Longevity
Thymogen
Immunity
2
Amino acids
Molecular weight
Peptide
Type
Thymogen is a synthetic dipeptide (L-Glu-L-Trp) derived from thymic extracts, developed by Vladimir Khavinson as a targeted immunomodulator. It restores T-lymphocyte differentiation and cytokine balance by activating intracellular signaling cascades in immune cells. Approved in Russia as both an intranasal spray and injectable formulation, it is used clinically for immune dysfunction, post-surgical recovery, and age-related immunosenescence.
Top researched benefits
Overview of Thymogen
Thymogen binds to immune cell surface receptors and triggers an intracellular regulatory cascade that enhances transmembrane Ca2+ exchange in lymphocytes, shifting the cAMP/cGMP ratio and increasing protein kinase activity to stimulate T-cell differentiation, NK cell activation, and cytokine production. It also modulates gene expression governing cell proliferation, apoptosis, and heat-shock protein synthesis.
immune support
- Modulates both humoral and cellular immune responses for balanced immunity.
- Activates T-cell differentiation and normalizes CD3+, CD4+, CD8+ populations.
- Stimulates production of IgA, IgG, IgE, and IgM antibodies.
- Studied for improving surgical outcomes through immune support.
antiaging research
- Demonstrated geroprotector activity in aging research.
- In combination with pineal peptides, showed significant mean lifespan increases in animal models.
- Inhibits spontaneous carcinogenesis and shows robust antitumor effects in research.
clinical research areas
- Studied in Russia for regulating immune response in oncology patients.
- Research interest in cardiovascular applications.
- Investigated for metabolic and immune aspects of diabetes.
Typical Dose
100–1000mcg per dose
Frequency
once daily
Cycle Duration
3–10 day course, repeated as needed
Storage
Lyophilized: room temperature (15–25°C), stable up to 36 months. Reconstituted: 2–8°C for up to 28 days.
Chemical Makeup
Key benefits
Restores T-lymphocyte differentiation and maturation in immunocompromised patients, including those with post-surgical or chemotherapy-induced immunosuppression
Normalizes coagulation and fibrinolytic activity of blood plasma, reducing postoperative complications in elderly surgical patients
Reduces spontaneous tumor incidence and increases survival in animal carcinogenesis models via immune surveillance enhancement
Counteracts age-related thymic involution (immunosenescence) by restoring thymic peptide balance in older individuals
Community interest
This peptide is still gaining traction in the community.
Thymic Dipeptide | Immune Restoration & Anti-Aging
This overview is informational and based on aggregated descriptions from studies and user reports.
Was it helpful?YesNoThymogen Molecular Information
View the scientifc details of Thymogen.
2
Amino Acids
Thymogen
Glu
Glu
Position 1
Trp
Trp
Position 2
Molecular Weight
333.34DaChain Length
2Amino AcidsType
PeptideThymogen Protocols
Intramuscular injection is the primary clinical route for Thymogen, delivering the Glu-Trp dipeptide systemically for immune reconstitution and anti-infective support. Thymogen is supplied as a ready-to-use solution at 100 mcg/mL in 1 mL pre-filled ampoules — no reconstitution required. Administered once daily in the morning for 3–10 day courses, with up to four courses per year separated by at least one month.
| Goal | Dosage | Frequency | Route |
|---|---|---|---|
| Immune reconstitution / severe immunodeficiency | 100 | 1 day range | IM (deltoid or vastus lateralis) |
| Post-chemotherapy or radiation immune recovery | 100 | 1 day range | IM |
| Acute infection treatment | 100 | 1 day range | IM |
| Post-surgical immune support | 100 | 1 day range | IM |
| Age-related immune decline (anti-aging) | 100 | 1 day range | IM |
Reconstitution Instructions
Materials needed:
Steps to reconstitute
- Retrieve one Thymogen ampoule (100 mcg/mL, 1 mL) from refrigerated storage and allow it to reach room temperature for 5–10 minutes
- Inspect the ampoule for particulates or discoloration — discard if the solution is not clear and colorless
- Clean the ampoule neck with an alcohol swab and snap open at the scored line
- Draw the full 1 mL contents into a 1 mL syringe with a 25–27 gauge needle, yielding a single 100 mcg dose
- Choose an intramuscular injection site (deltoid or vastus lateralis), clean with an alcohol swab, and allow to dry for 30 seconds
- Insert the needle at a 90-degree angle into the muscle, aspirate briefly to confirm no blood return, then inject slowly
- Withdraw the needle and apply gentle pressure with a sterile gauze pad
- Discard the used syringe and open ampoule immediately — ampoules are single-use only
Thymogen Cycle
The Thymogen Cycle section explains how long a typical cycle lasts and what to expect during each phase. Over time, your body can become less responsive with continuous use.
Taking breaks between cycles may help maintain effectiveness and support better overall results.
- Week 0-0
- Rapid tissue distribution to thymus, lymph nodes, liver, kidneys
- Day 1-10
- T-cell activation and differentiation begins
- Week 0-0
- Immunoglobulin production stimulated; immune markers improve
- Week 0-0
- Effects persist due to cellular and epigenetic changes
- Week 0-0
- Cumulative geroprotective benefits with regular cycling
Dosing tools
Thymogen Peptide Dosage Calculator
Calculate peptide doses with our visual syringe guide.
mg
Enter the total amount of peptide in the vial in milligrams (as stated on the label).
