Cognitive
VIP
Immunity
3
Amino acids
Molecular weight
Peptide
Type
Vasoactive Intestinal Peptide (VIP) is an endogenous 28-amino acid neuropeptide that binds VPAC1 and VPAC2 receptors to produce vasodilation, immune tolerance, and neuroprotection. It has been investigated in experimental contexts for pulmonary arterial hypertension, CIRS (chronic inflammatory response syndrome), sarcoidosis, and neurological disorders. Researchers and clinicians use VIP for its potent anti-inflammatory effects without suppressing immune function.
Top researched benefits
Overview of VIP
VIP binds VPAC1 and VPAC2 G-protein-coupled receptors of the secretin receptor family, activating adenylyl cyclase to elevate intracellular cAMP and PKA activity. This cascade phosphorylates CREB and other transcription factors, suppressing pro-inflammatory cytokines (TNF-α, IL-6, IL-12), upregulating anti-inflammatory mediators (IL-10, TGF-β), and promoting regulatory T-cell differentiation.
cardiovascular
- VIP inhalation shows striking efficacy with increased mixed venous oxygen saturation and exercise capacity.
- Dilates peripheral blood vessels through NO-dependent mechanisms above 100 pmol doses.
- Coronary vasodilation with positive inotropic and chronotropic effects on the heart.
neurological
- Promising therapeutic target for Alzheimer's, Parkinson's, and other neurological disorders.
- Potential therapeutic target being researched for ASD.
- Produced in suprachiasmatic nuclei; involved in circadian regulation.
metabolic and immune
- Promotes insulin secretion in glucose-dependent manner via VPAC2; low hypoglycemia risk.
- Potent anti-inflammatory effects useful in IBD and autoimmune conditions.
- Therapeutic potential for pulmonary and systemic sarcoidosis.
Typical Dose
50mcg per administration
Frequency
4 times daily (intranasal) or twice daily (subcutaneous)
Cycle Duration
8 weeks on, 8 weeks off
Storage
Lyophilized: store at -20°C, stable up to 24 months. Reconstituted: refrigerate at 2–8°C, use within 14–30 days. Avoid freeze-thaw cycles; discard if left at room temperature for more than 2–3 hours.
Chemical Makeup
Key benefits
Reduces pulmonary arterial pressure and improves exercise capacity in pulmonary hypertension patients via potent vasodilation of pulmonary vasculature
Suppresses pro-inflammatory cytokines (TNF-α, IL-6, IL-12) and promotes regulatory T-cell generation, providing immune balance without immunosuppression
Protects neurons from degeneration in models of Alzheimer's and Parkinson's disease by stimulating glial production of neurotrophic factors and inhibiting neuroinflammation
Resolves chronic inflammatory response in CIRS patients by restoring cytokine balance and reducing TGF-β1-driven fibrosis
Community interest
This peptide is still gaining traction in the community.
Neuropeptide | Anti-Inflammatory & Immune Modulation
This overview is informational and based on aggregated descriptions from studies and user reports.
Was it helpful?YesNoVIP Molecular Information
View the scientifc details of VIP.
3
Amino Acids
VIP
His
His
Position 1
Trp
Trp
Position 2
Arg
Arg
Position 3
Molecular Weight
3325.8DaChain Length
3Amino AcidsType
PeptideVIP Protocols
Subcutaneous injection is a secondary delivery route for VIP used when intranasal access is unavailable or when systemic dosing is preferred. VIP (28 amino acids, ~3.3 kDa) is supplied as a lyophilized powder requiring reconstitution with bacteriostatic water; injection is typically administered into the abdomen or thigh.
| Goal | Dosage | Frequency | Route |
|---|---|---|---|
| Reduce systemic inflammatory markers and support immune regulation through twice-daily subcutaneous VIP dosing at the standard research protocol level | 50 | 2 day range | SubQ |
| Suppress pro-inflammatory cytokine signaling and promote T-regulatory cell activity in autoimmune conditions via once-daily subcutaneous administration | 100 | 1 day range | SubQ |
| Normalize elevated inflammatory biomarkers including C4a, TGF-B1, and MMP-9 as part of a comprehensive CIRS treatment protocol via subcutaneous administration | 50 | 1 day range | SubQ |
Reconstitution Instructions
Materials needed:
Steps to reconstitute
- Allow the VIP vial and bacteriostatic water vial to reach room temperature before handling
- Draw 5 mL of bacteriostatic water into a syringe using an 18–21 gauge drawing needle
- Inject the bacteriostatic water slowly down the inside wall of the 5 mg VIP vial — never spray directly onto the lyophilized powder cake
- Gently swirl the vial in a slow circular motion until the powder fully dissolves — do not shake; shaking can degrade the peptide
- The resulting concentration is 1,000 mcg/mL (1 mg/mL); a 50 mcg dose equals 5 units on a 100-unit insulin syringe
- Label the reconstituted vial with the date; store refrigerated at 2–8°C and use within 28 days
- Wipe the injection site (abdomen, thigh, or upper arm) with an alcohol swab and allow to dry
- Pinch the skin, insert the insulin syringe needle at a 45–90 degree angle into the subcutaneous fatty tissue, and inject slowly
- Rotate injection sites with each dose to prevent lipodystrophy
VIP Cycle
The VIP Cycle section explains how long a typical cycle lasts and what to expect during each phase. Over time, your body can become less responsive with continuous use.
