Cognitive
Humanin
Longevity
24
Amino acids
Molecular weight
Peptide
Type
Humanin is a 24-amino acid mitochondrial-derived peptide encoded by the MT-RNR2 gene within the 16S ribosomal RNA region of mitochondrial DNA. It exerts cytoprotective, anti-apoptotic, and neuroprotective effects through JAK/STAT3 and PI3K/Akt signaling pathways, and is strongly associated with longevity — circulating levels are significantly higher in centenarians and long-lived animal models. Researchers and longevity-focused clinicians explore it for its potential to protect neurons, preserve metabolic health, and slow cellular aging.
Top researched benefits
Overview of Humanin
Humanin binds a trimeric receptor complex (CNTFR/WSX-1/gp130) to activate STAT3, PI3K/Akt, and MAPK signaling cascades that suppress apoptosis and oxidative stress. Intracellularly, it localizes to mitochondria where it inhibits reactive oxygen species formation and restores mitochondrial bioenergetics, blocking cell senescence and programmed death.
neuroprotection
- Originally discovered for its ability to protect against amyloid-β toxicity in neuronal cells.
- Promotes mitochondrial biogenesis via PI3K/AKT signaling in PD models.
- Broad neuroprotective effects against various neurotoxic insults.
longevity
- Overexpression increases lifespan in C. elegans, dependent on daf-16/Foxo pathway.
- HNG analog improves metabolic healthspan parameters in middle-aged mice.
- Higher levels associated with longevity in centenarians and their offspring.
cellular protection
- Protects various cell types from stress-induced damage and death.
- Blocks pro-apoptotic proteins like Bax to prevent programmed cell death.
- Promotes mitochondrial function and biogenesis.
Typical Dose
2–5mg per injection
Frequency
2–3 times per week
Cycle Duration
2–4 weeks on, 2–4 weeks off
Storage
Lyophilized: -20°C for long-term, short-term at 4°C. Reconstituted: 2–8°C for up to 30–60 days; protect from light and repeated freeze-thaw cycles.
Chemical Makeup
Key benefits
Protects neurons from beta-amyloid-induced toxicity, offering a potential therapeutic avenue in Alzheimer's and Parkinson's disease models
Reduces infarct size and preserves cardiac function following ischemic injury by attenuating cardiomyocyte apoptosis
Improves glucose metabolism and insulin sensitivity in animal models by enhancing mitochondrial bioenergetics
Suppresses pro-inflammatory cytokines including TNF-α and IL-6, reducing systemic and neuroinflammation
Community interest
This peptide is still gaining traction in the community.
Mitochondrial Peptide | Neuroprotection & Longevity
This overview is informational and based on aggregated descriptions from studies and user reports.
Was it helpful?YesNoHumanin Molecular Information
View the scientifc details of Humanin.
24
Amino Acids
Humanin
Met
Met
Position 1
Ala
Ala
Position 2
Pro
Pro
Position 3
Arg
Arg
Position 4
Gly
Gly
Position 5
Phe
Phe
Position 6
Ser
Ser
Position 7
Cys
Cys
Position 8
Leu
Leu
Position 9
Leu
Leu
Position 10
Leu
Leu
Position 11
Leu
Leu
Position 12
Thr
Thr
Position 13
Ser
Ser
Position 14
Glu
Glu
Position 15
Ile
Ile
Position 16
Asp
Asp
Position 17
Leu
Leu
Position 18
Pro
Pro
Position 19
Val
Val
Position 20
Lys
Lys
Position 21
Arg
Arg
Position 22
Arg
Arg
Position 23
Ala
Ala
Position 24
Molecular Weight
2687.28DaChain Length
24Amino AcidsType
PeptideHumanin Protocols
Subcutaneous injection is the primary and best-documented route for Humanin, providing gradual systemic absorption from the injection depot. Because Humanin has a short plasma half-life (approximately 30 minutes in animal models), morning administration is preferred to coincide with the circadian peak in mitochondrial biogenesis signaling, maximizing daytime cellular energy support.
