Reviewed by the PeptideMind Team · Updated July 15, 2026

Humanin Dosage Guide, Benefits & Side Effects

Humanin is studied for protecting neurons and cardiac cells from stress-induced apoptosis, with higher levels found in centenarians.

Chemical Makeup

24

Amino Acids

Humanin

Met

M

Position 1

Ala

A

Position 2

Pro

P

Position 3

Arg

R

Position 4

Gly

G

Position 5

Phe

F

Position 6

Ser

S

Position 7

Cys

C

Position 8

Leu

L

Position 9

Leu

L

Position 10

Leu

L

Position 11

Leu

L

Position 12

Thr

T

Position 13

Ser

S

Position 14

Glu

E

Position 15

Ile

I

Position 16

Asp

D

Position 17

Leu

L

Position 18

Pro

P

Position 19

Val

V

Position 20

Lys

K

Position 21

Arg

R

Position 22

Arg

R

Position 23

Ala

A

Position 24

Amino acid sequence
MethioninePosition 1
AlaninePosition 2
ProlinePosition 3
ArgininePosition 4
GlycinePosition 5
PhenylalaninePosition 6
SerinePosition 7
CysteinePosition 8
LeucinePosition 9
LeucinePosition 10
LeucinePosition 11
LeucinePosition 12
ThreoninePosition 13
SerinePosition 14
Glutamic acidPosition 15
IsoleucinePosition 16
Aspartic acidPosition 17
LeucinePosition 18
ProlinePosition 19
ValinePosition 20
LysinePosition 21
ArgininePosition 22
ArgininePosition 23
AlaninePosition 24

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Humanin Dosage Guide for Longevity & Anti-Aging

Humanin helps protect cells, particularly by supporting healthy mitochondria.

Vial size

10 mg

Bacteriostatic water

3 mL

Dosing

10 mg

Frequency

1–2×/week

Cycle

4–6 weeks

Benefit

Longevity & Anti-Aging

Explore calculators

What is Humanin?

Humanin is a small peptide made inside your mitochondria (the energy factories of your cells) and is strongly linked to longevity: people who live to 100 tend to have higher levels of it. It appears to protect cells from dying and helps shield nerve cells and metabolic function from stress and damage. Researchers and longevity-focused clinicians are interested in it for protecting the brain, supporting metabolism, and potentially slowing cellular aging.

Cognitive

Research targets include neural signaling, memory formation, and mental clarity pathways.

Longevity

Research targets include cellular aging, senescence, and long-term healthspan.

Longevity

hum

Longevity

Is Humanin FDA approved?

Humanin is not an FDA-approved drug. It is intended for research purposes only and is not approved for human consumption, diagnostic, or therapeutic use.

Research Use Only

Humanin has not been evaluated by the FDA for safety or efficacy in humans.

No Clinical Oversight

Not manufactured under FDA-regulated quality or clinical standards.

Unregulated Sourcing

Purity, dosing, and sourcing are not verified through FDA testing or oversight.

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How Does Humanin Work?

It attaches to a receptor complex on cells that switches on several survival pathways, helping protect cells from dying off and from damage caused by unstable molecules. Inside the cell, it also travels into the mitochondria (the energy factories) to keep them working properly and prevent early cell aging.

Binds a survival receptor

Attaches to a receptor complex that switches on cell-survival pathways.

Protects from damage

Helps protect cells from dying off and from unstable molecules.

Supports mitochondria

Travels into mitochondria to keep them running and prevent early cell aging.

Humanin Targeted areas

How to reconstitute Humanin

View guide

The materials you'll need and step-by-step instructions for safely mixing Humanin with bacteriostatic water.

Materials needed

Your Humanin vial (lyophilized)

Alcohol swabs

Bacteriostatic sterile water

3 mL syringes (Luer Lock tip)

25G or 27G needles (Luer Lock). Other gauges may also be acceptable.

Sharps container (optional)

1

Remove the caps

Remove the caps from both your Humanin vial and your bacteriostatic water vial.
2

Sanitize the rubber stoppers

Wipe down the rubber stoppers on both vials with alcohol swabs and let them dry for about 3 minutes.
3

Attach the needle

Take your 3 mL syringe and needle. Twist the needle's plastic hub into the Luer Lock tip on the syringe to secure it.
4

Draw the bac water

Pull back on the plunger to draw your desired volume of bacteriostatic water. If you overfill, just push the excess back in until you reach the right marker on the syringe.

How much bac water? Humanin typically comes in a 10 mg vial. We recommend 3 mL of bacteriostatic water, though anywhere between 1 mL and 3 mL is acceptable. For larger vials you can use up to 5 mL — more bac water makes microdosing easier, but insulin needles usually max out at 1 mL per injection. That means a lot of bac water might force you into multiple injections to hit your target dose. Unless you're microdosing, 2 mL per vial is a solid rule of thumb.
5

Insert the needle into the Humanin vial

With the bac water in your syringe, insert the needle into the Humanin vial at a slight angle to avoid pressure buildup.

