PNC-27
FDA APPROVAL PENDING
Emerging
Longevity
PNC-27
0
Amino acids
Molecular weight
Peptide
Type
PNC-27 is an experimental anti-cancer peptide created by a supercomputer at SUNY Downstate Medical Center in 2000. It contains an HDM-2 binding domain from p53 (residues 12-26) linked to a cell-penetrating domain. The peptide selectively kills cancer cells by binding to HDM-2 (MDM2) expressed on cancer cell membranes, forming pores that cause cell necrosis. Critically, PNC-27 has no effect on normal cells because healthy cells don't express HDM-2 on their membranes. Research shows effectiveness against pancreatic cancer, breast cancer, leukemia, and melanoma.
Top researched benefits
Overview of PNC-27
PNC-27 exploits a unique vulnerability of cancer cells: the presence of HDM-2 (human double minute 2, also called MDM2) on their cell surface. The peptide's p53 residues adopt a conformation that binds directly to membrane-bound HDM-2, inducing transmembrane pore formation. This causes rapid tumor cell necrosis (not apoptosis). Additionally, PNC-27 enters cancer cells and disrupts mitochondrial membranes. Normal cells lack surface HDM-2 expression and are completely spared.
cancer research
- PNC-27 shows selective cytotoxicity against pancreatic cancer cells in research.
- Demonstrated effectiveness against breast cancer cell lines.
- Induces necrosis of K-562 leukemia cells through HDM-2 binding.
- Shows selective targeting of melanoma cells.
mechanism studies
- Most effective against cancers with high membrane HDM-2 expression.
- Model compound for studying cancer-selective therapies.
Typical Dose
Not established - preclinical research only
Frequency
Research protocols vary by study
Cycle Duration
Not established - no human clinical trials
Storage
Refrigerate at 2-8°C
No visual available
Due to this peptide having no amino acids, there is no molecular chain to display.
Chemical Makeup
Key benefits
Selectively kills cancer cells only
No effect on normal healthy cells
Induces rapid cancer cell necrosis
Works on multiple cancer types
Unique membrane pore mechanism
Does not require p53 function in target cells
Eradicates tumors in mouse models
Spares human stem cells
Community interest
This peptide is still gaining traction in the community.
Anti-Cancer Peptide | p53-HDM-2 Disruptor
This overview is informational and based on aggregated descriptions from studies and user reports.
Was it helpful?YesNoPNC-27 Protocols
PNC-27 is currently in preclinical research stages. All dosing data comes from in vitro and animal model studies. No human dosing protocols have been approved. The peptide has shown tumor eradication in nude mice xenograft models.
| Goal | Dosage | Frequency | Route |
|---|---|---|---|
| Research protocol | — | — | IP or direct tumor injection (research) |
Reconstitution Instructions
Materials needed:
Steps to reconstitute
- Handle as research chemical
- Reconstitute according to research protocol
- Store reconstituted solution refrigerated
- Use appropriate safety precautions
PNC-27 Cycle
The PNC-27 Cycle section explains how long a typical cycle lasts and what to expect during each phase. Over time, your body can become less responsive with continuous use.
Taking breaks between cycles may help maintain effectiveness and support better overall results.
- Week 0-0
- Binding to membrane HDM-2 begins
- Week 0-0
- Pore formation and cancer cell necrosis
- Week 0-0
- Tumor reduction in animal models
- Week 0-0
- Human clinical trials not yet conducted
Dosing tools
PNC-27 Peptide Dosage Calculator
Calculate peptide doses with our visual syringe guide.
mg
Enter the total amount of peptide in the vial in milligrams (as stated on the label).
The dose you want to inject per administration, in mcg or mg.
1,000 mcg = 1 mg
1 mL
2 mL
3 mL
5 mL
Custom
Volume of bacteriostatic water you add to reconstitute the powder. Use BAC water for preservation.
Injection Results
Based on your vial and dilution inputs.
