregulators; different tissue targets.
Side effects
Contraindications
Hormone-sensitive cancers (consult oncologist)
Known hypersensitivity
Female use not established
Stop signs
Allergic reactions
Signs of hormone imbalance
Bad signs
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Frequently asked questions
What is a peptide dosage calculator?
A peptide dosage calculator is a free tool that converts your vial size, bacteriostatic water volume, and target dose into an exact syringe draw volume. Instead of doing the reconstitution math by hand, you enter three inputs and instantly get the concentration of your solution and how many milliliters or syringeunits to draw. This calculator works for single peptide compounds and multi-peptide blends.
How do I calculate peptide dosage from a vial?
To calculate your peptide dose, divide the total peptide content of your vial in micrograms by the volume of bacteriostatic water you added in milliliters. This gives you your solution concentration in mcg/mL. Then divide your target dose by that concentration to get your draw volume. For example, a 5mg (5,000 mcg) vial reconstituted with 2mL of BAC water gives a concentration of 2,500 mcg/mL. A 250 mcg dose would require drawing 0.1mL. This calculator automates all of those steps instantly.
How much Bacteriostatic water should I add to a peptide vial?
Most people add 2mL to 3mL of bacteriostatic water per vial, but the right amount depends on the dose you want to draw and the syringe size you are using. Adding 1mL to a 5mg vial gives you a concentration of 5,000 mcg/mL, making each dose very small in volume. Adding 2mL gives you 2,500 mcg/mL, which is easier to measure on a standard insulin syringe. A general guideline is to choose a volume that puts your typical dose somewhere between 10 and 30 units on a U-100 syringe. Use the calculator above to test different water volumes and find what works for your dose.
How are peptides different from proteins?
Both are made of amino acids, but peptides are much smaller than proteins. Because of their tiny size, peptides can act like tiny messengers in the body, sending specific signals to your cells to tell them exactly what to do.
TestagenResearch References
Testagen is a moderate research compound
Testagen
Testagen is a moderate research compound
Testagen Bioregulator Research on Testosterone and Leydig Cells
Testagen promotes testosterone biosynthesis by stimulating Leydig cell activity and improving testicular metabolism.
n.d.
Short Peptides and HPG Axis Function
KEDG peptide helps restore communication within hypothalamic-pituitary-gonadal axis.
n.d.
Epigenetic Effects of Short Peptides
Testagen penetrates cell nuclei and interacts with DNA, recognizing methylation status.
n.d.
Khavinson Peptide Bioregulators
Comprehensive review of bioregulator peptides isolated from testicular and other tissue extracts.
n.d.