Healing
TB-500
Longevity
7
Amino acids
Molecular weight
Peptide
Type
TB-500 is a synthetic heptapeptide (Ac-LKKTETQ) corresponding to the actin-binding region of Thymosin Beta-4 (amino acids 17–23). It promotes tissue repair, wound healing, and angiogenesis by regulating actin polymerization and upregulating vascular endothelial growth factor (VEGF). Used primarily in research and by athletes seeking accelerated recovery from musculoskeletal injuries.
Top researched benefits
Overview of TB-500
TB-500 binds G-actin monomers to regulate actin polymerization, enabling increased cell motility, wound closure, and tissue remodeling. It also upregulates VEGF expression (2.5–3.8-fold in various cell types) to stimulate angiogenesis and inhibits NF-κB signaling to reduce inflammation at injury sites.
tissue repair
- Accelerated healing of muscle fibers with reduced recovery time and enhanced repair via cell migration and differentiation.
- Connective tissue repair with reduced scar formation and improved biomechanical properties of healing tissues.
- Enhanced dermal closure, improved angiogenesis, and reduced inflammation in acute and chronic scenarios.
athletic recovery
- Faster recovery from intense training with reduced muscle soreness and improved tissue repair.
- Strengthened tissues through enhanced repair mechanisms may reduce injury risk.
- Improved muscle quality through enhanced regeneration and repair pathways.
neurological
- Protective effects against neuronal damage in various models.
- Preclinical evidence for improved outcomes in spinal injury models.
- Potential neuroprotective and regenerative effects on brain tissue.
Typical Dose
2–2.5mg per injection
Frequency
twice weekly (loading phase), once weekly (maintenance)
Cycle Duration
4–6 weeks loading, then maintenance as needed
Storage
Lyophilized: -20°C in dry, dark conditions. Reconstituted with bacteriostatic water: 2–8°C for up to 28 days; do not freeze reconstituted solution.
Chemical Makeup
Key benefits
Accelerates healing of tendons, ligaments, and muscles by upregulating actin-mediated cell migration at injury sites
Promotes new blood vessel formation (angiogenesis) via 2.5–3.8-fold upregulation of VEGF, improving oxygen delivery to damaged tissue
Reduces inflammation by inhibiting NF-κB signaling and blocking TNF-α-induced inflammatory cascades
Mobilizes endogenous stem and progenitor cells from tissue niches and recruits them to sites of damage for regeneration
Community interest
This peptide is still gaining traction in the community.
Actin-Binding Fragment | Tissue Repair & Recovery
This overview is informational and based on aggregated descriptions from studies and user reports.
Was it helpful?YesNoTB-500 Molecular Information
View the scientifc details of TB-500.
7
Amino Acids
TB-500
Leu
Leu
Position 1
Lys
Lys
Position 2
Lys
Lys
Position 3
Thr
Thr
Position 4
Glu
Glu
Position 5
Thr
Thr
Position 6
Gln
Gln
Position 7
Molecular Weight
889.02DaChain Length
7Amino AcidsType
PeptideTB-500 Protocols
Subcutaneous injection is the primary and most established route for TB-500, delivering the peptide systemically regardless of injection site due to its mechanism of action via actin regulation and angiogenesis. Injections can be administered into the abdomen, thigh, or deltoid, with no strict time-of-day requirement.
| Goal | Dosage | Frequency | Route |
|---|---|---|---|
| Acute Injury Recovery | 7 | 2 week range | SubQ |
| Acute Injury Recovery — Maintenance | 3 | 1 week range | SubQ |
| Chronic Joint and Tendon Pain | 5 | 2 week range | SubQ |
| Chronic Joint and Tendon Pain — Maintenance | 2.5 | 1 week range | SubQ |
| Post-Surgical Recovery | 7 | 2 week range | SubQ |
| Injury Prevention and Performance | 3 | 2 month range | SubQ |
Reconstitution Instructions
Materials needed:
Steps to reconstitute
- Remove the TB-500 vial from the refrigerator and allow it to reach room temperature before opening to prevent condensation
- Swab the rubber stopper of both the TB-500 vial and the bacteriostatic water vial with an alcohol swab and allow to air dry completely
- Draw the desired volume of bacteriostatic water into an insulin syringe — a common ratio is 2 mL BAC water into a 10 mg vial (5 mg/mL concentration)
- Insert the needle through the rubber stopper at an angle and inject the bacteriostatic water slowly down the inside wall of the vial — do not inject directly onto the powder
- Gently roll the vial between your palms for 30–60 seconds until fully dissolved — do not shake or vortex
- Inspect the solution — it should be clear and colorless to pale yellow; discard if cloudy, discolored, or contains visible particles
- Store the reconstituted vial upright at 2–8°C (refrigerator) for up to 28 days when using bacteriostatic water
- For each injection, draw the calculated dose volume into a fresh insulin syringe, swab the injection site with alcohol, and allow to dry
- Pinch a skinfold at the injection site and insert the needle at 45–90 degrees into subcutaneous tissue
- Inject slowly and steadily — do not aspirate for subcutaneous injections — then dispose of the syringe in a sharps container
TB-500 Cycle
The TB-500 Cycle section explains how long a typical cycle lasts and what to expect during each phase. Over time, your body can become less responsive with continuous use.
Taking breaks between cycles may help maintain effectiveness and support better overall results.
