Reviewed by the PeptideMind Team · Updated August 5, 2026

Thymosin Beta-4 (TB4) Dosage Guide, Benefits & Side Effects

Thymosin Beta-4 (TB4) dosage guide: typical protocols, injection tips, and side effects for wound healing, tissue repair, and recovery, backed by clinical research.

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Thymosin Beta-4 Dosage Schedule

A twice weekly protocol that helps the body heal tissue faster, calm inflammation, and repair muscles, tendons, ligaments, and skin. A popular choice for recovering from injuries and wounds.

Thymosin Beta-4 for Faster Tissue Repair

5 weeks, focused on healing and recovery.

2 days per week

Taken Monday and Thursday.

2 mg per injection

2 mg per injection, given just under the skin in the abdomen or thigh.

Thymosin Beta-4 (also called TB-500) is a peptide that helps the body heal tissue faster, calm inflammation, and repair muscles, tendons, ligaments, and skin. It's popular for recovering from injuries and wounds.

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What is Thymosin Beta-4?

Thymosin Beta-4 is a naturally occurring peptide found throughout the body that plays a central role in how cells move and rebuild themselves. It supports wound healing, helps form new blood vessels, and can mobilize stem cells to injury sites. It has reached late-stage human trials for a serious eye condition and earlier trials for skin wounds and heart repair, and it's also widely used in research and biohacking for injury recovery and reducing inflammation.

Healing

Research targets include tissue repair, wound healing, and recovery from injury.

Longevity

Research targets include cellular aging, senescence, and long-term healthspan.

Immunity

Research targets include immune cell activity, inflammatory response, and infection defense.

Healing

thymos

Longevity

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What Does Thymosin Beta-4 Do?

Inside cells, it helps manage the internal scaffolding protein actin that lets cells move; outside cells, it acts more like a hormone, switching on a pathway that drives cell migration, growth, and survival. This combination helps mobilize the body's own repair cells, encourage new blood vessel growth, and limit the kind of scarring that can follow an injury.

Manages cell scaffolding

Helps manage the internal actin scaffolding that lets cells move.

Acts like a hormone

Outside cells, switches on a pathway that drives migration, growth, and survival.

Limits scarring

Mobilizes repair cells, encourages new vessels, and limits scarring.

Thymosin Beta-4 Targeted areas

Frequently Asked Questions About Thymosin Beta-4

Straight answers on reconstitution, dosing, and safety, everything you need to research with confidence. For research reference only.

Thymosin Beta-4 Reconstitution Guide

Thymosin Beta-4 is supplied as a freeze-dried powder that must be mixed into a injectable solution before use. Available in 2mg, 5mg, 10mg sizes.

5 mg lyophilized vial

Thymosin Beta-4 is supplied as a freeze-dried powder that must be mixed into a injectable solution before use. Available in 2mg, 5mg, 10mg sizes.

2 mL bacteriostatic water

Adding 2 mL of bac water to a 5 mg vial gives a workable concentration for syringe dosing. More water makes microdosing easier; less concentrates each draw.

Storage after mixing

Freeze the dry powder, keeping it dry; it can handle room temperature for a few weeks. Once mixed with bacteriostatic water, refrigerate and use within about a month, avoiding repeated freezing and thawing. After reconstitution, keep the vial refrigerated and use within 30 days.

