Reviewed by the PeptideMind Team · Updated July 5, 2026
Free Peptide Dosage Calculator
Enter vial size, bacteriostatic water volume, and target research quantity to calculate the exact draw volume in milliliters. Pre-loaded with half-life and reference dosage data for 100+ research peptides including BPC-157, TB-500, Semaglutide, and Retatrutide. For research purposes only — no signup required.
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Peptide Dosage Calculator: Reconstitution Guide
Get the right peptide dosage every time by mixing the correct amount of bacteriostatic water into your lyophilized vial to produce a properly reconstituted, research-ready solution.
Use bacteriostatic water (BAC)
Contains 0.9% benzyl alcohol for preservation in research solutions.
Add liquid slowly during reconstitution
Aim down the vial wall, not directly onto lyophilized powder.
Never shake
Gently swirl or roll the vial until fully dissolved.
Peptide Dosage: Storage & Handling Guide
Protect your peptide dosage after reconstitution. Proper storage and handling keep your solution stable and free from contamination between uses.
Store properly
Refrigerate reconstituted solutions at 2–8°C.
Use within 28 days
Most reconstituted peptides remain stable for approximately 4 weeks.
Maintain sterility
Clean vial septa with alcohol swabs before each draw.
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How to calculate peptide dosage
Every peptide research quantity calculation follows the same three-variable formula: vial size in mg, reconstitution volume in mL, and target quantity in mcg or mg. For research purposes only.
Enter your vial size
Find the total peptide amount on the vial label and enter it in milligrams (e.g., 10 mg). This is the full quantity of peptide dissolved in your reconstituted solution. Common sizes are 5 mg and 10 mg.
Set your BAC water volume
Select or enter how much bacteriostatic water was used in milliliters — choose from 1, 2, 3, or 5 mL presets, or enter a custom amount. More water means a lower concentration and a larger draw volume for the same research quantity. 2 mL or 3 mL is commonly used in research reconstitution.
Enter the target research quantity
Type in the target research quantity per administration — in mcg or mg, use the unit toggle to switch. The calculator divides the target quantity by the total vial content, then multiplies by the water volume to give the exact draw volume in milliliters.
How to Read Your Syringe
Once the calculator gives you a draw volume in milliliters, you need to locate that measurement on your syringe. Different syringe types use different scales — knowing which one you have prevents dosing errors.
Insulin syringe (U-100)
The most common syringe for peptides. Holds 1 mL, and each mark is 0.01 mL. A dose of 0.25 mL means draw to the 25-unit line.
½ mL insulin syringe (U-50)
Holds 0.5 mL. The marks are more spread out, making small doses easier to measure precisely.
1 mL luer lock syringe
Holds 1 mL, marked directly in mL (no unit conversion). Good for larger doses or a detachable needle.
2 mL luer lock syringe
For doses over 1 mL. Marks are less fine (0.1 mL), so it's a bit less precise for small doses.
Frequently Asked Peptide Calculator Questions
Straight answers on reconstitution, draw volumes, and BAC water ratios, everything you need to use the calculator with confidence. For research reference only.
Bacteriostatic Water vs. Sterile Water
Not all reconstitution water is the same. Knowing the difference between bacteriostatic and sterile water helps you choose the right one for your research protocol.
Bacteriostatic water = sterile water + 0.9% benzyl alcohol
The added benzyl alcohol acts as a preservative, so a single reconstituted vial can be drawn from repeatedly instead of needing to be used all at once.
Sterile water has no preservative
Plain sterile water contains no antimicrobial agent, so once a vial is punctured, any unused solution should be discarded rather than stored for later draws.
Know the contraindications before choosing
Benzyl alcohol carries known contraindications for certain sensitive research contexts, so confirm which water type a given protocol specifies before reconstituting.
Search Peptide Dosage Guides
Search 100+ research peptides for vial size, BAC water, dosing, frequency, and cycle length for 100+ research peptides, including BPC-157, TB-500, Semaglutide, and Retatrutide. Tap any guide to load it straight into the calculator.
Healing
bpc
Longevity
250–500 mcg Daily As needed
10 mg
3 ml
Growth
ser
Growth
500 mcg 2–3×/week 8 weeks
5 mg
3 ml
Weight
GLP-3
Metabolic
0.5–3.0 mg Every other day 8–16 weeks
10 mg
3 ml
Weight
GLP-1
Metabolic
0.25–2.5 mg Weekly 16+ weeks
18 mg
3 ml
Weight
GLP-2
Metabolic
500 mcg–15 mg Weekly 8–16 weeks
10 mg
3 ml
Skin
ghk
Longevity
2 mg 5 days on / 2 days off 6–8 weeks
50 mg
3–4 ml
Growth
ipa
Growth
300 mcg 5 on / 2 off 8–12 weeks
10 mg
3 ml
Cognitive
cer
Longevity
20–32 mg Daily 10–20 consecutive days, repeated 2–4x per year
60 mg
3 ml
Healing
tb50
Longevity
1–2 mg Every 2 days 4–6 weeks
10 mg
3 ml
Metabolic
mot
Metabolic
500 mcg–5 mg 2×/week 4 weeks
10 mg
3 ml
Sexual
PT-141
500–1000 mcg As needed As needed
10 mg
3 ml
Healing
tb5
Longevity
1–2 mg Every 2 days 4–6 weeks
10 mg
3 ml
Free Downloadable Peptide Dosage Cheat Sheets
Reference dosage data for 100+ peptides is pre-loaded in these free cheat sheets, including common research stacks like the wolverine stack (BPC-157 + TB-500), MOTS-C, Retatrutide, and Semaglutide. Select a cheat sheet to see reference dose ranges, dosing frequency, and half-life for every peptide it covers. For research purposes only.
Peptide dosage calculator stats
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Pre-loaded half-life and dosage data for over 100 research peptides.
Instant
draw volume results
Enter vial size and target research quantity — get the exact draw volume in seconds.
More peptide calculation tools
The dosage calculator is just the start. Use the blend calculator to work out ratios when mixing two peptides in a research solution, or use the accumulation calculator to model how a peptide accumulates over a research cycle based on its half-life. All tools are for research purposes only.
