Weight
CJC-1295 (without DAC)
Growth
29
Amino acids
Molecular weight
Peptide
Type
CJC-1295 without DAC (also known as Modified GRF 1-29) is a synthetic 29-amino acid analog of growth hormone-releasing hormone (GHRH) designed with four amino acid substitutions to resist enzymatic degradation. It stimulates the pituitary gland to release growth hormone in a pulsatile, physiologically natural pattern. It is widely used in longevity, body composition, and recovery research, often combined with a GHRP such as Ipamorelin.
Top researched benefits
Overview of CJC-1295 (without DAC)
CJC-1295 without DAC binds to the GHRH receptor (GHRHR) on somatotroph cells of the anterior pituitary, triggering a cAMP-mediated signaling cascade that drives pulsatile GH secretion and downstream IGF-1 production. Its D-Ala2, Gln8, Ala15, and Leu27 substitutions confer resistance to dipeptidylpeptidase-IV cleavage, extending activity relative to native GHRH(1-29).
growth hormone
- Restores natural GH pulsatility patterns in aging individuals.
- Elevates IGF-1 through enhanced GH secretion.
- Supports pituitary axis without suppression of natural function.
antiaging
- Supports maintenance of lean muscle mass through GH elevation.
- Supports bone health through GH-mediated pathways.
- Improvements in skin quality through collagen synthesis.
recovery
- Enhanced recovery from training through GH elevation.
- Improved sleep architecture with bedtime dosing.
Typical Dose
100–300mcg per injection
Frequency
once daily, before sleep or 30 minutes pre-workout
Cycle Duration
8–12 weeks on, 4 weeks off
Storage
Lyophilized: -20°C for up to 24–36 months. Reconstituted with bacteriostatic water: 2–8°C for up to 28 days. Do not freeze reconstituted solution.
Chemical Makeup
Key benefits
Stimulates pulsatile GH release from the anterior pituitary, producing 2–10 fold increases in plasma GH within hours of injection
Elevates IGF-1 levels by 1.5–3 fold for up to 9–11 days following a single dose, supporting muscle protein synthesis and tissue repair
Promotes lean muscle mass accrual and reduces adipose tissue through GH-driven lipolysis and anabolic signaling
Enhances slow-wave (deep) sleep quality, during which the majority of endogenous GH secretion naturally occurs
Community interest
This peptide is still gaining traction in the community.
GHRH Analog | GH Secretion & Anti-Aging
This overview is informational and based on aggregated descriptions from studies and user reports.
Was it helpful?YesNoCJC-1295 (without DAC) Molecular Information
View the scientifc details of CJC-1295 (without DAC).
29
Amino Acids
CJC-1295 (without DAC)
Tyr
Tyr
Position 1
Ala
Ala
Position 2
Asp
Asp
Position 3
Ala
Ala
Position 4
?
?
Position 5
Phe
Phe
Position 6
?
?
Position 7
Gln
Gln
Position 8
Ser
Ser
Position 9
Tyr
Tyr
Position 10
Arg
Arg
Position 11
Lys
Lys
Position 12
Val
Val
Position 13
Leu
Leu
Position 14
Ala
Ala
Position 15
Gln
Gln
Position 16
Leu
Leu
Position 17
Ser
Ser
Position 18
Ala
Ala
Position 19
Arg
Arg
Position 20
Lys
Lys
Position 21
Leu
Leu
Position 22
Leu
Leu
Position 23
Gln
Gln
Position 24
Asp
Asp
Position 25
?
?
Position 26
Leu
Leu
Position 27
Ser
Ser
Position 28
Arg
Arg
Position 29
Molecular Weight
3367.9DaChain Length
29Amino AcidsType
PeptideCJC-1295 (without DAC) Protocols
Subcutaneous injection is the only documented delivery route for CJC-1295 (without DAC). Its ~30-minute half-life produces a sharp, transient GH pulse that closely mimics the body's natural pulsatile GHRH signaling — making once-daily dosing appropriate for anti-aging and sleep goals, while performance-oriented protocols use two to three injections per day timed in the fasted state.
