Longevity
Crystagen
Immunity
3
Amino acids
Molecular weight
Peptide
Type
Crystagen is a synthetic tripeptide bioregulator (Glu-Asp-Pro) derived from thymic extract that modulates immune system function through epigenetic regulation of immune cell gene expression. It influences T-lymphocyte, B-lymphocyte, and macrophage activity, normalizing immune function in aging populations. Used primarily in longevity and immune support research, it is a Russian-developed peptide bioregulator with decades of observational data but no formal Western clinical trials.
Top researched benefits
Overview of Crystagen
Crystagen enters the cell nucleus and interacts directly with DNA and histones to modulate gene expression in immune cells, upregulating heat-shock protein (HSP) synthesis, regulating cytokine production (including IL-6), and stimulating normal lymphocyte proliferation while inhibiting tumor cell lines. This epigenetic mechanism restores thymic microenvironment integrity and T-cell differentiation capacity that declines with age.
immune support
- Normalizes immunogram in elderly patients with impaired immunity.
- Restores immune function after infectious diseases.
- Helps normalize immunity after radiation and chemotherapy exposure.
stress resistance
- Increases stress resistance and reduces respiratory infections in athletes.
- Doubles expression of heat-shock protein gene HSPA1A.
antitumor
- Inhibits proliferation of K-562 human erythromyelosis tumor cells.
Typical Dose
10–20mg per day
Frequency
once daily
Cycle Duration
10-day course, repeated 2–3 times per year
Storage
Lyophilized: 2–8°C protected from light. Reconstituted with bacteriostatic water: 2–8°C for up to 28 days; do not freeze once reconstituted.
Chemical Makeup
Key benefits
Normalized immune function (immunogram) in 82% of elderly patients versus 56% in control groups in observational studies
Doubles heat-shock protein (HSP) gene expression in athletes, strengthening immune resilience during heavy training stress
Reduces incidence of respiratory viral infections in athletes by normalizing IL-6 expression and boosting innate immune defenses
Supports immune recovery following chemotherapy or radiotherapy by restoring lymphocyte populations
Community interest
This peptide is still gaining traction in the community.
Immune Bioregulator | Immune Function & Longevity
This overview is informational and based on aggregated descriptions from studies and user reports.
Was it helpful?YesNoCrystagen Molecular Information
View the scientifc details of Crystagen.
3
Amino Acids
Crystagen
Glu
Glu
Position 1
Asp
Asp
Position 2
Pro
Pro
Position 3
Molecular Weight
359.33DaChain Length
3Amino AcidsType
PeptideCrystagen Protocols
Subcutaneous injection provides the highest bioavailability for Crystagen's thymus-derived tripeptide (EDP), bypassing gastrointestinal degradation entirely. Inject into abdominal fatty tissue or the front of the thigh at bedtime, at least 2 hours after the last meal, across 5-20 day courses repeated 2-4 times per year.
| Goal | Dosage | Frequency | Route |
|---|---|---|---|
| First-use safety evaluation before committing to a full course | 0.5 | 1 day range | SubQ |
| General immune normalization and thymus support in healthy adults | 1 | 1 day range | SubQ |
| Accelerated immune response support during active viral or bacterial infection | 1 | 1 day range | SubQ |
| Significant immunodeficiency or post-chemotherapy immune reconstruction | 3.5 | 1 day range | SubQ |
| Long-term anti-aging immune maintenance in adults over 40, repeated 2-4 times per year | 1 | 5 week range | SubQ |
Reconstitution Instructions
Materials needed:
Steps to reconstitute
- Remove the Crystagen 20mg lyophilized vial from refrigeration and allow it to reach room temperature for 5-10 minutes.
- Draw 2 mL of bacteriostatic water into a fresh insulin syringe.
- Insert the needle through the rubber stopper and inject BAC water slowly down the inner wall of the vial — never directly onto the powder.
- Gently swirl the vial in a circular motion until the powder is fully dissolved — do not shake.
- Allow the solution to rest for 2-3 minutes; inspect for clarity and discard if cloudy or particulate.
- Draw the target dose into a fresh insulin syringe and remove air bubbles (at 2 mL total volume: 10 units = 1 mg; 5 units = 0.5 mg).
- Cleanse the injection site (abdomen at least 5 cm from navel, front of thigh, or back of upper arm) with an alcohol swab and allow to dry.
- Pinch a fold of skin, insert the needle at a 45-degree angle, and inject slowly over 5-10 seconds.
- Withdraw the needle, apply gentle pressure with a clean swab, and rotate to a different site for the next injection.
- Store the reconstituted vial refrigerated at 2-8°C and use within 14-28 days.
Crystagen Cycle
The Crystagen Cycle section explains how long a typical cycle lasts and what to expect during each phase. Over time, your body can become less responsive with continuous use.
Taking breaks between cycles may help maintain effectiveness and support better overall results.
- Week 0-0
- Immune activation and gene expression modulation begins
- Week 0-0
- Effects persist due to epigenetic changes
- Week 0-0
- Measurable immune function improvements
- Week 0-0
- Cumulative benefits with periodic cycles
Dosing tools
Crystagen Peptide Dosage Calculator
Calculate peptide doses with our visual syringe guide.
mg
Enter the total amount of peptide in the vial in milligrams (as stated on the label).
