Reviewed by the PeptideMind Team · Updated July 15, 2026

Thymosin Beta-4 Dosage Guide, Benefits & Side Effects

Thymosin Beta-4 speeds wound closure and angiogenesis while limiting cardiac infarct size, with Phase 3 data on corneal repair too.

Chemical Makeup

37

Amino Acids

Thymosin Beta-4

Lys

K

Position 1

Trp

W

Position 2

Lys

K

Position 3

Leu

L

Position 4

Phe

F

Position 5

Lys

K

Position 6

Lys

K

Position 7

Ile

I

Position 8

Glu

E

Position 9

Lys

K

Position 10

Val

V

Position 11

Gly

G

Position 12

Gln

Q

Position 13

Asn

N

Position 14

Ile

I

Position 15

Arg

R

Position 16

Asp

D

Position 17

Gly

G

Position 18

Ile

I

Position 19

Ile

I

Position 20

Lys

K

Position 21

Ala

A

Position 22

Gly

G

Position 23

Pro

P

Position 24

Ala

A

Position 25

Val

V

Position 26

Ala

A

Position 27

Val

V

Position 28

Val

V

Position 29

Gly

G

Position 30

Gln

Q

Position 31

Ala

A

Position 32

Thr

T

Position 33

Gln

Q

Position 34

Ile

I

Position 35

Ala

A

Position 36

Lys

K

Position 37

Amino acid sequence
LysinePosition 1
TryptophanPosition 2
LysinePosition 3
LeucinePosition 4
PhenylalaninePosition 5
LysinePosition 6
LysinePosition 7
IsoleucinePosition 8
Glutamic acidPosition 9
LysinePosition 10
ValinePosition 11
GlycinePosition 12
GlutaminePosition 13
AsparaginePosition 14
IsoleucinePosition 15
ArgininePosition 16
Aspartic acidPosition 17
GlycinePosition 18
IsoleucinePosition 19
IsoleucinePosition 20
LysinePosition 21
AlaninePosition 22
GlycinePosition 23
ProlinePosition 24
AlaninePosition 25
ValinePosition 26
AlaninePosition 27
ValinePosition 28
ValinePosition 29
GlycinePosition 30
GlutaminePosition 31
AlaninePosition 32
ThreoninePosition 33
GlutaminePosition 34
IsoleucinePosition 35
AlaninePosition 36
LysinePosition 37

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Thymosin Beta-4 Dosage Guide for Recovery & Repair

Thymosin Beta-4 (also called TB-500) is a peptide that helps the body heal tissue faster, calm inflammation, and repair muscles, tendons, ligaments, and skin. It's popular for recovering from injuries and wounds.

Vial size

5 mg

Bacteriostatic water

2 mL

Dosing

1–2.5 mg

Frequency

2×/week

Cycle

4–6 weeks

Benefit

Recovery & Repair

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What is Thymosin Beta-4?

Thymosin Beta-4 is a naturally occurring peptide found throughout the body that plays a central role in how cells move and rebuild themselves. It supports wound healing, helps form new blood vessels, and can mobilize stem cells to injury sites. It has reached late-stage human trials for a serious eye condition and earlier trials for skin wounds and heart repair, and it's also widely used in research and biohacking for injury recovery and reducing inflammation.

Healing

Research targets include tissue repair, wound healing, and recovery from injury.

Longevity

Research targets include cellular aging, senescence, and long-term healthspan.

Immunity

Research targets include immune cell activity, inflammatory response, and infection defense.

Healing

thymos

Longevity

Is Thymosin Beta-4 FDA approved?

Thymosin Beta-4 is not an FDA-approved drug. It is intended for research purposes only and is not approved for human consumption, diagnostic, or therapeutic use.

Research Use Only

Thymosin Beta-4 has not been evaluated by the FDA for safety or efficacy in humans.

No Clinical Oversight

Not manufactured under FDA-regulated quality or clinical standards.

Unregulated Sourcing

Purity, dosing, and sourcing are not verified through FDA testing or oversight.

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How Does Thymosin Beta-4 Work?

Inside cells, it helps manage the internal scaffolding protein actin that lets cells move; outside cells, it acts more like a hormone, switching on a pathway that drives cell migration, growth, and survival. This combination helps mobilize the body's own repair cells, encourage new blood vessel growth, and limit the kind of scarring that can follow an injury.

Manages cell scaffolding

Helps manage the internal actin scaffolding that lets cells move.

Acts like a hormone

Outside cells, switches on a pathway that drives migration, growth, and survival.

Limits scarring

Mobilizes repair cells, encourages new vessels, and limits scarring.

Thymosin Beta-4 Targeted areas

How to reconstitute Thymosin Beta-4

View guide

The materials you'll need and step-by-step instructions for safely mixing Thymosin Beta-4 with bacteriostatic water.

Materials needed

Your Thymosin Beta-4 vial (lyophilized)

Alcohol swabs

Bacteriostatic sterile water

3 mL syringes (Luer Lock tip)

25G or 27G needles (Luer Lock). Other gauges may also be acceptable.

Sharps container (optional)

1

Remove the caps

Remove the caps from both your Thymosin Beta-4 vial and your bacteriostatic water vial.
2

Sanitize the rubber stoppers

Wipe down the rubber stoppers on both vials with alcohol swabs and let them dry for about 3 minutes.
3

Attach the needle

Take your 3 mL syringe and needle. Twist the needle's plastic hub into the Luer Lock tip on the syringe to secure it.
4

Draw the bac water

Pull back on the plunger to draw your desired volume of bacteriostatic water. If you overfill, just push the excess back in until you reach the right marker on the syringe.

