Weight
Sermorelin
Growth
29
Amino acids
Molecular weight
Peptide
Type
Sermorelin is a synthetic 29-amino acid peptide corresponding to the active N-terminal fragment of endogenous growth hormone-releasing hormone (GHRH). It stimulates the anterior pituitary gland to produce and secrete endogenous growth hormone in a physiologically pulsatile pattern, preserving natural feedback regulation. Originally FDA-approved as Geref for pediatric GH deficiency diagnosis, the commercial product was voluntarily withdrawn from the US market in 2002; it is now available only through compounding pharmacies for off-label adult anti-aging and body composition use.
Top researched benefits
Overview of Sermorelin
Sermorelin binds to GHRH receptors on pituitary somatotroph cells and activates adenylyl cyclase via Gs-protein coupling, increasing intracellular cAMP and stimulating GH gene transcription and vesicular GH release. Because it acts through the hypothalamic-pituitary axis, somatostatin-mediated negative feedback remains intact, preserving natural pulsatile GH secretion.
muscle growth
- 1.26kg lean mass increase documented in elderly men with improved muscle strength tests.
- Endogenous IGF-1 stimulation drives protein synthesis and muscle growth.
- Enhanced recovery through physiological GH stimulation.
antiaging
- Doubles 12-hour GH release in elderly subjects over 6 weeks.
- Decreased adiposity and improved lean mass distribution.
- Improved skin thickness and quality.
hormonal
- Preserves natural axis function without suppression.
- Maintains physiological GH release patterns.
Typical Dose
300 mcg per injection
Frequency
once nightly at bedtime
Cycle Duration
3–6 months on, 1–2 months off
Storage
Lyophilized: 2–8°C in dry, dark conditions (or -20°C for long-term). Reconstituted with bacteriostatic water: 2–8°C for up to 28 days; with sterile water: use within 7–14 days.
Chemical Makeup
Key benefits
Stimulates endogenous pulsatile GH release without suppressing the hypothalamic-pituitary axis or triggering negative feedback shutdown
Increases lean muscle mass and reduces visceral adipose tissue by elevating IGF-1 levels downstream of GH secretion
Improves sleep quality and duration, particularly deep slow-wave sleep, due to GH release patterns that peak during early-night sleep cycles
Enhances skin collagen and elastin synthesis, reducing the appearance of fine lines and improving dermal hydration over 3–6 months
Community interest
This peptide is still gaining traction in the community.
GHRH Analog | GH Stimulation & Anti-Aging
This overview is informational and based on aggregated descriptions from studies and user reports.
Was it helpful?YesNoSermorelin Molecular Information
View the scientifc details of Sermorelin.
29
Amino Acids
Sermorelin
Tyr
Tyr
Position 1
Ala
Ala
Position 2
Asp
Asp
Position 3
Ala
Ala
Position 4
?
?
Position 5
Phe
Phe
Position 6
?
?
Position 7
Asn
Asn
Position 8
Ser
Ser
Position 9
Tyr
Tyr
Position 10
Arg
Arg
Position 11
Lys
Lys
Position 12
Val
Val
Position 13
Leu
Leu
Position 14
Gly
Gly
Position 15
Gln
Gln
Position 16
Leu
Leu
Position 17
Ser
Ser
Position 18
Ala
Ala
Position 19
Arg
Arg
Position 20
Lys
Lys
Position 21
Leu
Leu
Position 22
Leu
Leu
Position 23
Gln
Gln
Position 24
Asp
Asp
Position 25
?
?
