Reviewed by the PeptideMind Team · Updated August 5, 2026

TB-500 Peptide Dosage Calculator, Side Effects & Legal Status

Use our free TB-500 peptide (Thymosin Beta-4 fragment) dosage calculator to get precise injection protocols, cycle length, and reconstitution amounts for tendon, muscle & injury recovery. See benefits, side effects & legal status.

Basic Dosage Calculator

Calculate peptide doses with our visual syringe guide.

Peptide per vial

Target research quantity

Bac water added

As seen on

TB-500 Dosage Schedule

Starts with a higher frequency loading phase to jumpstart tissue repair, then steps down to a lower, ongoing maintenance dose once the initial recovery gains are established.

Loading Then Maintenance Recovery Protocol

an ongoing run with no fixed end date with the dose increasing over time, focused on healing and recovery.

2 days per week

Taken Monday and Thursday.

2–3 mg

Starts at 2 mg and increases to 3 mg over 2 phases as your body adjusts. Injected just under the skin in the abdomen or thigh.

TB-500 helps regenerate tissue and reduce inflammation. The 4-6 week schedule is a loading phase; after that, protocols typically drop to 2-4 mg every one to two weeks for maintenance.

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What is TB-500?

TB-500 is a lab-made peptide based on a small fragment of a natural repair protein found throughout the body (Thymosin Beta-4). It works by helping regulate how cells rebuild their internal structure and by boosting a key blood vessel growth signal, both of which support faster healing. It's used mainly in research and by athletes seeking faster recovery from injuries to muscles, tendons, and ligaments.

Healing

Research targets include tissue repair, wound healing, and recovery from injury.

Longevity

Research targets include cellular aging, senescence, and long-term healthspan.

Healing

tb5

Longevity

Where to buy TB-500

Compare real-time TB-500 prices from vendors with third-party lab testing (COA), purity, and legitimacy, so you know exactly who's trustworthy before you buy.

Dose size

5mgfrom $34 · 9 vendors10mgfrom $40 · 10 vendors2mgfrom $48 · 1 vendor

Peptide Mind's top pick

Chosen on COA purity, third-party testing, and service, no paid placements.

Protide Health

99.27% COA|5.0|In stock

Buy now at Protide Health Compared across 13 vendors

8 of 13 vendors third-party tested, COA purity 99–99.97% across published labs. Prices re-checked 17 days ago.

What Does TB-500 Do?

It binds to a key structural protein inside cells (actin), helping regulate how cells rebuild their internal scaffolding so they can move and close wounds more efficiently. It also boosts a molecule that triggers new blood vessel growth and helps quiet inflammatory signaling at the injury site.

Binds actin

Regulates a key structural protein inside cells.

Helps cells move

Lets cells rebuild their scaffolding to move and close wounds.

Grows vessels, calms inflammation

Boosts a vessel-growth molecule and quiets inflammatory signaling.

Frequently Asked Questions About TB-500

Straight answers on reconstitution, dosing, and safety, everything you need to research with confidence. For research reference only.

TB-500 is a synthetic peptide derived from thymosin beta-4 (Tβ4), a naturally occurring protein found throughout the body that plays a role in cell migration, blood vessel formation, and tissue repair. It is studied primarily for its potential to support healing of tendons, ligaments, muscles, and skin by upregulating actin, a protein essential for cell movement and tissue remodeling. Unlike BPC-157, which is thought to act more locally at an injury site, TB-500 is generally described as working more systemically throughout the body.

Commonly cited research protocols use a 'loading phase' of roughly 4-5 mg total per week (often split into 2-3 subcutaneous or intramuscular injections of about 2 mg each) for 4-6 weeks, followed by a lower maintenance dose of around 2-4 mg per week if use continues. These figures come from informal research and vendor/community sources rather than clinical trials, and no standardized human dosing exists because TB-500 has not gone through FDA-regulated dose-finding studies.