| Goal | Dosage | Frequency | Route |
|---|---|---|---|
| Beginner / titration | 50 | 1 day range | SubQ |
| Anti-aging and GH optimization | 100 | 2 day range | SubQ |
| Sleep quality and recovery | 150 | 1 day range | SubQ |
| Fat loss | 200 | 2 day range | SubQ |
| Muscle growth and body recomposition | 250 | 3 day range | SubQ |
| Aggressive GH pulse (stacked with GHRP) | 300 | 3 day range | SubQ |
Reconstitution Instructions
Materials needed:
Steps to reconstitute
- Remove the plastic cap from the CJC-1295 vial and wipe the rubber stopper with a 70% isopropyl alcohol swab — allow it to fully dry before proceeding
- Draw the desired volume of bacteriostatic water (BAC water) into a 1 mL insulin syringe — 2 mL BAC water into a 2 mg vial yields a 1 mg/mL (1000 mcg/mL) working concentration
- Insert the needle through the rubber stopper at a slight angle, directing the tip toward the inner vial wall rather than pointing at the powder
- Depress the plunger slowly so the BAC water flows down the vial wall — do not inject directly onto the powder
- Gently swirl the vial in slow circular motions until the powder is fully dissolved — do not shake or vortex
- Allow the solution to rest undisturbed for 2–3 minutes to ensure complete dissolution before drawing a dose
- Store reconstituted vial in the refrigerator at 2–8°C and use within 30 days — discard if solution appears cloudy or has particulate matter
- To inject: wipe the injection site with an alcohol swab, pinch a fold of skin on the abdomen at least 2 inches from the navel, insert the insulin needle at a 45-degree angle, and slowly depress the plunger
- Withdraw the needle and apply gentle pressure with a clean swab — do not rub the site
- Rotate injection sites each administration, alternating between left abdomen, right abdomen, left thigh, and right thigh
CJC-1295 (without DAC) Cycle
The CJC-1295 (without DAC) Cycle section explains how long a typical cycle lasts and what to expect during each phase. Over time, your body can become less responsive with continuous use.
Taking breaks between cycles may help maintain effectiveness and support better overall results.
- Day 1-7
- Improved sleep quality; vivid dreams from enhanced REM
- Week 2-4
- Enhanced workout recovery, reduced muscle soreness, better pumps
- Week 4-8
- Noticeable skin improvements, minor body composition shifts
- Week 8-12
- Significant lean gains, fat loss, improved joint comfort
- Week 0-0
- Continued gradual improvements in composition and well-being
Dosing tools
CJC-1295 (without DAC) Peptide Dosage Calculator
Calculate peptide doses with our visual syringe guide.
mg
Enter the total amount of peptide in the vial in milligrams (as stated on the label).
The dose you want to inject per administration, in mcg or mg.
1,000 mcg = 1 mg
1 mL
2 mL
3 mL
5 mL
Custom
Volume of bacteriostatic water you add to reconstitute the powder. Use BAC water for preservation.
Injection Results
Based on your vial and dilution inputs.
CJC-1295 (without DAC)
SINGLE COMPOUNDVolume per injection
0.05
mL
Concentration
10.00
mg/mL
Doses per vial
20
doses
Total injections per vial
20 injections
How it works
Based on a 10 mg CJC-1295 (without DAC) vial diluted with 1 mL of bacteriostatic water, each 500 mcg injection equals 0.05 mL.
1mL / 100 units
5 units
0.050 mL
Reference Guide
Dosing Cycle
- Peptide
- CJC-1295 (without DAC)
- Dosing
- 100–300mcg per injection
- Dosing Frequency
- once daily, before sleep or 30 minutes pre-workout
- Cycle Duration
- 8–12 weeks on, 4 weeks off
- Storage
- Lyophilized: -20°C for up to 24–36 months. Reconstituted with bacteriostatic water: 2–8°C for up to 28 days. Do not freeze reconstituted solution.
Note: Triple agonist; microdose for fewer side effects
Reconstitution Tips
- Use bacteriostatic water (BAC) — contains 0.9% benzyl alcohol for preservation
- Inject water slowly — aim down the vial wall, not directly onto powder
- Never shake — gently swirl or roll the vial until dissolved
- Store properly — refrigerate at 2-8°C after reconstitution
- Use within 28 days — most reconstituted peptides remain stable for about 4 weeks
- Keep sterile — always clean vial tops with alcohol before drawing
Peptide Interactions
Research suggestions of CJC-1295 (without DAC) interactions with other common peptides and substances.