The dose you want to inject per administration, in mcg or mg.
1,000 mcg = 1 mg
1 mL
2 mL
3 mL
5 mL
Custom
Volume of bacteriostatic water you add to reconstitute the powder. Use BAC water for preservation.
Injection Results
Based on your vial and dilution inputs.
Thymogen
SINGLE COMPOUNDVolume per injection
0.05
mL
Concentration
10.00
mg/mL
Doses per vial
20
doses
Total injections per vial
20 injections
How it works
Based on a 10 mg Thymogen vial diluted with 1 mL of bacteriostatic water, each 500 mcg injection equals 0.05 mL.
1mL / 100 units
5 units
0.050 mL
Reference Guide
Dosing Cycle
- Peptide
- Thymogen
- Dosing
- 100–1000mcg per dose
- Dosing Frequency
- once daily
- Cycle Duration
- 3–10 day course, repeated as needed
- Storage
- Lyophilized: room temperature (15–25°C), stable up to 36 months. Reconstituted: 2–8°C for up to 28 days.
Note: Triple agonist; microdose for fewer side effects
Reconstitution Tips
- Use bacteriostatic water (BAC) — contains 0.9% benzyl alcohol for preservation
- Inject water slowly — aim down the vial wall, not directly onto powder
- Never shake — gently swirl or roll the vial until dissolved
- Store properly — refrigerate at 2-8°C after reconstitution
- Use within 28 days — most reconstituted peptides remain stable for about 4 weeks
- Keep sterile — always clean vial tops with alcohol before drawing
Peptide Interactions
Research suggestions of Thymogen interactions with other common peptides and substances.
Longevity
epi
Immunity
Epitalon
SYNERGISTIC
Combination showed significant lifespan increases and robust antitumor activity in research.
Longevity
thy
Immunity
Thymalin
SYNERGISTIC
Thymogen was isolated from Thymalin; they share complementary thymus-supporting mechanisms.
Longevity
thymu
Immunity
Thymulin
COMPATIBLE
Different structures and mechanisms; can be used together.
Longevity
vil
Immunity
Vilon
SYNERGISTIC
Both are Khavinson thymus dipeptides; often combined in comprehensive immune protocols.
Side effects
Contraindications
Active autoimmune diseases (use with caution)
Organ transplant recipients on immunosuppression
Known hypersensitivity to component amino acids
Pregnancy or breastfeeding
Stop signs
Signs of allergic reaction
Unusual immune symptoms
Severe injection site reactions
Bad signs
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Frequently asked questions
What is a peptide dosage calculator?
A peptide dosage calculator is a free tool that converts your vial size, bacteriostatic water volume, and target dose into an exact syringe draw volume. Instead of doing the reconstitution math by hand, you enter three inputs and instantly get the concentration of your solution and how many milliliters or syringeunits to draw. This calculator works for single peptide compounds and multi-peptide blends.
How do I calculate peptide dosage from a vial?
To calculate your peptide dose, divide the total peptide content of your vial in micrograms by the volume of bacteriostatic water you added in milliliters. This gives you your solution concentration in mcg/mL. Then divide your target dose by that concentration to get your draw volume. For example, a 5mg (5,000 mcg) vial reconstituted with 2mL of BAC water gives a concentration of 2,500 mcg/mL. A 250 mcg dose would require drawing 0.1mL. This calculator automates all of those steps instantly.
How much Bacteriostatic water should I add to a peptide vial?
Most people add 2mL to 3mL of bacteriostatic water per vial, but the right amount depends on the dose you want to draw and the syringe size you are using. Adding 1mL to a 5mg vial gives you a concentration of 5,000 mcg/mL, making each dose very small in volume. Adding 2mL gives you 2,500 mcg/mL, which is easier to measure on a standard insulin syringe. A general guideline is to choose a volume that puts your typical dose somewhere between 10 and 30 units on a U-100 syringe. Use the calculator above to test different water volumes and find what works for your dose.
How are peptides different from proteins?
Both are made of amino acids, but peptides are much smaller than proteins. Because of their tiny size, peptides can act like tiny messengers in the body, sending specific signals to your cells to tell them exactly what to do.
ThymogenResearch References
Thymogen is an approved compound
Thymogen
Thymogen is an approved compound
Immunomodulatory synthetic dipeptide L-Glu-L-Trp slows down aging and inhibits spontaneous carcinogenesis in rats
Thymogen demonstrated geroprotector activity and inhibited spontaneous carcinogenesis in aging rat models.
n.d.
Natural and synthetic thymic peptides as therapeutics for immune dysfunction
Review of thymic peptide therapeutics including Thymogen for immune modulation.
n.d.
Peptide Bioregulators from Thymus and Pineal Gland
Significant lifespan increases and antitumor activity with combined Thymogen and Epitalon treatment.
n.d.
Thymogen: Mechanism of Action Studies
Thymogen activates T-cell differentiation, modulates cyclic nucleotides, and may interact with AACG DNA sequences.
n.d.
Calculate peptide dosages
Learning how to calculate a peptide dose? Use our beginner-friendly peptide dosage, blend, and accumulation calculators. Enter vial size, reconstitution volume, and target dose to get exact draw volumes instantly. No guesswork, just clear guidance that helps prevent common mistakes.
