Weight
CJC-1295 with DAC
Growth
29
Amino acids
Molecular weight
Peptide
Type
CJC-1295 with DAC (Drug Affinity Complex) is a long-acting synthetic analog of growth hormone-releasing hormone (GHRH) that stimulates the anterior pituitary to secrete endogenous growth hormone. The DAC modification enables covalent binding to circulating albumin, extending its half-life to approximately 6–8 days and allowing once- to twice-weekly dosing. It is used in research and clinical contexts targeting GH deficiency, body composition improvement, and anti-aging applications.
Top researched benefits
Overview of CJC-1295 with DAC
CJC-1295 with DAC binds to GHRH receptors on somatotroph cells in the anterior pituitary, activating cAMP-dependent PKA signaling cascades that drive GH gene transcription and pulsatile GH release. The DAC moiety forms a reversible covalent bond with endogenous serum albumin upon injection, creating a slow-release depot that sustains elevated GH and downstream IGF-1 levels for up to 9–11 days per dose.
growth hormone
- Continuous growth hormone release for 6-8 days per injection.
- Significantly elevates IGF-1 levels for extended periods.
- Weekly dosing ideal for simple administration.
body composition
- Continuous GH elevation promotes lipolysis and fat metabolism.
- Sustained anabolic environment supports muscle protein synthesis.
antiaging
- Accelerated healing and recovery from exercise or injury.
Typical Dose
300–1000mcg per injection (titrated)
Frequency
twice weekly
Cycle Duration
8–12 weeks on, followed by a break
Storage
Lyophilized: -20°C for up to 24 months. Reconstituted: 2–8°C for up to 28 days; do not freeze after reconstitution.
Chemical Makeup
Key benefits
Increases plasma GH levels 2- to 10-fold for up to 6 days from a single injection, sustaining physiological GH pulses without exogenous GH administration
Elevates IGF-1 levels by 1.5- to 3-fold for 9–11 days, promoting systemic anabolic signaling for muscle protein synthesis and tissue repair
Supports lean mass accrual by enhancing protein synthesis and reducing fat mass through increased lipolysis driven by elevated GH/IGF-1 signaling
Once- to twice-weekly dosing schedule due to the 6–8 day half-life conferred by albumin binding via the Drug Affinity Complex modification
Community interest
This peptide is still gaining traction in the community.
GHRH Analog | GH Stimulation & Body Composition
This overview is informational and based on aggregated descriptions from studies and user reports.
Was it helpful?YesNoCJC-1295 with DAC Molecular Information
View the scientifc details of CJC-1295 with DAC.
29
Amino Acids
CJC-1295 with DAC
Tyr
Tyr
Position 1
Ala
Ala
Position 2
Asp
Asp
Position 3
Ala
Ala
Position 4
Ile
Ile
Position 5
Phe
Phe
Position 6
Thr
Thr
Position 7
Gln
Gln
Position 8
Ser
Ser
Position 9
Tyr
Tyr
Position 10
Arg
Arg
Position 11
Lys
Lys
Position 12
Val
Val
Position 13
Leu
Leu
Position 14
Ala
Ala
Position 15
Gln
Gln
Position 16
Leu
Leu
Position 17
Ser
Ser
Position 18
Ala
Ala
Position 19
Arg
Arg
Position 20
Lys
Lys
Position 21
Leu
Leu
Position 22
Leu
Leu
Position 23
Gln
Gln
Position 24
Asp
Asp
Position 25
Ile
Ile
Position 26
Leu
Leu
Position 27
Ser
Ser
Position 28
Arg
Arg
Position 29
Molecular Weight
3647.25DaChain Length
29Amino AcidsType
PeptideCJC-1295 with DAC Protocols
Subcutaneous injection is the standard and most well-documented route for CJC-1295 with DAC. Due to its extended half-life of 6–8 days from the DAC albumin-binding modification, once or twice weekly injections into abdominal fat are sufficient to maintain stable GH and IGF-1 elevation.
