Healing
TB-500
Longevity
7
Amino acids
Molecular weight
Peptide
Type
A synthetic 7-amino acid fragment corresponding to the actin-binding region of thymosin beta-4, originally developed for equine use with retained tissue repair properties. TB-500 acts as principal actin-sequestering protein, regulating cell migration, promoting angiogenesis, reducing inflammation, and activating stem cell differentiation.
Top researched benefits
Overview of TB-500
Acts as principal actin-sequestering protein, regulating cell migration, promoting angiogenesis, reducing inflammation, and activating stem cell differentiation for comprehensive regenerative effects.
tissue repair
- Accelerated healing of muscle fibers with reduced recovery time and enhanced repair via cell migration and differentiation.
- Connective tissue repair with reduced scar formation and improved biomechanical properties of healing tissues.
- Enhanced dermal closure, improved angiogenesis, and reduced inflammation in acute and chronic scenarios.
athletic recovery
- Faster recovery from intense training with reduced muscle soreness and improved tissue repair.
- Strengthened tissues through enhanced repair mechanisms may reduce injury risk.
- Improved muscle quality through enhanced regeneration and repair pathways.
neurological
- Protective effects against neuronal damage in various models.
- Preclinical evidence for improved outcomes in spinal injury models.
- Potential neuroprotective and regenerative effects on brain tissue.
Typical Dose
2-5mg per injection (higher doses for serious injuries)
Frequency
2-3x weekly (e.g., Monday, Wednesday, Friday)
Cycle Duration
6-8 weeks for active healing
Storage
Reconstituted: 2-8°C, use within 28 days
Chemical Makeup
Key benefits
Superior systemic tissue repair
Accelerated muscle, tendon, and ligament healing
Enhanced cell migration and angiogenesis
Comprehensive regenerative effects
Neuroprotective properties
Community interest
This peptide is still gaining traction in the community.
Synthetic Fragment (17-23) of Thymosin Beta-4
This overview is informational and based on aggregated descriptions from studies and user reports.
Was it helpful?YesNoTB-500 Molecular Information
View the scientifc details of TB-500.
7
Amino Acids
TB-500
Leu
Leu
Position 1
Lys
Lys
Position 2
Lys
Lys
Position 3
Thr
Thr
Position 4
Glu
Glu
Position 5
Thr
Thr
Position 6
Gln
Gln
Position 7
Molecular Weight
949.1DaChain Length
7Amino AcidsType
PeptideTB-500 Protocols
Subcutaneous or intramuscular injection. Flexible timing with no empty stomach requirement. Can be injected near injury site for localized effect.
| Goal | Dosage | Frequency | Route |
|---|---|---|---|
| General tissue repair | 2 | 2 week range | SubQ or IM |
| Serious injury recovery | 4 | 3 week range | SubQ near injury site |
| Athletic enhancement | 2 | 2 week range | SubQ |
| Chronic conditions | 3 | 2 week range | SubQ or IM |
| Maintenance | 2 | 1 week range | SubQ |
| Post-surgical recovery | 3 | 3 week range | SubQ |
Reconstitution Instructions
Materials needed:
Steps to reconstitute
- Clean work area and hands thoroughly with alcohol
- Calculate required BAC water volume
- Draw calculated BAC water slowly into syringe
- Inject BAC water slowly down vial side (never directly on powder)
- Gently swirl vial until powder completely dissolves
- Store reconstituted solution refrigerated, use within 28 days
TB-500 Cycle
The TB-500 Cycle section explains how long a typical cycle lasts and what to expect during each phase. Over time, your body can become less responsive with continuous use.
Taking breaks between cycles may help maintain effectiveness and support better overall results.
- Week 1-2
- Reduced inflammation and improved tissue sensation
- Week 2-4
- Accelerated healing rate and improved tissue quality
- Week 4-8
- Maximum tissue repair and regenerative benefits
- Week 6-12
- Continued improvement in tissue strength and function
Dosing tools
TB-500 Peptide Dosage Calculator
Calculate peptide doses with our visual syringe guide.
mg
Enter the total amount of peptide in the vial in milligrams (as stated on the label).
The dose you want to inject per administration, in mcg or mg.
1,000 mcg = 1 mg
1 mL
2 mL
3 mL
5 mL
Custom
Volume of bacteriostatic water you add to reconstitute the powder. Use BAC water for preservation.
Injection Results
Based on your vial and dilution inputs.