Most commonly reported effects are mild and transient, including headache (especially in the first 1-2 weeks), temporary redness or irritation at the injection site, and occasional fatigue after early doses. Less common but more serious reported reactions include fever, rash, hives, severe itching, vomiting, or blistering at the injection site, in which case discontinuing use and seeking medical attention is generally recommended. Because TB-500 has not been through formal human clinical trials, the full side-effect profile, long-term safety, and rare-event rates are not well established.

TB-500 is not approved by the FDA (or equivalent regulators) for human use and has not completed clinical trials. It is typically sold labeled 'for research use only, not for human consumption,' and it is legal to purchase and possess in that context in the US, but using it therapeutically on oneself falls outside approved/regulated use. TB-500 (like thymosin beta-4) is also included on the World Anti-Doping Agency's prohibited list, so it is banned for athletes subject to anti-doping testing.

TB-500 is typically supplied as a lyophilized (freeze-dried) powder that is reconstituted with bacteriostatic or sterile water before use. Once reconstituted, it is generally recommended to store the solution refrigerated (2-8°C) and protected from light, and to avoid freezing, as ice crystals can damage the peptide structure. Vials mixed with bacteriostatic water (which contains a preservative) are commonly cited as stable for up to about 28 days refrigerated, while water without a preservative shortens the usable window considerably. Unreconstituted lyophilized powder is generally more stable and can often be stored frozen for longer periods until mixed.

TB-500 and BPC-157 are frequently discussed together as the 'Wolverine stack' because they are believed to act through complementary mechanisms: BPC-157 is described as promoting localized repair and blood vessel growth at a specific injury site, while TB-500 is described as supporting broader, systemic cell migration and tissue regeneration. Advocates suggest this combination may address both the local and systemic aspects of soft-tissue healing, though this combined use has not been validated in controlled human studies.

Sources commonly advise against TB-500 use by individuals with active cancer or a history of certain cancers, since TB-500 promotes cell migration and proliferation, which could theoretically support tumor growth or metastasis, though this concern is largely theoretical rather than demonstrated in human trials. As with any unapproved research compound, anyone with an existing medical condition, taking other medications, or considering use should be aware that safety in these contexts has not been formally studied.

TB-500 is generally administered by subcutaneous or intramuscular injection rather than orally, since peptides are broken down in the digestive tract before they could take effect. Some protocols suggest injecting near the area of injury (e.g., near a strained tendon) may be preferred by some users for localized effect, while systemic subcutaneous injection elsewhere on the body is also commonly used given TB-500's proposed systemic mechanism. There is no strong clinical evidence establishing an optimal injection site.

TB-500 Reconstitution Guide

TB-500 is supplied as a freeze-dried powder that must be mixed into a injectable solution before use. Available in 2mg, 5mg, 10mg sizes.

10 mg lyophilized vial

TB-500 is supplied as a freeze-dried powder that must be mixed into a injectable solution before use. Available in 2mg, 5mg, 10mg sizes.

3 mL bacteriostatic water

Adding 3 mL of bac water to a 10 mg vial gives a workable concentration for syringe dosing. More water makes microdosing easier; less concentrates each draw.

Storage after mixing

Freeze the dry powder somewhere dry and dark. Once mixed with bacteriostatic water, refrigerate and use within about a month, and don't freeze it once mixed. After reconstitution, keep the vial refrigerated and use within 30 days.

