Reviewed by the PeptideMind Team · Updated July 15, 2026
TB-500 Dosage Guide, Side Effects & Legal Status
TB-500 upregulates VEGF and mobilizes stem cells to accelerate tendon, muscle, and corneal healing while reducing inflammation. See dosing.
Chemical Makeup
7
Amino Acids
TB-500
Leu
LPosition 1
Lys
KPosition 2
Lys
KPosition 3
Thr
TPosition 4
Glu
EPosition 5
Thr
TPosition 6
Gln
QPosition 7
As seen on
TB-500 Dosage Guide for Recovery & RepairTB-500 Dosage Calculator
TB-500 helps regenerate tissue and reduce inflammation.
Vial size
10 mg
Bacteriostatic water
3 mL
Dosing
1–2 mg
Frequency
Every 2 days
Cycle
4–6 weeks
Benefit
Recovery & Repair
What is TB-500?
TB-500 is a lab-made peptide based on a small fragment of a natural repair protein found throughout the body (Thymosin Beta-4). It works by helping regulate how cells rebuild their internal structure and by boosting a key blood vessel growth signal, both of which support faster healing. It's used mainly in research and by athletes seeking faster recovery from injuries to muscles, tendons, and ligaments.
Healing
tb5
Longevity
Is TB-500 FDA approved?
TB-500 is not an FDA-approved drug. It is intended for research purposes only and is not approved for human consumption, diagnostic, or therapeutic use.
Research Use Only
TB-500 has not been evaluated by the FDA for safety or efficacy in humans.
No Clinical Oversight
Not manufactured under FDA-regulated quality or clinical standards.
Unregulated Sourcing
Purity, dosing, and sourcing are not verified through FDA testing or oversight.
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How Does TB-500 Work?
It binds to a key structural protein inside cells (actin), helping regulate how cells rebuild their internal scaffolding so they can move and close wounds more efficiently. It also boosts a molecule that triggers new blood vessel growth and helps quiet inflammatory signaling at the injury site.
Binds actin
Regulates a key structural protein inside cells.
Helps cells move
Lets cells rebuild their scaffolding to move and close wounds.
Grows vessels, calms inflammation
Boosts a vessel-growth molecule and quiets inflammatory signaling.
TB-500 Targeted areas
How to reconstitute TB-500
View guideThe materials you'll need and step-by-step instructions for safely mixing TB-500 with bacteriostatic water.
Materials needed
Your TB-500 vial (lyophilized)
Alcohol swabs
Bacteriostatic sterile water
3 mL syringes (Luer Lock tip)
25G or 27G needles (Luer Lock). Other gauges may also be acceptable.
Sharps container (optional)
Remove the caps
Sanitize the rubber stoppers
Attach the needle
Draw the bac water
Pull back on the plunger to draw your desired volume of bacteriostatic water. If you overfill, just push the excess back in until you reach the right marker on the syringe.
Insert the needle into the TB-500 vial
With the bac water in your syringe, insert the needle into the TB-500 vial at a slight angle to avoid pressure buildup.
Release the water gently
Let the water run gently down the side of the vial. Don't inject it forcefully.
Swirl to dissolve
Avoid shaking. Gently swirl, flip, and roll the vial to dissolve the powder.
Check for full dissolution
Cap, dispose, and store
How to Store TB-500
Freeze the dry powder somewhere dry and dark. Once mixed with bacteriostatic water, refrigerate and use within about a month, and don't freeze it once mixed.
Lyophilized Storage
-20°C in dry, dark conditions.
Reconstituted Storage
With bacteriostatic water, 2–8°C for up to 28 days.
Handling Notes
Do not freeze the reconstituted solution.
What Are the Benefits of TB-500?
What research says it may help with, and how it works in the body.
tissue repair
It speeds up healing of muscle fibers and shortens recovery time by helping repair cells travel to the injury site and mature into the cell types needed to fix it.
It helps skin wounds close faster, encourages new blood vessel growth to support healing, and reduces inflammation in both sudden injuries and long-lasting wounds.
It helps repair connective tissue (like tendons and ligaments) with less scarring, and the healed tissue ends up stronger and more functional.
athletic recovery
It helps you bounce back faster from intense workouts, with less muscle soreness and better tissue repair.
By boosting the body's own repair processes, it may strengthen tissues and lower the risk of injury.
It improves the quality of muscle tissue by boosting the body's natural processes for regenerating and repairing it.
neurological
In various research models, it protected nerve cells from damage.
Early lab and animal research shows it improves outcomes after spinal cord injury.
It may protect brain tissue from damage and help it regenerate.
What Are the Side Effects of TB-500?
Who should avoid it, warning signs to watch for, and what to know before combining it with other compounds.
Who Should Avoid It
Active cancer treatment (due to angiogenic effects)
Pregnancy or breastfeeding
Immunosuppressive medications (consult provider)
WADA prohibited for competitive athletes
Stop Right Away If You Notice
Persistent or worsening injection site infections
Unusual swelling or prolonged redness
Severe allergic reactions
Unexpected systemic symptoms
Concerning health changes
Milder Signs to Watch For
How Long Should a TB-500 Cycle Last?
This breaks down how long a typical TB-500 cycle runs and what research suggests happens at each stage. Research shows that staying on a peptide continuously, without a break, may make it less effective over time.
That's why most research protocols build in a break between cycles, often called a washout period, to let the body reset before starting again.
- Week 1-2
- Reduced inflammation and improved tissue sensation
- Week 2-4
- Accelerated healing rate and improved tissue quality
- Week 4-8
- Maximum tissue repair and regenerative benefits
- Week 6-12
- Continued improvement in tissue strength and function
Cheat Sheets Featuring TB-500
See how TB-500 fits into a broader research stack with these free downloadable cheat sheets. Each one includes reference dose ranges, dosing frequency, and half-life for every peptide it covers. For research purposes only.
Browse user-created protocols
Real protocols from the community that include TB-500
Healing
bpc
Healing
tb5
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Cognitive
ill
TB-500 Research References
It is a preclinical compound
TB-500
TB-500 is a preclinical compound
TB-500 pre-treated endothelial cells improved left ventricular function by greater than 50% in post-MI subjects in human clinical trial.
2016
Improved skeletal muscle fiber regeneration in dystrophin-deficient mice, showing potential for muscular dystrophy treatment.
2015
Significant acceleration in wound healing rates in patients with chronic pressure ulcers and venous stasis ulcers compared to placebo in Phase 2 human trial.
2012
Topical TB-500 significantly improved signs and symptoms of moderate to severe dry eye with lasting effects in Phase 2 human trial.
2014
Frequently Asked Questions About TB-500
Straight answers on reconstitution, dosing, and safety, everything you need to research with confidence. For research reference only.