TB-500 (Ac-LKKTETQ)

FDA APPROVAL PENDING

Moderate Research

Healing

Longevity

TB-500 (Ac-LKKTETQ)

Thymosin Beta-4 Active Fragment | Healing Peptide

Learn more

Healing

TB-500 (Ac-LKKTETQ)

Longevity

Amino acid sequence

7

Amino acids

889da

Molecular weight

Peptide

Type

TB-500 (Ac-LKKTETQ) is a synthetic derivative of thymosin beta-4 consisting of the N-terminal acetylated 17-23 amino acid fragment. This sequence represents the active site within thymosin beta-4 responsible for actin binding, cell migration, and wound healing. Research shows it promotes endothelial cell differentiation, angiogenesis, keratinocyte migration, collagen deposition, and decreases inflammation. The acetylation protects against N-terminal degradation while maintaining biological activity.

Top researched benefits

Overview of TB-500 (Ac-LKKTETQ)

TB-500 contains the LKKTETQ sequence which is the actin-binding motif of full-length thymosin beta-4. This fragment shares many properties of the parent protein regarding cell proliferation, differentiation, and migration. It promotes angiogenesis by upregulating VEGF expression and enhancing endothelial cell sprouting. The peptide undergoes serial cleavage at the C-terminus during metabolism, while the N-terminal acetylation provides protection from degradation.

tissue repair

  • Promotes dermal wound healing through the active LKKTETQ sequence.
  • Supports tissue repair comparable to full thymosin beta-4 in research.
  • Promotes keratinocyte migration and collagen deposition.

vascular support

  • Promotes endothelial cell differentiation and new blood vessel formation.

antiinflammatory

  • Decreases inflammatory responses in damaged tissues.

Typical Dose

2-2.5mg per injection

Frequency

2x weekly (e.g., Monday and Thursday)

Cycle Duration

4-6 weeks loading phase

Storage

Reconstituted: 2-8°C refrigerated

Chemical Makeup

Key benefits

Promotes wound healing

Enhances cell migration

Supports angiogenesis (new blood vessel formation)

Reduces inflammation

Promotes keratinocyte migration

Supports collagen deposition

Enhances tissue repair

Active region of thymosin beta-4

Community interest

This peptide is still gaining traction in the community.

Thymosin Beta-4 Active Fragment | Healing Peptide

This overview is informational and based on aggregated descriptions from studies and user reports.

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TB-500 (Ac-LKKTETQ) Molecular Information

View the scientifc details of TB-500 (Ac-LKKTETQ).

7

Amino Acids

TB-500 (Ac-LKKTETQ)

Leu

Leu

Position 1

Lys

Lys

Position 2

Lys

Lys

Position 3

Thr

Thr

Position 4

Glu

Glu

Position 5

Thr

Thr

Position 6

Gln

Gln

Position 7

Amino acid sequence
LeucinePosition 1
LysinePosition 2
LysinePosition 3
ThreoninePosition 4
Glutamic acidPosition 5
ThreoninePosition 6
GlutaminePosition 7

Molecular Weight

889Da

Chain Length

7Amino Acids

Type

Peptide

TB-500 (Ac-LKKTETQ) Protocols

TB-500 is typically administered via subcutaneous injection. The N-terminal acetylation provides stability and protection from degradation. Not authorized for medicinal use; research peptide only.

GoalDosageFrequencyRoute
Loading phase22 week rangeSubQ
Maintenance2—SubQ

Reconstitution Instructions

Materials needed:

Bacteriostatic waterInsulin syringesAlcohol swabsPeptide vialSterile work surface

Steps to reconstitute

  1. Clean work area and hands thoroughly
  2. Calculate required BAC water volume
  3. Draw BAC water into syringe
  4. Inject slowly down vial side
  5. Gently swirl until dissolved (never shake)
  6. Store reconstituted solution refrigerated

TB-500 (Ac-LKKTETQ) Cycle

The TB-500 (Ac-LKKTETQ) Cycle section explains how long a typical cycle lasts and what to expect during each phase. Over time, your body can become less responsive with continuous use.

Taking breaks between cycles may help maintain effectiveness and support better overall results.

Day 1-7
Initial wound healing acceleration
Week 1-2
Enhanced cell migration and angiogenesis
Week 2-4
Visible tissue repair improvements
Week 4-6
Full healing benefits; transition to maintenance

Dosing tools

TB-500 (Ac-LKKTETQ) Peptide Dosage Calculator

Calculate peptide doses with our visual syringe guide.

mg

Enter the total amount of peptide in the vial in milligrams (as stated on the label).

The dose you want to inject per administration, in mcg or mg.

1,000 mcg = 1 mg

1 mL

2 mL

3 mL

5 mL

Custom

Volume of bacteriostatic water you add to reconstitute the powder. Use BAC water for preservation.

Injection Results

Based on your vial and dilution inputs.