| Goal | Dosage | Frequency | Route |
|---|---|---|---|
| General Health / Anti-Aging | 1000 | 1 week range | SubQ abdominal fat |
| Fat Loss / Body Composition | 1000 | 2 week range | SubQ abdominal fat |
| Muscle Growth / Recovery | 1000 | 2 week range | SubQ abdominal fat or thigh |
| GH Optimization (Beginner) | 500 | 2 week range | SubQ abdominal fat |
| GH Optimization (Intermediate) | 500 | 2 week range | SubQ abdominal fat |
| Tissue Healing / Injury Repair | 2000 | 1 week range | SubQ |
Reconstitution Instructions
Materials needed:
Steps to reconstitute
- Allow the lyophilized CJC-1295 DAC vial to come to room temperature for 15 minutes before reconstitution
- Wipe the rubber stopper of both the peptide vial and bacteriostatic water vial with an alcohol swab and allow to dry
- Draw 2.0 mL of bacteriostatic water into a 3 mL syringe for a 5 mg vial, or 1.0 mL for a 2 mg vial
- Insert the needle into the peptide vial at an angle and slowly inject the BAC water down the inner glass wall — do not shoot it directly onto the powder
- Gently swirl or roll the vial between your palms until fully dissolved — never shake, as this degrades the peptide
- Confirm the solution is clear and colorless with no visible particles before drawing a dose
- Label the vial with the reconstitution date and concentration; store refrigerated at 2–8°C and use within 4 weeks
- To inject: wipe the injection site with an alcohol swab and allow to dry, then pinch a fold of abdominal skin at least 5 cm from the navel
- Insert a 29–31 gauge insulin syringe at a 45–90 degree angle and depress the plunger slowly
- Withdraw the needle and apply gentle pressure with a clean swab — do not massage the injection site
- Rotate injection sites systematically across the abdomen, thighs, and upper arms to prevent site irritation
CJC-1295 with DAC Cycle
The CJC-1295 with DAC Cycle section explains how long a typical cycle lasts and what to expect during each phase. Over time, your body can become less responsive with continuous use.
Taking breaks between cycles may help maintain effectiveness and support better overall results.
- Week 0-0
- Possible water retention, improved sleep, increased appetite
- Week 2-3
- Noticeable recovery improvements, some report joint discomfort
- Week 4-6
- Visible body composition changes, increased vascularity
- Week 6-8
- Continued improvements but watch for desensitization signs
- Week 0-0
- Most gains maintained; IGF-1 returns to baseline in 2-3 weeks
Dosing tools
CJC-1295 with DAC Peptide Dosage Calculator
Calculate peptide doses with our visual syringe guide.
mg
Enter the total amount of peptide in the vial in milligrams (as stated on the label).
The dose you want to inject per administration, in mcg or mg.
1,000 mcg = 1 mg
1 mL
2 mL
3 mL
5 mL
Custom
Volume of bacteriostatic water you add to reconstitute the powder. Use BAC water for preservation.
Injection Results
Based on your vial and dilution inputs.
CJC-1295 with DAC
SINGLE COMPOUNDVolume per injection
0.05
mL
Concentration
10.00
mg/mL
Doses per vial
20
doses
Total injections per vial
20 injections
How it works
Based on a 10 mg CJC-1295 with DAC vial diluted with 1 mL of bacteriostatic water, each 500 mcg injection equals 0.05 mL.
1mL / 100 units
5 units
0.050 mL
Reference Guide
Dosing Cycle
- Peptide
- CJC-1295 with DAC
- Dosing
- 300–1000mcg per injection (titrated)
- Dosing Frequency
- twice weekly
- Cycle Duration
- 8–12 weeks on, followed by a break
- Storage
- Lyophilized: -20°C for up to 24 months. Reconstituted: 2–8°C for up to 28 days; do not freeze after reconstitution.
Note: Triple agonist; microdose for fewer side effects
Reconstitution Tips
- Use bacteriostatic water (BAC) — contains 0.9% benzyl alcohol for preservation
- Inject water slowly — aim down the vial wall, not directly onto powder
- Never shake — gently swirl or roll the vial until dissolved
- Store properly — refrigerate at 2-8°C after reconstitution
- Use within 28 days — most reconstituted peptides remain stable for about 4 weeks
- Keep sterile — always clean vial tops with alcohol before drawing
Peptide Interactions
Research suggestions of CJC-1295 with DAC interactions with other common peptides and substances.