Reconstitution steps

1

Remove caps & sanitize stoppers

2

Attach needle to syringe

3

Draw 2 mL bacteriostatic water

4

Insert needle into Thymosin Beta-4 vial

5

Release water gently down the side

6

Swirl to dissolve — don't shake

7

Check for full dissolution

8

Refrigerate Thymosin Beta-4 — good for 30 days

Chemical Makeup

37

Amino Acids

Thymosin Beta-4

Lys

K

Position 1

Trp

W

Position 2

Lys

K

Position 3

Leu

L

Position 4

Phe

F

Position 5

Lys

K

Position 6

Lys

K

Position 7

Ile

I

Position 8

Glu

E

Position 9

Lys

K

Position 10

Val

V

Position 11

Gly

G

Position 12

Gln

Q

Position 13

Asn

N

Position 14

Ile

I

Position 15

Arg

R

Position 16

Asp

D

Position 17

Gly

G

Position 18

Ile

I

Position 19

Ile

I

Position 20

Lys

K

Position 21

Ala

A

Position 22

Gly

G

Position 23

Pro

P

Position 24

Ala

A

Position 25

Val

V

Position 26

Ala

A

Position 27

Val

V

Position 28

Val

V

Position 29

Gly

G

Position 30

Gln

Q

Position 31

Ala

A

Position 32

Thr

T

Position 33

Gln

Q

Position 34

Ile

I

Position 35

Ala

A

Position 36

Lys

K

Position 37

Amino acid sequence
LysinePosition 1
TryptophanPosition 2
LysinePosition 3
LeucinePosition 4
PhenylalaninePosition 5
LysinePosition 6
LysinePosition 7
IsoleucinePosition 8
Glutamic acidPosition 9
LysinePosition 10
ValinePosition 11
GlycinePosition 12
GlutaminePosition 13
AsparaginePosition 14
IsoleucinePosition 15
ArgininePosition 16
Aspartic acidPosition 17
GlycinePosition 18
IsoleucinePosition 19
IsoleucinePosition 20
LysinePosition 21
AlaninePosition 22
GlycinePosition 23
ProlinePosition 24
AlaninePosition 25
ValinePosition 26
AlaninePosition 27
ValinePosition 28
ValinePosition 29
GlycinePosition 30
GlutaminePosition 31
AlaninePosition 32
ThreoninePosition 33
GlutaminePosition 34
IsoleucinePosition 35
AlaninePosition 36
LysinePosition 37

What Are the Benefits of Thymosin Beta-4?

What research says it may help with, and how it works in the body.

tissue repair

It helps tissue regenerate better and shortens healing time for surgical wounds.

It speeds up recovery from injuries to muscles, tendons, and ligaments.

In clinical trials for pressure sores (bedsores), 67% of wounds fully healed compared to only 25% with a placebo.

cardiovascular

In mid-stage clinical trials, giving it within 6 hours of a heart attack reduced the amount of damaged heart tissue by 43%.

neuroprotection

In animal studies, it improved brain-related outcomes and helped restore normal function after injury.

What Are the Side Effects of Thymosin Beta-4?

Who should avoid it, warning signs to watch for, and what to know before combining it with other compounds.

Who Should Avoid It

Active chemotherapy treatment

Severe systemic allergies to peptides

Stop Right Away If You Notice

Severe injection site reactions or persistent inflammation

Signs of systemic allergic reaction (rash, breathing difficulty)

Unexpected cardiovascular symptoms during treatment

Any signs of infection at injection sites

Milder Signs to Watch For

Cloudy or discolored solution indicating degradationCrystallization after reconstitution suggesting improper storage
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Is Thymosin Beta-4 FDA approved?

Thymosin Beta-4 is not an FDA-approved drug. It is intended for research purposes only and is not approved for human consumption, diagnostic, or therapeutic use.

Research Use Only

Thymosin Beta-4 has not been evaluated by the FDA for safety or efficacy in humans.

No Clinical Oversight

Not manufactured under FDA-regulated quality or clinical standards.

Unregulated Sourcing

Purity, dosing, and sourcing are not verified through FDA testing or oversight.

A smartphone screen displaying FDA status for a drug, indicating "FDA approved drug: No" and "Classification: Research use only." A banner notes "Research Only" with the disclaimer "not for human use," emphasizing its intended use for laboratory and analytical purposes.

Thymosin Beta-4 Cycle Length: How Long Should It Last?

This breaks down how long a typical Thymosin Beta-4 cycle runs and what research suggests happens at each stage. Research shows that staying on a peptide continuously, without a break, may make it less effective over time.

That's why most research protocols build in a break between cycles, often called a washout period, to let the body reset before starting again.

Week 1-2
Initial tissue response, possible mild injection site reactions
Week 2-4
Accelerated healing becomes apparent, reduced inflammation
Week 4-8
Significant tissue regeneration and functional improvement
Week 8-12
Sustained benefits and near-complete healing in responsive conditions

Thymosin Beta-4 Research References

It is a phase 3 compound

4Research references

Thymosin Beta-4

Thymosin Beta-4 is a phase 3 compound

Phase II Dry Eye Clinical Trial

72 subjects showing significant positive effects on ocular discomfort and corneal staining with excellent safety profile.

2015

Cardiac Protection Pilot Study: Thymosin β4 in STEMI Patients

First human study in heart attack patients demonstrating potential clinical benefits in repairing damaged tissue.

2016

Neurotrophic Keratopathy Compassionate Use Trial

Complete clearing of persistent corneal defects in most subjects using topical formulation.

2010

Phase I Human Safety Study: Intravenous Thymosin β4

First-in-human study establishing safety and pharmacokinetics in healthy volunteers with no dose-limiting toxicity.

2010