Reconstitution steps

Remove caps & sanitize stoppers

Attach needle to syringe

Draw 3 mL bacteriostatic water

Insert needle into TB-500 vial

Release water gently down the side

Swirl to dissolve — don't shake

Check for full dissolution

Refrigerate TB-500 — good for 30 days

Chemical Makeup

Amino Acids

TB-500

Ser

Position 1

Asp

Position 2

Lys

Position 3

Pro

Position 4

Asp

Position 5

Met

Position 6

Ala

Position 7

Glu

Position 8

Ile

Position 9

Glu

Position 10

Lys

Position 11

Phe

Position 12

Asp

Position 13

Lys

Position 14

Ser

Position 15

Lys

Position 16

Leu

Position 17

Lys

Position 18

Lys

Position 19

Thr

Position 20

Glu

Position 21

Thr

Position 22

Gln

Position 23

Glu

Position 24

Lys

Position 25

Asn

Position 26

Pro

Position 27

Leu

Position 28

Pro

Position 29

Ser

Position 30

Lys

Position 31

Glu

Position 32

Thr

Position 33

Ile

Position 34

Glu

Position 35

Gln

Position 36

Glu

Position 37

Lys

Position 38

Gln

Position 39

Ala

Position 40

Gly

Position 41

Glu

Position 42

Ser

Position 43

Amino acid sequence

SerinePosition 1

Aspartic acidPosition 2

LysinePosition 3

ProlinePosition 4

Aspartic acidPosition 5

MethioninePosition 6

AlaninePosition 7

Glutamic acidPosition 8

IsoleucinePosition 9

Glutamic acidPosition 10

LysinePosition 11

PhenylalaninePosition 12

Aspartic acidPosition 13

LysinePosition 14

SerinePosition 15

LysinePosition 16

LeucinePosition 17

LysinePosition 18

LysinePosition 19

ThreoninePosition 20

Glutamic acidPosition 21

ThreoninePosition 22

GlutaminePosition 23

Glutamic acidPosition 24

LysinePosition 25

AsparaginePosition 26

ProlinePosition 27

LeucinePosition 28

ProlinePosition 29

SerinePosition 30

LysinePosition 31

Glutamic acidPosition 32

ThreoninePosition 33

IsoleucinePosition 34

Glutamic acidPosition 35

GlutaminePosition 36

Glutamic acidPosition 37

LysinePosition 38

GlutaminePosition 39

AlaninePosition 40

GlycinePosition 41

Glutamic acidPosition 42

SerinePosition 43

What Are the Benefits of TB-500?

What research says it may help with, and how it works in the body.

tissue repair

: It speeds up healing of muscle fibers and shortens recovery time by helping repair cells travel to the injury site and mature into the cell types needed to fix it.
: It helps skin wounds close faster, encourages new blood vessel growth to support healing, and reduces inflammation in both sudden injuries and long-lasting wounds.
: It helps repair connective tissue (like tendons and ligaments) with less scarring, and the healed tissue ends up stronger and more functional.

athletic recovery

: It helps you bounce back faster from intense workouts, with less muscle soreness and better tissue repair.
: By boosting the body's own repair processes, it may strengthen tissues and lower the risk of injury.
: It improves the quality of muscle tissue by boosting the body's natural processes for regenerating and repairing it.

neurological

: In various research models, it protected nerve cells from damage.
: Early lab and animal research shows it improves outcomes after spinal cord injury.
: It may protect brain tissue from damage and help it regenerate.

What Are the Side Effects of TB-500?

Who should avoid it, warning signs to watch for, and what to know before combining it with other compounds.

Who Should Avoid It

: Active cancer treatment (due to angiogenic effects)
: Pregnancy or breastfeeding
: Immunosuppressive medications (consult provider)
: WADA prohibited for competitive athletes

Stop Right Away If You Notice

: Persistent or worsening injection site infections
: Unusual swelling or prolonged redness
: Severe allergic reactions
: Unexpected systemic symptoms
: Concerning health changes

Milder Signs to Watch For

Colored or crystalline powder (yellow/brown indicates degradation)Persistent cloudiness, floating particles, or precipitationDamaged vial seal or contamination signs

Was it helpful?Yes

Is TB-500 FDA approved?

TB-500 is not an FDA-approved drug. It is intended for research purposes only and is not approved for human consumption, diagnostic, or therapeutic use.

Research Use Only

TB-500 has not been evaluated by the FDA for safety or efficacy in humans.

No Clinical Oversight

Not manufactured under FDA-regulated quality or clinical standards.

Unregulated Sourcing

Purity, dosing, and sourcing are not verified through FDA testing or oversight.

A smartphone screen displaying FDA status for a drug, indicating "FDA approved drug: No" and "Classification: Research use only." A banner notes "Research Only" with the disclaimer "not for human use," emphasizing its intended use for laboratory and analytical purposes.

TB-500 Cycle Length: How Long Should It Last?