| Goal | Dosage | Frequency | Route |
|---|---|---|---|
| Longevity and healthspan extension | 5 | 1 day range | SubQ |
| Anti-aging intensive cycle | 10 | 1 day range | SubQ |
| General health optimization | 5 | 3 week range | SubQ |
| Metabolic health and insulin sensitivity | 2 | 3 week range | SubQ |
| Cardiac ischemia-reperfusion protection (preclinical) | 2 | 1 day range | SubQ or IV |
| Neuroprotection | 5 | 2 week range | SubQ |
Reconstitution Instructions
Materials needed:
Steps to reconstitute
- Remove the Humanin vial from the freezer and allow it to reach room temperature for 10–15 minutes before reconstitution
- Using an alcohol swab, clean the rubber stoppers of both the Humanin vial and the bacteriostatic water (BAC water) vial
- Draw the desired volume of BAC water into an insulin syringe — for a 10 mg vial, use 1–2 mL BAC water to achieve a concentration of 5–10 mg/mL
- Insert the needle into the Humanin vial and slowly inject BAC water down the inside wall of the vial — never inject directly onto the powder
- Gently swirl the vial to dissolve the powder — do not shake, vortex, or expose to heat
- Allow the solution to sit for 1–2 minutes until completely clear before drawing your dose
- Draw the calculated dose volume into a fresh insulin syringe and remove any air bubbles
- Wipe the injection site (abdomen, thigh) with an alcohol swab and allow to dry
- Pinch the skin, insert the needle at a 45-degree angle into subcutaneous fat, and inject slowly
- Store reconstituted vial in the refrigerator at 2–8°C and use within 30 days
Humanin Cycle
The Humanin Cycle section explains how long a typical cycle lasts and what to expect during each phase. Over time, your body can become less responsive with continuous use.
Taking breaks between cycles may help maintain effectiveness and support better overall results.
- Week 1-2
- Subtle; peptide begins working at cellular level
- Week 2-4
- Improved energy and reduced fatigue may begin
- Week 4-8
- Enhanced cellular resilience and stress response
- Week 8-12
- Cumulative benefits in mitochondrial function and healthspan markers
- Week 0-0
- Potential longevity and neuroprotective benefits
Dosing tools
Humanin Peptide Dosage Calculator
Calculate peptide doses with our visual syringe guide.
mg
Enter the total amount of peptide in the vial in milligrams (as stated on the label).
The dose you want to inject per administration, in mcg or mg.
1,000 mcg = 1 mg
1 mL
2 mL
3 mL
5 mL
Custom
Volume of bacteriostatic water you add to reconstitute the powder. Use BAC water for preservation.
Injection Results
Based on your vial and dilution inputs.
Humanin
SINGLE COMPOUNDVolume per injection
0.05
mL
Concentration
10.00
mg/mL
Doses per vial
20
doses
Total injections per vial
20 injections
How it works
Based on a 10 mg Humanin vial diluted with 1 mL of bacteriostatic water, each 500 mcg injection equals 0.05 mL.
1mL / 100 units
5 units
0.050 mL
Reference Guide
Dosing Cycle
- Peptide
- Humanin
- Dosing
- 2–5mg per injection
- Dosing Frequency
- 2–3 times per week
- Cycle Duration
- 2–4 weeks on, 2–4 weeks off
- Storage
- Lyophilized: -20°C for long-term, short-term at 4°C. Reconstituted: 2–8°C for up to 30–60 days; protect from light and repeated freeze-thaw cycles.
