Growth
MGF
Healing
24
Amino acids
Molecular weight
Peptide
Type
Non-pegylated IGF-1 splice variant produced locally in muscle tissue following mechanical stress. Has a very short half-life compared to PEG-MGF, requiring more frequent administration or localized injection.
Top researched benefits
Overview of MGF
Activates muscle satellite stem cells via receptor binding, stimulating MAPK/ERK signaling. Enhances protein synthesis and promotes muscle fiber repair. The E-peptide domain exhibits distinct activity from mature IGF-1.
muscle repair
- Primary mechanism activating dormant muscle satellite cells which fuse to damaged fibers.
- Short half-life means effects are concentrated at injection site.
- Naturally upregulated after mechanical stress; supplementation enhances repair processes.
tissue regeneration
- Animal studies suggest improved tendon injury outcomes when applied locally.
- Research indicates potential for bone healing via osteoblast regulation.
Typical Dose
100-300mcg per injection
Frequency
Daily, immediately post-workout
Cycle Duration
8-12 weeks
Storage
Refrigerate at 2-8°C; use within 30 days
Chemical Makeup
Key benefits
Satellite cell activation for muscle repair
Recovery support following mechanical stress
Localized tissue regeneration
Natural upregulation after exercise
Community interest
This peptide is still gaining traction in the community.
Mechano Growth Factor | IGF-1 Splice Variant
This overview is informational and based on aggregated descriptions from studies and user reports.
Was it helpful?YesNoMGF Molecular Information
View the scientifc details of MGF.
24
Amino Acids
MGF
Tyr
Tyr
Position 1
Gln
Gln
Position 2
Pro
Pro
Position 3
Pro
Pro
Position 4
Ser
Ser
Position 5
Thr
Thr
Position 6
Asn
Asn
Position 7
Lys
Lys
Position 8
Asn
Asn
Position 9
Thr
Thr
Position 10
Lys
Lys
Position 11
Ser
Ser
Position 12
Gln
Gln
Position 13
Arg
Arg
Position 14
Arg
Arg
Position 15
Lys
Lys
Position 16
Gly
Gly
Position 17
Ser
Ser
Position 18
Thr
Thr
Position 19
Phe
Phe
Position 20
Glu
Glu
Position 21
Glu
Glu
Position 22
His
His
Position 23
Lys
Lys
Position 24
Molecular Weight
0DaChain Length
24Amino AcidsType
PeptideMGF Protocols
Intramuscular injection directly into target muscle immediately post-workout for localized effects due to short half-life.
| Goal | Dosage | Frequency | Route |
|---|---|---|---|
| Standard Protocol | 100 | 1 week range | IM into target muscle |
| Progressive Titration | 100 | 1 week range | IM bilateral |
| Localized Recovery | 200 | — | IM into worked muscle groups |
Reconstitution Instructions
Materials needed:
Steps to reconstitute
- Allow vial to reach room temperature
- Clean rubber stopper with alcohol swab
- Add 3.0mL bacteriostatic water for ~1.67mg/mL concentration
- Inject slowly along vial wall, not directly onto powder
- Gently swirl to dissolve; never shake
- Store refrigerated at 2-8°C
- Use within 30 days of reconstitution
MGF Cycle
The MGF Cycle section explains how long a typical cycle lasts and what to expect during each phase. Over time, your body can become less responsive with continuous use.
Taking breaks between cycles may help maintain effectiveness and support better overall results.
- Week 0-0
- Very short half-life means localized effects at injection site within minutes
- Week 1-2
- Reduced muscle soreness in targeted areas; subtle recovery improvements
- Week 4-8
- Improved recovery in targeted muscles; enhanced training capacity
Dosing tools
MGF Peptide Dosage Calculator
Calculate peptide doses with our visual syringe guide.
mg
Enter the total amount of peptide in the vial in milligrams (as stated on the label).
The dose you want to inject per administration, in mcg or mg.
1,000 mcg = 1 mg
1 mL
2 mL
3 mL
5 mL
Custom
Volume of bacteriostatic water you add to reconstitute the powder. Use BAC water for preservation.
Injection Results
Based on your vial and dilution inputs.