TB-500
SINGLE COMPOUNDVolume per injection
0.05
mL
Concentration
10.00
mg/mL
Doses per vial
20
doses
Total injections per vial
20 injections
How it works
Based on a 10 mg TB-500 vial diluted with 1 mL of bacteriostatic water, each 500 mcg injection equals 0.05 mL.
1mL / 100 units
5 units
0.050 mL
Reference Guide
Dosing Cycle
- Peptide
- TB-500
- Dosing
- 2-5mg per injection (higher doses for serious injuries)
- Dosing Frequency
- 2-3x weekly (e.g., Monday, Wednesday, Friday)
- Cycle Duration
- 6-8 weeks for active healing
- Storage
- Reconstituted: 2-8°C, use within 28 days
Note: Triple agonist; microdose for fewer side effects
Reconstitution Tips
- Use bacteriostatic water (BAC) — contains 0.9% benzyl alcohol for preservation
- Inject water slowly — aim down the vial wall, not directly onto powder
- Never shake — gently swirl or roll the vial until dissolved
- Store properly — refrigerate at 2-8°C after reconstitution
- Use within 28 days — most reconstituted peptides remain stable for about 4 weeks
- Keep sterile — always clean vial tops with alcohol before drawing
Peptide Interactions
Research suggestions of TB-500 interactions with other common peptides and substances.
Healing
bpc
Longevity
BPC-157
SYNERGISTIC
Complementary healing mechanisms with enhanced tissue repair when combined (Wolverine Stack).
Weight
ipa
Growth
Ipamorelin
COMPATIBLE
May enhance tissue sensitivity to growth hormone effects.
Side effects
Contraindications
Active cancer treatment (due to angiogenic effects)
Pregnancy or breastfeeding
Immunosuppressive medications (consult provider)
WADA prohibited for competitive athletes
Stop signs
Persistent or worsening injection site infections
Unusual swelling or prolonged redness
Severe allergic reactions
Unexpected systemic symptoms
Concerning health changes
Bad signs
Comments
0.0
0 reviews
5
4
3
2
1
No comments yet
Be the first to share your experience. Your review helps others make more informed decisions.
Frequently asked questions
What is a peptide dosage calculator?
A peptide dosage calculator is a free tool that converts your vial size, bacteriostatic water volume, and target dose into an exact syringe draw volume. Instead of doing the reconstitution math by hand, you enter three inputs and instantly get the concentration of your solution and how many milliliters or syringeunits to draw. This calculator works for single peptide compounds and multi-peptide blends.
How do I calculate peptide dosage from a vial?
To calculate your peptide dose, divide the total peptide content of your vial in micrograms by the volume of bacteriostatic water you added in milliliters. This gives you your solution concentration in mcg/mL. Then divide your target dose by that concentration to get your draw volume. For example, a 5mg (5,000 mcg) vial reconstituted with 2mL of BAC water gives a concentration of 2,500 mcg/mL. A 250 mcg dose would require drawing 0.1mL. This calculator automates all of those steps instantly.
How much Bacteriostatic water should I add to a peptide vial?
Most people add 2mL to 3mL of bacteriostatic water per vial, but the right amount depends on the dose you want to draw and the syringe size you are using. Adding 1mL to a 5mg vial gives you a concentration of 5,000 mcg/mL, making each dose very small in volume. Adding 2mL gives you 2,500 mcg/mL, which is easier to measure on a standard insulin syringe. A general guideline is to choose a volume that puts your typical dose somewhere between 10 and 30 units on a U-100 syringe. Use the calculator above to test different water volumes and find what works for your dose.
How are peptides different from proteins?
Both are made of amino acids, but peptides are much smaller than proteins. Because of their tiny size, peptides can act like tiny messengers in the body, sending specific signals to your cells to tell them exactly what to do.
TB-500Research References
TB-500 is a well studied compound
TB-500
TB-500 is a well studied compound
Dermal Wound Healing Study
Significant acceleration in wound healing rates in patients with chronic pressure ulcers and venous stasis ulcers compared to placebo in Phase 2 human trial.
2012
Cardiac Tissue Regeneration
TB-500 pre-treated endothelial cells improved left ventricular function by greater than 50% in post-MI subjects in human clinical trial.
2016
Muscle Fiber Regeneration
Improved skeletal muscle fiber regeneration in dystrophin-deficient mice, showing potential for muscular dystrophy treatment.
2015
Corneal Healing Research
Topical TB-500 significantly improved signs and symptoms of moderate to severe dry eye with lasting effects in Phase 2 human trial.
2014