Healing
Cardiogen
Longevity
4
Amino acids
Molecular weight
Peptide
Type
Cardiogen is a synthetic tetrapeptide (H-Ala-Glu-Asp-Arg-OH, AEDR) originally developed in Russia as a cardiac tissue bioregulator. It acts as an epigenetic regulator of gene expression in cardiomyocytes and cardiac fibroblasts, supporting myocardial cell survival, proliferation, and structural protein synthesis. It is used in preclinical research contexts and among longevity-focused individuals seeking cardiovascular tissue support.
Top researched benefits
Overview of Cardiogen
Cardiogen enters cardiomyocytes and modulates gene expression by upregulating structural proteins including lamin A/C, actin, vimentin, and tubulin while suppressing p53-mediated apoptosis in cardiac tissue. It promotes extracellular matrix remodeling by stimulating fibroblast synthesis of collagen and elastin, and may inhibit endonuclease-catalyzed DNA hydrolysis to preserve genomic integrity in stressed cardiac cells.
cardiovascular support
- Supports heart tissue repair through gene expression regulation at the nuclear level.
- May improve overall myocardial function and cardiac performance.
- Addresses age-related cardiovascular decline through bioregulation.
antiaging
- Influences gene expression to support tissue repair without altering DNA sequence.
- Promotes cardiac tissue regeneration at the cellular level.
Typical Dose
100–200mcg per injection
Frequency
once daily
Cycle Duration
10–20 day cycles, 1–3 times per year
Storage
Lyophilized: -20°C in dry, dark conditions. Reconstituted with bacteriostatic water: 2–8°C for up to 2–4 weeks. Avoid repeated freeze-thaw cycles.
Chemical Makeup
Key benefits
Stimulates cardiomyocyte proliferation and survival in both young and aged cardiac tissue, supporting myocardial regeneration after injury
Suppresses p53-mediated apoptosis in healthy cardiac cells, reducing unnecessary cell death during ischemic or inflammatory stress
Promotes fibroblast secretion of collagen and elastin to restore extracellular matrix integrity in damaged heart muscle
Upregulates cytoskeletal proteins (actin, vimentin, tubulin) and nuclear lamins to strengthen the structural framework of cardiac cells
Community interest
This peptide is still gaining traction in the community.
Cardiac Bioregulator | Heart Repair & Longevity
This overview is informational and based on aggregated descriptions from studies and user reports.
Was it helpful?YesNoCardiogen Molecular Information
View the scientifc details of Cardiogen.
4
Amino Acids
Cardiogen
Ala
Ala
Position 1
Glu
Glu
Position 2
Asp
Asp
Position 3
Arg
Arg
Position 4
Molecular Weight
489.5DaChain Length
4Amino AcidsType
PeptideCardiogen Protocols
Subcutaneous injection is the primary delivery route for Cardiogen (AEDR), offering direct systemic availability of this cardiac bioregulator tetrapeptide. Morning administration is preferred, with injection sites rotated across the abdomen, outer thighs, and upper arms across the 2–4 week cycle.
| Goal | Dosage | Frequency | Route |
|---|---|---|---|
| Maintain baseline cardiac function and support cardiovascular tissue resilience in otherwise healthy individuals | 250 | 5 week range | SubQ |
| Support myocardial repair signaling following ischemic event or significant cardiac stress | 500 | 5 week range | SubQ |
| Reduce cardiac remodeling markers during periods of sustained physical or metabolic stress | 300 | 5 week range | SubQ |
| Address active fibroblast signaling and tissue-repair pathways in cardiovascular disease research models | 500 | 7 week range | SubQ |
| Periodic low-dose administration to sustain Khavinson bioregulator cycling effects on cardiac gene expression across aging | 200 | 3 week range | SubQ |
Reconstitution Instructions
Materials needed:
Steps to reconstitute
- Allow the Cardiogen lyophilized powder vial to reach room temperature before opening
- Draw 2–3 mL of bacteriostatic water (BAC water) into a syringe — adding 2 mL to a 20 mg vial yields 10 mg/mL; adding 3 mL yields approximately 6.67 mg/mL
- Insert the needle through the rubber stopper of the vial and inject the BAC water slowly down the inner wall — do not squirt directly onto the powder
- Gently swirl the vial in a slow circular motion until the powder is fully dissolved — do not shake or vortex
- Inspect the solution — it should appear clear and colorless with no visible particulates before drawing a dose
- Store the reconstituted vial refrigerated at 2–8°C and use within 30 days; never freeze the reconstituted solution
- Calculate the volume for your dose: at 6.67 mg/mL (3 mL BAC water in a 20 mg vial), a 500 mcg dose requires drawing 0.075 mL; use an insulin syringe for accuracy
- Clean the injection site (abdomen at least 2 inches from navel, outer thigh, or upper arm) with an alcohol swab and allow it to dry fully
- Pinch the skin lightly, insert the needle at a 45–90 degree angle into subcutaneous fat, inject slowly, then withdraw and apply gentle pressure — do not rub the site
- Rotate injection sites with each administration to prevent lipohypertrophy
Cardiogen Cycle
The Cardiogen Cycle section explains how long a typical cycle lasts and what to expect during each phase. Over time, your body can become less responsive with continuous use.
Taking breaks between cycles may help maintain effectiveness and support better overall results.
