Healing
TB-500 (Ac-LKKTETQ)
Longevity
7
Amino acids
Molecular weight
Peptide
Type
TB-500 (Ac-LKKTETQ) is a synthetic heptapeptide corresponding to amino acids 17–23 of thymosin beta-4, a naturally occurring actin-binding protein. It promotes wound healing, tissue repair, and angiogenesis by sequestering G-actin and modulating cytoskeletal reorganization. Used primarily in research and by athletes seeking accelerated recovery from musculoskeletal injuries.
Top researched benefits
Overview of TB-500 (Ac-LKKTETQ)
TB-500 binds G-actin (globular actin) via the LKKTET motif, sequestering monomers to regulate actin polymerization, enabling rapid cytoskeletal reorganization, cell migration, and tissue remodeling. It also upregulates anti-inflammatory pathways and promotes angiogenesis by increasing endothelial cell motility through the same actin-modulating mechanism.
tissue repair
- Promotes dermal wound healing through the active LKKTETQ sequence.
- Supports tissue repair comparable to full thymosin beta-4 in research.
- Promotes keratinocyte migration and collagen deposition.
vascular support
- Promotes endothelial cell differentiation and new blood vessel formation.
antiinflammatory
- Decreases inflammatory responses in damaged tissues.
Typical Dose
2mg per injection
Frequency
twice weekly (loading phase), once weekly (maintenance)
Cycle Duration
8–12 weeks on, 4–8 weeks off
Storage
Lyophilized: store at -20°C long-term or 2–8°C short-term, protected from light and moisture. Reconstituted: refrigerate at 2–8°C, use within 28 days; do not freeze reconstituted solution.
Chemical Makeup
Key benefits
Accelerates musculoskeletal healing by sequestering G-actin and enabling rapid cytoskeletal reorganization at injury sites
Promotes angiogenesis at wound and injury sites by increasing endothelial cell migration and new capillary formation
Reduces systemic and local inflammation by modulating actin-dependent inflammatory cell signaling pathways
Supports tendon and ligament repair with improved tensile strength demonstrated in rat Achilles tendon transection models
Community interest
This peptide is still gaining traction in the community.
Actin-Sequestering Fragment | Tissue Repair & Recovery
This overview is informational and based on aggregated descriptions from studies and user reports.
Was it helpful?YesNoTB-500 (Ac-LKKTETQ) Molecular Information
View the scientifc details of TB-500 (Ac-LKKTETQ).
7
Amino Acids
TB-500 (Ac-LKKTETQ)
Leu
Leu
Position 1
Lys
Lys
Position 2
Lys
Lys
Position 3
Thr
Thr
Position 4
Glu
Glu
Position 5
Thr
Thr
Position 6
Gln
Gln
Position 7
Molecular Weight
846.97DaChain Length
7Amino AcidsType
PeptideTB-500 (Ac-LKKTETQ) Protocols
Subcutaneous injection is the gold-standard delivery route for TB-500 (Ac-LKKTETQ), providing reliable systemic distribution regardless of injection site. The abdominal fat pad or thigh are preferred sites; intramuscular injection near the affected tissue is also used in musculoskeletal recovery protocols.
| Goal | Dosage | Frequency | Route |
|---|---|---|---|
| Tendon and ligament injury repair — initial loading phase to rapidly saturate tissue | 2.5 | 2 week range | SubQ or IM near injury |
| Tendon and ligament injury — sustained repair signaling after loading phase | 2 | 1 week range | SubQ or IM near injury |
| Muscle strain and tear recovery — loading phase to accelerate tissue rebuilding | 2.5 | 2 week range | SubQ or IM near affected muscle |
| Muscle strain and tear recovery — maintenance phase to consolidate healing | 2 | 1 week range | SubQ |
| Chronic joint and inflammation relief — loading to reduce persistent inflammatory signaling | 2 | 2 week range | SubQ near affected joint |
| Post-surgical recovery — intensive loading to support wound healing and tissue remodeling | 2.5 | 2 week range | SubQ |
| Hair growth support — ongoing systemic thymosin beta-4 activity for follicle-related endpoints | 2 | 2 week range | SubQ |
Reconstitution Instructions
Materials needed:
Steps to reconstitute
- Allow the lyophilized TB-500 vial to reach room temperature before opening to prevent condensation inside the vial
- Swab the rubber stopper of the peptide vial with an alcohol wipe and allow it to air dry for 10 seconds
- Draw 2 mL of bacteriostatic water into a sterile syringe for a 5 mg vial (yields 2.5 mg/mL concentration)
- Insert the needle through the rubber stopper at an angle and inject the bacteriostatic water slowly down the inside glass wall — never spray directly onto the powder
- Remove the syringe and gently roll the vial between your palms for 1–2 minutes until fully dissolved — never shake or vortex
- Inspect the solution — it should be clear and colorless to very pale yellow with no visible particles; discard if cloudy or discolored
- Label the reconstituted vial with date and concentration, then refrigerate at 2–8°C; use within 28 days
- Clean the injection site with an alcohol wipe and allow to dry completely
- Pinch a skinfold at the abdomen or thigh, insert the insulin syringe at a 45-degree angle into subcutaneous tissue, and inject slowly and steadily
- Rotate injection sites with each dose to reduce local irritation
TB-500 (Ac-LKKTETQ) Cycle
The TB-500 (Ac-LKKTETQ) Cycle section explains how long a typical cycle lasts and what to expect during each phase. Over time, your body can become less responsive with continuous use.
Taking breaks between cycles may help maintain effectiveness and support better overall results.