PNC-27
SINGLE COMPOUNDVolume per injection
0.05
mL
Concentration
10.00
mg/mL
Doses per vial
20
doses
Total injections per vial
20 injections
How it works
Based on a 10 mg PNC-27 vial diluted with 1 mL of bacteriostatic water, each 500 mcg injection equals 0.05 mL.
1mL / 100 units
5 units
0.050 mL
Reference Guide
Dosing Cycle
- Peptide
- PNC-27
- Dosing
- Not established - preclinical research only
- Dosing Frequency
- Research protocols vary by study
- Cycle Duration
- Not established - no human clinical trials
- Storage
- Refrigerate at 2-8°C
Note: Triple agonist; microdose for fewer side effects
Reconstitution Tips
- Use bacteriostatic water (BAC) — contains 0.9% benzyl alcohol for preservation
- Inject water slowly — aim down the vial wall, not directly onto powder
- Never shake — gently swirl or roll the vial until dissolved
- Store properly — refrigerate at 2-8°C after reconstitution
- Use within 28 days — most reconstituted peptides remain stable for about 4 weeks
- Keep sterile — always clean vial tops with alcohol before drawing
Side effects
Contraindications
Not approved for human use
Experimental research peptide only
Cancers without membrane HDM-2 may not respond
Stop signs
Not applicable - not approved for human use
Bad signs
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Frequently asked questions
What is a peptide dosage calculator?
A peptide dosage calculator is a free tool that converts your vial size, bacteriostatic water volume, and target dose into an exact syringe draw volume. Instead of doing the reconstitution math by hand, you enter three inputs and instantly get the concentration of your solution and how many milliliters or syringeunits to draw. This calculator works for single peptide compounds and multi-peptide blends.
How do I calculate peptide dosage from a vial?
To calculate your peptide dose, divide the total peptide content of your vial in micrograms by the volume of bacteriostatic water you added in milliliters. This gives you your solution concentration in mcg/mL. Then divide your target dose by that concentration to get your draw volume. For example, a 5mg (5,000 mcg) vial reconstituted with 2mL of BAC water gives a concentration of 2,500 mcg/mL. A 250 mcg dose would require drawing 0.1mL. This calculator automates all of those steps instantly.
How much Bacteriostatic water should I add to a peptide vial?
Most people add 2mL to 3mL of bacteriostatic water per vial, but the right amount depends on the dose you want to draw and the syringe size you are using. Adding 1mL to a 5mg vial gives you a concentration of 5,000 mcg/mL, making each dose very small in volume. Adding 2mL gives you 2,500 mcg/mL, which is easier to measure on a standard insulin syringe. A general guideline is to choose a volume that puts your typical dose somewhere between 10 and 30 units on a U-100 syringe. Use the calculator above to test different water volumes and find what works for your dose.
How are peptides different from proteins?
Both are made of amino acids, but peptides are much smaller than proteins. Because of their tiny size, peptides can act like tiny messengers in the body, sending specific signals to your cells to tell them exactly what to do.
PNC-27Research References
PNC-27 is an emerging compound
PNC-27
PNC-27 is an emerging compound
Anticancer peptide PNC-27 adopts an HDM-2-binding conformation and kills cancer cells by binding to HDM-2 in their membranes
PNC-27 binds membrane-bound HDM-2, inducing pore formation and cancer cell necrosis while sparing normal cells.
n.d.
Anti-Cancer Peptide PNC-27 Kills Cancer Cells by Unique Interactions with Plasma Membrane-Bound hdm-2 and with Mitochondrial Membranes
PNC-27 also enters cancer cells and disrupts mitochondrial membranes.
n.d.
PNC-27 Induces Tumor Cell Necrosis of Leukemia Cells
PNC-27 induces necrosis of K-562 leukemia cells lacking p53 through p53-independent pathway.
n.d.
PNC-27, a Chimeric p53-Penetratin Peptide
PNC-27 binds HDM-2 in p53 peptide-like structure, induces selective membrane-pore formation leading to cancer cell lysis.
n.d.