Position 26
Met
Met
Position 27
Ser
Ser
Position 28
Arg
Arg
Position 29
Molecular Weight
3358DaChain Length
29Amino AcidsType
PeptideSermorelin Protocols
Subcutaneous injection is the primary and most effective route for Sermorelin, delivering near-complete bioavailability by stimulating pituitary somatotroph cells to release growth hormone. Injections must be administered fasted — elevated blood glucose causes hyperglycemia-driven somatostatin release and direct insulin signaling on somatotrophs that blunts the GHRH-receptor response, making a fasted bedtime injection uniquely critical to Sermorelin's mechanism.
| Goal | Dosage | Frequency | Route |
|---|---|---|---|
| Anti-aging and general wellness | 200 | 1 day range | SubQ |
| Muscle preservation and growth | 300 | 1 day range | SubQ |
| Weight and body composition management | 300 | 1 day range | SubQ |
| Sleep optimization | 250 | 1 day range | SubQ |
| Post-injury and surgical recovery | 300 | 1 day range | SubQ |
| GH deficiency therapy (adult onset) | 500 | 1 day range | SubQ |
Reconstitution Instructions
Materials needed:
Steps to reconstitute
- Wash hands thoroughly with soap and water before handling any vials or syringes
- Swab the rubber stopper of both the Sermorelin vial and the BAC water vial with a fresh alcohol wipe and allow to air dry
- Draw air into the syringe equal to the volume of BAC water you will add (e.g., 2 mL for a 6 mg vial)
- Inject that air into the BAC water vial to create positive pressure, then draw out the required BAC water volume
- Insert the needle into the Sermorelin powder vial and inject the BAC water slowly down the inner glass wall — never directly onto the powder
- Remove the needle and gently swirl the vial in slow circular motions until the powder is fully dissolved — do not shake
- Store reconstituted Sermorelin refrigerated at 2–8°C; use within 28–90 days depending on compounding pharmacy guidance
- Before each injection, swab the chosen injection site (abdomen or thigh) with an alcohol wipe and allow to dry
- Pinch a fold of skin at the injection site, insert the insulin needle at a 45–90 degree angle, and inject slowly
- Release the skin fold, withdraw the needle, and apply gentle pressure with a clean swab — do not rub
- Rotate injection sites with each dose to prevent lipodystrophy
Sermorelin Cycle
The Sermorelin Cycle section explains how long a typical cycle lasts and what to expect during each phase. Over time, your body can become less responsive with continuous use.
Taking breaks between cycles may help maintain effectiveness and support better overall results.
- Week 1-2
- IGF-1 elevation begins; improved sleep quality and recovery
- Week 2-4
- Body composition changes begin; increased energy and well-being
- Week 4-8
- Visible muscle tone improvements; fat reduction; skin quality enhancement
- Week 8-12
- Sustained improvements; optimal IGF-1 elevation
- Month 3-6
- Maximum benefits: muscle growth, fat loss, anti-aging effects
Dosing tools
Sermorelin Peptide Dosage Calculator
Calculate peptide doses with our visual syringe guide.
mg
Enter the total amount of peptide in the vial in milligrams (as stated on the label).
The dose you want to inject per administration, in mcg or mg.
1,000 mcg = 1 mg
1 mL
2 mL
3 mL
5 mL
Custom
Volume of bacteriostatic water you add to reconstitute the powder. Use BAC water for preservation.
Injection Results
Based on your vial and dilution inputs.
Sermorelin
SINGLE COMPOUNDVolume per injection
0.05
mL
Concentration
10.00
mg/mL
Doses per vial
20
doses
Total injections per vial
20 injections
How it works
Based on a 10 mg Sermorelin vial diluted with 1 mL of bacteriostatic water, each 500 mcg injection equals 0.05 mL.
1mL / 100 units
5 units
0.050 mL
Reference Guide
Dosing Cycle
- Peptide
- Sermorelin
- Dosing
- 300 mcg per injection
- Dosing Frequency
- once nightly at bedtime
- Cycle Duration
- 3–6 months on, 1–2 months off
- Storage
- Lyophilized: 2–8°C in dry, dark conditions (or -20°C for long-term). Reconstituted with bacteriostatic water: 2–8°C for up to 28 days; with sterile water: use within 7–14 days.