- Day 1-7
- Initial wound healing acceleration
- Week 1-2
- Enhanced cell migration and angiogenesis
- Week 2-4
- Visible tissue repair improvements
- Week 4-6
- Full healing benefits; transition to maintenance
Dosing tools
TB-500 (Ac-LKKTETQ) Peptide Dosage Calculator
Calculate peptide doses with our visual syringe guide.
mg
Enter the total amount of peptide in the vial in milligrams (as stated on the label).
The dose you want to inject per administration, in mcg or mg.
1,000 mcg = 1 mg
1 mL
2 mL
3 mL
5 mL
Custom
Volume of bacteriostatic water you add to reconstitute the powder. Use BAC water for preservation.
Injection Results
Based on your vial and dilution inputs.
TB-500 (Ac-LKKTETQ)
SINGLE COMPOUNDVolume per injection
0.05
mL
Concentration
10.00
mg/mL
Doses per vial
20
doses
Total injections per vial
20 injections
How it works
Based on a 10 mg TB-500 (Ac-LKKTETQ) vial diluted with 1 mL of bacteriostatic water, each 500 mcg injection equals 0.05 mL.
1mL / 100 units
5 units
0.050 mL
Reference Guide
Dosing Cycle
- Peptide
- TB-500 (Ac-LKKTETQ)
- Dosing
- 2mg per injection
- Dosing Frequency
- twice weekly (loading phase), once weekly (maintenance)
- Cycle Duration
- 8–12 weeks on, 4–8 weeks off
- Storage
- Lyophilized: store at -20°C long-term or 2–8°C short-term, protected from light and moisture. Reconstituted: refrigerate at 2–8°C, use within 28 days; do not freeze reconstituted solution.
Note: Triple agonist; microdose for fewer side effects
Reconstitution Tips
- Use bacteriostatic water (BAC) — contains 0.9% benzyl alcohol for preservation
- Inject water slowly — aim down the vial wall, not directly onto powder
- Never shake — gently swirl or roll the vial until dissolved
- Store properly — refrigerate at 2-8°C after reconstitution
- Use within 28 days — most reconstituted peptides remain stable for about 4 weeks
- Keep sterile — always clean vial tops with alcohol before drawing
Peptide Interactions
Research suggestions of TB-500 (Ac-LKKTETQ) interactions with other common peptides and substances.
Healing
bpc
Longevity
BPC-157
SYNERGISTIC
Commonly combined for enhanced healing effects through different mechanisms.
Healing
ghk
Longevity
GHK-Cu
SYNERGISTIC
Both support wound healing and tissue repair through different pathways.
Growth
igf
Healing
IGF-1 LR3
COMPATIBLE
Different mechanisms; may complement for tissue growth.
Healing
thymos
Longevity
Thymosin Beta-4
COMPATIBLE
TB-500 is the active fragment; combining may be redundant.
Side effects
Contraindications
Not authorized for medicinal use
Active cancer (theoretical concern)
Pregnancy or breastfeeding
Prohibited by WADA in sports
Stop signs
Allergic reactions
Unusual swelling or inflammation
Bad signs
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Frequently asked questions
What is a peptide dosage calculator?
A peptide dosage calculator is a free tool that converts your vial size, bacteriostatic water volume, and target dose into an exact syringe draw volume. Instead of doing the reconstitution math by hand, you enter three inputs and instantly get the concentration of your solution and how many milliliters or syringeunits to draw. This calculator works for single peptide compounds and multi-peptide blends.
How do I calculate peptide dosage from a vial?
To calculate your peptide dose, divide the total peptide content of your vial in micrograms by the volume of bacteriostatic water you added in milliliters. This gives you your solution concentration in mcg/mL. Then divide your target dose by that concentration to get your draw volume. For example, a 5mg (5,000 mcg) vial reconstituted with 2mL of BAC water gives a concentration of 2,500 mcg/mL. A 250 mcg dose would require drawing 0.1mL. This calculator automates all of those steps instantly.
How much Bacteriostatic water should I add to a peptide vial?
Most people add 2mL to 3mL of bacteriostatic water per vial, but the right amount depends on the dose you want to draw and the syringe size you are using. Adding 1mL to a 5mg vial gives you a concentration of 5,000 mcg/mL, making each dose very small in volume. Adding 2mL gives you 2,500 mcg/mL, which is easier to measure on a standard insulin syringe. A general guideline is to choose a volume that puts your typical dose somewhere between 10 and 30 units on a U-100 syringe. Use the calculator above to test different water volumes and find what works for your dose.
How are peptides different from proteins?
Both are made of amino acids, but peptides are much smaller than proteins. Because of their tiny size, peptides can act like tiny messengers in the body, sending specific signals to your cells to tell them exactly what to do.
TB-500 (Ac-LKKTETQ)Research References
TB-500 (Ac-LKKTETQ) is a preclinical compound
TB-500 (Ac-LKKTETQ)
TB-500 (Ac-LKKTETQ) is a preclinical compound
Synthesis and Characterization of N-terminal Acetylated 17-23 Fragment of Thymosin Beta-4
TB-500 contains Ac-LKKTETQ, the active site responsible for actin binding and wound healing.
n.d.
Doping Control Analysis of TB-500
TB-500 is a synthetic version of the active region of thymosin beta-4.
n.d.
TB-500 Metabolism and Wound Healing
TB-500 undergoes serial cleavage at C-terminus; acetylation protects N-terminus.
n.d.
Investigation of TB-500 Metabolism
Characterized human metabolism of TB-500 using liver microsomes and S9 fraction.
n.d.
Calculate peptide dosages
Learning how to calculate a peptide dose? Use our beginner-friendly peptide dosage, blend, and accumulation calculators. Enter vial size, reconstitution volume, and target dose to get exact draw volumes instantly. No guesswork, just clear guidance that helps prevent common mistakes.
