Growth
MGF
Healing
24
Amino acids
Molecular weight
Peptide
Type
MGF (Mechano Growth Factor), also designated IGF-IEc, is a 24-amino acid C-terminal splice variant of IGF-1 produced locally in skeletal muscle in response to mechanical stress or injury. It activates muscle satellite cells, promotes their proliferation and fusion, and initiates site-specific tissue repair distinct from systemic IGF-1 signaling. Used primarily in research and by performance-focused individuals seeking accelerated muscle recovery and hypertrophy.
Top researched benefits
Overview of MGF
MGF acts independently of the IGF-1 receptor, signaling through the MAPK-Erk1/2 pathway and PKC-mediated Nrf2 activation to drive satellite cell proliferation and delay cellular senescence. Upon mechanical overload or tissue damage, locally expressed MGF recruits and expands the muscle satellite cell pool, increasing myonuclear number and enabling new fiber growth rather than simple fiber hypertrophy.
muscle repair
- Primary mechanism activating dormant muscle satellite cells which fuse to damaged fibers.
- Short half-life means effects are concentrated at injection site.
- Naturally upregulated after mechanical stress; supplementation enhances repair processes.
tissue regeneration
- Animal studies suggest improved tendon injury outcomes when applied locally.
- Research indicates potential for bone healing via osteoblast regulation.
Typical Dose
200mcg per injection
Frequency
2–3 times per week post-workout
Cycle Duration
4–6 week cycles
Storage
Lyophilized: store at -20°C or below for up to 1 year; stable at room temperature for 3–4 weeks. Reconstituted: refrigerate at 2–8°C and use within 10–14 days; avoid repeated freeze-thaw cycles.
Chemical Makeup
Key benefits
Activates quiescent muscle satellite cells post-exercise to replenish the myonuclear pool, enabling genuine new muscle fiber formation rather than enlargement of existing fibers
Accelerates recovery from skeletal muscle microtrauma by providing a localized, site-specific repair signal at the injury site
Delays satellite cell senescence and extends proliferative lifespan, offering a potential strategy to counter age-related sarcopenia
Promotes chondrocyte proliferation, migration, and differentiation, supporting cartilage repair and protection in joint injury models
Community interest
This peptide is still gaining traction in the community.
IGF-1 Splice Variant | Muscle Repair & Satellite Cell Activation
This overview is informational and based on aggregated descriptions from studies and user reports.
Was it helpful?YesNoMGF Molecular Information
View the scientifc details of MGF.
24
Amino Acids
MGF
Tyr
Tyr
Position 1
Gln
Gln
Position 2
Pro
Pro
Position 3
Pro
Pro
Position 4
Ser
Ser
Position 5
Thr
Thr
Position 6
Asn
Asn
Position 7
Lys
Lys
Position 8
Asn
Asn
Position 9
Thr
Thr
Position 10
Lys
Lys
Position 11
Ser
Ser
Position 12
Gln
Gln
Position 13
Arg
Arg
Position 14
Arg
Arg
Position 15
Lys
Lys
Position 16
Gly
Gly
Position 17
Ser
Ser
Position 18
Thr
Thr
Position 19
Phe
Phe
Position 20
Glu
Glu
Position 21
Glu
Glu
Position 22
His
His
Position 23
Lys
Lys
Position 24
Molecular Weight
2868.17DaChain Length
24Amino AcidsType
PeptideMGF Protocols
Subcutaneous or intramuscular injection is the primary delivery route for MGF, with IM injection directly into the trained or injured muscle group preferred for localized satellite cell activation. Due to MGF's extremely short half-life of 5–7 minutes, timing within 30–60 minutes post-workout is critical for maximum effect.
| Goal | Dosage | Frequency | Route |
|---|---|---|---|
| Muscle hypertrophy and satellite cell activation | 200 | 1 day range | IM into trained muscle group |
| Advanced muscle growth | 400 | 1 day range | IM into trained muscle group |
| Muscle injury repair | 200 | 3 week range | SubQ or IM near injury site |
| Recovery and post-workout repair | 100 | 5 week range | IM bilateral administration |
| Age-related muscle preservation | 200 | 3 week range | SubQ or IM |
| Cardiac muscle research protocol | 200 | 3 week range | SubQ |
Reconstitution Instructions
Materials needed:
Steps to reconstitute
- Allow the MGF vial to reach room temperature before handling
- Draw 2 mL of bacteriostatic water into an insulin syringe
- Inject the bacteriostatic water slowly down the inside wall of the vial — never directly onto the powder
- Gently rotate the vial between your palms until the powder fully dissolves into a clear solution — do not shake
- Allow the reconstituted vial to rest for 5–10 minutes if any particles remain
- Draw the desired dose into a fresh insulin syringe, removing any air bubbles
- For IM injection, insert the needle at a 90-degree angle into the target muscle group (e.g., quads, delts, biceps after training that muscle); for SubQ, pinch skin and inject at 45 degrees into belly fat or near the injury site
- Inject slowly and steadily, then withdraw the needle and apply light pressure — do not massage the site
- Store reconstituted vial refrigerated at 2–8°C and use within 30 days
MGF Cycle
The MGF Cycle section explains how long a typical cycle lasts and what to expect during each phase. Over time, your body can become less responsive with continuous use.
Taking breaks between cycles may help maintain effectiveness and support better overall results.