Note: Triple agonist; microdose for fewer side effects
Reconstitution Tips
- Use bacteriostatic water (BAC) — contains 0.9% benzyl alcohol for preservation
- Inject water slowly — aim down the vial wall, not directly onto powder
- Never shake — gently swirl or roll the vial until dissolved
- Store properly — refrigerate at 2-8°C after reconstitution
- Use within 28 days — most reconstituted peptides remain stable for about 4 weeks
- Keep sterile — always clean vial tops with alcohol before drawing
Peptide Interactions
Research suggestions of Sermorelin interactions with other common peptides and substances.
Weight
ghr
Growth
GHRP-2
SYNERGISTIC
Combined GHRH+GHRP-2 produces 54-fold GH increases compared to 20-fold with GHRH alone.
Weight
ipa
Growth
Ipamorelin
SYNERGISTIC
Excellent combination producing 3-5 fold increases in GH release.
Side effects
Contraindications
Active malignancy
Pituitary tumors
Pregnancy
Stop signs
Signs of pituitary tumor growth (headaches, vision changes)
Severe injection site reactions or generalized allergic responses
Uncontrolled diabetes or significant glucose intolerance
New onset or worsening malignancy symptoms
Bad signs
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Frequently asked questions
What is a peptide dosage calculator?
A peptide dosage calculator is a free tool that converts your vial size, bacteriostatic water volume, and target dose into an exact syringe draw volume. Instead of doing the reconstitution math by hand, you enter three inputs and instantly get the concentration of your solution and how many milliliters or syringeunits to draw. This calculator works for single peptide compounds and multi-peptide blends.
How do I calculate peptide dosage from a vial?
To calculate your peptide dose, divide the total peptide content of your vial in micrograms by the volume of bacteriostatic water you added in milliliters. This gives you your solution concentration in mcg/mL. Then divide your target dose by that concentration to get your draw volume. For example, a 5mg (5,000 mcg) vial reconstituted with 2mL of BAC water gives a concentration of 2,500 mcg/mL. A 250 mcg dose would require drawing 0.1mL. This calculator automates all of those steps instantly.
How much Bacteriostatic water should I add to a peptide vial?
Most people add 2mL to 3mL of bacteriostatic water per vial, but the right amount depends on the dose you want to draw and the syringe size you are using. Adding 1mL to a 5mg vial gives you a concentration of 5,000 mcg/mL, making each dose very small in volume. Adding 2mL gives you 2,500 mcg/mL, which is easier to measure on a standard insulin syringe. A general guideline is to choose a volume that puts your typical dose somewhere between 10 and 30 units on a U-100 syringe. Use the calculator above to test different water volumes and find what works for your dose.
How are peptides different from proteins?
Both are made of amino acids, but peptides are much smaller than proteins. Because of their tiny size, peptides can act like tiny messengers in the body, sending specific signals to your cells to tell them exactly what to do.
SermorelinResearch References
Sermorelin is an experimental compound
Sermorelin
Sermorelin is an experimental compound
FDA Approval Study - Geref International Study Group Trial
Pivotal trial with 110 prepubertal GH-deficient children demonstrating sustained efficacy through 36 months. Height velocity increased from 4.1 to 8.0 cm/year.
1996
Adult Anti-Aging Study - Corpas et al.
Landmark study demonstrating reversal of age-related GH/IGF-1 decline in 60-78 year old males. Doubled 12-hour GH release over 6 weeks.
1992
Pharmacokinetic Profile
Comprehensive analysis showing 11-12 minute half-life and 6% bioavailability for subcutaneous administration.
1996
Body Composition in Elderly
1.26kg lean mass increase with improved muscle strength; decreased systolic BP.
1992
Nasal Administration Study
69% patient preference over injection with reliable therapeutic response at 30+ mcg/kg.
1990
Calculate peptide dosages
Learning how to calculate a peptide dose? Use our beginner-friendly peptide dosage, blend, and accumulation calculators. Enter vial size, reconstitution volume, and target dose to get exact draw volumes instantly. No guesswork, just clear guidance that helps prevent common mistakes.