How much bac water? Thymosin Beta-4 typically comes in a 5 mg vial. We recommend 2 mL of bacteriostatic water, though anywhere between 1 mL and 3 mL is acceptable. For larger vials you can use up to 5 mL — more bac water makes microdosing easier, but insulin needles usually max out at 1 mL per injection. That means a lot of bac water might force you into multiple injections to hit your target dose. Unless you're microdosing, 2 mL per vial is a solid rule of thumb.
5

Insert the needle into the Thymosin Beta-4 vial

With the bac water in your syringe, insert the needle into the Thymosin Beta-4 vial at a slight angle to avoid pressure buildup.

Use a thin needle: Stick to 25G or higher to avoid damaging the rubber stopper. Higher gauge means a thinner needle.
6

Release the water gently

Let the water run gently down the side of the vial. Don't inject it forcefully.

Heads up: Sometimes the plunger pushes itself in as soon as the needle enters the vial. Hold the plunger back so you have full control over how fast the bac water enters.
7

Swirl to dissolve

Avoid shaking. Gently swirl, flip, and roll the vial to dissolve the powder.

Be patient: This usually takes 2 to 5 minutes of consistent swirling, mixing in a circular motion, and gently flipping the vial up and down. The water needs to run through the powder many times to fully dissolve it. Don't shake or get aggressive with it.
8

Check for full dissolution

The solution should be clear with no visible particles. If it's cloudy or clumpy, wait a minute and repeat the swirling step.
9

Cap, dispose, and store

Once you're done, cap the needle and dispose of it (ideally in a sharps container). Store the reconstituted Thymosin Beta-4 vial in the fridge whenever it's not in use. It will stay good for approximately 30 days.

How to Store Thymosin Beta-4

Freeze the dry powder, keeping it dry; it can handle room temperature for a few weeks. Once mixed with bacteriostatic water, refrigerate and use within about a month, avoiding repeated freezing and thawing.

Lyophilized Storage

-20°C, desiccated; stable at room temperature for up to 3 weeks.

Reconstituted Storage

With bacteriostatic water, 2–8°C for up to 28 days.

Handling Notes

Avoid freeze-thaw cycles.

A smartphone displaying a "Storage Guide" for peptides, indicating a safe storage temperature range of 2–8°C, with warnings to keep away from light, heat, and freezing. The background is light blue, and additional tips on protecting the peptides are visible below the temperature range.

What Are the Benefits of Thymosin Beta-4?

What research says it may help with, and how it works in the body.

tissue repair

It helps tissue regenerate better and shortens healing time for surgical wounds.

It speeds up recovery from injuries to muscles, tendons, and ligaments.

In clinical trials for pressure sores (bedsores), 67% of wounds fully healed compared to only 25% with a placebo.

cardiovascular

In mid-stage clinical trials, giving it within 6 hours of a heart attack reduced the amount of damaged heart tissue by 43%.

neuroprotection

In animal studies, it improved brain-related outcomes and helped restore normal function after injury.

What Are the Side Effects of Thymosin Beta-4?

Who should avoid it, warning signs to watch for, and what to know before combining it with other compounds.

Who Should Avoid It

Active chemotherapy treatment

Severe systemic allergies to peptides

Stop Right Away If You Notice

Severe injection site reactions or persistent inflammation

Signs of systemic allergic reaction (rash, breathing difficulty)

Unexpected cardiovascular symptoms during treatment

Any signs of infection at injection sites

Milder Signs to Watch For

Cloudy or discolored solution indicating degradationCrystallization after reconstitution suggesting improper storage
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How Long Should a Thymosin Beta-4 Cycle Last?

This breaks down how long a typical Thymosin Beta-4 cycle runs and what research suggests happens at each stage. Research shows that staying on a peptide continuously, without a break, may make it less effective over time.

That's why most research protocols build in a break between cycles, often called a washout period, to let the body reset before starting again.

Week 1-2
Initial tissue response, possible mild injection site reactions
Week 2-4
Accelerated healing becomes apparent, reduced inflammation
Week 4-8
Significant tissue regeneration and functional improvement
Week 8-12
Sustained benefits and near-complete healing in responsive conditions

Cheat Sheets Featuring Thymosin Beta-4

See how Thymosin Beta-4 fits into a broader research stack with these free downloadable cheat sheets. Each one includes reference dose ranges, dosing frequency, and half-life for every peptide it covers. For research purposes only.

View all cheat sheets
Healing & Recovery Peptide Cheat Sheet
Healing & Recovery Peptide Cheat Sheet

18 peptides

Immunity Peptide Cheat Sheet
Immunity Peptide Cheat Sheet

15 peptides

Longevity Peptide Cheat Sheet
Longevity Peptide Cheat Sheet

42 peptides

Master Peptide Cheat Sheet
Master Peptide Cheat Sheet

78 peptides

Thymosin Beta-4 Research References

It is a phase 3 compound

4Research references

Thymosin Beta-4

Thymosin Beta-4 is a phase 3 compound

Phase II Dry Eye Clinical Trial

72 subjects showing significant positive effects on ocular discomfort and corneal staining with excellent safety profile.

2015

Cardiac Protection Pilot Study: Thymosin β4 in STEMI Patients

First human study in heart attack patients demonstrating potential clinical benefits in repairing damaged tissue.

2016

Neurotrophic Keratopathy Compassionate Use Trial

Complete clearing of persistent corneal defects in most subjects using topical formulation.

2010

Phase I Human Safety Study: Intravenous Thymosin β4

First-in-human study establishing safety and pharmacokinetics in healthy volunteers with no dose-limiting toxicity.

2010

Frequently Asked Questions About Thymosin Beta-4

Straight answers on reconstitution, dosing, and safety, everything you need to research with confidence. For research reference only.