Use a thin needle: Stick to 25G or higher to avoid damaging the rubber stopper. Higher gauge means a thinner needle.
6

Release the water gently

Let the water run gently down the side of the vial. Don't inject it forcefully.

Heads up: Sometimes the plunger pushes itself in as soon as the needle enters the vial. Hold the plunger back so you have full control over how fast the bac water enters.
7

Swirl to dissolve

Avoid shaking. Gently swirl, flip, and roll the vial to dissolve the powder.

Be patient: This usually takes 2 to 5 minutes of consistent swirling, mixing in a circular motion, and gently flipping the vial up and down. The water needs to run through the powder many times to fully dissolve it. Don't shake or get aggressive with it.
8

Check for full dissolution

The solution should be clear with no visible particles. If it's cloudy or clumpy, wait a minute and repeat the swirling step.
9

Cap, dispose, and store

Once you're done, cap the needle and dispose of it (ideally in a sharps container). Store the reconstituted Humanin vial in the fridge whenever it's not in use. It will stay good for approximately 30 days.

How to Store Humanin

Freeze the dry powder for long-term storage, or keep it in the fridge for shorter periods. Once mixed with liquid, refrigerate and use within 30–60 days, protected from light and repeated freezing and thawing.

Lyophilized Storage

-20°C long-term, or 4°C short-term.

Reconstituted Storage

2–8°C for up to 30–60 days.

Handling Notes

Protect from light and repeated freeze-thaw cycles.

A smartphone displaying a "Storage Guide" for peptides, indicating a safe storage temperature range of 2–8°C, with warnings to keep away from light, heat, and freezing. The background is light blue, and additional tips on protecting the peptides are visible below the temperature range.

What Are the Benefits of Humanin?

What research says it may help with, and how it works in the body.

neuroprotection

Encourages cells to produce more of their energy-generating mitochondria, working through an internal cell-signaling pathway, in Parkinson's disease models.

Offers wide-ranging protection to nerve cells against many different kinds of damaging insults.

First discovered because it protects brain cells from damage caused by amyloid-beta, the sticky protein buildup linked to Alzheimer's disease.

longevity

Producing extra amounts of it extends lifespan in roundworms, an effect that depends on a specific longevity-related genetic pathway.

People who live to 100 and their children tend to have higher levels of it, suggesting a link to long life.

A lab-modified version of it improved metabolic health measures in middle-aged mice.

cellular protection

Protects many different kinds of cells from damage and death caused by stress.

Blocks proteins that trigger programmed cell death, helping prevent cells from self-destructing.

Supports the health of mitochondria, the cell's energy-producing structures, and encourages the cell to make more of them.

What Are the Side Effects of Humanin?

Who should avoid it, warning signs to watch for, and what to know before combining it with other compounds.

Who Should Avoid It

Active cancer (theoretical concern with anti-apoptotic effects)

Pregnancy or breastfeeding

Hypersensitivity to peptide components

Stop Right Away If You Notice

Signs of allergic reaction

Unusual persistent symptoms

Milder Signs to Watch For

Discolored powderCloudy or particulate solutionBroken seal
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How Long Should a Humanin Cycle Last?

This breaks down how long a typical Humanin cycle runs and what research suggests happens at each stage. Research shows that staying on a peptide continuously, without a break, may make it less effective over time.

That's why most research protocols build in a break between cycles, often called a washout period, to let the body reset before starting again.

Week 1-2
Subtle; peptide begins working at cellular level
Week 2-4
Improved energy and reduced fatigue may begin
Week 4-8
Enhanced cellular resilience and stress response
Week 8-12
Cumulative benefits in mitochondrial function and healthspan markers
Week 0-0
Potential longevity and neuroprotective benefits

Cheat Sheets Featuring Humanin

See how Humanin fits into a broader research stack with these free downloadable cheat sheets. Each one includes reference dose ranges, dosing frequency, and half-life for every peptide it covers. For research purposes only.

View all
Cognitive Peptide Cheat Sheet
Cognitive Peptide Cheat Sheet

16 peptides

Longevity Peptide Cheat Sheet
Longevity Peptide Cheat Sheet

42 peptides

Master Peptide Cheat Sheet
Master Peptide Cheat Sheet

78 peptides

Humanin Research References

It is a preclinical compound

4Research references

Humanin

Humanin is a preclinical compound

Neuroprotective Action of Humanin and Humanin Analogues

Comprehensive review of humanin's neuroprotective effects in Alzheimer's and Parkinson's disease models.

n.d.

Original Discovery of Humanin

Hashimoto et al. cloned humanin from Alzheimer's patient brain; demonstrated protection against amyloid-β toxicity.

n.d.

The mitochondrial derived peptide humanin is a regulator of lifespan and healthspan

Humanin overexpression extends lifespan in C. elegans; HNG treatment improves metabolic healthspan in middle-aged mice; levels higher in centenarian offspring.

n.d.

Mitochondria-derived peptides in aging and healthspan

Review of mitochondria-derived peptides including humanin and their roles in aging and metabolic health.

n.d.

Frequently Asked Questions About Humanin

Straight answers on reconstitution, dosing, and safety, everything you need to research with confidence. For research reference only.