This breaks down how long a typical TB-500 cycle runs and what research suggests happens at each stage. Research shows that staying on a peptide continuously, without a break, may make it less effective over time.

That's why most research protocols build in a break between cycles, often called a washout period, to let the body reset before starting again.

Week 1-2: Reduced inflammation and improved tissue sensation
Week 2-4: Accelerated healing rate and improved tissue quality
Week 4-8: Maximum tissue repair and regenerative benefits
Week 6-12: Continued improvement in tissue strength and function

TB-500 Research References

It is a preclinical compound

4Research references

TB-500

TB-500 is a preclinical compound

Cardiac Tissue Regeneration

TB-500 pre-treated endothelial cells improved left ventricular function by greater than 50% in post-MI subjects in human clinical trial.

2016

Muscle Fiber Regeneration

Improved skeletal muscle fiber regeneration in dystrophin-deficient mice, showing potential for muscular dystrophy treatment.

2015

Dermal Wound Healing Study

Significant acceleration in wound healing rates in patients with chronic pressure ulcers and venous stasis ulcers compared to placebo in Phase 2 human trial.

2012

Corneal Healing Research

Topical TB-500 significantly improved signs and symptoms of moderate to severe dry eye with lasting effects in Phase 2 human trial.

2014

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BPC-157 vs TB-500: The Wolverine peptide stack comparison

BPC-157 acts locally at the site of injury. TB-500 acts systemically through the bloodstream. Here's how BPC-157 and TB-500 compare, and why they're often combined into the Wolverine Stack.

TB-500 Peptide Dosage Calculator, Side Effects & Legal Status

TB-500 Dosage Schedule

What is TB-500?

Where to buy TB-500

Dose size

What Does TB-500 Do?

Frequently Asked Questions About TB-500

What is TB-500 and how does it work?

What dosage and injection frequency are typically used in research protocols?

What side effects have been reported?

Is TB-500 legal, and is it approved for human use?

How is TB-500 reconstituted and stored?

Why do people combine TB-500 with BPC-157 (the 'Wolverine stack')?

Should TB-500 be avoided by anyone in particular?

How is TB-500 typically administered, and does injection site matter?

TB-500 Reconstitution Guide

Chemical Makeup

What Are the Benefits of TB-500?

tissue repair

athletic recovery

neurological

What Are the Side Effects of TB-500?

Who Should Avoid It

Stop Right Away If You Notice

Milder Signs to Watch For

Glow Blend

Klow Blend

Coremend Blend

Wolverine Blend

Is TB-500 FDA approved?

TB-500 Cycle Length: How Long Should It Last?

TB-500 Research References

Browse user-created protocols

TB-500 Comparisons

BPC-157 vs TB-500: The Wolverine peptide stack comparison

TB-500 Peptide Dosage Calculator, Side Effects & Legal Status

TB-500 Dosage Schedule

What is TB-500?

Where to buy TB-500

Dose size

What Does TB-500 Do?

TB-500 Targeted areas

Frequently Asked Questions About TB-500

What is TB-500 and how does it work?

What dosage and injection frequency are typically used in research protocols?

What side effects have been reported?

Is TB-500 legal, and is it approved for human use?

How is TB-500 reconstituted and stored?

Why do people combine TB-500 with BPC-157 (the 'Wolverine stack')?

Should TB-500 be avoided by anyone in particular?

How is TB-500 typically administered, and does injection site matter?

TB-500 Reconstitution Guide

Chemical Makeup

What Are the Benefits of TB-500?

tissue repair

athletic recovery

neurological

What Are the Side Effects of TB-500?

Who Should Avoid It

Stop Right Away If You Notice

Milder Signs to Watch For

Peptides Commonly Stacked With TB-500

Glow Blend

Klow Blend

Coremend Blend

Wolverine Blend

Is TB-500 FDA approved?

TB-500 Cycle Length: How Long Should It Last?

TB-500 Research References

Related peptides

Browse user-created protocols

TB-500 Comparisons

BPC-157 vs TB-500: The Wolverine peptide stack comparison