TB-500 (Ac-LKKTETQ)

SINGLE COMPOUND

Volume per injection

0.05

mL

Concentration

10.00

mg/mL

Doses per vial

20

doses

Total injections per vial

20 injections

How it works

Based on a 10 mg TB-500 (Ac-LKKTETQ) vial diluted with 1 mL of bacteriostatic water, each 500 mcg injection equals 0.05 mL.

1mL / 100 units

5 units

0.050 mL

Reference Guide

Dosing Cycle

Peptide
TB-500 (Ac-LKKTETQ)
Dosing
2-2.5mg per injection
Dosing Frequency
2x weekly (e.g., Monday and Thursday)
Cycle Duration
4-6 weeks loading phase
Storage
Reconstituted: 2-8°C refrigerated

Note: Triple agonist; microdose for fewer side effects

Reconstitution Tips

  • Use bacteriostatic water (BAC) — contains 0.9% benzyl alcohol for preservation
  • Inject water slowly — aim down the vial wall, not directly onto powder
  • Never shake — gently swirl or roll the vial until dissolved
  • Store properly — refrigerate at 2-8°C after reconstitution
  • Use within 28 days — most reconstituted peptides remain stable for about 4 weeks
  • Keep sterile — always clean vial tops with alcohol before drawing

Peptide Interactions

Research suggestions of TB-500 (Ac-LKKTETQ) interactions with other common peptides and substances.

Healing

bpc

Longevity

BPC-157

SYNERGISTIC

Commonly combined for enhanced healing effects through different mechanisms.

SYNERGISTIC

Healing

ghk

Longevity

GHK-Cu

SYNERGISTIC

Both support wound healing and tissue repair through different pathways.

SYNERGISTIC

Growth

igf

Healing

IGF-1 LR3

COMPATIBLE

Different mechanisms; may complement for tissue growth.

COMPATIBLE

Healing

thymos

Longevity

Thymosin Beta-4

COMPATIBLE

TB-500 is the active fragment; combining may be redundant.

COMPATIBLE

Side effects

Contraindications

Not authorized for medicinal use

Active cancer (theoretical concern)

Pregnancy or breastfeeding

Prohibited by WADA in sports

Stop signs

Allergic reactions

Unusual swelling or inflammation

Bad signs

DiscolorationCloudy solutionParticulates visible
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Frequently asked questions

What is a peptide dosage calculator?

A peptide dosage calculator is a free tool that converts your vial size, bacteriostatic water volume, and target dose into an exact syringe draw volume. Instead of doing the reconstitution math by hand, you enter three inputs and instantly get the concentration of your solution and how many milliliters or syringeunits to draw. This calculator works for single peptide compounds and multi-peptide blends.

How do I calculate peptide dosage from a vial?

To calculate your peptide dose, divide the total peptide content of your vial in micrograms by the volume of bacteriostatic water you added in milliliters. This gives you your solution concentration in mcg/mL. Then divide your target dose by that concentration to get your draw volume. For example, a 5mg (5,000 mcg) vial reconstituted with 2mL of BAC water gives a concentration of 2,500 mcg/mL. A 250 mcg dose would require drawing 0.1mL. This calculator automates all of those steps instantly.

How much Bacteriostatic water should I add to a peptide vial?

Most people add 2mL to 3mL of bacteriostatic water per vial, but the right amount depends on the dose you want to draw and the syringe size you are using. Adding 1mL to a 5mg vial gives you a concentration of 5,000 mcg/mL, making each dose very small in volume. Adding 2mL gives you 2,500 mcg/mL, which is easier to measure on a standard insulin syringe. A general guideline is to choose a volume that puts your typical dose somewhere between 10 and 30 units on a U-100 syringe. Use the calculator above to test different water volumes and find what works for your dose.

How are peptides different from proteins?

Both are made of amino acids, but peptides are much smaller than proteins. Because of their tiny size, peptides can act like tiny messengers in the body, sending specific signals to your cells to tell them exactly what to do.​

TB-500 (Ac-LKKTETQ)Research References

TB-500 (Ac-LKKTETQ) is a moderate research compound

4Research references

TB-500 (Ac-LKKTETQ)

TB-500 (Ac-LKKTETQ) is a moderate research compound

Synthesis and Characterization of N-terminal Acetylated 17-23 Fragment of Thymosin Beta-4

TB-500 contains Ac-LKKTETQ, the active site responsible for actin binding and wound healing.

n.d.

Doping Control Analysis of TB-500

TB-500 is a synthetic version of the active region of thymosin beta-4.

n.d.

TB-500 Metabolism and Wound Healing

TB-500 undergoes serial cleavage at C-terminus; acetylation protects N-terminus.

n.d.

Investigation of TB-500 Metabolism

Characterized human metabolism of TB-500 using liver microsomes and S9 fraction.

n.d.