Note: Triple agonist; microdose for fewer side effects
Reconstitution Tips
- Use bacteriostatic water (BAC) — contains 0.9% benzyl alcohol for preservation
- Inject water slowly — aim down the vial wall, not directly onto powder
- Never shake — gently swirl or roll the vial until dissolved
- Store properly — refrigerate at 2-8°C after reconstitution
- Use within 28 days — most reconstituted peptides remain stable for about 4 weeks
- Keep sterile — always clean vial tops with alcohol before drawing
Peptide Interactions
Research suggestions of Humanin interactions with other common peptides and substances.
Longevity
epi
Immunity
Epitalon
COMPATIBLE
Different mechanisms; can be combined in longevity protocols.
Longevity
mot
Metabolic
MOTS-c
SYNERGISTIC
Both are mitochondria-derived peptides with complementary longevity and metabolic benefits.
Longevity
nad
Metabolic
NAD+
SYNERGISTIC
Both support mitochondrial function and healthy aging.
Longevity
ss3
Metabolic
SS-31
SYNERGISTIC
Both target mitochondrial function; may have additive benefits for cellular health.
Side effects
Contraindications
Active cancer (theoretical concern with anti-apoptotic effects)
Pregnancy or breastfeeding
Hypersensitivity to peptide components
Stop signs
Signs of allergic reaction
Unusual persistent symptoms
Bad signs
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Frequently asked questions
What is a peptide dosage calculator?
A peptide dosage calculator is a free tool that converts your vial size, bacteriostatic water volume, and target dose into an exact syringe draw volume. Instead of doing the reconstitution math by hand, you enter three inputs and instantly get the concentration of your solution and how many milliliters or syringeunits to draw. This calculator works for single peptide compounds and multi-peptide blends.
How do I calculate peptide dosage from a vial?
To calculate your peptide dose, divide the total peptide content of your vial in micrograms by the volume of bacteriostatic water you added in milliliters. This gives you your solution concentration in mcg/mL. Then divide your target dose by that concentration to get your draw volume. For example, a 5mg (5,000 mcg) vial reconstituted with 2mL of BAC water gives a concentration of 2,500 mcg/mL. A 250 mcg dose would require drawing 0.1mL. This calculator automates all of those steps instantly.
How much Bacteriostatic water should I add to a peptide vial?
Most people add 2mL to 3mL of bacteriostatic water per vial, but the right amount depends on the dose you want to draw and the syringe size you are using. Adding 1mL to a 5mg vial gives you a concentration of 5,000 mcg/mL, making each dose very small in volume. Adding 2mL gives you 2,500 mcg/mL, which is easier to measure on a standard insulin syringe. A general guideline is to choose a volume that puts your typical dose somewhere between 10 and 30 units on a U-100 syringe. Use the calculator above to test different water volumes and find what works for your dose.
How are peptides different from proteins?
Both are made of amino acids, but peptides are much smaller than proteins. Because of their tiny size, peptides can act like tiny messengers in the body, sending specific signals to your cells to tell them exactly what to do.
HumaninResearch References
Humanin is a preclinical compound
Humanin
Humanin is a preclinical compound
The mitochondrial derived peptide humanin is a regulator of lifespan and healthspan
Humanin overexpression extends lifespan in C. elegans; HNG treatment improves metabolic healthspan in middle-aged mice; levels higher in centenarian offspring.
n.d.
Neuroprotective Action of Humanin and Humanin Analogues
Comprehensive review of humanin's neuroprotective effects in Alzheimer's and Parkinson's disease models.
n.d.
Mitochondria-derived peptides in aging and healthspan
Review of mitochondria-derived peptides including humanin and their roles in aging and metabolic health.
n.d.
Original Discovery of Humanin
Hashimoto et al. cloned humanin from Alzheimer's patient brain; demonstrated protection against amyloid-β toxicity.
n.d.
Calculate peptide dosages
Learning how to calculate a peptide dose? Use our beginner-friendly peptide dosage, blend, and accumulation calculators. Enter vial size, reconstitution volume, and target dose to get exact draw volumes instantly. No guesswork, just clear guidance that helps prevent common mistakes.