- Week 0-0
- Very short half-life means localized effects at injection site within minutes
- Week 1-2
- Reduced muscle soreness in targeted areas; subtle recovery improvements
- Week 4-8
- Improved recovery in targeted muscles; enhanced training capacity
Dosing tools
MGF Peptide Dosage Calculator
Calculate peptide doses with our visual syringe guide.
mg
Enter the total amount of peptide in the vial in milligrams (as stated on the label).
The dose you want to inject per administration, in mcg or mg.
1,000 mcg = 1 mg
1 mL
2 mL
3 mL
5 mL
Custom
Volume of bacteriostatic water you add to reconstitute the powder. Use BAC water for preservation.
Injection Results
Based on your vial and dilution inputs.
MGF
SINGLE COMPOUNDVolume per injection
0.05
mL
Concentration
10.00
mg/mL
Doses per vial
20
doses
Total injections per vial
20 injections
How it works
Based on a 10 mg MGF vial diluted with 1 mL of bacteriostatic water, each 500 mcg injection equals 0.05 mL.
1mL / 100 units
5 units
0.050 mL
Reference Guide
Dosing Cycle
- Peptide
- MGF
- Dosing
- 200mcg per injection
- Dosing Frequency
- 2–3 times per week post-workout
- Cycle Duration
- 4–6 week cycles
- Storage
- Lyophilized: store at -20°C or below for up to 1 year; stable at room temperature for 3–4 weeks. Reconstituted: refrigerate at 2–8°C and use within 10–14 days; avoid repeated freeze-thaw cycles.
Note: Triple agonist; microdose for fewer side effects
Reconstitution Tips
- Use bacteriostatic water (BAC) — contains 0.9% benzyl alcohol for preservation
- Inject water slowly — aim down the vial wall, not directly onto powder
- Never shake — gently swirl or roll the vial until dissolved
- Store properly — refrigerate at 2-8°C after reconstitution
- Use within 28 days — most reconstituted peptides remain stable for about 4 weeks
- Keep sterile — always clean vial tops with alcohol before drawing
Peptide Interactions
Research suggestions of MGF interactions with other common peptides and substances.
Healing
bpc
Longevity
BPC-157
SYNERGISTIC
Complementary mechanisms. BPC-157 promotes angiogenesis; MGF activates satellite cells.
Growth
igf
Healing
IGF-1 LR3
MONITOR
Both target IGF-1 pathways; combining risks receptor overstimulation.
Growth
peg
Healing
PEG-MGF
MONITOR
Same active peptide with different half-lives. Combining is redundant; choose one based on protocol preference.
Healing
tb5
Longevity
TB-500
SYNERGISTIC
TB-500 reduces inflammation and promotes cell migration; MGF activates muscle stem cells.
Side effects
Monitor: Be careful when combining MGF with IGF-1 LR3, PEG-MGF.
Contraindications
Any history of cancer or neoplastic disease
Pregnancy or breastfeeding
Uncontrolled diabetes
Stop signs
Any unusual growths, lumps, or rapid tissue changes
Severe injection site reactions
Persistent headaches or vision changes
Signs of hypoglycemia
Bad signs
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Frequently asked questions
What is a peptide dosage calculator?
A peptide dosage calculator is a free tool that converts your vial size, bacteriostatic water volume, and target dose into an exact syringe draw volume. Instead of doing the reconstitution math by hand, you enter three inputs and instantly get the concentration of your solution and how many milliliters or syringeunits to draw. This calculator works for single peptide compounds and multi-peptide blends.
How do I calculate peptide dosage from a vial?
To calculate your peptide dose, divide the total peptide content of your vial in micrograms by the volume of bacteriostatic water you added in milliliters. This gives you your solution concentration in mcg/mL. Then divide your target dose by that concentration to get your draw volume. For example, a 5mg (5,000 mcg) vial reconstituted with 2mL of BAC water gives a concentration of 2,500 mcg/mL. A 250 mcg dose would require drawing 0.1mL. This calculator automates all of those steps instantly.
How much Bacteriostatic water should I add to a peptide vial?
Most people add 2mL to 3mL of bacteriostatic water per vial, but the right amount depends on the dose you want to draw and the syringe size you are using. Adding 1mL to a 5mg vial gives you a concentration of 5,000 mcg/mL, making each dose very small in volume. Adding 2mL gives you 2,500 mcg/mL, which is easier to measure on a standard insulin syringe. A general guideline is to choose a volume that puts your typical dose somewhere between 10 and 30 units on a U-100 syringe. Use the calculator above to test different water volumes and find what works for your dose.
How are peptides different from proteins?
Both are made of amino acids, but peptides are much smaller than proteins. Because of their tiny size, peptides can act like tiny messengers in the body, sending specific signals to your cells to tell them exactly what to do.
MGFResearch References
MGF is a preclinical compound
MGF
MGF is a preclinical compound
MGF-E Peptide Human Muscle Cell Study
MGF significantly increased satellite cell proliferation at 3ng/ml with age-dependent effects.
2011
Mechano-Growth Factor Minireview
Overview of MGF as IGF-I gene product involved in tissue repair, upregulated following exercise and injury.
2010
Calculate peptide dosages
Learning how to calculate a peptide dose? Use our beginner-friendly peptide dosage, blend, and accumulation calculators. Enter vial size, reconstitution volume, and target dose to get exact draw volumes instantly. No guesswork, just clear guidance that helps prevent common mistakes.