- Week 1-2
- Reduced inflammation and improved tissue sensation
- Week 2-4
- Accelerated healing rate and improved tissue quality
- Week 4-8
- Maximum tissue repair and regenerative benefits
- Week 6-12
- Continued improvement in tissue strength and function
Dosing tools
TB-500 Peptide Dosage Calculator
Calculate peptide doses with our visual syringe guide.
mg
Enter the total amount of peptide in the vial in milligrams (as stated on the label).
The dose you want to inject per administration, in mcg or mg.
1,000 mcg = 1 mg
1 mL
2 mL
3 mL
5 mL
Custom
Volume of bacteriostatic water you add to reconstitute the powder. Use BAC water for preservation.
Injection Results
Based on your vial and dilution inputs.
TB-500
SINGLE COMPOUNDVolume per injection
0.05
mL
Concentration
10.00
mg/mL
Doses per vial
20
doses
Total injections per vial
20 injections
How it works
Based on a 10 mg TB-500 vial diluted with 1 mL of bacteriostatic water, each 500 mcg injection equals 0.05 mL.
1mL / 100 units
5 units
0.050 mL
Reference Guide
Dosing Cycle
- Peptide
- TB-500
- Dosing
- 2–2.5mg per injection
- Dosing Frequency
- twice weekly (loading phase), once weekly (maintenance)
- Cycle Duration
- 4–6 weeks loading, then maintenance as needed
- Storage
- Lyophilized: -20°C in dry, dark conditions. Reconstituted with bacteriostatic water: 2–8°C for up to 28 days; do not freeze reconstituted solution.
Note: Triple agonist; microdose for fewer side effects
Reconstitution Tips
- Use bacteriostatic water (BAC) — contains 0.9% benzyl alcohol for preservation
- Inject water slowly — aim down the vial wall, not directly onto powder
- Never shake — gently swirl or roll the vial until dissolved
- Store properly — refrigerate at 2-8°C after reconstitution
- Use within 28 days — most reconstituted peptides remain stable for about 4 weeks
- Keep sterile — always clean vial tops with alcohol before drawing
Peptide Interactions
Research suggestions of TB-500 interactions with other common peptides and substances.
Healing
bpc
Longevity
BPC-157
SYNERGISTIC
Complementary healing mechanisms with enhanced tissue repair when combined (Wolverine Stack).
Weight
ipa
Growth
Ipamorelin
COMPATIBLE
May enhance tissue sensitivity to growth hormone effects.
Side effects
Contraindications
Active cancer treatment (due to angiogenic effects)
Pregnancy or breastfeeding
Immunosuppressive medications (consult provider)
WADA prohibited for competitive athletes
Stop signs
Persistent or worsening injection site infections
Unusual swelling or prolonged redness
Severe allergic reactions
Unexpected systemic symptoms
Concerning health changes
Bad signs
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Frequently asked questions
What is a peptide dosage calculator?
A peptide dosage calculator is a free tool that converts your vial size, bacteriostatic water volume, and target dose into an exact syringe draw volume. Instead of doing the reconstitution math by hand, you enter three inputs and instantly get the concentration of your solution and how many milliliters or syringeunits to draw. This calculator works for single peptide compounds and multi-peptide blends.
How do I calculate peptide dosage from a vial?
To calculate your peptide dose, divide the total peptide content of your vial in micrograms by the volume of bacteriostatic water you added in milliliters. This gives you your solution concentration in mcg/mL. Then divide your target dose by that concentration to get your draw volume. For example, a 5mg (5,000 mcg) vial reconstituted with 2mL of BAC water gives a concentration of 2,500 mcg/mL. A 250 mcg dose would require drawing 0.1mL. This calculator automates all of those steps instantly.
How much Bacteriostatic water should I add to a peptide vial?
Most people add 2mL to 3mL of bacteriostatic water per vial, but the right amount depends on the dose you want to draw and the syringe size you are using. Adding 1mL to a 5mg vial gives you a concentration of 5,000 mcg/mL, making each dose very small in volume. Adding 2mL gives you 2,500 mcg/mL, which is easier to measure on a standard insulin syringe. A general guideline is to choose a volume that puts your typical dose somewhere between 10 and 30 units on a U-100 syringe. Use the calculator above to test different water volumes and find what works for your dose.
How are peptides different from proteins?
Both are made of amino acids, but peptides are much smaller than proteins. Because of their tiny size, peptides can act like tiny messengers in the body, sending specific signals to your cells to tell them exactly what to do.
TB-500Research References
TB-500 is a preclinical compound
TB-500
TB-500 is a preclinical compound
Dermal Wound Healing Study
Significant acceleration in wound healing rates in patients with chronic pressure ulcers and venous stasis ulcers compared to placebo in Phase 2 human trial.
2012
Cardiac Tissue Regeneration
TB-500 pre-treated endothelial cells improved left ventricular function by greater than 50% in post-MI subjects in human clinical trial.
2016
Muscle Fiber Regeneration
Improved skeletal muscle fiber regeneration in dystrophin-deficient mice, showing potential for muscular dystrophy treatment.
2015
Corneal Healing Research
Topical TB-500 significantly improved signs and symptoms of moderate to severe dry eye with lasting effects in Phase 2 human trial.
2014
Calculate peptide dosages
Learning how to calculate a peptide dose? Use our beginner-friendly peptide dosage, blend, and accumulation calculators. Enter vial size, reconstitution volume, and target dose to get exact draw volumes instantly. No guesswork, just clear guidance that helps prevent common mistakes.
