Longevity
Thymulin
Immunity
1
Amino acids
Molecular weight
Peptide
Type
Thymulin is a zinc-dependent nonapeptide hormone secreted by thymic epithelial cells that plays a central role in T-cell maturation and immune regulation. It promotes differentiation of immature lymphocyte precursors into functional T-cells and modulates inflammatory cytokine signaling via the NF-κB and p38 MAPK pathways. Researchers and clinicians use it to address age-related immune decline, as thymulin levels fall by up to 90% between youth and age 60.
Top researched benefits
Overview of Thymulin
Thymulin binds to T-cell precursors as an active zinc-thymulin complex, driving their maturation into cytotoxic and helper T-cells and enhancing NK cell activity. It also downregulates NF-κB and p38 MAPK signaling in macrophages, reducing TNF-α and other pro-inflammatory cytokine output.
immune system research
- Age-related decline in thymulin correlates with reduced immune function; supplementation may restore T-cell parameters.
- Promotes differentiation and maturation of T-lymphocytes in thymus.
- Thymulin activity depends on zinc; studied in zinc-depleted conditions.
inflammatory and autoimmune research
- Research shows thymulin suppresses pro-inflammatory cytokines and mediators.
- Investigated for potential to restore immune balance in autoimmune states.
- Studied for effects on pancreatic beta cells and immune modulation in diabetes models.
neuroendocrine research
- Thymulin influences hypothalamic-pituitary-adrenal axis function.
- Some research suggests protective effects on neural tissue.
Typical Dose
2mg per injection
Frequency
once daily (evening)
Cycle Duration
20 consecutive days, 3 cycles per year (every ~4 months)
Storage
Lyophilized: store at -20°C, desiccated and protected from light, stable up to 24 months. Reconstituted: refrigerate at 2-8°C and use within 28 days; avoid freeze-thaw cycles.
Chemical Makeup
Key benefits
Drives maturation of immature lymphocyte precursors into functional CD4+ and CD8+ T-cells, directly restoring adaptive immune capacity
Enhances cytotoxic activity of NK cells, improving surveillance against virally infected and malignant cells
Reduces TNF-α and pro-inflammatory cytokine output from macrophages by suppressing NF-κB and p38 MAPK signaling pathways
Counteracts age-related thymic involution, partially restoring thymulin levels that decline by up to 90% by age 60
Community interest
This peptide is still gaining traction in the community.
Thymic Nonapeptide | Immune Restoration & Anti-Aging
This overview is informational and based on aggregated descriptions from studies and user reports.
Was it helpful?YesNoThymulin Molecular Information
View the scientifc details of Thymulin.
1
Amino Acids
Thymulin
Ala
Ala
Position 1
Molecular Weight
858.86DaChain Length
1Amino AcidsType
PeptideThymulin Protocols
Subcutaneous injection is the established route for thymulin (FTS), administered in the evening to align with the peptide's natural circadian thymic hormone rhythm. Due to thymulin's short serum half-life, intensive short-course protocols are required to drive T-cell maturation and immune reconstitution.
| Goal | Dosage | Frequency | Route |
|---|---|---|---|
| Restore T-cell populations and immune competence using an intensive daily course, repeated 3 times per year | 2000 | 1 day range | SubQ |
| Treat active immunity disorders using the manufacturer-recommended course of 5–20 mg daily for 3–10 days, via SubQ or IM | 5000 | 1 day range | SubQ or IM |
| Maintain baseline immune function with a 5-day preventive course every 6 months at a conservative dose | 5000 | 1 day range | SubQ |
| Initiate thymic T-cell education in new users with a low mcg-range starting dose to assess tolerance | 100 | 1 day range | SubQ |
| Sustain daily T-cell maturation signaling at the standard mcg-range dose throughout an ongoing course | 250 | 1 day range | SubQ |
| Support thymic rejuvenation and age-related immune decline using a combination protocol with epitalon, repeated every 6 months | 5000 | 1 day range | SubQ |
Reconstitution Instructions
Materials needed:
Steps to reconstitute
- Remove the thymulin vial from refrigerator and allow it to reach room temperature for 10–15 minutes before reconstitution.
- Clean the vial stopper with a fresh alcohol pad and allow to air dry for 30 seconds.
- Draw the desired volume of bacteriostatic water into a syringe — for a 20 mg vial, draw 2 mL BAC water to yield 10 mg/mL (10000 mcg/mL) concentration.
- Inject the BAC water slowly down the inside wall of the vial — never directly onto the powder.
- Swirl the vial gently until the powder is fully dissolved — do not shake or vortex.
- Store reconstituted vial in the refrigerator at 2–8°C; use within 28 days.
- Calculate draw volume: desired dose mcg ÷ concentration mcg/mL = mL to draw (e.g. 2000 mcg ÷ 10000 mcg/mL = 0.2 mL).
- Clean the injection site (abdomen at least 2 inches from navel, outer thigh, upper arm, or lower back above buttocks) with an alcohol pad.
- Pinch skin and insert the insulin syringe needle at a 45–90 degree angle; inject slowly and steadily.
- Withdraw needle, apply gentle pressure with gauze, and dispose of syringe in a sharps container. Rotate injection sites daily.
Thymulin Cycle
The Thymulin Cycle section explains how long a typical cycle lasts and what to expect during each phase. Over time, your body can become less responsive with continuous use.