Taking breaks between cycles may help maintain effectiveness and support better overall results.
- Week 0-0
- Rapid vasodilation and hemodynamic effects
- Week 0-0
- Anti-inflammatory signaling activated
- Week 0-0
- Cumulative effects on inflammation and immune balance
- Week 0-0
- Sustained benefits with regular administration
Dosing tools
VIP Peptide Dosage Calculator
Calculate peptide doses with our visual syringe guide.
mg
Enter the total amount of peptide in the vial in milligrams (as stated on the label).
The dose you want to inject per administration, in mcg or mg.
1,000 mcg = 1 mg
1 mL
2 mL
3 mL
5 mL
Custom
Volume of bacteriostatic water you add to reconstitute the powder. Use BAC water for preservation.
Injection Results
Based on your vial and dilution inputs.
VIP
SINGLE COMPOUNDVolume per injection
0.05
mL
Concentration
10.00
mg/mL
Doses per vial
20
doses
Total injections per vial
20 injections
How it works
Based on a 10 mg VIP vial diluted with 1 mL of bacteriostatic water, each 500 mcg injection equals 0.05 mL.
1mL / 100 units
5 units
0.050 mL
Reference Guide
Dosing Cycle
- Peptide
- VIP
- Dosing
- 50mcg per administration
- Dosing Frequency
- 4 times daily (intranasal) or twice daily (subcutaneous)
- Cycle Duration
- 8 weeks on, 8 weeks off
- Storage
- Lyophilized: store at -20°C, stable up to 24 months. Reconstituted: refrigerate at 2–8°C, use within 14–30 days. Avoid freeze-thaw cycles; discard if left at room temperature for more than 2–3 hours.
Note: Triple agonist; microdose for fewer side effects
Reconstitution Tips
- Use bacteriostatic water (BAC) — contains 0.9% benzyl alcohol for preservation
- Inject water slowly — aim down the vial wall, not directly onto powder
- Never shake — gently swirl or roll the vial until dissolved
- Store properly — refrigerate at 2-8°C after reconstitution
- Use within 28 days — most reconstituted peptides remain stable for about 4 weeks
- Keep sterile — always clean vial tops with alcohol before drawing
Peptide Interactions
Research suggestions of VIP interactions with other common peptides and substances.
Side effects
Contraindications
Severe hypotension
VIPoma or related tumors
Pregnancy or breastfeeding
Severe cardiac conditions
Stop signs
Severe hypotension
Allergic reaction symptoms
Severe diarrhea
Cardiac arrhythmias
Bad signs
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Frequently asked questions
What is a peptide dosage calculator?
A peptide dosage calculator is a free tool that converts your vial size, bacteriostatic water volume, and target dose into an exact syringe draw volume. Instead of doing the reconstitution math by hand, you enter three inputs and instantly get the concentration of your solution and how many milliliters or syringeunits to draw. This calculator works for single peptide compounds and multi-peptide blends.
How do I calculate peptide dosage from a vial?
To calculate your peptide dose, divide the total peptide content of your vial in micrograms by the volume of bacteriostatic water you added in milliliters. This gives you your solution concentration in mcg/mL. Then divide your target dose by that concentration to get your draw volume. For example, a 5mg (5,000 mcg) vial reconstituted with 2mL of BAC water gives a concentration of 2,500 mcg/mL. A 250 mcg dose would require drawing 0.1mL. This calculator automates all of those steps instantly.
How much Bacteriostatic water should I add to a peptide vial?
Most people add 2mL to 3mL of bacteriostatic water per vial, but the right amount depends on the dose you want to draw and the syringe size you are using. Adding 1mL to a 5mg vial gives you a concentration of 5,000 mcg/mL, making each dose very small in volume. Adding 2mL gives you 2,500 mcg/mL, which is easier to measure on a standard insulin syringe. A general guideline is to choose a volume that puts your typical dose somewhere between 10 and 30 units on a U-100 syringe. Use the calculator above to test different water volumes and find what works for your dose.
How are peptides different from proteins?
Both are made of amino acids, but peptides are much smaller than proteins. Because of their tiny size, peptides can act like tiny messengers in the body, sending specific signals to your cells to tell them exactly what to do.
VIPResearch References
VIP is an experimental compound
VIP
VIP is an experimental compound
VIP as New Drug for Treatment of Primary Pulmonary Hypertension
VIP inhalation showed striking efficacy with increased mixed venous oxygen saturation and exercise capacity.
n.d.
Therapeutic Potential of VIP and VPAC2 in Type 2 Diabetes
VIP promotes glucose-dependent insulin secretion via VPAC2, reducing hypoglycemia risk.
n.d.
Therapeutic Potential of VIP in Neurological Disorders
VIP and receptors are promising therapeutic targets for AD, PD, and autism spectrum disorders.
n.d.
VIP Structure and Function for Therapeutic Applications
Comprehensive review of VIP receptor signaling and therapeutic applications.
n.d.
Calculate peptide dosages
Learning how to calculate a peptide dose? Use our beginner-friendly peptide dosage, blend, and accumulation calculators. Enter vial size, reconstitution volume, and target dose to get exact draw volumes instantly. No guesswork, just clear guidance that helps prevent common mistakes.