Weight
cjc
Growth
CJC-1295 (without DAC)
AVOID
Never combine different variants; choose based on desired release pattern.
Weight
ipa
Growth
Ipamorelin
MONITOR
Continuous GH elevation may reduce synergistic benefits of pulsatile protocols.
Weight
mk6
Growth
MK-677
AVOID
Both provide continuous GH elevation; combination risks excessive levels.
Weight
tesa
Growth
Tesamorelin
AVOID
Both are GHRH analogs; no additional benefit and potential receptor competition.
Side effects
Avoid: Do not take CJC-1295 with DAC with CJC-1295 (without DAC), HGH, MK-677, Tesamorelin.
Monitor: Be careful when combining CJC-1295 with DAC with GHRP-6, Ipamorelin, Sermorelin.
Contraindications
Diabetes history
Cancer history
Predisposed sleep apnea
Stop signs
Severe joint pain or carpal tunnel syndrome
Excessive water retention affecting daily life
Numbness or tingling in extremities
Significant blood glucose dysregulation
Signs of acromegaly (jaw growth, hand/feet enlargement)
Persistent lethargy indicating adrenal effects
Bad signs
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Frequently asked questions
What is a peptide dosage calculator?
A peptide dosage calculator is a free tool that converts your vial size, bacteriostatic water volume, and target dose into an exact syringe draw volume. Instead of doing the reconstitution math by hand, you enter three inputs and instantly get the concentration of your solution and how many milliliters or syringeunits to draw. This calculator works for single peptide compounds and multi-peptide blends.
How do I calculate peptide dosage from a vial?
To calculate your peptide dose, divide the total peptide content of your vial in micrograms by the volume of bacteriostatic water you added in milliliters. This gives you your solution concentration in mcg/mL. Then divide your target dose by that concentration to get your draw volume. For example, a 5mg (5,000 mcg) vial reconstituted with 2mL of BAC water gives a concentration of 2,500 mcg/mL. A 250 mcg dose would require drawing 0.1mL. This calculator automates all of those steps instantly.
How much Bacteriostatic water should I add to a peptide vial?
Most people add 2mL to 3mL of bacteriostatic water per vial, but the right amount depends on the dose you want to draw and the syringe size you are using. Adding 1mL to a 5mg vial gives you a concentration of 5,000 mcg/mL, making each dose very small in volume. Adding 2mL gives you 2,500 mcg/mL, which is easier to measure on a standard insulin syringe. A general guideline is to choose a volume that puts your typical dose somewhere between 10 and 30 units on a U-100 syringe. Use the calculator above to test different water volumes and find what works for your dose.
How are peptides different from proteins?
Both are made of amino acids, but peptides are much smaller than proteins. Because of their tiny size, peptides can act like tiny messengers in the body, sending specific signals to your cells to tell them exactly what to do.
CJC-1295 with DACResearch References
CJC-1295 with DAC is a phase 2 compound
CJC-1295 with DAC
CJC-1295 with DAC is a phase 2 compound
Prolonged Growth Hormone Elevation with CJC-1295 DAC
12 subjects; sustained GH and IGF-1 elevation for 6-10 days post-injection with peak 2-4x baseline.
2006
Dose-Response Relationship of CJC-1295 DAC
48 subjects, 90 days. Optimal dosing 1-2mg weekly; dose-dependent IGF-1 increases but higher side effects above 2mg.
2008
Long-term Effects on GH/IGF-1 Axis
6-month animal model. Sustained IGF-1 elevation but evidence of reduced GH pulse amplitude suggesting desensitization.
2012
Comparative Pharmacokinetics: DAC vs Non-DAC Variants
DAC: 120-hour half-life vs non-DAC: 30-minute; non-DAC preserves more physiological patterns.
2015
Calculate peptide dosages
Learning how to calculate a peptide dose? Use our beginner-friendly peptide dosage, blend, and accumulation calculators. Enter vial size, reconstitution volume, and target dose to get exact draw volumes instantly. No guesswork, just clear guidance that helps prevent common mistakes.