- Week 0-0
- Gene expression modulation begins
- Week 0-0
- Effects persist due to epigenetic changes
- Week 0-0
- Cardiovascular function improvements
- Week 0-0
- Cumulative benefits with periodic cycles
Dosing tools
Cardiogen Peptide Dosage Calculator
Calculate peptide doses with our visual syringe guide.
mg
Enter the total amount of peptide in the vial in milligrams (as stated on the label).
The dose you want to inject per administration, in mcg or mg.
1,000 mcg = 1 mg
1 mL
2 mL
3 mL
5 mL
Custom
Volume of bacteriostatic water you add to reconstitute the powder. Use BAC water for preservation.
Injection Results
Based on your vial and dilution inputs.
Cardiogen
SINGLE COMPOUNDVolume per injection
0.05
mL
Concentration
10.00
mg/mL
Doses per vial
20
doses
Total injections per vial
20 injections
How it works
Based on a 10 mg Cardiogen vial diluted with 1 mL of bacteriostatic water, each 500 mcg injection equals 0.05 mL.
1mL / 100 units
5 units
0.050 mL
Reference Guide
Dosing Cycle
- Peptide
- Cardiogen
- Dosing
- 100–200mcg per injection
- Dosing Frequency
- once daily
- Cycle Duration
- 10–20 day cycles, 1–3 times per year
- Storage
- Lyophilized: -20°C in dry, dark conditions. Reconstituted with bacteriostatic water: 2–8°C for up to 2–4 weeks. Avoid repeated freeze-thaw cycles.
Note: Triple agonist; microdose for fewer side effects
Reconstitution Tips
- Use bacteriostatic water (BAC) — contains 0.9% benzyl alcohol for preservation
- Inject water slowly — aim down the vial wall, not directly onto powder
- Never shake — gently swirl or roll the vial until dissolved
- Store properly — refrigerate at 2-8°C after reconstitution
- Use within 28 days — most reconstituted peptides remain stable for about 4 weeks
- Keep sterile — always clean vial tops with alcohol before drawing
Peptide Interactions
Research suggestions of Cardiogen interactions with other common peptides and substances.
Longevity
epi
Immunity
Epitalon
SYNERGISTIC
Often combined in comprehensive anti-aging Khavinson protocols.
Cognitive
pin
Longevity
Pinealon
COMPATIBLE
Part of Khavinson bioregulator family; targets different tissue.
Longevity
thy
Immunity
Thymalin
COMPATIBLE
Different organ targets; can be used in comprehensive bioregulator protocols.
Healing
vesu
Longevity
Vesugen
SYNERGISTIC
Complementary cardiovascular bioregulators - Cardiogen for heart, Vesugen for vessels.
Side effects
Contraindications
Active cardiac emergencies (seek medical care)
Known hypersensitivity
Pregnancy or breastfeeding
Stop signs
Allergic reactions
Unusual cardiac symptoms
Bad signs
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Frequently asked questions
What is a peptide dosage calculator?
A peptide dosage calculator is a free tool that converts your vial size, bacteriostatic water volume, and target dose into an exact syringe draw volume. Instead of doing the reconstitution math by hand, you enter three inputs and instantly get the concentration of your solution and how many milliliters or syringeunits to draw. This calculator works for single peptide compounds and multi-peptide blends.
How do I calculate peptide dosage from a vial?
To calculate your peptide dose, divide the total peptide content of your vial in micrograms by the volume of bacteriostatic water you added in milliliters. This gives you your solution concentration in mcg/mL. Then divide your target dose by that concentration to get your draw volume. For example, a 5mg (5,000 mcg) vial reconstituted with 2mL of BAC water gives a concentration of 2,500 mcg/mL. A 250 mcg dose would require drawing 0.1mL. This calculator automates all of those steps instantly.
How much Bacteriostatic water should I add to a peptide vial?
Most people add 2mL to 3mL of bacteriostatic water per vial, but the right amount depends on the dose you want to draw and the syringe size you are using. Adding 1mL to a 5mg vial gives you a concentration of 5,000 mcg/mL, making each dose very small in volume. Adding 2mL gives you 2,500 mcg/mL, which is easier to measure on a standard insulin syringe. A general guideline is to choose a volume that puts your typical dose somewhere between 10 and 30 units on a U-100 syringe. Use the calculator above to test different water volumes and find what works for your dose.
How are peptides different from proteins?
Both are made of amino acids, but peptides are much smaller than proteins. Because of their tiny size, peptides can act like tiny messengers in the body, sending specific signals to your cells to tell them exactly what to do.
CardiogenResearch References
Cardiogen is a preclinical compound
Cardiogen
Cardiogen is a preclinical compound
Peptide Regulation of Gene Expression: A Systematic Review
Short peptides can penetrate nuclei, interact with DNA and histones to regulate gene expression.
n.d.
Cardiogen Bioregulator Research
Demonstrated effects on cardiac tissue through gene expression modulation.
n.d.
Khavinson Peptide Bioregulators Overview
Comprehensive review of bioregulator peptides and their epigenetic mechanisms.
n.d.
Calculate peptide dosages
Learning how to calculate a peptide dose? Use our beginner-friendly peptide dosage, blend, and accumulation calculators. Enter vial size, reconstitution volume, and target dose to get exact draw volumes instantly. No guesswork, just clear guidance that helps prevent common mistakes.