Weight
ipa
Growth
Ipamorelin
SYNERGISTIC
Complementary GH release via different receptor pathways. Popular combination.
Weight
mk6
Growth
MK-677
COMPATIBLE
Different mechanisms; continuous ghrelin versus pulsatile GHRH.
Weight
tesa
Growth
Tesamorelin
MONITOR
Both are GHRH analogs; combining offers no additional benefit.
Side effects
Avoid: Do not take CJC-1295 (without DAC) with CJC-1295 with DAC.
Monitor: Be careful when combining CJC-1295 (without DAC) with Hexarelin, Tesamorelin.
Contraindications
Active cancer (due to growth-promoting effects)
Diabetic retinopathy
Severe kidney disease
Pregnancy or breastfeeding
Stop signs
Persistent joint pain or carpal tunnel symptoms
Significant water retention or edema
Unexplained headaches or vision changes
Extreme fatigue or lethargy
Allergic reaction signs at injection sites
Bad signs
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Frequently asked questions
What is a peptide dosage calculator?
A peptide dosage calculator is a free tool that converts your vial size, bacteriostatic water volume, and target dose into an exact syringe draw volume. Instead of doing the reconstitution math by hand, you enter three inputs and instantly get the concentration of your solution and how many milliliters or syringeunits to draw. This calculator works for single peptide compounds and multi-peptide blends.
How do I calculate peptide dosage from a vial?
To calculate your peptide dose, divide the total peptide content of your vial in micrograms by the volume of bacteriostatic water you added in milliliters. This gives you your solution concentration in mcg/mL. Then divide your target dose by that concentration to get your draw volume. For example, a 5mg (5,000 mcg) vial reconstituted with 2mL of BAC water gives a concentration of 2,500 mcg/mL. A 250 mcg dose would require drawing 0.1mL. This calculator automates all of those steps instantly.
How much Bacteriostatic water should I add to a peptide vial?
Most people add 2mL to 3mL of bacteriostatic water per vial, but the right amount depends on the dose you want to draw and the syringe size you are using. Adding 1mL to a 5mg vial gives you a concentration of 5,000 mcg/mL, making each dose very small in volume. Adding 2mL gives you 2,500 mcg/mL, which is easier to measure on a standard insulin syringe. A general guideline is to choose a volume that puts your typical dose somewhere between 10 and 30 units on a U-100 syringe. Use the calculator above to test different water volumes and find what works for your dose.
How are peptides different from proteins?
Both are made of amino acids, but peptides are much smaller than proteins. Because of their tiny size, peptides can act like tiny messengers in the body, sending specific signals to your cells to tell them exactly what to do.
CJC-1295 (without DAC)Research References
CJC-1295 (without DAC) is a phase 2 compound
CJC-1295 (without DAC)
CJC-1295 (without DAC) is a phase 2 compound
Pharmacokinetics of Modified GRF(1-29) in Healthy Adults
Human study with 100-200mcg single dose in 12 subjects showed rapid absorption with peak GH at 15-30 minutes; established half-life and optimal frequency.
2006
Growth Hormone Pulsatility with Mod GRF 1-29
Human study with 100mcg 3x daily for 30 days in 24 subjects preserved natural GH pulsatility patterns with no pituitary desensitization.
2008
Comparative Analysis: CJC-1295 vs Native GHRH
In vitro and human receptor binding assays showed 4x greater receptor affinity than native GHRH with enhanced enzymatic degradation resistance.
2010
Long-term Safety Profile of Modified GRF Analogs
Animal model with 6 months daily administration showed no significant pituitary morphology or function changes supporting chronic safety.
2015
Calculate peptide dosages
Learning how to calculate a peptide dose? Use our beginner-friendly peptide dosage, blend, and accumulation calculators. Enter vial size, reconstitution volume, and target dose to get exact draw volumes instantly. No guesswork, just clear guidance that helps prevent common mistakes.