MGF
SINGLE COMPOUNDVolume per injection
0.05
mL
Concentration
10.00
mg/mL
Doses per vial
20
doses
Total injections per vial
20 injections
How it works
Based on a 10 mg MGF vial diluted with 1 mL of bacteriostatic water, each 500 mcg injection equals 0.05 mL.
1mL / 100 units
5 units
0.050 mL
Reference Guide
Dosing Cycle
- Peptide
- MGF
- Dosing
- 100-300mcg per injection
- Dosing Frequency
- Daily, immediately post-workout
- Cycle Duration
- 8-12 weeks
- Storage
- Refrigerate at 2-8°C; use within 30 days
Note: Triple agonist; microdose for fewer side effects
Reconstitution Tips
- Use bacteriostatic water (BAC) — contains 0.9% benzyl alcohol for preservation
- Inject water slowly — aim down the vial wall, not directly onto powder
- Never shake — gently swirl or roll the vial until dissolved
- Store properly — refrigerate at 2-8°C after reconstitution
- Use within 28 days — most reconstituted peptides remain stable for about 4 weeks
- Keep sterile — always clean vial tops with alcohol before drawing
Peptide Interactions
Research suggestions of MGF interactions with other common peptides and substances.
Healing
bpc
Longevity
BPC-157
SYNERGISTIC
Complementary mechanisms. BPC-157 promotes angiogenesis; MGF activates satellite cells.
Growth
igf
Healing
IGF-1 LR3
MONITOR
Both target IGF-1 pathways; combining risks receptor overstimulation.
Growth
peg
Healing
PEG-MGF
MONITOR
Same active peptide with different half-lives. Combining is redundant; choose one based on protocol preference.
Healing
tb5
Longevity
TB-500
SYNERGISTIC
TB-500 reduces inflammation and promotes cell migration; MGF activates muscle stem cells.
Side effects
Monitor: Be careful when combining MGF with IGF-1 LR3, PEG-MGF.
Contraindications
Any history of cancer or neoplastic disease
Pregnancy or breastfeeding
Uncontrolled diabetes
Stop signs
Any unusual growths, lumps, or rapid tissue changes
Severe injection site reactions
Persistent headaches or vision changes
Signs of hypoglycemia
Bad signs
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Frequently asked questions
What is a peptide dosage calculator?
A peptide dosage calculator is a free tool that converts your vial size, bacteriostatic water volume, and target dose into an exact syringe draw volume. Instead of doing the reconstitution math by hand, you enter three inputs and instantly get the concentration of your solution and how many milliliters or syringeunits to draw. This calculator works for single peptide compounds and multi-peptide blends.
How do I calculate peptide dosage from a vial?
To calculate your peptide dose, divide the total peptide content of your vial in micrograms by the volume of bacteriostatic water you added in milliliters. This gives you your solution concentration in mcg/mL. Then divide your target dose by that concentration to get your draw volume. For example, a 5mg (5,000 mcg) vial reconstituted with 2mL of BAC water gives a concentration of 2,500 mcg/mL. A 250 mcg dose would require drawing 0.1mL. This calculator automates all of those steps instantly.
How much Bacteriostatic water should I add to a peptide vial?
Most people add 2mL to 3mL of bacteriostatic water per vial, but the right amount depends on the dose you want to draw and the syringe size you are using. Adding 1mL to a 5mg vial gives you a concentration of 5,000 mcg/mL, making each dose very small in volume. Adding 2mL gives you 2,500 mcg/mL, which is easier to measure on a standard insulin syringe. A general guideline is to choose a volume that puts your typical dose somewhere between 10 and 30 units on a U-100 syringe. Use the calculator above to test different water volumes and find what works for your dose.
How are peptides different from proteins?
Both are made of amino acids, but peptides are much smaller than proteins. Because of their tiny size, peptides can act like tiny messengers in the body, sending specific signals to your cells to tell them exactly what to do.
MGFResearch References
MGF is a limited compound
MGF
MGF is a limited compound
MGF-E Peptide Human Muscle Cell Study
MGF significantly increased satellite cell proliferation at 3ng/ml with age-dependent effects.
2011
Mechano-Growth Factor Minireview
Overview of MGF as IGF-I gene product involved in tissue repair, upregulated following exercise and injury.
2010