The dose you want to inject per administration, in mcg or mg.
1,000 mcg = 1 mg
1 mL
2 mL
3 mL
5 mL
Custom
Volume of bacteriostatic water you add to reconstitute the powder. Use BAC water for preservation.
Injection Results
Based on your vial and dilution inputs.
Crystagen
SINGLE COMPOUNDVolume per injection
0.05
mL
Concentration
10.00
mg/mL
Doses per vial
20
doses
Total injections per vial
20 injections
How it works
Based on a 10 mg Crystagen vial diluted with 1 mL of bacteriostatic water, each 500 mcg injection equals 0.05 mL.
1mL / 100 units
5 units
0.050 mL
Reference Guide
Dosing Cycle
- Peptide
- Crystagen
- Dosing
- 10–20mg per day
- Dosing Frequency
- once daily
- Cycle Duration
- 10-day course, repeated 2–3 times per year
- Storage
- Lyophilized: 2–8°C protected from light. Reconstituted with bacteriostatic water: 2–8°C for up to 28 days; do not freeze once reconstituted.
Note: Triple agonist; microdose for fewer side effects
Reconstitution Tips
- Use bacteriostatic water (BAC) — contains 0.9% benzyl alcohol for preservation
- Inject water slowly — aim down the vial wall, not directly onto powder
- Never shake — gently swirl or roll the vial until dissolved
- Store properly — refrigerate at 2-8°C after reconstitution
- Use within 28 days — most reconstituted peptides remain stable for about 4 weeks
- Keep sterile — always clean vial tops with alcohol before drawing
Peptide Interactions
Research suggestions of Crystagen interactions with other common peptides and substances.
Longevity
epi
Immunity
Epitalon
SYNERGISTIC
Often combined in comprehensive anti-aging Khavinson protocols.
Longevity
thy
Immunity
Thymalin
SYNERGISTIC
Crystagen (EDP) is derived from thymalin; complementary immune effects.
Longevity
vil
Immunity
Vilon
SYNERGISTIC
Both have immunoprotecting properties; different mechanisms complement each other.
Side effects
Contraindications
Pregnancy or breastfeeding
Active autoimmune flares (consult physician)
Known hypersensitivity
Stop signs
Allergic reactions
Unusual immune symptoms
Bad signs
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Frequently asked questions
What is a peptide dosage calculator?
A peptide dosage calculator is a free tool that converts your vial size, bacteriostatic water volume, and target dose into an exact syringe draw volume. Instead of doing the reconstitution math by hand, you enter three inputs and instantly get the concentration of your solution and how many milliliters or syringeunits to draw. This calculator works for single peptide compounds and multi-peptide blends.
How do I calculate peptide dosage from a vial?
To calculate your peptide dose, divide the total peptide content of your vial in micrograms by the volume of bacteriostatic water you added in milliliters. This gives you your solution concentration in mcg/mL. Then divide your target dose by that concentration to get your draw volume. For example, a 5mg (5,000 mcg) vial reconstituted with 2mL of BAC water gives a concentration of 2,500 mcg/mL. A 250 mcg dose would require drawing 0.1mL. This calculator automates all of those steps instantly.
How much Bacteriostatic water should I add to a peptide vial?
Most people add 2mL to 3mL of bacteriostatic water per vial, but the right amount depends on the dose you want to draw and the syringe size you are using. Adding 1mL to a 5mg vial gives you a concentration of 5,000 mcg/mL, making each dose very small in volume. Adding 2mL gives you 2,500 mcg/mL, which is easier to measure on a standard insulin syringe. A general guideline is to choose a volume that puts your typical dose somewhere between 10 and 30 units on a U-100 syringe. Use the calculator above to test different water volumes and find what works for your dose.
How are peptides different from proteins?
Both are made of amino acids, but peptides are much smaller than proteins. Because of their tiny size, peptides can act like tiny messengers in the body, sending specific signals to your cells to tell them exactly what to do.
CrystagenResearch References
Crystagen is a preclinical compound
Crystagen
Crystagen is a preclinical compound
The Use of Thymalin for Immunocorrection and Molecular Aspects of Biological Activity
EDP (crystagen) identified in thymalin; regulates gene expression, HSP synthesis, cytokines, and cell differentiation.
n.d.
Crystagen in Elderly Patients with Impaired Immunity
Crystagen normalized immunogram in 82% of patients vs 56% in control group.
n.d.
Peptide Bioregulators in Athletes
Crystagen doubled HSP gene expression, normalized IL-6, and reduced respiratory infections in athletes.
n.d.
EDP Peptide Effects on Lymphocyte Proliferation
EDP enhances normal lymphocyte proliferation while inhibiting K-562 tumor cell proliferation.
n.d.
Calculate peptide dosages
Learning how to calculate a peptide dose? Use our beginner-friendly peptide dosage, blend, and accumulation calculators. Enter vial size, reconstitution volume, and target dose to get exact draw volumes instantly. No guesswork, just clear guidance that helps prevent common mistakes.