Taking breaks between cycles may help maintain effectiveness and support better overall results.
- Week 0-0
- Receptor binding and initial cellular signaling
- Day 1-7
- Immune cell modulation begins; cytokine profile changes
- Week 2-4
- T-cell differentiation effects; measurable immune parameters
- Week 0-0
- Long-term immune restoration in research models
Dosing tools
Thymulin Peptide Dosage Calculator
Calculate peptide doses with our visual syringe guide.
mg
Enter the total amount of peptide in the vial in milligrams (as stated on the label).
The dose you want to inject per administration, in mcg or mg.
1,000 mcg = 1 mg
1 mL
2 mL
3 mL
5 mL
Custom
Volume of bacteriostatic water you add to reconstitute the powder. Use BAC water for preservation.
Injection Results
Based on your vial and dilution inputs.
Thymulin
SINGLE COMPOUNDVolume per injection
0.05
mL
Concentration
10.00
mg/mL
Doses per vial
20
doses
Total injections per vial
20 injections
How it works
Based on a 10 mg Thymulin vial diluted with 1 mL of bacteriostatic water, each 500 mcg injection equals 0.05 mL.
1mL / 100 units
5 units
0.050 mL
Reference Guide
Dosing Cycle
- Peptide
- Thymulin
- Dosing
- 2mg per injection
- Dosing Frequency
- once daily (evening)
- Cycle Duration
- 20 consecutive days, 3 cycles per year (every ~4 months)
- Storage
- Lyophilized: store at -20°C, desiccated and protected from light, stable up to 24 months. Reconstituted: refrigerate at 2-8°C and use within 28 days; avoid freeze-thaw cycles.
Note: Triple agonist; microdose for fewer side effects
Reconstitution Tips
- Use bacteriostatic water (BAC) — contains 0.9% benzyl alcohol for preservation
- Inject water slowly — aim down the vial wall, not directly onto powder
- Never shake — gently swirl or roll the vial until dissolved
- Store properly — refrigerate at 2-8°C after reconstitution
- Use within 28 days — most reconstituted peptides remain stable for about 4 weeks
- Keep sterile — always clean vial tops with alcohol before drawing
Peptide Interactions
Research suggestions of Thymulin interactions with other common peptides and substances.
Longevity
epi
Immunity
Epitalon
COMPATIBLE
Different mechanisms; no known negative interactions.
Longevity
thy
Immunity
Thymalin
COMPATIBLE
Thymalin is a peptide mixture while thymulin is a single defined peptide; can complement each other.
Side effects
Contraindications
Autoimmune diseases (use with medical supervision)
Organ transplant recipients on immunosuppression
Pregnancy or breastfeeding
Known hypersensitivity to thymic peptides
Stop signs
Signs of allergic reaction
Unexpected immune changes
Bad signs
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Frequently asked questions
What is a peptide dosage calculator?
A peptide dosage calculator is a free tool that converts your vial size, bacteriostatic water volume, and target dose into an exact syringe draw volume. Instead of doing the reconstitution math by hand, you enter three inputs and instantly get the concentration of your solution and how many milliliters or syringeunits to draw. This calculator works for single peptide compounds and multi-peptide blends.
How do I calculate peptide dosage from a vial?
To calculate your peptide dose, divide the total peptide content of your vial in micrograms by the volume of bacteriostatic water you added in milliliters. This gives you your solution concentration in mcg/mL. Then divide your target dose by that concentration to get your draw volume. For example, a 5mg (5,000 mcg) vial reconstituted with 2mL of BAC water gives a concentration of 2,500 mcg/mL. A 250 mcg dose would require drawing 0.1mL. This calculator automates all of those steps instantly.
How much Bacteriostatic water should I add to a peptide vial?
Most people add 2mL to 3mL of bacteriostatic water per vial, but the right amount depends on the dose you want to draw and the syringe size you are using. Adding 1mL to a 5mg vial gives you a concentration of 5,000 mcg/mL, making each dose very small in volume. Adding 2mL gives you 2,500 mcg/mL, which is easier to measure on a standard insulin syringe. A general guideline is to choose a volume that puts your typical dose somewhere between 10 and 30 units on a U-100 syringe. Use the calculator above to test different water volumes and find what works for your dose.
How are peptides different from proteins?
Both are made of amino acids, but peptides are much smaller than proteins. Because of their tiny size, peptides can act like tiny messengers in the body, sending specific signals to your cells to tell them exactly what to do.
ThymulinResearch References
Thymulin is a preclinical compound
Thymulin
Thymulin is a preclinical compound
Thymulin (FTS) - A Thymic Hormone
Comprehensive review of thymulin discovery, structure, and immunological functions.
n.d.
The neuroendocrine role of thymulin
Thymulin modulates hypothalamic-pituitary-adrenal axis and has neuroendocrine effects beyond immunity.
n.d.
Zinc and thymulin
Zinc is essential for thymulin biological activity; zinc deficiency causes functional thymulin deficiency.
n.d.
Thymulin and aging
Serum thymulin levels decline dramatically with age, correlating with immunosenescence and increased infection susceptibility.
n.d.
Calculate peptide dosages
Learning how to calculate a peptide dose? Use our beginner-friendly peptide dosage, blend, and accumulation calculators. Enter vial size, reconstitution volume, and target dose to get exact draw volumes instantly. No guesswork, just clear guidance that helps prevent common mistakes.
